Uji lanjut Duncan a.
Entodinium 24 jam b. Diplodinium 24 jam
c.Dasytricha 24 jam d. Total protozoa 24 jam
5.5. Populasi protozoa pada 48 jam inkubasi
Sumber Parameter
Jumlah kuadrat db
Kuadrat tengah
F Sig.
Model terkoreksi Entodinium48
7.632a 4
1.908 510.939
.000 Diplodinium48
3.546b 4
.887 41.303
.000 Dasytricha48
.000c 4
.000 .
. TotalProtozoa48
9.015d 4
2.254 1106.563
.000 Intersep
Entodinium48 309.110
1 309.110
82771.155 .000
Diplodinium48 249.020
1 249.020
11600.453 .000
Dasytricha48 145.692
1 145.692
. .
TotalProtozoa48 326.416
1 326.416
160264.397 .000
Perlakuan Entodinium48
7.632 4
1.908 510.939
.000 Diplodinium48
3.546 4
.887 41.303
.000 Dasytricha48
.000 4
.000 .
. TotalProtozoa48
9.015 4
2.254 1106.563
.000 Galat
Entodinium48 .056
15 .004
Diplodinium48 .322
15 .021
Dasytricha48 .000
15 .000
TotalProtozoa48 .031
15 .002
Total Entodinium48
316.799 20
Diplodinium48 252.889
20 Dasytricha48
145.692 20
TotalProtozoa48 335.461
20 Total terkoreksi
Entodinium48 7.688
19 Diplodinium48
3.868 19
Dasytricha48 .000
19 TotalProtozoa48
9.046 19
Perlakuan N
Subset, alfa = 0.01 1
2 5.00
4 2.6990
4.00 4
4.7570 1.00
4 4.7635
3.00 4
4.8028 2.00
4 4.8358
Sig. 1.000
.034 Perlakuan
N Subset, alfa=0.01
1 2
5.00 4
2.6990 4.00
4 3.9575
1.00 4
3.9585 2.00
4 3.9740
3.00 4
4.0003 Sig.
1.000 .667
Perlakuan N
Subset, alfa=0.01 1
2 5.00
4 2.6990
3.00 4
3.4255 1.00
4 3.5435
2.00 4
3.5875 4.00
4 3.5875
Sig. 1.000
.311 Perlakuan
N Subset, alfa=0.01
1 2
5.00 4
2.6990 4.00
4 4.8483
1.00 4
4.8515 3.00
4 4.8913
2.00 4
4.9148 Sig.
1.000 .031
Uji lanjut Duncan a. Entodinium 48 jam
b. Diplodinium 48 jam
c.Total protozoa 48 jam
Treatment N
Subset, alfa=0.01 1
2 5.00
4 2.6990
4.00 4
4.3090 1.00
4 4.3810
2.00 4
4.4010 3.00
4 4.4095
Sig. 1.000
.010 Perlakuan
N Subset, alfa=0.01
1 2
5.00 4
2.6990 2.00
4 3.6680
4.00 4
3.6838 1.00
4 3.7195
3.00 4
3.8728 Sig.
1.000 .087
Perlakuan N
Subset, alfa=0.01 1
2 3
5.00 4
2.6990 4.00
4 4.1623
3.00 4
4.2415 4.2415
1.00 4
4.2550 4.2550
2.00 4
4.2990 Sig.
1.000 .059
.226
Lampiran 6. Analisis statistik data percobaan in vitro tahap II.
6.1. Populasi protozoa inkubasi 4 dan 24 jam
Sumber Parameter
Type III Sum of Squares
db Jumlah
kuadrat F
Sig. Model terkoreksi
Protozoa 4 jam 8.287a
10 .829
1.164 .379
Protozoa 24 jam 20.082b
10 2.008
3.520 .012
Intersep Protozoa 4 jam
414.411 1
414.411 582.199
.000 Protozoa 24 jam
365.098 1
365.098 639.887
.000 Substrat
Protozoa 4 jam 1.416
2 .708
.994 .392
Protozoa 24 jam 3.793
2 1.897
3.324 .062
Lerak Protozoa 4 jam
2.444 2
1.222 1.717
.211 Protozoa 24 jam
7.136 2
3.568 6.254
.010 Kelompok
Protozoa 4 jam 3.291
2 1.645
2.312 .131
Protozoa 24 jam 5.411
2 2.705
4.742 .024
Substrat Lerak Protozoa 4 jam
1.137 4
.284 .399
.806 Protozoa 24 jam
3.741 4
.935 1.639
.213 Galat
Protozoa 4 jam 11.389
16 .712
Protozoa 24 jam 9.129
16 .571
Total Protozoa 4 jam
434.087 27
Protozoa 24 jam 394.309
27 Total terkoreksi
Protozoa 4 jam 19.676
26 Protozoa 24 jam
29.211 26
Uji lanjut Duncan
a.
