Uji Aktivitas Antiinfsi Ekstrak Etanol Kayu Siwak (Salvadora persica Wall) Terhadap Tikus Putih yang Diinduksi λ-Karagenan
Lampiran 1. Hasil identifikasi sampel
Universitas Sumatera Utara
Lampiran 2. Gambar tumbuhan siwak dan serbuk simplisia
www.wahidaindonesia.wordpress.com
www.fedipedia.org
a.
tumbuhan siwak
b. kayu siwak
c. simplisia kayu siwak
d. serbuk kayu siwak
Lampiran 3. Hasil mikroskopik serbuk simplisia kayu siwak
Universitas Sumatera Utara
1
2
3
4
5
Keterangan:
1. Serat kayu
2. Kristal Ca-oksalat bentuk prisma
3. Fragmen trakea
4. Sel parenkim
5. Jari-jari empulur
Lampiran 4. Perhitungan Hasil Karakterisasi Simplisia
Universitas Sumatera Utara
1. Penetapan Kadar Air Simplisia Kayu Siwak
Kadar Air =
Sampel I =
Volume air (ml)
berat sampel (g)
x 100%
Volume 0
= 0,9 ml
Volume I
= 1,25 ml
Berat sampel = 5,017 g
Kadar air
=
0,35 ml
5,017 g
x 100%
= 6,97% b/v
Sampel II =
Volume I
= 1,25 ml
Volume II
= 2,05 ml
Berat sampel = 5,032 g
Kadar air
=
0,8 ml
5,032 g
x 100%
= 7,35% b/v
Sampel III =
Volume II
= 2,05 ml
Volume III
= 2,35 ml
Berat sampel = 5,014
Kadar air
=
0,3 ml
5,014 g
x 100%
= 5,98% b/v
Kadar air rata-rata =
6,77%+7,35%+5,98%
3
= 5,77% b/v
Lampiran 4. lanjutan
Universitas Sumatera Utara
2. Penetapan Kadar Sari Larut Air
Kadar Sari Larut Air =
Sampel I
berat sari
berat sampel
x
100
20
x 100%
Berat sampel = 5,014 g
Berat sari
= 0,220 g
Kadar sari
=
0,220 g
5,014 g
x
100
20
x 100%
= 21,94%
Sampel II
Berat sampel = 5,006 g
Berat sari
= 0,249 g
Kadar sari
=
0,249 g
5,006 g
x
100
20
x 100%
= 24,87%
Sampel III
Berat sampel = 5,023 g
Berat sari
= 0,219 g
Kadar sari
=
0,219 g
5,023 g
x
100
20
x 100%
= 21,80%
Kadar sari larut air rata-rata =
21,94%+24,87%+21,80%
3
= 22,87%
Lampiran 4. lanjutan
Universitas Sumatera Utara
3. Penetapan Kadar Sari Larut Etanol
Kadar Sari Larut Etanol =
Sampel I
berat sari
berat sampel
x
100
x 100%
20
Berat sampel = 5,002 g
Berat sari
= 0,933 g
Kadar sari
=
0,993 g
x
5,002 g
100
20
x 100%
= 93,26%
Sampel II
Sampel III
Berat sampel = 5,016 g
Berat sari
= 0,124 g
Kadar sari
=
0,124 g
5,016 g
= 12,36%
x
100
20
x 100%
Berat sampel = 5,010 g
Berat sari
= 0,128 g
Kadar sari
=
Kadar sari larut etanol rata-rata =
0,128 g
5,010 g
= 12,77%
x
100
20
x 100%
93,26% + 12,36% + 12,77%
3
= 39,46%
Lampiran 4. Lanjutan
Universitas Sumatera Utara
4. Penetapan Kadar Abu Total
Kadar Abu Total =
berat abu
berat sampel
Sampel I
Sampel II
Sampel III
x 100%
Berat sampel = 2,003 g
Berat abu
= 0,13 g
Kadar abu
=
0,13 g
2,003
= 6,49%
x 100%
Berat sampel = 2,007 g
Berat abu
= 0,16 g
Kadar abu
=
0,16 g
2,007 g
= 7,97%
x 100%
Berat sampel = 2,014 g
Berat abu
= 0,20 g
Kadar abu
=
0,20 g
2,014 g
x 100%
= 9,93%
Kadar abu total rata-rata
=
6,14%+7,97%+9,93%
3
= 8,13%
Lampiran 4. Lanjutan
Universitas Sumatera Utara
5. Penentuan Kadar Abu Tidak Larut Asam
Kadar Abu Tidak Larut Asam =
Sampel I
berat abu
berat sampel
x 100%
Berat sampel = 2,003 g
Berat abu
= 0,0086 g
Kadar abu
=
0,0086 g
2,003
x 100%
= 0,42%
Sampel II
Berat sampel = 2,007 g
Berat abu
= 0,0054 g
Kadar abu
=
0,0054 g
2,007 g
x 100%
= 0,26%
Sampel III
Berat sampel = 2,014 g
Berat abu
= 0,0062 g
Kadar abu
=
0,0062 g
2,014 g
x 100%
= 0,30%
Kadar abu tidak larut asam rata-rata =
0,42%+0,26%+0,305
3
= 0,33%
Lampiran 5. Konversi dosis.
Universitas Sumatera Utara
Tabel konversi dosis antara jenis hewan dengan manusia (Darmono, 2011).
Mencit
20g
Tikus
200g
Marmut
400 g
Kelinci
1,2 kg
Kera
4 kg
Anjing
12 kg
Manusia
70 kg
Mencit
Tikus
Marmut
Kelinci
Kera
Anjing
Manusia
20 g
200 g
400 g
1,2 kg
4 kg
12 kg
70 kg
1,0
7,0
12,25
27,8
64,1
124,2
387,9
0,14
1,0
1,74
3,9
9,2
17,8
56,0
0,08
0,57
1,0
2,25
5,2
10,2
31,5
0,04
0,25
0,44
1,0
2,4
4,5
14,2
0,016
0,11
0,19
0,42
1,0
1,9
6,1
0,008
0,06
0,10
0,22
0,52
1,0
3,1
0,0026
0,018
0,031
0,07
0,16
0,32
1,0
Lampiran 6. Contoh perhitungan dosis
Universitas Sumatera Utara
-
Kontrol positif (natrium diklofenak)
Pemakaian untuk manusia 25-50 mg (Tjay, T.H., 2007)
Konversi dari manusia (70 kg) untuk tikus 200 g = 0,018
25 mg x 0,018 = 0,45 mg/200 g bb
= 0,45 mg/
200
1000
= 0,45 mg x
1000
200
kg
= 2,25 mg/kg bb ~ 2,50 mg/kg bb
Diketahui: Berat Tikus
Dosis
=150 g
= 2,50 mg/kg bb
Maka natrium diklofenak ditimbang sebanyak 2,50 mg dilarutkan dalam 10
ml suspensi Na CMC 0,5 %.
