Efek Ekstrak Etanol Daun Sirih Merah (EEDSM) Terhadap Penurunan Kadar Gula Darah Serta Gambaran Histologi Pankreas Mencit (Mus Musculus L) Diabetes
61 LAMPIRAN Lampiran 1. Tumbuhan sirih merah (Piper crocatum)
62 Lampiran 2. Ethical Clearence hewan percobaan dari Komite Etik Lampiran 3. Surat Keterangan Penelitian di Laboratorium Fitokimia Fak. Farmasi USU
63 Lampiran 4. Surat Keterangan Penelitian di Laboratorium Farmakologi Fak. Farmasi USU
64
Lampiran 5. Data kadar gula darah toleransi yang diinduksi d-glukosa
Perlakuan KGD (mg/dL) awal menit ke-30 menit ke-60 menit ke-90 menit ke-120
CMC 1 94 137 142 143 127 2 84 183 117 114 104 3 96 253 259 151 145
Purata 91,3 191,0 172,7 136,0 125,3 Sd 6,4 58,4 75,8 19,5 20,6 Metformin 1 86 191 124
77
82 2 93 293 97 94 117 3 85 219 145
91
89 Purata 88,0 234,3 122,0 87,3 96,0 Sd 4,4 52,7 24,1 9,1 18,5 EEDSM 10 1 87 308 154 124 145
2 98 246 152 127 133 3 76 246 171 148 245 Purata 87,0 266,7 159,0 133,0 174,3 Sd 11,0 35,8 10,4 13,1 61,5 EEDSM 100 1 87 212 145 108 103
2 87 302 210 117 105 3 92 155 182
92
71 Purata 88,7 223,0 179,0 105,7 93,0 Sd 2,9 74,1 32,6 12,7 19,1 EEDSM 1000 1 93 365 180 135 176
2 102 222 148 127 139 3 87 372 263 221 171 Purata 94,0 319,7 197,0 161,0 162,0 Sd 7,5 84,7 59,4 52,1 20,1
Lampiran 6. Profil kadar gula darah mencit diabetes yang diberi ekstrak sirih merah
Jenis Perlakuan BB Kadar Gula Darah (mg/dL) 0 hari 1 hari 3 hari 5 hari 7 hari 9 hari
11 hari base line 1 38,4
95 97 100
96 94 102
95
2 30 121 120 115 118 125 130 120 3 33,3 116 118 120 115 117 121 116 4 33,7 105 110 115 101 115 120 100 5 35,4 114 105 109 118 110 102 115 6 34,1 108 115 120 117 101 106 110 purata 109,8 110,8 113,2 110,8 110,3 113,5 109,3 sd 9,2 8,7 7,6 9,7 11,3 11,8 9,8
Kontrol negatif, CMC 0,5%
1
39 91 478 587 566 597 mati 2 40,8 95 568 599 mati 3 35,2 101 508 530 528 540 537 550
4 37,8 105 549 568 570 589 mati 5 36,6 98 487 500 510 515 525 535 6 35,5 100 490 505 515 520 530 540 purata 98,3 513,3 548,2 537,8 552,2 530,7 541,7 sd 4,9 36,8 42,4 28,4 38,5 6,0 7,6
EEDSM 50 mg/kg 1 39,4 102 526 543 488 425 378 382 2 34,5
87 563 463 376 384 363 341 3 35,7 96 598 436 441 375 303 275 4 34,8 88 498 476 398 357 346 280 5 33,5 105 478 465 445 405 350 327 Jenis Perlakuan BB Kadar Gula Darah (mg/dL) 0 hari 1 hari 3 hari 5 hari 7 hari 9 hari
11 hari 6 37,3 115 503 518 480 453 405 315 purata 98,8 527,7 483,5 438,0 399,8 357,5 320,0 sd 10,7 45,1 39,5 44,2 35,2 34,3 40,0
EEDSM 100 mg/kg 1 35,7 91 542 513 488 397 331 255 2 34,2
87 422 386 356 304 263 181 3 33,3 103 488 453 393 337 285 228 4 32,7 114 392 388 375 324 275 217 5 31,9
99 479 452 397 335 284 198 6 33,7 106 501 529 427 352 257 184 purata 100,0 470,7 453,5 406,0 341,5 282,5 210,5 sd 9,9 54,7 60,1 46,6 31,5 26,3 28,5
EEDSM 200 mg/kg 1 28,5 108 470 403 347 283 245 196
2
31 69 500 401 379 335 297 161 3 39,2 107 396 303 278 213 166 96 4 35,5
