Fish Behaviour Study .1 Fish behaviour in relation to contrast colour net panel

control. The control was the frequency of fish passing through the panel without a net. In voluntary behaviour, the fish was less active than the conditioned behaviour. This probably because of none of stimulus is used to stimulate the fish of not avoiding to pass through the net panel. The use of forcing panel to minimize the swimming area of fish in area B was very helpful to makes the fish swim more active. w w w y ± S E Figure 15. Comparison among the fish passing through the net panel on conditioned behaviour From Figure 15 above, the comparison of fish passing through the net panel on conditioned behaviour was not significant. The fish becoming more active than if it was in voluntary because of the use of forcing panel. This made the frequency of passing in each of net panel of conditioned behaviour increasing, especially in both white nets old white and new white. The frequency of passing in both white nets i.e. the old white net and new white net was higher than the black net, with the highest frequency was in old white net. It showed that the fish was actually avoiding the contrast colour. 33

4.2.1.1 Net colour transparency

From the result of TWO-WAY ANOVA α= 0.05, the net colour transparency between old white, new white and black painted has significant difference. Table 5. Signification level of comparison proportion among three types of net panel Net colour Old white Black painted New white Old white 0.072 0.967 Black painted 0.049 New white Notes: significant in p0.05 Table 5 shown the signification level to compare the proportion among three types of net panel in voluntary behaviour. According of the signification level between the three netting panel, it showed that the comparison between the new white and black painted was the most significant.

4.2.1.2 Fish behaviour in relation to netting panel

The behaviour of fish recorded on video tape were 1 Japanese Jack mackerels tend to swim in circling movement around and near the mesh panel with their school; 2 After succeed passing the mesh and entering the other side of experimental tank, they tend to return to the previous side, and joined their school; 3 Japanese jack mackerel was able to see net barrier on their swimming movement, but intend to pass; 4 Japanese jack mackerels was avoiding the black panel.

4.2.2 Behaviour experiment using specific white net panels

From the result of the experiment, most of fish was to keep clear the mesh panel. According to Glass et al., 1993 keeping clear of mesh panel is considered as the natural behaviour of fish. That would be the explanation, why it was very difficult to stimulate fish to enter the mesh in voluntary. 34 As the result of the frame on frame analysis on mini DV, Japanese Jack mackerel did the reaction behaviour when they recognized the existence net panel on their swimming movement, stop first, and tried to pass the net panel, either way they avoiding the net panel and just swimming in circling. The Jack mackerels were not very active in voluntary unless they were given some stimulus, for example to herd their swimming movement, the treatment that was given as conditioned behaviour on this experiment. w w w w y y ± S E Figure 16. Comparison of fish passing through the net panel The proportion of the frequency of passing in Figure 16 derived from the frequency of fish passing through each net panel divided into frequency of fish passed through control. The control was the frequency of fish passing through the panel without a net. The result of the experiment showed that the frequency of passing among white nets is not significant; it means that there is no effect of transparency among three types of white nets. It makes that operating old white net or new white net considering similar in fish to pass. If this result will be implemented on the habit of fishermen, the use of old white net considering fine to the fishermen in order to minimize the cost, as long as the web is less of damages and still in good condition. 35 w w w w y Figure 17. Proportion of fish passing through the net Figure 17 showed that in 10 trials of this behavioural experiment, the frequency of fish passing was fluctuated. This was because of the natural behaviour of this fish that sometimes very active but sometimes inactive. It was very difficult to maintain the fish in stable condition in each trial, even though external factors that probably could influence the experimental result such as illumination, temperature, time of food giving are being controlled. From this result, it can be derived that animal including also fishes, have their inactive period and active period of swimming, in this case the swimming of passing through the net. This result only try to described the activity of fish during each trial, but from statistical test it can be inferred that if the net transparency of a net panel less visible, then the more fish passing through it.

