Embriogenesis Somatik Dari Bunga Betina Kelapa Sawit (Elaeis guineensis Jacq.)

44

Lampiran 1. Tabel Komposisi Medium Y3 Yang Digunakan

Makro Elemen
NH4 CL
KNO3
Mg SO4. 7 H2O
CaCl 2.2 H2 O
KCl
NaH2PO4

Gram/L
535
2020
247
294
1492
312

Mikro Elemen


Gram/L

KI

8,3

H3BO3

3,1

Mn SO4. 4 H2O

11,2

Zn SO4. 7 H2O

7,2

CuSO4.5H2O


0,25

CoCl2. 6H2O

0,24

NaMoO4. H2O

0,24

NiCl2.6H2O

0,024

Fe2So4. 7H2O

41,7

Na2EDTA


55,8

Piridoxin Hcl

0,05

Tiamine Hcl

0,05

Gula

60

44

45

Lampiran 2. Data Pengamatan Induksi Kalus Primer

Ulangan
Perlakuan
K1P1
K2P1
K3P1
K4P1
K1P2
K2P2
K3P2
K4P2
K1P3
K2P3
K3P3
K4P3

I

II

III


IV

V

0
1
1
1
0
0
0
1
0
1
0
0
Jumlah

0

1
1
1
0
1
0
0
0
1
0
0

1
0
1
1
0
0
0
1

0
1
0
0

0
1
1
1
0
1
0
0
0
1
0
0

0
0

1
1
0
0
0
0
0
0
0
0

45

Total
1
3
5
5
0
2

0
2
0
4
0
0
22

Persentase
Perlakuan Pada
Posisi Eksplan
Yang Sama
7.14%
21.43%
35.71%
35.71%
0.00%
14.29%
0.00%
14.29%

0.00%
28.57%
0.00%
0.00%

Persentase
Total Antar
Posisi
Eksplan
64%

18%

18%

46

Lampiran 3. Data Pengamatan Induksi Kalus Embriogenik
Perlakuan
K1P1

K2P1
K3P1
K4P1
K1P2
K2P2
K3P2
K4P2
K1P3
K2P3
K3P3
K4P3

I II
0 0
1 1
1 1
1 1
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
Jumlah

Ulangan
III IV
1
0
0
1
1
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0

V
0
0
1
1
0
0
0
0
0
0
0
0

46

Total
1
3
5
5
0
0
0
0
0
0
0
0
14

Persentase
Posisi

100%

0%

0%

47

Lampiran 4. Data Berat Basah Kultur

Perlakuan
K1P1
K2P1
K3P1
K4P1

I
0.032
0.032
0.12
0.183

II
0.067
0.045
0.133
0.148

Ulangan
III
IV
0.042
0.07
0.07
0.09
0.181 0.181
0.206 0.136

47

V
0.037
0.045
0.107
0.18

Rata Rata
0.050
0.056
0.144
0.171

Total
0.248
0.282
0.722
0.853

48

Lampiran 5. Data Berat Kering Kultur

Perlakuan
K1P1
K2P1
K3P1
K4P1

I
0.008
0.011
0.018
0.016

II
0.005
0.007
0.018
0.011

Ulangan
III
IV
0.002 0.006
0.008 0.008
0.015
0.01
0.018 0.016

48

V
0.004
0.002
0.011
0.01

Rata Rata
0.005
0.007
0.014
0.014

Total
0.025
0.036
0.072
0.071

49

Lampiran 6. Master Tabel SPSS Saat Terbentuknya Kalus Primer
Ulangan Terbentuknya
Kalus Primer