79 482 392 351 321 275 168 5 35,1 97 512 409 374 292 185 128 6 34,6 105 537 459 358 284 194 125 purata 94,2 482,8 394,5 347,8 288,0 227,0 145,7 sd 16,4 48,5 50,7 36,5 42,4 53,1 36,0
Metformin 10 mg/kg BB 1 38,5 86 526 392 288 204 152 102
2
31 92 587 436 312 356 297 238 3 39,2 106 477 420 293 187 159 123 4 35,5 89 428 317 285 165 149 128 5 32,4 116 579 538 453 341 289 253
Kadar Gula Darah (mg/dL) Jenis Perlakuan BB
11 0 hari 1 hari 3 hari 5 hari 7 hari 9 hari hari 6 31,9
98 514 536 416 327 268 187 purata 97,8 518,5 439,8 341,2 263,3 219,0 171,8 sd 11,4 60,6 85,6 73,8 86,8 72,6 63,8
Lampiran 7. Berat pankreas mencit diabetes yang diberi ekstrak etanol sirih merah dengan kontrol positif metformin
Jenis Perlakuan N Pankreas (mg) 1 141 2 128 3 163
Base Line 4 151 5 150 6 143
Purata 146,0 Sd 11,7 1 156
2 124 3 138 Kontrol Negatif 4 149
5 152 6 125 Purata 140,7 Sd 13,9 1 180
2 160 3 155 EEDSM 50 mg/kg BB 4 166
5 175 6 157 Purata 165,5 Jenis Perlakuan N Pankreas (mg) Sd 10,1 1 165
2 219 3 217 EEDSM 100 mg/kg BB 4 141
5 179 6 182 Purata 183,8 Sd 30,2 1 195
2 254 3 210 EEDSM 200 mg/kg BB 4 169
5 205 6 203 Purata 206,0 Sd 27,6 1 147
2 132 3 128 metformin 10 mg/kg BB 4 155
5 141 6 139 Purata 140,3 Sd 9,8 Jenis Perlakuan N Pankreas (mg) 1 156 2 127 3 189
EEDSM 100 mg/kg BB, preventif 4 198 5 163 6 154
Purata 164,5 Sd 25,7
Lampiran 8. Data berat badan mencit diabetes yang diberi ekstrak etanol sirih merah dengan kontrol positif metformin
Jenis Perlakuan Berat Badan (g)
BB awal 1 hari
3 hari 5 hari
7 hari 9 hari
11 hari Base Line 1 31,1 31,7 33,0 33,7 34,3 36,5 38,4
2 28,4 29,0 30,1 30,7 31,3 28,5
30 3 29,1 29,7 30,9 31,5 32,1 31,6 33,3 4 28,6 29,2 30,3 30,9 31,6 32,0 33,7 5 28,7 29,2 30,4 31,0 31,6 33,6 35,4 6 27,6 28,2 29,3 29,9 30,5 32,4 34,1
Purata 28,9 29,5 30,7 31,3 31,9 32,4 34,2 Sd 1,2 1,2 1,2 1,3 1,3 2,6 2,7 Kontrol Negatif 1 39 35,3 28,7 24,6 25,1 mati
2 40,8 37,3 29,5 mati 3 35,2 32,4 31,7 30,2 29,5 28,3 28,5 4 37,8 35,7 31,6 28,7 26,4 mati 5 36,6 34,7 32,9 31,3 29,5 27,1 26,7 6 35,5 32,7 31,2 28,4 27,1 26,7 25,4
Purata 37,5 34,7 30,9 28,6 27,5 27,4 26,9 Sd 2,2 1,9 1,6 2,5 1,9 0,8 1,6 EEDSM 50 mg/kg BB 1 39,4 36,9 34,4 33,6 34,2 37,6 36,3
2 34,5 32 29,6 32,7 33,6 30,8 31,5 3 35,7 33,5 33,4 31,8 31,2 29,3 30,2 4 34,8 35,4 31,7 30,6 30,3 29,4 30,9 5 33,5 32,3 31,4 30,3
30 29,8 30,2 Jenis Perlakuan Berat Badan (g)
BB awal 1 hari
3 hari 5 hari
7 hari 9 hari
11 hari 6 37,3 36,5 35,6 34,1 32,7 31,8 30,2
Purata 35,9 34,4 32,7 32,2 32,0 31,5 31,6 Sd 2,2 2,1 2,2 1,6 1,8 3,2 2,4 EEDSM 100 mg/kg BB 1 35,7 34,3 25,1 29,7 33,5 29,8 28,9
2 34,2 27,5 26 30,4 35,9 33,1
34 3 33,3 26,7 29,2 30,2 30,8 29,5 30,3 4 32,7 31 35,5 35 35,8 36,1 36,6 5 31,9 30,1 31,4 32,6
32 32,7 33,3 6 33,7 31,7 32,6 33 34,1 35 35,2
Purata 33,6 30,2 30,0 31,8 33,7 32,7 33,1 Sd 1,3 2,8 4,0 2,1 2,0 2,7 2,9 EEDSM 200 mg/kg BB 1 28,5 24,5 28,7 30,2 29,6 28,3
29 2 31 26,8 32,8 33,5 31,4 29,7 31,3 3 39,2 33,6 26,7 32,1 36,9 35,8