4.2.2.1 Mesh passing reaction of fish

As the result of observation on the conditioned behaviour, the behavioural reactions of fish when passed the netting panel were classified into two types of behaviours, which are passed through netting panel with non-contact or contact. Contact behaviour is when the fishes passed through without any contact of their body, while non contact is the opposites way. 36 From the result of one way ANOVA on old white, new white and white dyed net panel showed that most fish could pass through the mesh without any contact. Most fishes always try to keep clear on netting panel then try to pass through the netting panel. It means that non contact behaviour is significant behaviour reaction in all of netting panel used on the experiment. Figure 18. Contact and non-contact reaction of fish passing through old white net panel Figure 18, 19, and 20 are proportion of fish passed through the netting panels old white, new white and white dyed. The value of proportion derives from the frequency of fish passed the net with or without contact divided total number of fish passed through the net. From the three panels used on the experiment, old white netting panel has the largest number proportion on non- contact reaction on passing. That is considering because most fish could be able to pass this net without any contact, if it compares within the others. Figure 19. showed that most of fish could be able to pass the old white net without any contact 12.032. It means that non-contact behaviour was dominant against contact behaviour. This happen because of the hanging ratio of the netting panel 0.65 and the mesh perimeter that used in the experiment was bigger than in the real fishing operation hanging ratio around 0.4-0.45. 37 Figure 19. Contact and non-contact reaction of fish passing through new white net panel Figure 19 above showed that most of fish could be able to pass the new white net without any contact 8.999. It means that non-contact behaviour was dominant against contact behaviour. This happen because of the hanging ratio of the netting panel 0.65 and the mesh perimeter that used in the experiment was bigger than in the real fishing operation hanging ratio around 0.4-0.45. Figure 20. Contact and non-contact reaction of fish passing through white dyed net panel 38 Figure 20 showed that most of fish could be able to pass through the white dyed netting panel without any contact 7.185. It means that non-contact behaviour was dominant against contact behaviour. This happen because of the hanging ratio of the netting panel 0.65 and the mesh perimeter that used in the experiment was bigger than in the real fishing operation hanging ratio around 0.4-0.45. The reason of using bigger hanging ratio in behaviour study is to avoid fishes got injured when they were passing the mesh, so the behaviour of passed could be observed. In actual fishing operation, the fish suppose to get caught by the net, and then the hanging ratio and mesh perimeter considered being smaller.

4.2.2.2 Behaviour of fish in relation to net colour

Proportion of fish passed through different colour of mesh panels is shown in Figure 21. As we could see from the figure, old white net has the highest proportion of fish passed through the mesh. Wardle et al. 1991 stated that the net colour appearance underwater was affected by many factors, at low intensities gillnets made by fine twines chosen to be relatively invisible to the fish. If the luminance of an object matches the luminance of the background then the object will see as invisible. w w w w y Figure 21. Proportion of fish passed through the net colour 39 With one way Anova, the significance of the influence of colour on the frequency of fish passed through the mesh showed no significance different. All of the net panels are considered to have capability of facilitating fish to pass and the old white net panel is the highest. This means that fish was avoiding the more visible white transparency. 4.3 Capture Process Experiment 4.3.1 Catch composition The catch composition of millennium gillnet dominated by threadfins Polynemus spp. for 71.287 of catch. While by-catch were consists of striped catfish eel Plotosus lineatus, spotted catfish Arius maculatus, great barracuda Sphyraena barracuda, triple tail Labotes surinamensis, barramundi Lates calcarifer, blue spotted snapper Lutjanus rivulatus, silver pomfret Pampus argentus, stingray Dasyatis spp., garfish Hemirhampus spp. and shark Charcarinus spp.. Table 6. Species composition in percentage and total length TL of fish caught by millennium gillnet No English name Scientific name TL cm Number of catch Catch 1 Threadfins Polynemus spp. 34-73 72 71.287 2 Striped catfish eel Plotosus lineatus 32-82 11 10.891 3 Spotted catfish Arius maculatus 44-53 7 6.931 4 Great barracuda Sphyraena barracuda 89-94 2 1.980 5 Triple tail Labotes surinamensis 45-57 2 1.980 6 Barramundi Lates calcarifer 63-72 2 1.980 7 Blue spotted snapper Lutjanus rivulatus 30 1 0.990 8 Silver pomfret Pampus argentus 30 1 0.990 9 Rayfish Dasyatis spp. 180 1 0.990 10 Garfish Hemirhampus spp. 78 1 0.990 11 Shark Charcarinus spp. 58 1 0.990 Total 101 100 Note: demersal fish From the Table 6, the catch of millennium gillnet came from various species, but it dominated by threadfins. Most of the catches also were demersal species, even though actually the target catch of millennium gillnet was pelagic fishes. 40 This was because fishermen operated the millennium gillnet in the shallow waters of Bondet Waters around 7-10 m of depth and the distance of fishing operation just around 0-12 miles from the coastal zone. Since the body net was around 7.8 m of depth then the demersal species were caught by this gillnet. If this gillnet were operated in deeper and further waters area, the catch were dominates by pelagic species, such as barred Spanish mackerel, frigate tuna, sailing fish, etc as seen in Karangsong, Indramayu. In Indramayu, millennium gillnet is operated in industrial scale, not the artisanal fishery. They using larger fishing boat around 20 GT and operates in the ocean and waters more than 12 miles. The period of fishing operation is also longer than in that of Bondet Waters. Bondet fishermen operates gillnet in one day fishing, while Indramayu fishermen operates this net until a month of fishing.

4.3.2 Capture conditions

Figure 22 and Figure 23 shown that the threadfins were mostly caught in the millennium gillnet by gilled. The class range divided into 6 classes, the largest frequency is on 48-54.9 cm with 24 fish. S W Figure 22. Captured condition of threadfins on class range cm Figure 22 shown the captured condition of threadfins as the dominant catch of the millennium gillnet. From this figure, most of threadfins concentrated on the class range of total length around 48 to 54.9 cm. This means that most of threadfins that caught by this gillnet was in the medium size. 41