Volume pemberian 1% bb tikus, maka volume suspensi natrium diklofenak
yang diberikan untuk tikus 150 g adalah
-
1
100
x 150 g = 1,5 ml
Ekstrak Etanol Kayu Siwak 200; 400; 600 mg/kg bb
Diketahui: Berat Tikus
= 150 g
Maka EEKS ditimbang masing-masing sebanyak 200; 400; 600 mg, kemudian
tiap dosis dilarutkan dalam 10 ml suspensi Na CMC 0,5 %.
Volume pemberian 1% bb tikus, maka volume suspensi EEKS yang diberikan
untuk tikus 150 g adalah
1
100
x 150 g = 1,5 ml
Universitas Sumatera Utara
Lampiran 7. Gambar alat, tikus dan telapak kaki tikus
Pletismometer digital
Universitas Sumatera Utara
Lampiran 7. Lanjutan
Tikus putih jantan
Telapak kaki tikus sebelum diinduksi karagenan
Telapak kaki tikus setelah diinduksi karagenan
Universitas Sumatera Utara
Lampiran 8. Contoh perhitungan persen radang dan persen inhibisi radang
1. Persen Radang
Persen Radang =
Vt-V0
V0
x 100%
Dimana: Vt = volume radang setelah waktu t
Vo = volume awal kaki tikus
Misalnya: Ekstrak etanol kayu siwak 200 mg/kg bb pada menit ke-30
Diketahui: Vt = 04.09
Vo = 03.21
Persen Radang =
04.09-03.29
03.29
x 100%
= 27,41%
2. Persen Inhibisi Radang
Persen Inhibisi Radang =
a-b
a
x 100%
Dimana: a = Persen radang rata-rata kelompok kontrol
b = Persen radang rata-rata kelompok perlakuan yang mendapat bahan
uji atau obat pembanding
Misalnya: Ekstrak etanol kayu siwak dosis 200 mg/kg bb pada menit ke 30
Diketahui: a = 30,41%,
b = 27,91%
Persen Inhibisi Radang =
30.41-27.91
30.41
x 100% = 8,22%
Universitas Sumatera Utara
Lampiran 9. Hasil pengukuran volume telapak kaki tikus, persen radang dan
persen inhibisi radang
Kelompok
Kontrol CMC 0,5 %
Natrium diklofenak
2,50 mg/kg bb
EEKS 200 mg/kg bb
EEKS 400 mg/kg bb
EEKS 600 mg/kg bb
Tikus
V0
60
03.41
90
03.63
Volume telapak kaki tikus pada menit ke-n
120
150
180
210
240
03.87
03.98
04.03
04.03 03.77
1
03.01
30
03.40
2
03.31
03.73
03.84
03.95
04.08
04.14
04.15
04.13
04.10
3
03.07
03.46
03.54
03.72
03.80
03.85
03.86
03.82
03.70
4
5
03.26
03.39
03.47
03.74
03.57
03.90
03.82
04.09
04.25
04.09
04.30
04.36
04.56
04.40
04.58
04.26
04.46
04.24
1
2
3
03.75
03.61
04.03
04.95
04.55
05.10
05.20
04.97
05.45
05.21
05.03
05.54
05.28
05.10
05.59
05.40
05.13
05.61
05.53
05.28
05.68
05.48
05.35
05.79
05.62
05.43
05.88
4
5
03.17
03.34
04.35
04.35
04.55
04.70
04.70
04.78
04.79
04.93
04.90
05.05
05.03
05.10
05.13
05.28
05.25
05.30
1
2
3
03.21
03.20
03.63
04.09
04.12
03.91
04.17
04.04
03.91
04.26
04.14
04.25
04.19
04.29
04.31
04.24
04.30
04.42
04.49
04.45
04.47
04.67
04.59
04.53
04.83
04.67
04.76
4
5
03.75
03.30
04.74
04.75
04.82
04.82
04.98
04.99
04.95
05.05
05.11
05.20
05.29
05.32
05.35
05.47
05.37
05.51
1
2
03.30
03.61
03.96
04.27
04.17
04.32
04.24
04.41
04.39
04.52
04.42
04.67
04.65
04.89
04.70
04.91
04.56
05.06
3
4
5
03.39
03.44
03.07
04.02
04.08
03.69
04.09
04.27
03.65
04.23
04.33
03.79
04.25
04.56
03.93
04.37
04.72
04.01
04.45
04.73
04.15
04.57
04.93
04.21
04.73
04.87
04.32
1
03.52
03.98
04.10
04.18
04.45
04.47
04.56
04.58
04.50
2
3
4
5
03.28
03.32
03.86
03.85
03.80
03.85
04.37
04.40
03.92
04.02
04.53
04.62
04.00
04.17
04.75
04.80
04.09
04.30
04.97
04.93
04.24
04.40
05.07
05.06
04.30
04.45
05.20
05.10
04.34
04.43
05.26
05.17
04.27
04.38
04.92
04.95
Universitas Sumatera Utara
Lampiran 9. Lanjutan
Kel
omp
ok
Kon
trol
CM
C
0,5
%
Tikus
1
2
3
4
5
Ratarata
SD
Natr
ium
dikl
ofen
ak
2,50
mg/
kg
bb
1
2
3
4
5
Ratarata
SD
EE
KS
200
mg/
kg
bb
1
2
3
4
5
Ratarata
SD
Persen Radang pada Menit ke-n (%)
12 15 18 21 24 27
0
0
0
0
0
0
40, 44, 47, 46, 49, 48,
80 00 47 13 87 80
41, 42, 46, 48, 50, 52,
27 11 26 20 42 08
38, 38, 40, 43, 45, 48,
71 21 94 67 91 39
51, 54, 58, 61, 65, 68,
10 57 68 83 62 77
47, 51, 52, 58, 58, 57,
60 20 69 08 68 19
43, 46, 49, 51, 54, 55,
90 22 21 58 10 05
±5. ±6. ±6. ±7. ±7. ±8.
22 43 74 92 94 44
28, 32, 33, 33, 25, 20,
57 23 89 89 25 27
23, 25, 25, 24, 23, 21,
26 08 38 77 87 15
23, 25, 25, 24, 20, 18,
78 41 73 43 52 89
30, 31, 39, 40, 36, 33,
37 90 88 49 81 74
20, 28, 29, 25, 25, 21,
65 61 79 66 07 53
30
60
90
32,
00
26,
04
26,
55
37,
22
30,
24
30,
41
±4.
55
12,
96
12,
69
12,
70
6,4
4
10,
34
38,
67
37,
67
35,
24
43,
53
40,
72
39,
17
±3.
14
13,
29
16,
01
15,
31
9,5
1
15,
04
38,
93
39,
34
37,
47
48,
26
43,
11
41,
42
±4.
35
20,
60
19,
34
21,
17
17,
18
20,
65
11,
03
13,
83
19,
79
25,
33
28,
65
30,
93
29,
85
26,
30
±2.
78
27,
41
28,
75
26,
13
30,
58
26,
67
27,
91
±1.
79
±2.
62
29,
91
26,
25
26,
13
32,
78
28,
53
28,
72
±2.
77
±1.
61
32,
71
29,
38
37,
10
32,
78
28,
53
32,
10
±3.
39
±4.
02
30,
53
34,
06
39,
03
36,
36
34,
67
34,
93
±3.
12
±3.