37 4 35,5 31,6 30,1 32,7 34,8 37,9 40,7 5 35,1 32,4 30,5 33,6 36,2 38,6 41,5 6 34,6 33,4 31,7 34,9 36,8
38 39,7 Purata 34,0 30,4 30,1 32,8 34,3 34,7 36,5 Sd 3,7 3,8 2,2 1,6 3,1 4,5 5,2 metformin 10 mg/kg BB
1 38,5 34 33,1 31,7 31,1 30,7
30 2 31 24,3 26,5 28,2 29,3 28 28,7 3 39,2 29,7 31,3 29,7 31,5 33,4 34,5
4 35,5 31 30,6 31,8 32,4 31 32,8 5 32,4 32 31,8 31,2 29,4 30 30,8
Berat Badan (g) Jenis Perlakuan
BB
1
3
5
7
9
11 awal hari hari hari hari hari hari 6 31,9 31 30,4 30,9 31,3 29,1 29,5
Purata 34,8 30,3 30,6 30,6 30,8 30,4 31,1 Sd 3,5 3,3 2,2 1,4 1,2 1,8 2,2 1 29,2 33,2
2 28
22 3 27,7 31,8 4 28,9 37,6 5 26,5
30 EEDSM 100 mg/kg BB, preventif 6 33,2 33,6 Purata 28,9 31,4 Sd 2,3 5,2
Lampiran 9. Perubahan berat badan mencit diabetes selama sebelas hari
Perlakuan BB awal (g)
BB hari ke- 11 (g) Perubahan BB selisih % base line 1 31,1 38,4 7,3 23,5
2 28,4 30 1,6 5,6 3 29,1 33,3 4,2 14,4 4 28,6 33,7 5,1 17,8 5 28,7 35,4 6,7 23,5 6 27,6 34,1 6,5 23,5
Purata 28,9 34,2 5,2 18,0 Sd 1,2 2,7 2,1 7,1 kontrol negatif
1 39 0,0 2 40,8 0,0 3 35,2 28,5 -6,7 -19,0 4 37,8 0,0 5 36,6 26,7 -9,9 -27,0 6 35,5 25,4 -10,1 -28,5
Purata 37,5 26,9 -8,9 -12,4 Sd 2,2 1,6 1,9 14,0 EEDSM 50 mg/kg BB 1 39,4 36,3 -3,1 -7,9
2 34,5 31,5 -3,0 -8,7 3 35,7 30,2 -5,5 -15,4 4 34,8 30,9 -3,9 -11,2 5 33,5 30,2 -3,3 -9,9 6 37,3 30,2 -7,1 -19,0 Perlakuan BB awal (g)
BB hari ke- 11 (g) Perubahan BB selisih %
Purata 35,9 31,6 -4,3 -12,0 Sd 2,2 2,4 1,6 4,3 EEDSM 100 mg/kg BB 1 35,7 28,9 -6,8 -19,0
2 34,2 34 -0,2 -0,6 3 33,3 30,3 -3,0 -9,0 4 32,7 36,6 3,9 11,9 5 31,9 33,3 1,4 4,4 6 33,7 35,2 1,5 4,5
Purata 33,6 33,1 -0,5 -1,3 Sd 1,3 2,9 3,8 11,1 EEDSM 200 mg/kg BB 1 28,5
29 0,5 1,8 2 31 31,3 0,3 1,0 3 39,2 37 -2,2 -5,6 4 35,5 40,7 5,2 14,6 5 35,1 41,5 6,4 18,2 6 34,6 39,7 5,1 14,7
Purata 34,0 36,5 2,6 7,5 Sd 3,7 5,2 3,5 9,7 metformin 10 mg/kg BB
1 38,5 30 -8,5 -22,1 2 31 28,7 -2,3 -7,4 3 39,2 34,5 -4,7 -12,0 4 35,5 32,8 -2,7 -7,6 5 32,4 30,8 -1,6 -4,9 Perlakuan BB awal (g)
BB hari ke- 11 (g) Perubahan BB selisih %
6 31,9 29,5 -2,4 -7,5 Purata 34,8 31,1 -3,7 -10,3 Sd 3,5 2,2 2,6 6,2 EEDSM 100 mg/kg BB, preventif 1 29,2 33,2 4,0 13,7
2 28 22 -6,0 -21,4 3 27,7 31,8 4,1 14,8 4 28,9 37,6 8,7 30,1 5 26,5
30 3,5 13,2 6 33,2 33,6 0,4 1,2 Purata 28,9 31,4 2,5 8,6 Sd 2,3 5,2 4,9 17,3 Lampiran 10. Analisis data statistik kadar gula darah toleransi yang diinduksi d- glukosa Analisis normalitas data dengan metode uji Kolmogorov-Smirnov One-Sample Kolmogorov-Smirnov Test
awal menit_ ke_30 menit_ke_
Most Extreme Differences Absolute ,200 ,132 ,199 ,157 ,168 Positive ,200 ,132 ,199 ,157 ,168 Negative -,119 -,087 -,108 -,100 -,090
Within Groups 40260,000 10 4026,000 Total 68878,933
14 menit_ke_30 Between Groups 28618,933 4 7154,733 1,777 ,210
Awal Between Groups 97,067 4 24,267 ,492 ,742 Within Groups 493,333 10 49,333 Total 590,400
Analisis data dengan menggunakan Anova satu arah ANOVA Sum of Squares df Mean Square F Sig.
