Biol. Chem. Research. 49 Isolation of Saccharomyces Spp from Manure of Beef Bali Cattle as a Probiotics Properties and has CMC-ase Activity to Improve Nurient Quality of Rice Bran.

I solation of……………………………….Rice Bran Candraw ati, et al., 20 14 M any st udies have clearly demonst rat ed t hat t he addition of probiot ics culture or enzym es t o diet s rich in NSP result ed in a significant reduction of the int est inal viscosit y, enhances energy and prot ein utilization. Wang et al. 2004 report ed that degree of microbial ferm ent at ion in the large int est ine improves t he bioavailabilit y of diet ary. Yi et al. l996 report ed that supplem ent at ion of m icrobial in diet s improved N ret ent ion in broiler chickens and in vit ro digest ibilit y of veget able prot ein. Also, Chen et al. 2005 report ed t hat addit ion of 0.20 complex probiotic L. acidophilus and S. cerivisae in basal diet s increased digest iblit y of DM and CP. Hong et al. 2004 report ed t hat ferment at ion of feed using Aspergilus oryzae increased digest ibilit y of it s DM and CP. The inclusion of soluble diet ary fiber w heat bran increased daily NSP excret ed from feces Wang et al., 2004; Suprapt i et al., 2008, increased bot h of m et abolizable energy and crude prot ein cont ent s of palm kornel meal Jaelani et al., 2008. Chen et al. 2005 report ed that diet ary supplem ent at ion of complex probiot ic slightly improved digest ibilit y of nut rient s. CONCLUSION It w as concluded t hat t here are t wo isolate Sc.S-6 and S-7 w ere isolat ed from manure of beef Bali cat tle sam ples, bot h w ere t he pot ensial as a probiot ics sources and has CM C-ase akct ivit y and it s ut ilizat ion in the rice bran ferm ent at ion could increase nut rient com posit ion and digest ibilit y of rice bran. ACKNOW LEDGEM ENTS Authors are ent irely grat eful for the chem ical analyses of sample by st aff of Nut rit ion Laboratory, Fakult y of Anim al Husbandry, Udayana Universit y, Denpasar-Bali. The authors also w ant t o thank the Ditbinlit abmas, Dirjen Dikt i, Depdiknas in Jakart a for funding t his research. A part of the present st udy w as financially support ed by Ditbinlit abm as, Dirjen Dikt i, Depdiknas in Jakart a. REFERENCES Ahm ad, R. Z. 2005. Used of Kham ir Saccharomyces cerevisiae for feeding. W art azoa Vol. 15 1 : 49-55 Assocciat ion of Official Analyt ical Chem ist s. l994. Official M et hods of Analysis. 15 t h Edition. Associoation of Analyt ical Chemist s, Arlingt on, Virginia pp. 1230 Bach Knudsen, K. E. 2001. The nut ritional significance of “ diet ary fiber” analysis. Anim. Feed Sci. Tech . 90 : 3-20 Bedford, M .R. and H.L. Classen. 1992. Reduct ion int est inal viscosit y through m anipulation of diet ary rye and pentosanase concent rat ion is affect ed t hrough changes in the carbohydrat e com posit ion of t he int est inal equous phase and result in improved w heat s and food conversion efficiency of broiler chicks. J. Nut r. 122:560-569 Bidura, I.G.N.G., D. A. Warm adew i, D.P.M .A. Candraw at i, I. G. A. Ist ri Aryani, I. A. Put ri Ut ami, I.B. Gaga Part am a, and D.A. Ast uti. 2009. The Effect of Ragi t ape ferment at ion product s in diet on nutrient s digest ibilit y and grow th perform ance of Bali drake. Proceeding. The 1 st Int ernat ional Seminar on Animal Indust ry 2009. Sust ainable Animal Product ion for Food Securit y and Safet y. 23-24 November 2009. Facult y of Animal Science, Bogor Agricult ural Universit y. Pp:180-187