41
32,
09
34,
38
42,
58
40,
77
38,
67
37,
70
±4.
38
±6.
08
39,
88
39,
06
44,
19
45,
73
41,
87
42,
15
±2.
82
±7.
11
45,
48
43,
44
46,
13
47,
38
45,
87
45,
66
±1.
43
±6.
17
50,
47
45,
94
53,
55
47,
93
46,
93
48,
96
±3.
07
30
0
52,
53
51,
52
52,
11
61,
83
55,
39
54,
68
±4.
27
16,
94
20,
54
17,
59
30,
67
15,
93
36
0
47,4 49,
7
60
52,9 48,
1
48
45,1 47,
6
39
56,7 52,
8
37
56,5 51,
9
20
51,7 49,
8
81
±5.2 ±2.
9
01
11,
9,63
30
13,2 9,0
9
6
12,7 9,7
0
7
59,5 25,
1
15
10,3 6,1
2
9
23,
12
20,
33
21,0
9
12,
29
±6.
03
54,
52
51,
88
54,
52
48,
76
51,
73
52,
28
±2.
39
±6.
03
52,
34
50,
94
54,
19
46,
28
44,
27
49,
60
±4.
18
±21.
53
42,3
7
47,1
9
53,2
3
44,0
8
43,7
3
46,1
2
±4.3
5
±7.
42
35,
51
42,
81
51,
61
41,
32
38,
93
42,
04
±6.
02
330
Universitas Sumatera Utara
Lampiran 9. Lanjutan
EE
KS
400
mg/
kg
bb
20,
00
18,
28
18,
58
18,
60
20,
20
26,
36
19,
67
20,
65
24,
13
18,
89
28,
48
22,
16
24,
78
25,
87
23,
45
33,
03
25,
21
25,
37
32,
26
28,
01
33,
94
29,
36
28,
91
37,
21
30,
62
40,
91
35,
46
31,
27
37,
50
35,
18
42,
42
36,
01
34,
81
43,
31
37,
13
40,
91
40,
17
39,
53
41,
57
40,
72
37,
27
35,
46
43,
07
39,
53
38,
11
33,
64
29,
36
38,
64
33,
14
31,
27
28,
18
26,
04
27,
14
30,
52
28,
66
23,
94
24,
10
23,
60
26,
16
21,
50
19,
13
21,
94
24,
95
28,
78
32,
01
36,
06
38,
74
40,
58
38,
69
33,
21
28,
11
23,
86
±0.
90
13,
07
15,
85
15,
96
13,
21
14,
29
±3.
18
16,
48
19,
51
21,
08
17,
36
20,
00
±2.
42
18,
75
21,
95
25,
60
23,
06
24,
68
±3.
71
26,
42
24,
70
29,
52
28,
76
28,
05
±3.
51
26,
99
29,
27
32,
53
31,
35
31,
45
±3.
52
29,
55
31,
10
34,
04
34,
72
32,
47
±3.
87
30,
11
32,
32
33,
43
36,
27
34,
29
±0.
77
27,
84
30,
18
31,
93
27,
46
28,
57
±2.
86
23,
58
25,
91
30,
42
28,
24
26,
75
±3.
47
21,
88
25,
61
29,
22
25,
13
22,
86
±1.
69
17,
33
21,
65
26,
81
22,
02
20,
26
±1.
66
14,
20
16,
16
21,
99
19,
95
18,
18
Rat
arata
14,
48
18,
89
22,
81
27,
49
30,
31
32,
38
33,
28
29,
20
26,
98
24,
94
21,
61
18,
10
SD
±1.
39
±1.
91
±2.
67
±1.
93
±2.
20
±2.
11
±2.
29
±1.
85
±2.
56
±2.
85
±3.
44
±3.
06
30
60
Persen Inhibisi Radang pada Menit ke-n (%)
90
120 150 180 210 240 270 300
330
360
63,7
3
64,6
9
52,2
2
42,3
0
38,0
1
37,1
5
42,1
3
51,3
9
58,0
0
62,8
2
59,2
7
75,3
3
8,22
26,6
8
22,5
0
20,4
3
18,4
3
14,3
5
11,4
8
9,50
5,03
9,29
10,9
3
15,6
0
37,0
9
43,9
8
39,7
6
34,4
4
30,7
4
26,7
2
24,8
9
24,9
9
29,7
2
39,2
6
45,7
1
52,1
0
52,3
8
48,9
6
44,9
3
37,3
8
34,4
2
34,2
0
35,4
8
46,0
2
50,9
9
54,3
9
58,2
7
63,6
6
1
2
3
4
5
Rat
arata
SD
EE
KS
600
mg/
kg
bb
1
2
3
4
5
Kelomp
ok
Natrium
diklofena
k
2,50
mg/kg bb
EEKS
200
mg/kg bb
EEKS
400
mg/kg bb
EEKS
600
mg/kg bb
Universitas Sumatera Utara
Lampiran 10. Hasil uji statistik persen radang dengan SPSS
Menit ke-30
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Std.
Deviation Error
5
30.4100
4.55328
2.03629 24.7564 36.0636 26.04
37.22
Na
diklofenak
2,50 5
mg/kg bb
EEKS 200 mg/kg bb
5
11.0260
2.77550
1.24124 7.5798
12.96
27.9080
1.78819
.79970
25.6877 30.1283 26.13
30.58
EEKS 400 mg/kg bb
5
19.1320
.89550
.40048
18.0201 20.2439 18.28
20.20
EEKS 600 mg/kg bb
5
14.4760
1.38783
.62066
12.7528 16.1992 13.07
15.96
Total
25
20.5904
8.01628
1.60326 17.2814 23.8994 6.44
Sum of Squares
df
Mean Square
F
Sig.
1404.812
137.446
1542.257
4
20
24
351.203
6.872
51.104
.000
Na CMC 0,5%
Lower
Bound
Upper
Bound
Minimum Maximum
14.4722 6.44
37.22
ANOVA
% radang kaki tikus
Between Groups
Within Groups
Total
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
Na diklofenak 2,50 mg/kg bb
EEKS 600 mg/kg bb
EEKS 400 mg/kg bb
EEKS 200 mg/kg bb
Na CMC 0,5%
Sig.
N
1
5
5
5
5
5
2
3
11.0260
14.4760
19.1320
.051
1.000
27.9080
30.4100
.147
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-60
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Std.
Deviation
Mean
Lower
Bound
Std. Error
Upper
Bound Minimum Maximum
Na CMC 0,5%
5 39.1660
3.13675
1.40280
35.2712 43.0608
35.24
43.53
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 13.8320
2.61538
1.16963
10.5846 17.0794
9.51
16.01
5 28.7200
2.77240
1.23985
25.2776 32.1624
26.13
32.78
EEKS 400 mg/kg bb
5 21.9400
3.18009
1.42218
17.9914 25.8886
18.89
26.36
EEKS 600 mg/kg bb
5 18.8860
1.90798
.85327
16.5169 21.2551
16.48
21.08
Total
25 24.5088
9.30134
1.86027
20.6694 28.3482
9.51
43.53
ANOVA
% radang kaki tikus
Sum of Squares
Between Groups
Within Groups
Total
df
1923.882
152.476
2076.358
Mean Square
4
20
24
F
480.970
7.624
Sig.