b. Calculated from data.
Kolmogorov-Smirnov Z ,775 ,512 ,772 ,606 ,651 Asymp. Sig. (2-tailed) ,585 ,956 ,591 ,856 ,791 a. Test distribution is Normal.
Mean 89,80 246,93 165,93 124,60 130,13 Std. Deviation 6,494 70,142 47,448 34,678 44,107
60 menit_ke_ 90 menit_ke_12
a,b
15 Normal Parameters
15
15
15
15
N
14 menit_ke_60 Between Groups 9478,267 4 2369,567 1,075 ,419 Within Groups 22040,667 10 2204,067 Total 31518,933
14 menit_ke_90 Between Groups 9818,267 4 2454,567 3,498 ,049
Within Groups 7017,333 10 701,733 Total 16835,600
14 menit_ke_120 Between Groups 16608,400 4 4152,100 3,907 ,037
Within Groups 10627,333 10 1062,733 Total 27235,733
14 Uji lanjutan rerata dengan menggunakan uji Duncan
awal
Duncan
a
Perlakuan N Subset for alpha = 0.2
1 EEDSM 10 mg/kg BB 3 87,00 Metformin 10 mg/kg BB 3 88,00 EEDSM 100 mg/kg BB 3 88,67 CMC 0,5% 3 91,33 EEDSM 1000 mg/kg BB 3 94,00 Sig. ,286 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
menit_ke_30
Duncan
a
perlakuan N Subset for alpha = 0.2
1
2 CMC 0,5% 3 191,00 EEDSM 100 mg/kg BB 3 223,00 Metformin 10 mg/kg BB 3 234,33 EEDSM 10 mg/kg BB 3 266,67 266,67 EEDSM 1000 mg/kg BB 3 319,67 Sig. ,203 ,330 Means for groups in homogeneous subsets are displayed.
Uses Harmonic Mean Sample Size = 3,000.
menit_ke_60
Duncan
a
perlakuan N Subset for alpha = 0.2
1
2 Metformin 10 mg/kg BB 3 122,00 EEDSM 10 mg/kg BB 3 159,00 159,00 CMC 0,5% 3 172,67 172,67 EEDSM 100 mg/kg BB 3 179,00 EEDSM 1000 mg/kg BB 3 197,00 Sig. ,236 ,376 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
menit_ke_90 a
Duncan Subset for alpha = 0.2 perlakuan N
1
2
3 Metformin 10 mg/kg BB 3 87,33 EEDSM 100 mg/kg BB 3 105,67 105,67 EEDSM 10 mg/kg BB 3 133,00 133,00 CMC 0,5% 3 136,00 136,00 EEDSM 1000 mg/kg BB 3 161,00 Sig. ,416 ,210 ,245 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Lampiran 11. Analisis data statistik kadar gula darah mencit diabetes yang diinduksi aloksan Analisis normalitas data dengan metode uji Kolmogorov-Smirnov One-Sample Kolmogorov-Smirnov Test
1 N
Kolmogorov-Smirnov Z 1,121 ,856 ,608 1,280 Asymp. Sig. (2-tailed) ,162 ,456 ,854 ,076
Most Extreme Differences Absolute ,175 ,134 ,097 ,205 Positive ,144 ,097 ,090 ,205 Negative -,175 -,134 -,097 -,153
3 148,2000 119,14002
Mean 306,1463 272,6585 225,359 217,1538 Std. Deviation 180,25809 172,2403
a,b
39 Normal Parameters
39
41
41
KGD_hari_k e_9 KGD_hari_ke_1
KGD_normal KGD_hari_ke_1 KGD_hari_ke_3 N
KGD_hari_k e_5 KGD_ha ri_ke_7
One-Sample Kolmogorov-Smirnov Test
Kolmogorov-Smirnov Z ,494 1,899 1,577 Asymp. Sig. (2-tailed) ,968 ,001 ,014
Most Extreme Differences Absolute ,076 ,293 ,243 Positive ,061 ,172 ,159 Negative -,076 -,293 -,243
Mean 98,3810 374,8333 347,5238 Std. Deviation 11,63668 211,13606 198,96859
a,b
42 Normal Parameters
42
42
Analisis data dengan menggunakan Anova satu arah
ANOVA Sum of Squares df Mean Square F Sig.
