THE EFFECT OF PHERETIMA ASPERGILLUM EXTRACT ON THE INCREASE OF HAIR FOLLICLES NUMBER IN DIABETIC ULCER GRADE II IN WISTAR RAT | Kristianto | Jurnal Luka Indonesia 1 SM
Jurnal Luka Indonesia Vol.2(2): Juni-September 2016
ISSN: 2442-2665
THE EFFECT OF PHERETIMA
ASPERGILLUM EXTRACT ON THE
INCREASE OF HAIR FOLLICLES NUMBER
IN DIABETIC ULCER GRADE II IN WISTAR
ORAL PRESENTATION
How to cite:
Kristianto Heri ,
Sari Astika, The Effect Of
Pheretima Aspergillum Extract
On The Increase Of Hair
Follicles Number In Diabetic
Ulcer Grade Ii In Wistar Rat
Jurnal luka Indonesia. 2016,
2(2):102
.
Heri Kristianto1,Dwi Astika Sari2
1
Conflict of interest:
Nothing
Funding resources:
Nothing
Corresponding authors:
[email protected]
Note:
Spesialis Keperawatan Medikal Bedah, Jurusan Keperawatan, Fakultas
Kedokteran, Universitas Brawijaya, Praktisi luka, kaki dan educator diabetes
(Diafoss Care Center)
2
Mahasiswa Program Studi Ilmu Keperawatan, Fakultas Kedokteran,
Universitas Brawijaya
ABSTRACT
Background: Diabetic ulcer is the result of serious microvascular
complication of diabetes mellitus that is caused by vascular insufficiency,
resulting in dry skin, loss of taste, and a decrease in the number of hair
follicles. Earthworm (Pheretima aspergillum) has a bioactive component of
IGF-1 (insulin like growth factor 1), which can stimulate cell growth and
survival. IGF-1 is involved in hair follicle growth by regulating cell
proliferation and migration through the cellular mechanism of its receptor
(IGF - 1R). This study aimed to determine the effect of earthworm extract on
the increase of hair follicles number in rats induced diabetic ulcer stage II.
Methods: This study used a true experimental posttest only controlled
group design. Samples were randomly selected, divided into five groups:
Normal Saline as a negative control group, Hydrogel as a positive control
group, and the three treatment groups received earthworm extract via
topical, oral, and topical-oral. Ulcer treatment is done for 21 days, on day 22,
rats were euthanized. The numbers of hair follicles in skin tissue connecting
to nervous fibers were identified using silver impregnation stain.
Results: The results showed that the treatment groups that received
earthworm extract had significantly higher number of hair follicles as
compared to the control groups (p=0.000). The administration of earthworm
extract via topical has an optimal effect on the increase of hair follicles
numbers.
Conclusion: It can be concluded that the extract of earthworm (Pheretima
aspergillum) can increase the growth of hair follicles in rats diabetic models.
Keywords: diabetic ulcer, earthworm (Pheretima aspergillum), hair
follicles.
102
ISSN: 2442-2665
THE EFFECT OF PHERETIMA
ASPERGILLUM EXTRACT ON THE
INCREASE OF HAIR FOLLICLES NUMBER
IN DIABETIC ULCER GRADE II IN WISTAR
ORAL PRESENTATION
How to cite:
Kristianto Heri ,
Sari Astika, The Effect Of
Pheretima Aspergillum Extract
On The Increase Of Hair
Follicles Number In Diabetic
Ulcer Grade Ii In Wistar Rat
Jurnal luka Indonesia. 2016,
2(2):102
.
Heri Kristianto1,Dwi Astika Sari2
1
Conflict of interest:
Nothing
Funding resources:
Nothing
Corresponding authors:
[email protected]
Note:
Spesialis Keperawatan Medikal Bedah, Jurusan Keperawatan, Fakultas
Kedokteran, Universitas Brawijaya, Praktisi luka, kaki dan educator diabetes
(Diafoss Care Center)
2
Mahasiswa Program Studi Ilmu Keperawatan, Fakultas Kedokteran,
Universitas Brawijaya
ABSTRACT
Background: Diabetic ulcer is the result of serious microvascular
complication of diabetes mellitus that is caused by vascular insufficiency,
resulting in dry skin, loss of taste, and a decrease in the number of hair
follicles. Earthworm (Pheretima aspergillum) has a bioactive component of
IGF-1 (insulin like growth factor 1), which can stimulate cell growth and
survival. IGF-1 is involved in hair follicle growth by regulating cell
proliferation and migration through the cellular mechanism of its receptor
(IGF - 1R). This study aimed to determine the effect of earthworm extract on
the increase of hair follicles number in rats induced diabetic ulcer stage II.
Methods: This study used a true experimental posttest only controlled
group design. Samples were randomly selected, divided into five groups:
Normal Saline as a negative control group, Hydrogel as a positive control
group, and the three treatment groups received earthworm extract via
topical, oral, and topical-oral. Ulcer treatment is done for 21 days, on day 22,
rats were euthanized. The numbers of hair follicles in skin tissue connecting
to nervous fibers were identified using silver impregnation stain.
Results: The results showed that the treatment groups that received
earthworm extract had significantly higher number of hair follicles as
compared to the control groups (p=0.000). The administration of earthworm
extract via topical has an optimal effect on the increase of hair follicles
numbers.
Conclusion: It can be concluded that the extract of earthworm (Pheretima
aspergillum) can increase the growth of hair follicles in rats diabetic models.
Keywords: diabetic ulcer, earthworm (Pheretima aspergillum), hair
follicles.
102