No

Faktor K

Faktor P

1

K1

P1

1

2

K1

P1

3

K1

4

Faktor P Ulangan Terbentuknya
Kalus Primer

No

Faktor K

0

36

K3

P2

1

0

2

0

37

K3

P2

2

0

P1

3

1

38

K3

P2

3

0

K1

P1

4

0

39

K3

P2

4

0

5

K1

P1

5

0

40

K3

P2

5

0

6

K1

P2

1

0

41

K3

P3

1

0

7

K1

P2

2

0

42

K3

P3

2

0

8

K1

P2

3

0

43

K3

P3

3

0

9

K1

P2

4

0

44

K3

P3

4

0

10

K1

P2

5

0

45

K3

P3

5

0

11

K1

P3

1

0

46

K4

P1

1

1

12

K1

P3

2

0

47

K4

P1

2

1

13

K1

P3

3

0

48

K4

P1

3

1

14

K1

P3

4

0

49

K4

P1

4

1

15

K1

P3

5

0

50

K4

P1

5

1

16

K2

P1

1

1

51

K4

P2

1

1

17

K2

P1

2

1

52

K4

P2

2

0

18

K2

P1

3

0

53

K4

P2

3

1

19

K2

P1

4

1

54

K4

P2

4

0

20

K2

P1

5

0

55

K4

P2

5

0

21

K2

P2

1

0

56

K4

P3

1

0

22

K2

P2

2

1

57

K4

P3

2

0

23

K2

P2

3

0

58

K4

P3

3

0

24

K2

P2

4

1

59

K4

P3

4

0

25

K2

P2

5

0

60

K4

P3

5

0

26

K2

P3

1

1

27

K2

P3

2

1

28

K2

P3

3

1

29

K2

P3

4

1

30

K2

P3

5

0

31

K3

P1

1

1

32

K3

P1

2

1

33

K3

P1

3

1

34

K3

P1

4

1

35

K3

P1

5

1

49

50

Lampiran 7. Master Tabel SPSS Saat Terbentuknya Kalus Embriogenik

No Faktor A Faktor B Ulangan

Terbentuknya
Kalus
Embriogenik

No Faktor A Faktor B Ulangan

Terbentuknya
Kalus
Embriogenik

1

K1

P1

1

0

36

K3

P2

1

0

2

K1

P1

2

0

37

K3

P2

2

0

3

K1

P1

3

1

38

K3

P2

3

0

4

K1

P1

4

0

39

K3

P2

4

0

5

K1

P1

5

0

40

K3

P2

5

0

6

K1

P2

1

0

41

K3

P3

1

0

7

K1

P2

2

0

42

K3

P3

2

0

8

K1

P2

3

0

43

K3

P3

3

0

9

K1

P2

4

0

44

K3

P3

4

0

10

K1

P2

5

0

45

K3

P3

5

0

11

K1

P3

1

0

46

K4

P1

1

1

12

K1

P3

2

0

47

K4

P1

2

1

13

K1

P3

3

0

48

K4

P1

3

1

14

K1

P3

4

0

49

K4

P1

4

1

15

K1

P3

5

0

50

K4

P1

5

1

16

K2

P1

1

1

51

K4

P2

1

0

17

K2

P1

2

1

52

K4

P2

2

0

18

K2

P1

3

0

53

K4

P2

3

0

19

K2

P1

4

1

54

K4

P2

4

0

20

K2

P1

5

0

55

K4

P2

5

0

21

K2

P2

1

0

56

K4

P3

1

0

22

K2

P2

2

0

57

K4

P3

2

0

23

K2

P2

3

0

58

K4

P3

3

0

24

K2

P2

4

0

59

K4

P3

4

0

25

K2

P2

5

0

60

K4

P3

5

0

26

K2

P3

1

0

27

K2

P3

2

0

28

K2

P3

3

0

29

K2

P3

4

0

30

K2

P3

5

0

31

K3

P1

1

1

32

K3

P1

2

1

33

K3

P1

3

1

34
35

K3
K3

P1
P1

4
5

1
1

50

51

Lampiran 8. Master Tabel SPSS Untuk Berat Basah Kalus
No

Faktor K

Faktor P

Ulangan

Berat Basah

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20

K1
K1
K1
K1
K1
K2
K2
K2
K2
K2
K3
K3
K3
K3
K3
K4
K4
K4
K4
K4

P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1

1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5

0.032
0.067
0.042
0.07
0.037
0.032
0.045
0.07
0.09
0.045
0.12
0.133
0.181
0.181
0.107
0.183
0.148
0.206
0.136
0.18

