Biol. Chem. Research. 48 Isolation of Saccharomyces Spp from Manure of Beef Bali Cattle as a Probiotics Properties and has CMC-ase Activity to Improve Nurient Quality of Rice Bran.

I solation of……………………………….Rice Bran Candraw ati, et al., 20 14 The sam e t hing w as report ed also by Sabini et al. 2000 which st at es t hat t he fungus T. reesei is able t o degrade a polysaccharide mannan mannot riosa, m annobiosa, and monnosa. According to Jaelani et al. 2008, ferment ed palm kernel cake can m arkedly increase t he crude prot ein cont ent compared to palm kernel cake w it hout ferm ent at ion. Table 3 show s t he chem ical composit ion of unferm ent ed rice bran UFR and ferm ent ed rice bran FR ingredient . The digest ibilit y both of crude prot ein, organic m at t er, crude fibre, and met abolizable energy w ere slight ly increased by S.cerevisiae ferm ent at ion. S. cerevisiae can not effect on crude fiber digest ibility of rice bran. Becouse, among t he cell w all polysaccharides of rice bran known as nonst arch polysaccharides NSP are celluloses, pect ins, and oligosaccharides. NSPs can not be degraded enzymit ically in t he digest ive syst em s of t he birds due t o the lacking of enzymes degrading the NSPs in t heir digest ive syst ems Choct , 2002. The digest ibilit y of crude prot ein, crude fiber, and M E w ere slight ly increased by t he ferm ent at ion. These result s indicat ed that carbohydrat es other t han fibres w ere used for m icrobial grow t h Saccharomyces cerevisiae and the reduct ion of nit rogen free ext ract result ed in increased concent ration of t he other com ponent s. Yi et al. l996 report ed t hat supplement at ion of m icrobial in diet s im proved N ret ent ion in broiler chickens and in vit ro digest ibilit y of veget able prot ein. Also, Chen et al. 2005 report ed that addit ion of 0.20 complex probiot ic L.acidophilus and S.cerivisae in basal diet s w ere inceased digest iblit ies of dry matt er. Ferm ent ed of rice bran ingredient by ragi Saccharomyces cereviseae had bet t er digest ibilit ies, because Saccharomyces cereviseae in the gast roint est inal t ract can part of an probiot ics sourches. Also, Piao et al. l999, suggest ed t hat probiot ics in the gast ro intest inal t ract can improve prot ein and energy ret ent ion on t he body of birds. These fungal are effect ive in degrading of t he complex com pounds such as b et a-glucans and arabinoxylans Bedford and Classen, 1992. Bioferm ent at ion of rice bran by Saccharomuces spp.S-6 and S-7 isolat es cult ure had bet t er digest ibility, because Saccharomuces spp culture in the gast ro int est inal t ract can t o be part of probiotic sources. Saccharomyces cerevisiae as part of probiot ic could im prove prot ein and energy ret ent ion in t he gast ro intest inal t ract of the birds Piao et al., l999. Cho et al. 2007 report ed that supplement at ion of m icrobe in diet could improve t he bioavailabilit y of diet ary. Wang et al. 2004 report ed t hat t he inclusion of fiber sources such as w heat bran or pot at o st arch reduced t he m aint enance of energy requirem ent . These fungi are effect ive in degradating the complex compounds such as bet a-glucans and arabinoxylans Dubey, 2006. Chen et al. 2005 report ed that diet ary supplement at ion of complex probiot ic slight ly improved digest ibilit y of nut rient s. Inconsist ent report s about the effect of probiotics m ay be due t o several aspect s such as st rains of bact eria, dose level, diet composition, feeding st rat egy, feed form , and interact ion with other diet ary feed addit ives Chesson, 1994. The high level of non-st arch polysaccharides NSP in rice bran is limit ing it s unrest rict ed use in poult ry feeding. The NSP is know n increasing t he gut viscosit y, reduce nut rient absorption in t he int est ine and affect indirect ly t he grow t h and perform ance of bird Rham es et al., 2006; Cho et al., 2007.