63.088
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
Na diklofenak 2,50 mg/kg bb
EEKS 600 mg/kg bb
EEKS 400 mg/kg bb
EEKS 200 mg/kg bb
Na CMC 0,5%
Sig.
N
1
5
5
5
5
5
2
3
4
13.8320
18.8860
21.9400
28.7200
1.000
.096
1.000
39.1660
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-90
Descriptives
% radang kaki tikus
95% Confidence Interval
for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5 41.4220
4.35232 1.94642
36.0179
46.8261
37.47
48.26
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 19.7880
1.60582
.71815
17.7941
21.7819
17.18
21.17
5 32.1000
3.39035 1.51621
27.8903
36.3097
28.53
37.10
EEKS 400 mg/kg bb
5 24.9480
2.41714 1.08098
21.9467
27.9493
22.16
28.48
EEKS 600 mg/kg bb
5 22.8080
2.67239 1.19513
19.4898
26.1262
18.75
25.60
Total
25 28.2132
8.38204 1.67641
24.7533
31.6731
17.18
48.26
Sum of Squares
df
ANOVA
% radang kaki tikus
Mean Square
Between Groups
Within Groups
1502.207
184.000
4
20
Total
1686.207
24
F
375.552
9.200
Sig.
40.821
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
Na diklofenak 2,50 mg/kg bb
EEKS 600 mg/kg bb
EEKS 400 mg/kg bb
EEKS 200 mg/kg bb
Na CMC 0,5%
Sig.
5
5
5
5
5
19.7880
22.8080
2
3
4
22.8080
24.9480
32.1000
.131
.278
1.000
41.4220
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-120
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5
43.8960
5.21998 2.33445
37.4145
50.3775
38.71
51.10
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5
25.3260
4.01525 1.79567
20.3404
30.3116
20.65
30.37
5
34.9300
3.12408 1.39713
31.0509
38.8091
30.53
39.03
EEKS 400 mg/kg bb
5
28.7760
3.71277 1.66040
24.1660
33.3860
25.21
33.03
EEKS 600 mg/kg bb
5
27.4900
1.93497
.86534
25.0874
29.8926
24.70
29.52
Total
25
32.0836
7.66307 1.53261
28.9204
35.2468
20.65
51.10
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
1126.707
282.636
4
20
Total
1409.343
24
F
281.677
14.132
19.932
Sig.
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
Na diklofenak 2,50 mg/kg bb
5
25.3260
EEKS 600 mg/kg bb
5
27.4900
EEKS 400 mg/kg bb
5
28.7760
EEKS 200 mg/kg bb
5
Na CMC 0,5%
5
Sig.
3
34.9300
43.8960
.184
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-150
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5 46.2180
6.43202 2.87649
38.2316
54.2044
39.21
54.57
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 28.6460
3.41405 1.52681
24.4069
32.8851
25.08
32.23
5 37.6980
4.37820 1.95799
32.2617
43.1343
32.09
42.58
EEKS 400 mg/kg bb
5 32.0080
3.51175 1.57050
27.6476
36.3684
28.91
37.21
EEKS 600 mg/kg bb
5 30.3180
2.20303
.98522
27.5826
33.0534
26.99
32.53
Total
25 34.9776
7.58111 1.51622
31.8483
38.1069
25.08
54.57
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
1021.834
357.524
4
20
Total
1379.357
24
F
255.458
17.876
14.290
Sig.
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
Na diklofenak 2,50 mg/kg bb
5
28.6460
EEKS 600 mg/kg bb
5
30.3180
EEKS 400 mg/kg bb
5
32.0080
EEKS 200 mg/kg bb
5
Na CMC 0,5%
5
Sig.
3
37.6980
46.2180
.248
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-180
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5 49.2080
6.74378 3.01591
40.8345
57.5815
40.94
58.68
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 30.9340
6.08289 2.72035
23.3811
38.4869
25.38
39.88
5 42.1460
2.81768 1.26011
38.6474
45.6446
39.06
45.73
EEKS 400 mg/kg bb
5 36.0640
3.52401 1.57598
31.6884
40.4396
31.27
40.91
EEKS 600 mg/kg bb
5 32.3760
2.11462
.94569
29.7503
35.0017
29.55
34.72
Total
25 38.1456
8.08620 1.61724
34.8078
41.4834
25.38
58.68
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
1140.042
429.239
4
20
Total
1569.281
24
F
285.011
21.462
13.280
Sig.
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
Na diklofenak 2,50 mg/kg bb
5
30.9340
EEKS 600 mg/kg bb
5
32.3760
EEKS 400 mg/kg bb
5
36.0640
EEKS 200 mg/kg bb
5
Na CMC 0,5%
5
Sig.
3
36.0640
42.1460
49.2080
.112
.051
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-210
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5 51.5820
7.92161 3.54265
41.7460
61.4180
43.67
61.83
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 29.8480
7.11108 3.18017
21.0184
38.6776
24.43
40.49
5 45.6600
1.43040
.63970
43.8839
47.4361
43.44
47.38
EEKS 400 mg/kg bb
5 38.7360
3.87031 1.73085
33.9304
43.5416
34.81
43.31
EEKS 600 mg/kg bb
5 33.2840
2.28873 1.02355
30.4422
36.1258
30.11
36.27
Total
25 39.8220
9.40137 1.88027
35.9413
43.7027
24.43
61.83
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
1578.927
542.332
4
20
Total
2121.259
24
F
394.732
27.117
Sig.
14.557
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
3
Na diklofenak 2,50 mg/kg bb
5
29.8480
EEKS 600 mg/kg bb
5
33.2840
EEKS 400 mg/kg bb
5
EEKS 200 mg/kg bb
5
45.6600
Na CMC 0,5%
5
51.5820
Sig.
33.2840
38.7360
.309
.113
.087
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-240
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound Minimum Maximum
Na CMC 0,5%
5 54.1000
7.94037 3.55104
44.2407 63.9593
45.91
65.62
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 26.3040
6.17229 2.76033
18.6401 33.9679
20.52
36.81
5 48.9640
3.06799 1.37205
45.1546 52.7734
45.94
53.55
EEKS 400 mg/kg bb
5 40.5800
.77123
.34491
39.6224 41.5376
39.53
41.57
EEKS 600 mg/kg bb
5 29.1960
1.84982
.82726
26.8991 31.4929
27.46
31.93
25 39.8288 11.86136 2.37227
34.9327 44.7249
20.52
65.62
Total
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
2918.300
458.304
4
20
Total
3376.604
24
F
729.575
22.915
Sig.
31.838
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
3
Na diklofenak 2,50 mg/kg bb
5
26.3040
EEKS 600 mg/kg bb
5
29.1960
EEKS 400 mg/kg bb
5
EEKS 200 mg/kg bb
5
48.9640
Na CMC 0,5%
5
54.1000
Sig.
