Uji Antihiperkolesterolemia Ekstrak Etil Asetat Daun Binahong (Anredera cordifolia (Ten.) Steenis) pada Serum Darah Marmot
Uji Antihiperkolesterolemia Ekstrak Etil Asetat Daun Binahong ( Anredera
Cordifolia (Ten.) Steenis) pada Serum Darah Marmot
ABSTRAK
Binahong (Anredera cordifolia (Ten.) Steenis) merupakan tumbuhan
menjalar yang memiliki banyak manfaat untuk mengobati berbagai penyakit,
seperti menurunkan kolesterol. Kolesterol merupakan faktor pembentukan
aterosklerosis arteri-arteri vital, yang menimbulkan penyakit pembuluh darah
perifer, koroner, dan serebrovaskular. Tujuan penelitian ini adalah mengetahui
pengaruh pemberian ekstrak etil asetat daun binahong terhadap kadar kolesterol
darah marmot yang dibuat hiperkolesterolemia, dan membandingkan efek
penurunan kolesterol dari ekstrak etil asetat daun binahong dengan obat
simvastatin.
Sampel daun binahong dikarakterisasi dan dilakukan skrining fitokimia,
sebelum diuji sebagai penurunan kolesterol marmot. Marmot hiperkolesterolemia
dibuat dengan memberikan makanan induksi kuning telur (dosis 1% bb) dicampur
lemak kambing 15 g selama 14 hari. Suspensi ekstrak etil asetat daun binahong
diberikan per oral dengan dosis 100 mg/kgbb, 200 mg/kgbb, dan 400 mg/kgbb,
sebagai kontrol positif adalah simvastatin dan CMC Na sebagai kontrol pelarut
selama 7 hari pada marmot yang mengalami hiperkolesterolemia.
Hasil pemeriksaan karakterisasi simplisia daun binahong yaitu kadar air
7,32%, kadar sari larut air 26,16%, kadar sari larut etanol 25,86%, kadar abu total
6,35%, dan kadar abu tidak larut asam 0,58%. Simplisia daun binahong
mengandung senyawa metabolit sekunder alkaloid, glikosida, flavonoid, tannin,
saponin dan steroid. Hasil penelitian menunjukkan ekstrak etil asetat daun
binahong 400 mg/kgbb memberikan efek penurunan kadar kolesterol yang tidak
berbeda nyata (p ≤ 0,05) dengan pemberian simvastatin, dibandingkan dosis 100
mg/kgbb dan 200 mg/kgbb menurunkan kolesterol tidak lebih baik dari
simvastatin. Disimpulkan bahwa pemberian ekstrak etil asetat daun binahong
dosis 400 mg/kgbb merupakan dosis efektif untuk penurunan kadar kolesterol
serum pada marmot hiperkolesterolemia.
Kata
Kunci:
(Anredera cordifolia (Ten.) Steenis),
Karakterisisasi simplisia, Skrining fitokimia,
Hiperkolesterolemia
vii
Universitas Sumatera Utara
Anti-Hypercholesterolemia of Ethyl Acetate Extract of the Binahong Leaves
(Anredera cordifolia (Ten.) Steenis) in Blood Serum Guinea Pig
ABSTRACT
Binahong (Anredera cordifolia (Ten.) Steenis) are vines that have many
benefits for treating various diseases, such as to lower cholesterol. Cholesterol is a
factor of the formation of atherosclerosis vital arteries, causing peripheral vascular
disease, coronary and cerebrovascular. The purpose of this research was to
determine the effect of ethyl acetate extract of the binahong leaves on blood
cholesterol levels guinea pig made hypercholesterolemia, and comparing the
cholesterol lowering effect of ethyl acetate extract of the binahong leaves with
simvastatin drug.
Sample of binahong leaf were characterized and phytochemical screening
done before in trials as a cholesterol-lowering effect of a guinea pig. Guinea pig
hypercholesterolemia made with food induced yolk (dose 1% body weight) mixed
with goat fat 15 g for 14 days. Suspension of ethyl acetate extract of the leaves
binahong given orally at a dose of 100 mg/kg body weight, 200 mg/kg body
weight, 400 mg/kg body weight, a positive control is simvastatin and CMC Na as
a solvent control For 7 days in guinea pig who have hypercholesterolemia.
The result of simplex characterization of binahong leaves is the water
content value 7.32%, the water soluble extract value 26.16%, the ethanol soluble
extract value 25.86%, the total ash 6.35%, and the ash is not soluble in acid
0.58%. Simplex of binahong leaves cointain secondary metabolites alkaloids,
glycosides, flavonoids, tannins, saponin and steroids. From the result obtained
ethyl acetate extract of the binahong leaves dose of 400 mg/kg body weight give
the effect of a decrease in cholesterol levels is not significantly different (p ≤ 0.05)
from simvastatin suspension, whereas a doses of 100 mg/kg body weight and 200
mg/kg body weight lower cholesterol is not better than simvastatin. It was
concluded that the ethyl acetate extract of binahong leaves dose of 400 mg/kg
body weight is effective dose to decrease serum cholesterol level in
hypercholesterolemia guinea pigs.
