3.5.2 Analysis of height of plants from wild-type and various transgenic lines
Figure 43 Analysis of height of plants from wild-type and various transgenic lines. A
Evolution of plants height for 12-month-old B Height of 12-month-old plants. The data were analysed with XLSTAT software. Statistical analysis was
performed with an ANOVA followed by the Tukey test. Values with the same letter were not significantly different at the 0.05 probability level
Analysis on Figure 43A showed five transgenic lines had a higher height compared to wild-type. They were TS18A37, TS19A46, TS20A47, TS20A69, and TS20A75. Based on
statistical analysis Figure 43B showed there were two transgenic lines TS19A46 and TS20A69 had significantly differences compared to wild-type. Figure 44 showed the
transgenic lines could grow 1.5-2 times higher than wild-type.
Figure 44 Twelve-month-old plants
Analysis of plant height showed a significant difference in 0 month of plant before acclimatization between plants from constructs HEV2.1::HbERF-IXc5 and wild-type. On the
other hand, there is no significantly different in month 2, 6, and 12 of plant growth Table 24.
Table 24 Effect of constructs on plant height
Construct Month after acclimatization
2 6
12
Wild-type 3.747
a
8.656
ab
19.789
a
54.547
ab
HEV2.1::HbERF-IXc4 5.044
ab
7.797
a
20.172
a
47.236
a
35S::HbERF-IXc5 4.591
ab
7.977
a
23.517
a
58.767
ab
HEV2.1::HbERF-IXc5 5.821
b
10.239
b
26.695
a
69.859
b
3.5.3 Analysis of stem diameter of plants from wild-type and various transgenic lines
Figure 45 Analysis of stem diameter of plants from wild-type and various transgenic lines.
A Evolution of plant stem diameter for 12-month-old B Stem diameter of 12- month-old plants. The data were analysed with XLSTAT software. Statistical
analysis was performed with an ANOVA followed by the Tukey test. Values with the same letter were not significantly different at the 0.05 probability level
The mean of stem diameter values Figure 45A showed some transgenic lines TS18A37, TS18A13, TS18A69, TS20A47, TS20A69, and TS20A75 had a greater stem
diameter than wild-type. Statistical analysis Figure 45B showed no significant difference between wild-type and transgenic lines except the construct of 35S::HbERF-IXc5
TS19A46. TS19A46 had highest average value of stem diameter of 12-month-old plants and more vigorous compared the others.
Based on statistical analysis, there is significantly different in diameter of plants between constructs HEV2.1::HbERF-IXc4 and 35S::HbERF-IXc5 compared to wild-type at 2
months of plant growth. There is no significant difference in plant growth for 0, 6, and 12 months after acclimatization between wild-type and the various tested constructs Table 25.
Table 25 Effect of constructs on plant stem
Construct Month after acclimatization
2 6
12
Wild-type 0.124
a
0.213
b
0.323
a
0.657
a
HEV2.1::HbERF-IXc4 0.132
a
0.179
a
0.314
a
0.715
a
35S::HbERF-IXc5 0.141
a
0.172
a
0.323
a
0.775
a
HEV2.1::HbERF-IXc5 0.147
a
0.190
ab
0.352
a
0.847
a
3.5.4 Analysis of leaves and leaflets of plants from wild-type and various transgenic lines
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