J. Biol. Chem. Research. 48

Vol. 3 1, No. 1: 3 9-52 20 14 I solation of……………………………….Rice Bran Candraw ati, et al., 20 14 M any st udies have clearly demonst rat ed t hat t he addition of probiot ics culture or enzym es t o diet s rich in NSP result ed in a significant reduction of the int est inal viscosit y, enhances energy and prot ein utilization. Wang et al. 2004 report ed that degree of microbial ferm ent at ion in the large int est ine improves t he bioavailabilit y of diet ary. Yi et al. l996 report ed that supplem ent at ion of m icrobial in diet s improved N ret ent ion in broiler chickens and in vit ro digest ibilit y of veget able prot ein. Also, Chen et al. 2005 report ed t hat addit ion of 0.20 complex probiotic L. acidophilus and S. cerivisae in basal diet s increased digest iblit y of DM and CP. Hong et al. 2004 report ed t hat ferment at ion of feed using Aspergilus oryzae increased digest ibilit y of it s DM and CP. The inclusion of soluble diet ary fiber w heat bran increased daily NSP excret ed from feces Wang et al., 2004; Suprapt i et al., 2008, increased bot h of m et abolizable energy and crude prot ein cont ent s of palm kornel meal Jaelani et al., 2008. Chen et al. 2005 report ed that diet ary supplem ent at ion of complex probiot ic slightly improved digest ibilit y of nut rient s. CONCLUSION It w as concluded t hat t here are t wo isolate Sc.S-6 and S-7 w ere isolat ed from manure of beef Bali cat tle sam ples, bot h w ere t he pot ensial as a probiot ics sources and has CM C-ase akct ivit y and it s ut ilizat ion in the rice bran ferm ent at ion could increase nut rient com posit ion and digest ibilit y of rice bran. ACKNOW LEDGEM ENTS Authors are ent irely grat eful for the chem ical analyses of sample by st aff of Nut rit ion Laboratory, Fakult y of Anim al Husbandry, Udayana Universit y, Denpasar-Bali. The authors also w ant t o thank the Ditbinlit abmas, Dirjen Dikt i, Depdiknas in Jakart a for funding t his research. A part of the present st udy w as financially support ed by Ditbinlit abm as, Dirjen Dikt i, Depdiknas in Jakart a. REFERENCES Ahm ad, R. Z. 2005. Used of Kham ir Saccharomyces cerevisiae for feeding. W art azoa Vol. 15 1 : 49-55 Assocciat ion of Official Analyt ical Chem ist s. l994. Official M et hods of Analysis. 15 t h Edition. Associoation of Analyt ical Chemist s, Arlingt on, Virginia pp. 1230 Bach Knudsen, K. E. 2001. The nut ritional significance of “ diet ary fiber” analysis. Anim. Feed Sci. Tech . 90 : 3-20 Bedford, M .R. and H.L. Classen. 1992. Reduct ion int est inal viscosit y through m anipulation of diet ary rye and pentosanase concent rat ion is affect ed t hrough changes in the carbohydrat e com posit ion of t he int est inal equous phase and result in improved w heat s and food conversion efficiency of broiler chicks. J. Nut r. 122:560-569 Bidura, I.G.N.G., D. A. Warm adew i, D.P.M .A. Candraw at i, I. G. A. Ist ri Aryani, I. A. Put ri Ut ami, I.B. Gaga Part am a, and D.A. Ast uti. 2009. The Effect of Ragi t ape ferment at ion product s in diet on nutrient s digest ibilit y and grow th perform ance of Bali drake. Proceeding. The 1 st Int ernat ional Seminar on Animal Indust ry 2009. Sust ainable Animal Product ion for Food Securit y and Safet y. 23-24 November 2009. Facult y of Animal Science, Bogor Agricult ural Universit y. Pp:180-187

J. Biol. Chem. Research. 49