Keywords : (Anredera cordifolia (Ten.) Steenis), Characterization of Simplex,
Hypercholesterolemia, Phytochemical screening.
viii
Universitas Sumatera Utara
Cordifolia (Ten.) Steenis) pada Serum Darah Marmot
ABSTRAK
Binahong (Anredera cordifolia (Ten.) Steenis) merupakan tumbuhan
menjalar yang memiliki banyak manfaat untuk mengobati berbagai penyakit,
seperti menurunkan kolesterol. Kolesterol merupakan faktor pembentukan
aterosklerosis arteri-arteri vital, yang menimbulkan penyakit pembuluh darah
perifer, koroner, dan serebrovaskular. Tujuan penelitian ini adalah mengetahui
pengaruh pemberian ekstrak etil asetat daun binahong terhadap kadar kolesterol
darah marmot yang dibuat hiperkolesterolemia, dan membandingkan efek
penurunan kolesterol dari ekstrak etil asetat daun binahong dengan obat
simvastatin.
Sampel daun binahong dikarakterisasi dan dilakukan skrining fitokimia,
sebelum diuji sebagai penurunan kolesterol marmot. Marmot hiperkolesterolemia
dibuat dengan memberikan makanan induksi kuning telur (dosis 1% bb) dicampur
lemak kambing 15 g selama 14 hari. Suspensi ekstrak etil asetat daun binahong
diberikan per oral dengan dosis 100 mg/kgbb, 200 mg/kgbb, dan 400 mg/kgbb,
sebagai kontrol positif adalah simvastatin dan CMC Na sebagai kontrol pelarut
selama 7 hari pada marmot yang mengalami hiperkolesterolemia.
Hasil pemeriksaan karakterisasi simplisia daun binahong yaitu kadar air
7,32%, kadar sari larut air 26,16%, kadar sari larut etanol 25,86%, kadar abu total
6,35%, dan kadar abu tidak larut asam 0,58%. Simplisia daun binahong
mengandung senyawa metabolit sekunder alkaloid, glikosida, flavonoid, tannin,
saponin dan steroid. Hasil penelitian menunjukkan ekstrak etil asetat daun
binahong 400 mg/kgbb memberikan efek penurunan kadar kolesterol yang tidak
berbeda nyata (p ≤ 0,05) dengan pemberian simvastatin, dibandingkan dosis 100
mg/kgbb dan 200 mg/kgbb menurunkan kolesterol tidak lebih baik dari
simvastatin. Disimpulkan bahwa pemberian ekstrak etil asetat daun binahong
dosis 400 mg/kgbb merupakan dosis efektif untuk penurunan kadar kolesterol
serum pada marmot hiperkolesterolemia.
Kata
Kunci:
(Anredera cordifolia (Ten.) Steenis),
Karakterisisasi simplisia, Skrining fitokimia,
Hiperkolesterolemia
vii
Universitas Sumatera Utara
Anti-Hypercholesterolemia of Ethyl Acetate Extract of the Binahong Leaves
(Anredera cordifolia (Ten.) Steenis) in Blood Serum Guinea Pig
ABSTRACT
Binahong (Anredera cordifolia (Ten.) Steenis) are vines that have many
benefits for treating various diseases, such as to lower cholesterol. Cholesterol is a
factor of the formation of atherosclerosis vital arteries, causing peripheral vascular
disease, coronary and cerebrovascular. The purpose of this research was to
determine the effect of ethyl acetate extract of the binahong leaves on blood
cholesterol levels guinea pig made hypercholesterolemia, and comparing the
cholesterol lowering effect of ethyl acetate extract of the binahong leaves with
simvastatin drug.
Sample of binahong leaf were characterized and phytochemical screening
done before in trials as a cholesterol-lowering effect of a guinea pig. Guinea pig
hypercholesterolemia made with food induced yolk (dose 1% body weight) mixed
with goat fat 15 g for 14 days. Suspension of ethyl acetate extract of the leaves
binahong given orally at a dose of 100 mg/kg body weight, 200 mg/kg body
weight, 400 mg/kg body weight, a positive control is simvastatin and CMC Na as
a solvent control For 7 days in guinea pig who have hypercholesterolemia.
The result of simplex characterization of binahong leaves is the water
content value 7.32%, the water soluble extract value 26.16%, the ethanol soluble
extract value 25.86%, the total ash 6.35%, and the ash is not soluble in acid
0.58%. Simplex of binahong leaves cointain secondary metabolites alkaloids,
glycosides, flavonoids, tannins, saponin and steroids. From the result obtained
ethyl acetate extract of the binahong leaves dose of 400 mg/kg body weight give
the effect of a decrease in cholesterol levels is not significantly different (p ≤ 0.05)
from simvastatin suspension, whereas a doses of 100 mg/kg body weight and 200
mg/kg body weight lower cholesterol is not better than simvastatin. It was
concluded that the ethyl acetate extract of binahong leaves dose of 400 mg/kg
body weight is effective dose to decrease serum cholesterol level in
hypercholesterolemia guinea pigs.
Keywords : (Anredera cordifolia (Ten.) Steenis), Characterization of Simplex,
Hypercholesterolemia, Phytochemical screening.
viii
Universitas Sumatera Utara