KGD_normal Between Groups 1367,905 6 227,984 1,907 ,107 Within Groups 4184,000 35 119,543 Total 5551,905
41 KGD_hari_ke_1 Between Groups 1765346,667 6 294224,444 165,111 ,000 Within Groups 62369,167 35 1781,976 Total 1827715,833
41 KGD_hari_ke_3 Between Groups 1538411,476 6 256401,913 105,930 ,000 Within Groups 84717,000 35 2420,486 Total 1623128,476
41 KGD_hari_ke_5 Between Groups 1241475,822 6 206912,637 120,787 ,000 Within Groups 58243,300 34 1713,038 Total 1299719,122
40 KGD_hari_ke_7 Between Groups 1122279,420 6 187046,570 98,767 ,000 Within Groups 64389,800 34 1893,818 Total 1186669,220
40 KGD_hari_ke_9 Between Groups 784049,808 6 130674,968 82,717 ,000 Within Groups 50553,167 32 1579,786 Total 834602,974
38 KGD_hari_ke_11 Between Groups 499256,077 6 83209,346 66,353 ,000 Within Groups 40129,000 32 1254,031 Total 539385,077
38 Uji lanjutan rerata dengan menggunakan uji Duncan
KGD_normal a
Duncan Subset for alpha = 0.2
Perlakuan N
1
2
3 Preventif, EEDSM 100 6 89,6667 mg/kg BB EEDSM 200 mg/kg BB 6 94,1667 94,1667 metformin 10 mg/kg BB
6 97,8333 97,8333 Kontrol negatif, CMC 0,5% 6 98,3333 98,3333 EEDSM 50 mg/kg BB 6 98,8333 98,8333 EEDSM 100 mg/kg BB 6 100,0000 base line
6 109,8333 Sig. ,204 ,418 1,000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6,000.
KGD_hari_ke_1 a
Duncan Subset for alpha = 0.2
Perlakuan N
1
2
3
4
5 Preventif, EEDSM 100 6 ,0000 mg/kg BB base line 6 110,8333 EEDSM 100 mg/kg BB 6 470,6667 EEDSM 200 mg/kg BB 6 482,8333 482,8333 Kontrol negatif, CMC 0,5% 6 513,3333 513,3333 metformin 10 mg/kg BB
6 518,5000
EEDSM 50 mg/kg BB
6 527,6667 Sig. 1,000 1,000 ,621 ,219 ,585 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6,000.
KGD_hari_ke_3 a
Duncan Subset for alpha = 0.2
Perlakuan N
1
2
3
4
5
6 Preventif, EEDSM 100 mg/kg 6 ,0000 BB base line
6 113,1667 EEDSM 200 mg/kg BB 6 394,5000 metformin 10 mg/kg BB
6 439,8333 EEDSM 100 mg/kg BB 6 453,5000 453,5000 EEDSM 50 mg/kg BB
6 483,5000
Kontrol negatif, CMC 0,5%
6 548,1667
Sig. 1,000 1,000 1,000 ,633 ,298 1,000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6,000.
KGD_hari_ke_5 a,b
Duncan Subset for alpha = 0.2
Perlakuan N
1
2
3
4
5
6 Preventif, EEDSM 100 mg/kg 6 ,0000 BB base line 6 110,8333 metformin 10 mg/kg BB 6 341,1667
EEDSM 200 mg/kg BB 6 347,8333 EEDSM 100 mg/kg BB 6 406,0000 EEDSM 50 mg/kg BB
1
5 552,2000
6 263,3333 EEDSM 200 mg/kg BB 6 288,0000 EEDSM 100 mg/kg BB 6 341,5000 EEDSM 50 mg/kg BB 6 399,8333 Kontrol negatif, CMC 0,5%
6 Preventif, EEDSM 100 mg/kg BB 6 ,0000 base line 6 110,3333 metformin 10 mg/kg BB
5
4
3
2
Perlakuan N Subset for alpha = 0.2
6 438,0000
a,b
Duncan
KGD_hari_ke_7
b. The group sizes are unequal. The harmonic mean of the group sizes is used. Type I error levels are not guaranteed.
a. Uses Harmonic Mean Sample Size = 5,833.