40.5800
.351
1.000
.105
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-270
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5 55.0460
8.44181 3.77529
44.5641
65.5279
48.39
68.77
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 23.1160
6.02513 2.69452
15.6348
30.5972
18.89
33.74
5 52.2820
2.39207 1.06977
49.3118
55.2522
48.76
54.52
EEKS 400 mg/kg bb
5 38.6880
2.85813 1.27820
35.1392
42.2368
35.46
43.07
EEKS 600 mg/kg bb
5 26.9800
2.55827 1.14409
23.8035
30.1565
23.58
30.42
25 39.2224 13.94124 2.78825
33.4677
44.9771
18.89
68.77
Total
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
4152.588
512.008
4
20
Total
4664.596
24
F
1038.147
25.600
Sig.
40.552
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
3
Na diklofenak 2,50 mg/kg bb
5
23.1160
EEKS 600 mg/kg bb
5
26.9800
EEKS 400 mg/kg bb
5
EEKS 200 mg/kg bb
5
52.2820
Na CMC 0,5%
5
55.0460
Sig.
38.6880
.241
1.000
.398
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-300
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5
54.6760
4.26730 1.90840
49.3774
59.9746
51.52
61.83
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5
20.3340
6.02742 2.69555
12.8500
27.8180
15.93
30.67
5
49.6040
4.17740 1.86819
44.4171
54.7909
44.27
54.19
EEKS 400 mg/kg bb
5
33.2100
3.47350 1.55340
28.8971
37.5229
29.36
38.64
EEKS 600 mg/kg bb
5
24.9400
2.85015 1.27463
21.4011
28.4789
21.88
29.22
Total
25
36.5528 14.30041 2.86008
30.6499
42.4557
15.93
61.83
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
4539.326
368.716
4
20
Total
4908.041
24
F
1134.831
18.436
Sig.
61.556
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
3
Na diklofenak 2,50 mg/kg bb
5
20.3340
EEKS 600 mg/kg bb
5
24.9400
EEKS 400 mg/kg bb
5
EEKS 200 mg/kg bb
5
49.6040
Na CMC 0,5%
5
54.6760
Sig.
33.2100
.105
1.000
.077
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-330
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
Lower
Bound
Upper
Bound
5.28709 2.36446
45.2172
58.3468
45.16
56.78
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 21.0900 21.53284 9.62978
-5.6466
47.8266
9.63
59.51
5 46.1200
4.34837 1.94465
40.7208
51.5192
42.37
53.23
EEKS 400 mg/kg bb
5 28.1280
1.68550
.75378
26.0352
30.2208
26.04
30.52
EEKS 600 mg/kg bb
5 21.6140
3.44059 1.53868
17.3419
25.8861
17.33
26.81
25 33.7468 16.05653 3.21131
27.1190
40.3746
9.63
59.51
N
Na CMC 0,5%
Total
Mean
5 51.7820
Std.
Deviation
Std.
Error
Minimum Maximum
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
4086.674
2100.814
4
20
Total
6187.488
24
F
1021.669
105.041
Sig.
9.726
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
Na diklofenak 2,50 mg/kg bb
5
21.0900
EEKS 600 mg/kg bb
5
21.6140
EEKS 400 mg/kg bb
5
28.1280
EEKS 200 mg/kg bb
5
46.1200
Na CMC 0,5%
5
51.7820
Sig.
.317
.393
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-360
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Std.
Deviation
Mean
Std.
Error
Lower
Bound
Upper
Bound
Minimum
Maximum
Na CMC 0,5%
5 49.8080
2.00967
.89875
47.3127
52.3033
47.39
52.37
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 12.2940
7.42214 3.31928
3.0782
21.5098
6.19
25.15
5 42.0360
6.02099 2.69267
34.5600
49.5120
35.51
51.61
EEKS 400 mg/kg bb
5 23.8600
1.65765
.74132
21.8018
25.9182
21.50
26.16
EEKS 600 mg/kg bb
5 18.0960
3.06327 1.36994
14.2924
21.8996
14.20
21.99
25 29.2188 15.22803 3.04561
22.9330
35.5046
6.19
52.37
Total
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
5135.390
430.043
4
20
Total
5565.433
24
F
1283.848
21.502
Sig.
59.708
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
Na diklofenak 2,50 mg/kg bb
5
12.2940
EEKS 600 mg/kg bb
5
18.0960
EEKS 400 mg/kg bb
5
EEKS 200 mg/kg bb
5
Na CMC 0,5%
5
Sig.
3
4
18.0960
23.8600
42.0360
49.8080
.062
.063
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 11. Perhitungan AUC
V1+V0
V2+V1
V12+V11
AUC = V0 �t1-t0� + V1 �t2-t1� + … … … . + V11 (t12 – t11)
=
3.40+3.01
3.01
3.87+3.63
(30 − 0) +
(120 − 90) +
3.63
4.03 +4.03
4.03
3.41+3.40
(60 − 30) +
3.40
3.98 + 3.87
(210 − 180) +
3.41
(150 − 120) +
3.87
3.77 + 4.03
4.03
3.63+3.41
(90 − 60) +
4.03 + 3.98
(240 − 210) +
(180 − 150) +
3.98
3.62 + 3.77
3.77
3.52 + 3.62
4.43 + 3.52
240) + 3.62 (300 − 270) + 3.52 (330 − 300) +
3.35 + 4.43
(270 −
(360 − 330)
Rata-rata = 68,89 + 60,09 + 61,94 + 61,98 + 60,85 + 60,38 + 60,00 + 58,06 +
58,80 + 59,17 + 67,76 +52,69 = 725,61
AUC
4.43
Na.diklofenak
2,50 mg/kg
bb
CMC Na
0,5%
EEKS 200
mg/kg bb
EEKS 400
mg/kg bb
EEKS 600
mg/kg bb
Tikus 1
Tikus 2
Tikus 3
Tikus 4
Tikus 5
725.61
663.11
723.13
728.38
722.21
733.45
732.95
731.69
734.45
733.41
730.32
731.85
737.99
725.48
736.08
729.15
732.46
730.03
729.62
729.54
719.50
721.97
726.42
725.88
723.44
Rata-rata
712.46
733.19
732.34
730.16
723.44
Kelompok
Universitas Sumatera Utara
Lampiran 11. Lanjutan
Descriptives
AUC
95% Confidence Interval for
Mean
N
Na.diklofenak
Mean
Std. Deviation Std. Error Lower Bound Upper Bound Minimum Maximum
5 712.4880
CMC Na 0,5 %
27.70694 12.39092
678.0853
746.8907
663.11
728.38
5 733.2440
1.02882
.46010
731.9665
734.5215
731.69
734.45
EEKS 200 mg/kg bb
5 732.3440
4.93179
2.20556
726.2204
738.4676
725.48
737.99
EEKS 400 mg/kg bb
5 730.1600
1.32316
.59173
728.5171
731.8029
729.15
732.46
EEKS 600 mg.kg bb
5 723.4420
2.85125
1.27512
719.9017
726.9823
719.50
726.42
Total
25 726.3356
14.00097
2.80019
720.5563
732.1149
663.11
737.99
Sum of Squares
df
ANOVA
AUC
Mean Square
Between Groups
1492.909
4
373.227
Within Groups
3211.744
20
160.587
Total
4704.654
24
F
2.324
Sig.