51

52

Lampiran 9. Master Tabel SPSS Untuk Berat Kering Kalus
No

Faktor A

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20

K1
K1
K1
K1
K1
K2
K2
K2
K2
K2
K3
K3
K3
K3
K3
K4
K4
K4
K4
K4

Faktor B
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1
P1

Ulangan

Berat Basah

1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5

0.008
0.005
0.002
0.006
0.004
0.011
0.007
0.008
0.008
0.002
0.018
0.018
0.015
0.01
0.011
0.016
0.011
0.018
0.016
0.01

52

53

Lampiran 10. Data Output SPSS Induksi Kalus Primer
Analisis Sidik Ragam Saat Terbentuknya Kalus Primer
Variabel Bebas : Kalus Primer
Type III Sum
Source
df
Mean Square
of Squares
Corrected Model
8.733a
11
.794
Intercept
8.067
1
8.067
Faktor_A
2.333
3
.778
Faktor_B
3.333
2
1.667
Faktor_A *
3.067
6
.511
Faktor_B
Error
5.200
48
.108
Total
22.000
60
Corrected Total
13.933
59
a. R Squared = .627 (Adjusted R Squared = .541)

F

Sig.

7.329
74.462
7.179
15.385

.000
.000
.000
.000

4.718

.001

Uji DMRT (Duncan Multiple Range Test)
Kalus Primer
Konsentrasi 2.4-D
Duncan

N

1
.0667

a,,b

Subset
2

33mg/l
15
99mg/l
15
.3333
132mg/l
15
.4667
66mg/l
15
Sig.
1.000
.273
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = .108.
a. Uses Harmonic Mean Sample Size = 15.000.
b. Alpha = .05.

53

3

.4667
.6000
.273

54

Kalus Primer
Subset
Posisi
N
Eksplan
1
2
a,,b
Duncan
Apikal
20
.2000
Median
20
.2000
Basal
20
.7000
Sig.
1.000
1.000
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = .108.
a. Uses Harmonic Mean Sample Size = 20.000.
b. Alpha = .05.

54

55

Lampiran 11. Data Output SPSS Kalus Embriogenik
Analisis Saat Terbentuknya Kalus Embriogenik

ANOVA
Kalus Embriogenik

Between Groups
Within Groups
Total

Sum of
Squares
2.200
2.000
4.200

Df

Mean Square

F

Sig.

3
16
19

.733
.125

5.867

.007

Uji DMRT (Duncan Multiple Range Test)
Kalus Embriogenik
Duncan

a

Subset for alpha = 0.05
1
2
33mg/l
5
.2000
66mg/l
5
.6000
.6000
99mg/l
5
1.0000
132mg/l
5
1.0000
Sig.
.093
.108
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Konsentrasi 2.4-D

N

55

56

Lampiran 12. Data Output SPSS Pada Berat Basah Kultur

Analisis Sidik Ragam Berat Basah Kultur

ANOVA
Kalus Primer

Between Groups
Within Groups
Total

Sum of
Squares
.056
.011
.068

Df

Mean Square

F

Sig.

3
16
19

.019
.001

26.353

.000

Uji DMRT (Duncan Multiple Range Test)

Berat Basah Kultur
Duncan

a

Subset for alpha = 0.05
1
2
33mg/l
5
.04960
66mg/l
5
.05640
99mg/l
5
.14440
132mg/l
5
.17060
Sig.
.693
.141
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Konsentrasi 2,4-D

N

56

57

Lampiran 13. Data Output SPSS Pada Berat Kering Kultur

Analisis Sidik Ragam Berat Kering Kultur

ANOVA
Kalus Primer
Sum of
Squares
.000
.000
.001

Between Groups
Within Groups
Total

Df

Mean Square

F

Sig.

3
16
19

.000
.000

11.008

.000

Uji DMRT (Duncan Multiple Range Test)

Berat Kering Kultur
Duncan

a

Subset for alpha = 0.05
1
2
33mg/l
5
.00500
66mg/l
5
.00720
132mg/l
5
.01420
99mg/l
5
.01440
Sig.
.300
.924
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Konsentrasi 2,4-D

N

57