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Vol. 3 1, No. 1: 3 9-52 20 14 I solation of……………………………….Rice Bran Candraw ati, et al., 20 14 Bidura, I.G.N.G., D. P. M . A. Candraw at i, D. A. Warmadew i, I. P. Suyadnya, and I.A.S. Aryani. 2011. The increase of prot ein digest ibility and m et abolizable energy of rice bran by Saccharomyces cerevisiae ferm ent ation. The 3rd Int ernat ional Conference on Bioscience and Biot echnology. M aint aining W orld Prosperit y t rhough Biosciences, Biot echnology and Reveget at ion. 21-22 Sept ember 2011. Udayana Universit y, Denpasar Bali, Indonesia. Organized by Udayana Universit y in Cooperat ion w ith Yamaguchi Universit y. Udayana Universit y Press, Denpasar, Bali. Pp: 98-105 Bidura, I.G.N.G., IG. M ahardika, IP. Suyadnya, IBG. Part am a, IGL. Oka, and I.A.S. Aryani. 2012. The implem ent at ion of Saccharomyces spp.n-2 isolat e culture isolat ion from t radit ional yeast culture for im proving feed qualit y and performance of m ale Bali ducking. Agricult ural Science Research Journal. ISSN-L: 2026-6073 Sept em ber: Vol. 2 9: 486-492 Bidura I.G.N.G., N. L. G. Sum ardani, T. I. Put ri, and I. B. Gaga Part ama. 2008. The effect of ferment ed diet s on body w eight gains, carcass and abdominal fat in bali ducks. J. Indon. Trop. Agric . Vol 33 4: 274 – 281 Cao, B.H., X.P. Zhang, Y.M . Guo, Y. Karasaw a, and T. Kum ao. 2003. Effect s of diet ary cellulose on grow th, nit rogen utilizat ion, ret ent ion time of diet s in digest ive t ract and caecal microflora of chickens. Asian-Aust . J. Anim. Sci. Vol 16 6: 863-866 Chesson, A. 1994. Feed Enzym es. Anim. Feed Sci. Technol. 45: 65-79 Chen, Y. J., K. S. Son, B. J. M in, J. H. Cho, O. S. Kwon, and I. H. Kim . 2005. Effect s of Diet ary Probiot ic on Growt h Performance, Nut rient s Digest ibilit y, Blood Charact erist ics and Fecal Noxious Gas Cont ent in Grow ing Pigs. Asian-Aust . J. Anim. Sci. Vol. 18 10: 1464-1468 Cho, J. H., B. J. M in, Y. J. Chen, J. S. Yoo, Q. Wang, J. D. Kim, and I. H. Kim . 2007. Evaluat ion of FSP ferm ent ed soy prot ein t o replace soybean m eal in w eaned pigs: Growt h performance, blood urea nit rogen, and t ot al prot ein concent rat ions in serum and nut rient digest ibilit y. Asian-Aust . J. Anim. Sci. Vol. 20 12:1874-1879 Choct , M . 2002. Non-St arch polysaccharides: effect on nut rit ive value. In: Poult ry Feedst uffs: Supply, Composition and Nut rit ive Value, pp.221-236. Ed. J.M . M cNab and K.N. Boorman, CABI Publishing. Corzo, G. and S. E. Gilliland. 1999. Bile salt hydrolase act ivit y of t here st rain of Lact obacillus acidhophilus . J. Dairy Sci. 82: 472-479 Dubey R. C. 2006. A Text book of Biot echnology. Fout rh Revised Enlarged Edit ion. Ram Nagar, New Delhi: S. Chand Company LTD. Han, I. K., J. H. Lee, X. S. Piao, and D. Li. 2001. Feeding and managem ent syst em t o reduce environment al pollut ion in swine product ion. Asian-Aust . J. Anim. Sci. 14: 432- 444 Hidayat , M .N. 2010. htt p: lambungsat u.blogspot .com 2010 04 m ikroba-dalam -saluran- pencernaan-t ernak_22.ht ml Hong, K.J., C. H. Lee, and S. W.Kim . 2004. Aspergillus oryzae GB-107 ferment at ion im proves nut ritional qualit y of food soybeans and feed soybean m eal. J. M ed. Food . 7: 430

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