.092
AUC
Duncana
Subset for alpha = 0.05
kelompok perlakuan
N
1
2
Na.diklofenak
5
712.4880
EEKS 600 mg.kg bb
5
723.4420
EEKS 400 mg/kg bb
5
730.1600
EEKS 200 mg/kg bb
5
732.3440
CMC Na 0,5
5
733.2440
Sig.
.187
723.4420
.276
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Universitas Sumatera Utara
Lampiran 2. Gambar tumbuhan siwak dan serbuk simplisia
www.wahidaindonesia.wordpress.com
www.fedipedia.org
a.
tumbuhan siwak
b. kayu siwak
c. simplisia kayu siwak
d. serbuk kayu siwak
Lampiran 3. Hasil mikroskopik serbuk simplisia kayu siwak
Universitas Sumatera Utara
1
2
3
4
5
Keterangan:
1. Serat kayu
2. Kristal Ca-oksalat bentuk prisma
3. Fragmen trakea
4. Sel parenkim
5. Jari-jari empulur
Lampiran 4. Perhitungan Hasil Karakterisasi Simplisia
Universitas Sumatera Utara
1. Penetapan Kadar Air Simplisia Kayu Siwak
Kadar Air =
Sampel I =
Volume air (ml)
berat sampel (g)
x 100%
Volume 0
= 0,9 ml
Volume I
= 1,25 ml
Berat sampel = 5,017 g
Kadar air
=
0,35 ml
5,017 g
x 100%
= 6,97% b/v
Sampel II =
Volume I
= 1,25 ml
Volume II
= 2,05 ml
Berat sampel = 5,032 g
Kadar air
=
0,8 ml
5,032 g
x 100%
= 7,35% b/v
Sampel III =
Volume II
= 2,05 ml
Volume III
= 2,35 ml
Berat sampel = 5,014
Kadar air
=
0,3 ml
5,014 g
x 100%
= 5,98% b/v
Kadar air rata-rata =
6,77%+7,35%+5,98%
3
= 5,77% b/v
Lampiran 4. lanjutan
Universitas Sumatera Utara
2. Penetapan Kadar Sari Larut Air
Kadar Sari Larut Air =
Sampel I
berat sari
berat sampel
x
100
20
x 100%
Berat sampel = 5,014 g
Berat sari
= 0,220 g
Kadar sari
=
0,220 g
5,014 g
x
100
20
x 100%
= 21,94%
Sampel II
Berat sampel = 5,006 g
Berat sari
= 0,249 g
Kadar sari
=
0,249 g
5,006 g
x
100
20
x 100%
= 24,87%
Sampel III
Berat sampel = 5,023 g
Berat sari
= 0,219 g
Kadar sari
=
0,219 g
5,023 g
x
100
20
x 100%
= 21,80%
Kadar sari larut air rata-rata =
21,94%+24,87%+21,80%
3
= 22,87%
Lampiran 4. lanjutan
Universitas Sumatera Utara
3. Penetapan Kadar Sari Larut Etanol
Kadar Sari Larut Etanol =
Sampel I
berat sari
berat sampel
x
100
x 100%
20
Berat sampel = 5,002 g
Berat sari
= 0,933 g
Kadar sari
=
0,993 g
x
5,002 g
100
20
x 100%
= 93,26%
Sampel II
Sampel III
Berat sampel = 5,016 g
Berat sari
= 0,124 g
Kadar sari
=
0,124 g
5,016 g
= 12,36%
x
100
20
x 100%
Berat sampel = 5,010 g
Berat sari
= 0,128 g
Kadar sari
=
Kadar sari larut etanol rata-rata =
0,128 g
5,010 g
= 12,77%
x
100
20
x 100%
93,26% + 12,36% + 12,77%
3
= 39,46%
Lampiran 4. Lanjutan
Universitas Sumatera Utara
4. Penetapan Kadar Abu Total
Kadar Abu Total =
berat abu
berat sampel
Sampel I
Sampel II
Sampel III
x 100%
Berat sampel = 2,003 g
Berat abu
= 0,13 g
Kadar abu
=
0,13 g
2,003
= 6,49%
x 100%
Berat sampel = 2,007 g
Berat abu
= 0,16 g
Kadar abu
=
0,16 g
2,007 g
= 7,97%
x 100%
Berat sampel = 2,014 g
Berat abu
= 0,20 g
Kadar abu
=
0,20 g
2,014 g
x 100%
= 9,93%
Kadar abu total rata-rata
=
6,14%+7,97%+9,93%
3
= 8,13%
Lampiran 4. Lanjutan
Universitas Sumatera Utara
5. Penentuan Kadar Abu Tidak Larut Asam
Kadar Abu Tidak Larut Asam =
Sampel I
berat abu
berat sampel
x 100%
Berat sampel = 2,003 g
Berat abu
= 0,0086 g
Kadar abu
=
0,0086 g
2,003
x 100%
= 0,42%
Sampel II
Berat sampel = 2,007 g
Berat abu
= 0,0054 g
Kadar abu
=
0,0054 g
2,007 g
x 100%
= 0,26%
Sampel III
Berat sampel = 2,014 g
Berat abu
= 0,0062 g
Kadar abu
=
0,0062 g
2,014 g
x 100%
= 0,30%
Kadar abu tidak larut asam rata-rata =
0,42%+0,26%+0,305
3
= 0,33%
Lampiran 5. Konversi dosis.
Universitas Sumatera Utara
Tabel konversi dosis antara jenis hewan dengan manusia (Darmono, 2011).
Mencit
20g
Tikus
200g
Marmut
400 g
Kelinci
1,2 kg
Kera
4 kg
Anjing
12 kg
Manusia
70 kg
Mencit
Tikus
Marmut
Kelinci
Kera
Anjing
Manusia
20 g
200 g
400 g
1,2 kg
4 kg
12 kg
70 kg
1,0
7,0
12,25
27,8
64,1
124,2
387,9
0,14
1,0
1,74
3,9
9,2
17,8
56,0
0,08
0,57
1,0
2,25
5,2
10,2
31,5
0,04
0,25
0,44
1,0
2,4
4,5
14,2
0,016
0,11
0,19
0,42
1,0
1,9
6,1
0,008
0,06
0,10
0,22
0,52
1,0
3,1
0,0026
0,018
0,031
0,07
0,16
0,32
1,0
Lampiran 6. Contoh perhitungan dosis
Universitas Sumatera Utara
-
Kontrol positif (natrium diklofenak)
Pemakaian untuk manusia 25-50 mg (Tjay, T.H., 2007)
Konversi dari manusia (70 kg) untuk tikus 200 g = 0,018
25 mg x 0,018 = 0,45 mg/200 g bb
= 0,45 mg/
200
1000
= 0,45 mg x
1000
200
kg
= 2,25 mg/kg bb ~ 2,50 mg/kg bb
Diketahui: Berat Tikus
Dosis
=150 g
= 2,50 mg/kg bb
Maka natrium diklofenak ditimbang sebanyak 2,50 mg dilarutkan dalam 10
ml suspensi Na CMC 0,5 %.