Sig. 1,000 1,000 ,785 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed.
5 537,8000
Kontrol negatif, CMC 0,5%
Sig. 1,000 1,000 ,340 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed.
KGD_hari_ke_5 a,b
Duncan Subset for alpha = 0.2
Perlakuan N
1
2
3
4
5
6 Preventif, EEDSM 100 mg/kg 6 ,0000 BB base line
6 110,8333 metformin 10 mg/kg BB 6 341,1667
EEDSM 200 mg/kg BB 6 347,8333 EEDSM 100 mg/kg BB 6 406,0000 EEDSM 50 mg/kg BB
6 438,0000
Kontrol negatif, CMC 0,5%
5 537,8000
Sig. 1,000 1,000 ,785 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,833.
b. The group sizes are unequal. The harmonic mean of the group sizes is used. Type I error levels are not guaranteed.
KGD_hari_ke_9 a,b
Duncan Subset for alpha = 0.2
Perlakuan N
1
2
3
4
5
6 Preventif, EEDSM 100 mg/kg 6 ,000 BB base line
6 113,500 metformin 10 mg/kg BB 6 219,000
EEDSM 200 mg/kg BB 6 227,000 EEDSM 100 mg/kg BB 6 282,500
EEDSM 50 mg/kg BB 6 357,500 Kontrol negatif, CMC 0,5%
3 530,667
Sig. 1,000 1,000 ,746 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,250.
b. The group sizes are unequal. The harmonic mean of the group sizes is used. Type I error levels are not guaranteed.
KGD_hari_ke_11 a,b
Duncan Subset for alpha = 0.2
Perlakuan N
1
2
3
4
5
6 base line 6 109,3333
EEDSM 200 mg/kg BB 6 145,6667 metformin 10 mg/kg BB 6 171,8333 171,8333
Preventif, EEDSM 100 mg/kg 6 183,3333 183,3333 BB EEDSM 100 mg/kg BB 6 210,5000 EEDSM 50 mg/kg BB
6 320,0000
Kontrol negatif, CMC 0,5%
3 541,6667
Sig. 1,000 ,240 ,602 ,223 1,000 1,000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,250.
b. The group sizes are unequal. The harmonic mean of the group sizes is used. Type I error levels are not guaranteed.
Lampiran 12. Analisis data statistik berat badan mencit diabetes yang diinduksi aloksan
Analisis normalitas data dengan metode uji Kolmogorov-Smirnov
42
41
Kolmogorov-Smirnov Z 2,260 2,105 ,566 Asymp. Sig. (2-tailed) ,000 ,000 ,906
Most Extreme Differences Absolute ,353 ,337 ,091 Positive ,202 ,168 ,086 Negative -,353 -,337 -,091
Mean 27,1659 26,9769 32,4821 Std. Deviation 11,68042 12,06597 4,13011
a,b
39 Normal Parameters
39
BB_hari_ke_7 BB_hari_ke_9 BB_hari_ke_11 N
42
BB_awal BB_hari_ke_1 BB_hari_ke_3 BB_hari_ke_5 N
Most Extreme Differences Absolute ,121 ,296 ,343 ,381 Positive ,121 ,189 ,205 ,233 Negative -,069 -,296 -,343 -,381
Mean 33,3571 27,0786 26,4214 26,7171 Std. Deviation 3,86259 11,58958 11,13961 11,36439
a,b
41 Normal Parameters
42
Kolmogorov-Smirnov Z ,784 1,922 2,223 2,441 Asymp. Sig. (2-tailed) ,570 ,001 ,000 ,000 Analisis data dengan menggunakan Anova satu arah ANOVA Sum of Squares df Mean Square F Sig.
BB_awal Between Groups 390,853 6 65,142 10,324 ,000 Within Groups 220,850 35 6,310 Total 611,703
41 BB_hari_ke_1 Between Groups 5294,399 6 882,400 145,233 ,000 Within Groups 212,652 35 6,076 Total 5507,051
41 BB_hari_ke_3 Between Groups 4915,566 6 819,261 166,550 ,000 Within Groups 172,165 35 4,919 Total 5087,731
41 BB_hari_ke_5 Between Groups 5075,859 6 845,977 319,170 ,000 Within Groups 90,119 34 2,651 Total 5165,978
40 BB_hari_ke_7 Between Groups 5342,734 6 890,456 264,281 ,000 Within Groups 114,558 34 3,369 Total 5457,292
40 BB_hari_ke_9 Between Groups 5290,553 6 881,759 116,704 ,000 Within Groups 241,777 32 7,556 Total 5532,329
38 BB_hari_ke_11 Between Groups 236,684 6 39,447 3,067 ,017 Within Groups 411,513 32 12,860 Total 648,197
38 Uji lanjutan rerata dengan menggunakan uji Duncan
BB_awal
a. Uses Harmonic Mean Sample Size = 6,000.
