Table 25 Effect of constructs on plant stem
Construct Month after acclimatization
2 6
12
Wild-type 0.124
a
0.213
b
0.323
a
0.657
a
HEV2.1::HbERF-IXc4 0.132
a
0.179
a
0.314
a
0.715
a
35S::HbERF-IXc5 0.141
a
0.172
a
0.323
a
0.775
a
HEV2.1::HbERF-IXc5 0.147
a
0.190
ab
0.352
a
0.847
a
3.5.4 Analysis of leaves and leaflets of plants from wild-type and various transgenic lines
Figure 46 Analysis of leaves of plants from wild-type and various transgenic lines. A
Evolution of number of plants leaves for 12-month-old B Number of leaves of 12-month-old plants. The data were analysed with XLSTAT software. Statistical
analysis was performed with an ANOVA followed by the Tukey test. Values with the same letter were not significantly different at the 0.05 probability level
Figure 47 Analysis of leaflets of plants from wild-type and various transgenic lines. A
Evolution of number of plants leaflets for 12-month-old B Number of leaflets of 12-month-old plants. The data were analysed with XLSTAT software. Statistical
analysis was performed with an ANOVA followed by the Tukey test. Values with the same letter were not significantly different at the 0.05 probability level
Number of leaves and leaflets from wild-type and transgenic lines tend to increase until the plants are 12-month-old, except TS17A61 which decreased the number of leaves and
leaflets in the 2
nd
month. Based on statistical analysis of leaves and leaflets, TS20A47 had the highest number of leaves and leaflets compared to wild-type Figure 46 47. It was related
with the lack of number of plants from this line.
Table 26 Effect of constructs on number of leaves
Construct Month after acclimatization
2 6
12
Wild-type 1.328
a
2.406
a
4.750
a
7.117
a
HEV2.1::HbERF-IXc4 1.939
b
2.404
a
6.425
a
8.847
a
35S::HbERF-IXc5 1.356
a
2.074
a
6.048
a
8.028
a
HEV2.1::HbERF-IXc5 1.590
a
2.385
a
6.741
a
10.229
a
Table 27 Effect of constructs on number of leaflets
Construct Month after acclimatization
2 6
12
Wild-type 3.489
a
6.739
a
12.989
a
12.050
a
HEV2.1::HbERF-IXc4 4.923
b
6.608
a
18.606
a
25.126
a
35S::HbERF-IXc5 3.428
a
5.574
a
17.798
a
23.186
a
HEV2.1::HbERF-IXc5 3.993
a
6.521
a
19.584
a
29.216
a
The statistical analysis showed there is no significant difference in the number of leaves and leaflets during the culture but in 0 month. There is a significant difference between
construct HEV2.1::HbERF-IXc4 and wild-type for 0 month Table 26 and 27
3.5.5 Analysis of weight of 12-month-old plants from wild-type and various transgenic lines