Volume pemberian 1% bb tikus, maka volume suspensi natrium diklofenak
yang diberikan untuk tikus 150 g adalah
-
1
100
x 150 g = 1,5 ml
Ekstrak Etanol Kayu Siwak 200; 400; 600 mg/kg bb
Diketahui: Berat Tikus
= 150 g
Maka EEKS ditimbang masing-masing sebanyak 200; 400; 600 mg, kemudian
tiap dosis dilarutkan dalam 10 ml suspensi Na CMC 0,5 %.
Volume pemberian 1% bb tikus, maka volume suspensi EEKS yang diberikan
untuk tikus 150 g adalah
1
100
x 150 g = 1,5 ml
Universitas Sumatera Utara
Lampiran 7. Gambar alat, tikus dan telapak kaki tikus
Pletismometer digital
Universitas Sumatera Utara
Lampiran 7. Lanjutan
Tikus putih jantan
Telapak kaki tikus sebelum diinduksi karagenan
Telapak kaki tikus setelah diinduksi karagenan
Universitas Sumatera Utara
Lampiran 8. Contoh perhitungan persen radang dan persen inhibisi radang
1. Persen Radang
Persen Radang =
Vt-V0
V0
x 100%
Dimana: Vt = volume radang setelah waktu t
Vo = volume awal kaki tikus
Misalnya: Ekstrak etanol kayu siwak 200 mg/kg bb pada menit ke-30
Diketahui: Vt = 04.09
Vo = 03.21
Persen Radang =
04.09-03.29
03.29
x 100%
= 27,41%
2. Persen Inhibisi Radang
Persen Inhibisi Radang =
a-b
a
x 100%
Dimana: a = Persen radang rata-rata kelompok kontrol
b = Persen radang rata-rata kelompok perlakuan yang mendapat bahan
uji atau obat pembanding
Misalnya: Ekstrak etanol kayu siwak dosis 200 mg/kg bb pada menit ke 30
Diketahui: a = 30,41%,
b = 27,91%
Persen Inhibisi Radang =
30.41-27.91
30.41
x 100% = 8,22%
Universitas Sumatera Utara
Lampiran 9. Hasil pengukuran volume telapak kaki tikus, persen radang dan
persen inhibisi radang
Kelompok
Kontrol CMC 0,5 %
Natrium diklofenak
2,50 mg/kg bb
EEKS 200 mg/kg bb
EEKS 400 mg/kg bb
EEKS 600 mg/kg bb
Tikus
V0
60
03.41
90
03.63
Volume telapak kaki tikus pada menit ke-n
120
150
180
210
240
03.87
03.98
04.03
04.03 03.77
1
03.01
30
03.40
2
03.31
03.73
03.84
03.95
04.08
04.14
04.15
04.13
04.10
3
03.07
03.46
03.54
03.72
03.80
03.85
03.86
03.82
03.70
4
5
03.26
03.39
03.47
03.74
03.57
03.90
03.82
04.09
04.25
04.09
04.30
04.36
04.56
04.40
04.58
04.26
04.46
04.24
1
2
3
03.75
03.61
04.03
04.95
04.55
05.10
05.20
04.97
05.45
05.21
05.03
05.54
05.28
05.10
05.59
05.40
05.13
05.61
05.53
05.28
05.68
05.48
05.35
05.79
05.62
05.43
05.88
4
5
03.17
03.34
04.35
04.35
04.55
04.70
04.70
04.78
04.79
04.93
04.90
05.05
05.03
05.10
05.13
05.28
05.25
05.30
1
2
3
03.21
03.20
03.63
04.09
04.12
03.91
04.17
04.04
03.91
04.26
04.14
04.25
04.19
04.29
04.31
04.24
04.30
04.42
04.49
04.45
04.47
04.67
04.59
04.53
04.83
04.67
04.76
4
5
03.75
03.30
04.74
04.75
04.82
04.82
04.98
04.99
04.95
05.05
05.11
05.20
05.29
05.32
05.35
05.47
05.37
05.51
1
2
03.30
03.61
03.96
04.27
04.17
04.32
04.24
04.41
04.39
04.52
04.42
04.67
04.65
04.89
04.70
04.91
04.56
05.06
3
4
5
03.39
03.44
03.07
04.02
04.08
03.69
04.09
04.27
03.65
04.23
04.33
03.79
04.25
04.56
03.93
04.37
04.72
04.01
04.45
04.73
04.15
04.57
04.93
04.21
04.73
04.87
04.32
1
03.52
03.98
04.10
04.18
04.45
04.47
04.56
04.58
04.50
2
3
4
5
03.28
03.32
03.86
03.85
03.80
03.85
04.37
04.40
03.92
04.02
04.53
04.62
04.00
04.17
04.75
04.80
04.09
04.30
04.97
04.93
04.24
04.40
05.07
05.06
04.30
04.45
05.20
05.10
04.34
04.43
05.26
05.17
04.27
04.38
04.92
04.95
Universitas Sumatera Utara
Lampiran 9. Lanjutan
Kel
omp
ok
Kon
trol
CM
C
0,5
%
Tikus
1
2
3
4
5
Ratarata
SD
Natr
ium
dikl
ofen
ak
2,50
mg/
kg
bb
1
2
3
4
5
Ratarata
SD
EE
KS
200
mg/
kg
bb
1
2
3
4
5
Ratarata
SD
Persen Radang pada Menit ke-n (%)
12 15 18 21 24 27
0
0
0
0
0
0
40, 44, 47, 46, 49, 48,
80 00 47 13 87 80
41, 42, 46, 48, 50, 52,
27 11 26 20 42 08
38, 38, 40, 43, 45, 48,
71 21 94 67 91 39
51, 54, 58, 61, 65, 68,
10 57 68 83 62 77
47, 51, 52, 58, 58, 57,
60 20 69 08 68 19
43, 46, 49, 51, 54, 55,
90 22 21 58 10 05
±5. ±6. ±6. ±7. ±7. ±8.
22 43 74 92 94 44
28, 32, 33, 33, 25, 20,
57 23 89 89 25 27
23, 25, 25, 24, 23, 21,
26 08 38 77 87 15
23, 25, 25, 24, 20, 18,
78 41 73 43 52 89
30, 31, 39, 40, 36, 33,
37 90 88 49 81 74
20, 28, 29, 25, 25, 21,
65 61 79 66 07 53
30
60
90
32,
00
26,
04
26,
55
37,
22
30,
24
30,
41
±4.
55
12,
96
12,
69
12,
70
6,4
4
10,
34
38,
67
37,
67
35,
24
43,
53
40,
72
39,
17
±3.
14
13,
29
16,
01
15,
31
9,5
1
15,
04
38,
93
39,
34
37,
47
48,
26
43,
11
41,
42
±4.
35
20,
60
19,
34
21,
17
17,
18
20,
65
11,
03
13,
83
19,
79
25,
33
28,
65
30,
93
29,
85
26,
30
±2.
78
27,
41
28,
75
26,
13
30,
58
26,
67
27,
91
±1.
79
±2.
62
29,
91
26,
25
26,
13
32,
78
28,
53
28,
72
±2.
77
±1.
61
32,
71
29,
38
37,
10
32,
78
28,
53
32,
10
±3.
39
±4.