3 Preventif, EEDSM 100 mg/kg BB 6 ,0000 base line 6 29,5000 EEDSM 100 mg/kg BB 6 30,2167 metformin 10 mg/kg BB
2
1
Perlakuan N Subset for alpha = 0.2
a
Duncan
BB_hari_ke_1
6 34,7500 34,7500 EEDSM 50 mg/kg BB 6 35,8667 35,8667 Kontrol negatif, CMC 0,5% 6 37,4833 Sig. 1,000 ,455 ,229 ,273 Means for groups in homogeneous subsets are displayed.
Duncan
Preventif, EEDSM 100 mg/kg BB 6 28,9167 EEDSM 100 mg/kg BB 6 33,5833 EEDSM 200 mg/kg BB 6 33,9833 33,9833 metformin 10 mg/kg BB
4 base line 6 28,9167
3
2
1
Perlakuan N Subset for alpha = 0.2
a
6 30,3333 EEDSM 200 mg/kg BB 6 30,3833 EEDSM 50 mg/kg BB 6 34,4333 Kontrol negatif, CMC 0,5% 6 34,6833 Sig. 1,000 ,578 ,862 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6,000.
Lampiran 13. Analisis data statistik perubahan berat badan mencit diabetes yang diinduksi aloksan Analisis normalitas data dengan metode uji Kolmogorov-Smirnov One-Sample Kolmogorov-Smirnov Test
Perubahan_BB N
42 Normal Parameters
a,b
Mean -,2690 Std. Deviation 15,03043
Most Extreme Differences Absolute ,101 Positive ,087 Negative -,101
Kolmogorov-Smirnov Z ,652 Asymp. Sig. (2-tailed) ,789 a. Test distribution is Normal.
b. Calculated from data.
Analisis data dengan menggunakan Anova satu arah ANOVA
Perubahan_BB Sum of Squares df Mean Square F Sig.
Between Groups 5160,508 6 860,085 7,339 ,000 Within Groups 4101,962 35 117,199 Total 9262,470
Perubahan_BB a
Duncan Subset for alpha = 0.2
Perlakuan N
1
2
3
4 Kontrol negatif, CMC 0,5% 6 -12,4167 EEDSM 50 mg/kg BB 6 -12,0167 metformin 10 mg/kg BB
6 -10,2500 EEDSM 100 mg/kg BB 6 -1,3000 EEDSM 200 mg/kg BB 6 7,4500 Preventif, EEDSM 100 mg/kg 6 8,6000 BB base line
6 18,0500 Sig. ,747 1,000 ,855 1,000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6,000.
Lampiran 14. Analisis data statistik berat pankreas mencit diabetes yang diinduksi aloksan Analisis normalitas data dengan metode uji Kolmogorov-Smirnov One-Sample Kolmogorov-Smirnov Test
Berat_Pankreas N
39 Normal Parameters
a,b
Mean 164,72 Std. Deviation 30,527
Most Extreme Differences Absolute ,124 Positive ,124 Negative -,091
Kolmogorov-Smirnov Z ,776 Asymp. Sig. (2-tailed) ,583 a. Test distribution is Normal.
b. Calculated from data.
Analisis data dengan menggunakan Anova satu arah ANOVA
Berat_Pankreas Sum of Squares df Mean Square F Sig.