Protozoa 24 jam
Keterangan : Substrat : 1 = H:K=90:10
Lerak : 1 = 0 mgml ekstrak lerak 2 = H:K=80:20
2 = 0.6 mgml ekstrak lerak 3 = H:K=70:30
3 = 0.8 mgml ekstrak lerak Substrat
N Subset, alfa=0.01
1 2
2.00 9
3.2912 1.00
9 3.5557
3.5557 3.00
9 4.1848
Sig. .468
.096 Lerak
N Subset, alfa=0.01
1 2
3.00 9
3.2163 2.00
9 3.4208
1.00 9
4.3947 Sig.
.574 1.000
6.2. Populasi Total bakteri, R. albus, F. succinogenes dan Prevotella sp.
Sumber Parameter
Jumlah kuadrat db
Kuadrat tengah F
Sig. Model terkoreksi
TB .247a
10 .025
1.796 .148
FS 1126.230b
10 112.623
4.267 .006
RA 173.576c
10 17.358
1.131 .402
Prevotela 296.363d
10 29.636
1.700 .171
Intersep TB
1927.287 1
1927.287 140221.120
.000 FS
1547.043 1
1547.043 58.610
.000 RA
548.728 1
548.728 35.761
.000 Prevotela
479.151 1
479.151 27.479
.000 Substrat
TB .077
2 .038
2.790 .093
FS 294.248
2 147.124
5.574 .015
RA 1.908
2 .954
.062 .940
Prevotela 11.797
2 5.898
.338 .718
Lerak TB
.059 2
.029 2.130
.153 FS
2.769 2
1.384 .052
.949 RA
92.640 2
46.320 3.019
.079 Prevotela
120.226 2
60.113 3.447
.059 Kelompok
TB .052
2 .026
1.907 .183
FS 821.188
2 410.594
15.556 .000
RA 70.149
2 35.075
2.286 .136
Prevotela 121.250
2 60.625
3.477 .057
Substrat Lerak TB
.065 4
.016 1.190
.355 FS
11.797 4
2.949 .112
.976 RA
17.286 4
4.322 .282
.885 Prevotela
34.265 4
8.566 .491
.742 Galat
TB .206
15 .014
FS 395.930
15 26.395
RA 230.167
15 15.344
Prevotela 261.556
15 17.437
Total TB
1970.133 26
FS 2967.014
26 RA
931.684 26
Prevotela 1008.281
26 Total terkoreksi
TB .453
25 FS
1522.160 25
RA 403.743
25 Prevotela
557.919 25
Uji lanjut Duncan a. Prevotela
6.3. Aktivitas enzim amylase, CMCase dan xylanase pada inkubasi 4 jam
Sumber Parameter
Jumlah kuadrat db
Kuadrat tengah
F Sig.
Model terkoreksi Amilase4j
32.794a 10
3.279 2.323
.064 CMCase4j
17.041b 10
1.704 3.543
.012 Xylanase4j
104.422c 10
10.442 2.971
.026 Intersep
Amilase4j 2616.986
1 2616.986
1853.943 .000
CMCase4j 698.607
1 698.607
1452.569 .000
Xylanase4j 4337.197
1 4337.197
1233.965 .000
Substrat Amilase4j
3.511 2
1.756 1.244
.315 CMCase4j
.293 2
.147 .305
.741 Xylanase4j
5.857 2
2.929 .833
.453 Lerak
Amilase4j 14.027
2 7.014
4.969 .021
CMCase4j 2.114
2 1.057
2.198 .143
Xylanase4j 36.555
2 18.278
5.200 .018
Substrat Lerak Amilase4j
8.456 4
2.114 1.498
.250 CMCase4j
1.520 4
.380 .790
.548 Xylanase4j
27.305 4
6.826 1.942
.152 Kelompok
Amilase4j 6.799
2 3.400
2.408 .122
CMCase4j 13.113
2 6.557
13.633 .000
Xylanase4j 34.704
2 17.352
4.937 .021
Galat Amilase4j
22.585 16
1.412 CMCase4j
7.695 16
.481 Xylanase4j
56.238 16
3.515 Total
Amilase4j 2672.365
27 CMCase4j
723.343 27
Xylanase4j 4497.856
27 Total terkoreksi
Amilase4j 55.379
26 CMCase4j
24.736 26
Xylanase4j 160.659
26 Substrat
N Subset, alfa=0.05
1 1.00
8 3.3038
3.00 9
3.8033 2.00
9 5.2833
Sig. .365
Lerak N
Subset, alfa=0.05 1
2 1.00
9 2.3200
2.00 9
3.2511 3.2511
3.00 8
7.2588 Sig.
.650 .065
Uji lanjut Duncan
a. Aktivitas amylase 4 jam inkubasi