Between Groups 21918,731 6 3653,122 8,664 ,000 Within Groups 13493,167 32 421,661 Total 35411,897
Berat_Pankreas
Duncan
a,b
Subset for alpha = 0.2 Perlakuan N
1
2
3
4 Kontrol negatif, CMC 0,5% 3 129,00 metformin 10 mg/kg BB 6 140,33 base line 6 146,00
Preventif, EEDSM 100 mg/kg 6 164,50 BB EEDSM 50 mg/kg BB 6 165,50 EEDSM 100 mg/kg BB 6 183,83 EEDSM 200 mg/kg BB 6 206,00 Sig. ,215 ,938 1,000 1,000 Means for groups in homogeneous subsets are displayed. Lampiran 15. Perhitungan luas pulau langerhans pankreas Pada perhitungan luas pulau langerhans di ambil contoh pada preparat histologi pankreas kelompok normal Perhitungan luas pulau langerhans pankreas mencit menggunakan persamaan matematika : ½ π x jari-jari minor x jari-jari mayor
40 µm 131 µm
Jari-jari mayor = ½ x diameter mayor = ½ x 131 µm = 65,5 µm Jari-jari minor = ½ x diameter minor = ½ x 40 µm = 20 µm Luas pulau langerhans adalah = ½ π jari-jari minor x jari-jari mayor
2 ½ π x 20 x65,5 = 2058,5 µm
Lampiran 16. Luas pulau langerhans pankreas mencit yang diberi ekstrak daun sirih merah
EEDSM 50 mg/kg BB
56 36 28,2 17,8 789,2
6
39 25 19,6 12,4 381,9
5
42 26 20,8 13,2 431,2
4
59 37 29,4 18,6 859,3
3
64 40 31,9 20,2 1008,5
2
49 31 24,5 15,5 596,7
1
Perlakuan n diameter (µm) jari-jari (µm) luas mayor minor mayor minor base line 1 131
40 65,5 20,0 2058,6 2 170 52 85,2 26,0 3479,0 3 157 48 78,6 24,0 2964,3 4 111 34 55,7 17,0 1487,3 5 105 32 52,4 16,0 1317,5 6 151 46 75,3 23,0 2722,5 purata
6
30 11 14,8 5,6 130,2
5
31 12 15,7 6,0 147,0
4
44 17 22,2 8,4 293,0
3
48 18 24,1 9,1 343,9
2
37 14 18,5 7,0 203,5
1
Kontrol negatif/CMC 0,5%
2338,2 sd 858,5
43 16 21,3 8,1 269,1 purata 231,1 sd 84,9 Perlakuan n diameter (µm) jari-jari (µm) luas mayor minor mayor minor purata
677,8 sd 248,9
2
61 59 30,6 29,3 1410,1
5
58 55 28,8 27,6 1249,1
6
83 79 41,4 39,7 2581,1 purata 2216,8 sd 813,9 metformin 10 mg/kg BB
1
42 37 21,0 18,5 610,5
55 48 27,3 24,1 1031,7
86 83 43,2 41,4 2810,5
3
50 44 25,2 22,2 879,1
4
36 31 17,9 15,7 441,1
5
34 30 16,8 14,8 390,7
6
4
3
EEDSM 100 mg/kg BB
47 33 23,4 16,6 608,8
1
55 39 27,5 19,5 842,7
2
72 51 35,8 25,4 1424,1
3
66 47 33,0 23,4 1213,5
4
5
94 90 46,8 44,9 3298,4
44 31 22,0 15,6 539,3
6
63 45 31,6 22,4 1114,4 purata 957,1 sd 351,4
EEDSM 200 mg/kg BB
1
72 69 36,0 34,5 1951,7
2
48 43 24,2 21,3 807,4 Perlakuan n diameter (µm) jari-jari (µm) luas mayor minor mayor minor purata
693,4 sd 254,6
EEDSM 100 mg/kg BB, preventif 1 116 51 58,0 25,5 2324,1 2 151
66 75,4 33,2 3927,8 3 139 61 69,6 30,6 3346,8
4
99 43 49,3 21,7 1679,2
5
93 41 46,4 20,4 1487,5 6 133 59 66,7 29,3 3073,7 purata
2639,8 sd 969,3 Lampiran 17. Analisis statistik luas pulau langerhans pankreas yang diberi ekstrak daun sirih merah
Analisis normalitas dengan metode Kolmogorov-Smirnov
One-Sample Kolmogorov-Smirnov Test
luas_pulau_langerh ans N
42
a,b
Normal Parameters Mean 1393,4762 Std. Deviation 1070,53435
Most Extreme Differences Absolute ,155 Positive ,155 Negative -,119
Kolmogorov-Smirnov Z 1,008 Asymp. Sig. (2-tailed) ,262 a. Test distribution is Normal.
b. Calculated from data.
Analisis Anova dan uji beda rerata Duncan
ANOVA
luas_pulau_langerhans Sum of Squares df Mean Square F Sig.
Between Groups 34005544,723 6 5667590,787 15,280 ,000 Within Groups 12982250,873 35 370921,454 Total 46987795,596
41 a
Duncan Subset for alpha = 0.2
Perlakuan N
1
2
3 Kontrol negatif, CMC 0,5% 6 231,1167 EESM 50 mg/kg BB 6 677,8000 677,8000 metformin 10 mg/kg BB
6 693,4167 693,4167 EESM 100 mg/kg BB 6 957,1333 EESM 200 mg/kg BB 6 2216,8167 base line
6 2338,2000 Preventif, EESM 100 mg/kg BB 6 2639,8500 Sig. ,223 ,460 ,265 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6,000.