02
30,
53
34,
06
39,
03
36,
36
34,
67
34,
93
±3.
12
±3.
41
32,
09
34,
38
42,
58
40,
77
38,
67
37,
70
±4.
38
±6.
08
39,
88
39,
06
44,
19
45,
73
41,
87
42,
15
±2.
82
±7.
11
45,
48
43,
44
46,
13
47,
38
45,
87
45,
66
±1.
43
±6.
17
50,
47
45,
94
53,
55
47,
93
46,
93
48,
96
±3.
07
30
0
52,
53
51,
52
52,
11
61,
83
55,
39
54,
68
±4.
27
16,
94
20,
54
17,
59
30,
67
15,
93
36
0
47,4 49,
7
60
52,9 48,
1
48
45,1 47,
6
39
56,7 52,
8
37
56,5 51,
9
20
51,7 49,
8
81
±5.2 ±2.
9
01
11,
9,63
30
13,2 9,0
9
6
12,7 9,7
0
7
59,5 25,
1
15
10,3 6,1
2
9
23,
12
20,
33
21,0
9
12,
29
±6.
03
54,
52
51,
88
54,
52
48,
76
51,
73
52,
28
±2.
39
±6.
03
52,
34
50,
94
54,
19
46,
28
44,
27
49,
60
±4.
18
±21.
53
42,3
7
47,1
9
53,2
3
44,0
8
43,7
3
46,1
2
±4.3
5
±7.
42
35,
51
42,
81
51,
61
41,
32
38,
93
42,
04
±6.
02
330
Universitas Sumatera Utara
Lampiran 9. Lanjutan
EE
KS
400
mg/
kg
bb
20,
00
18,
28
18,
58
18,
60
20,
20
26,
36
19,
67
20,
65
24,
13
18,
89
28,
48
22,
16
24,
78
25,
87
23,
45
33,
03
25,
21
25,
37
32,
26
28,
01
33,
94
29,
36
28,
91
37,
21
30,
62
40,
91
35,
46
31,
27
37,
50
35,
18
42,
42
36,
01
34,
81
43,
31
37,
13
40,
91
40,
17
39,
53
41,
57
40,
72
37,
27
35,
46
43,
07
39,
53
38,
11
33,
64
29,
36
38,
64
33,
14
31,
27
28,
18
26,
04
27,
14
30,
52
28,
66
23,
94
24,
10
23,
60
26,
16
21,
50
19,
13
21,
94
24,
95
28,
78
32,
01
36,
06
38,
74
40,
58
38,
69
33,
21
28,
11
23,
86
±0.
90
13,
07
15,
85
15,
96
13,
21
14,
29
±3.
18
16,
48
19,
51
21,
08
17,
36
20,
00
±2.
42
18,
75
21,
95
25,
60
23,
06
24,
68
±3.
71
26,
42
24,
70
29,
52
28,
76
28,
05
±3.
51
26,
99
29,
27
32,
53
31,
35
31,
45
±3.
52
29,
55
31,
10
34,
04
34,
72
32,
47
±3.
87
30,
11
32,
32
33,
43
36,
27
34,
29
±0.
77
27,
84
30,
18
31,
93
27,
46
28,
57
±2.
86
23,
58
25,
91
30,
42
28,
24
26,
75
±3.
47
21,
88
25,
61
29,
22
25,
13
22,
86
±1.
69
17,
33
21,
65
26,
81
22,
02
20,
26
±1.
66
14,
20
16,
16
21,
99
19,
95
18,
18
Rat
arata
14,
48
18,
89
22,
81
27,
49
30,
31
32,
38
33,
28
29,
20
26,
98
24,
94
21,
61
18,
10
SD
±1.
39
±1.
91
±2.
67
±1.
93
±2.
20
±2.
11
±2.
29
±1.
85
±2.
56
±2.
85
±3.
44
±3.
06
30
60
Persen Inhibisi Radang pada Menit ke-n (%)
90
120 150 180 210 240 270 300
330
360
63,7
3
64,6
9
52,2
2
42,3
0
38,0
1
37,1
5
42,1
3
51,3
9
58,0
0
62,8
2
59,2
7
75,3
3
8,22
26,6
8
22,5
0
20,4
3
18,4
3
14,3
5
11,4
8
9,50
5,03
9,29
10,9
3
15,6
0
37,0
9
43,9
8
39,7
6
34,4
4
30,7
4
26,7
2
24,8
9
24,9
9
29,7
2
39,2
6
45,7
1
52,1
0
52,3
8
48,9
6
44,9
3
37,3
8
34,4
2
34,2
0
35,4
8
46,0
2
50,9
9
54,3
9
58,2
7
63,6
6
1
2
3
4
5
Rat
arata
SD
EE
KS
600
mg/
kg
bb
1
2
3
4
5
Kelomp
ok
Natrium
diklofena
k
2,50
mg/kg bb
EEKS
200
mg/kg bb
EEKS
400
mg/kg bb
EEKS
600
mg/kg bb
Universitas Sumatera Utara
Lampiran 10. Hasil uji statistik persen radang dengan SPSS
Menit ke-30
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Std.
Deviation Error
5
30.4100
4.55328
2.03629 24.7564 36.0636 26.04
37.22
Na
diklofenak
2,50 5
mg/kg bb
EEKS 200 mg/kg bb
5
11.0260
2.77550
1.24124 7.5798
12.96
27.9080
1.78819
.79970
25.6877 30.1283 26.13
30.58
EEKS 400 mg/kg bb
5
19.1320
.89550
.40048
18.0201 20.2439 18.28
20.20
EEKS 600 mg/kg bb
5
14.4760
1.38783
.62066
12.7528 16.1992 13.07
15.96
Total
25
20.5904
8.01628
1.60326 17.2814 23.8994 6.44
Sum of Squares
df
Mean Square
F
Sig.
1404.812
137.446
1542.257
4
20
24
351.203
6.872
51.104
.000
Na CMC 0,5%
Lower
Bound
Upper
Bound
Minimum Maximum
14.4722 6.44
37.22
ANOVA
% radang kaki tikus
Between Groups
Within Groups
Total
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
Na diklofenak 2,50 mg/kg bb
EEKS 600 mg/kg bb
EEKS 400 mg/kg bb
EEKS 200 mg/kg bb
Na CMC 0,5%
Sig.
N
1
5
5
5
5
5
2
3
11.0260
14.4760
19.1320
.051
1.000
27.9080
30.4100
.147
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-60
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Std.
Deviation
Mean
Lower
Bound
Std. Error
Upper
Bound Minimum Maximum
Na CMC 0,5%
5 39.1660
3.13675
1.40280
35.2712 43.0608
35.24
43.53
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 13.8320
2.61538
1.16963
10.5846 17.0794
9.51
16.01
5 28.7200
2.77240
1.23985
25.2776 32.1624
26.13
32.78
EEKS 400 mg/kg bb
5 21.9400
3.18009
1.42218
17.9914 25.8886
18.89
26.36
EEKS 600 mg/kg bb
5 18.8860
1.90798
.85327
16.5169 21.2551
16.48
21.08
Total
25 24.5088
9.30134
1.86027
20.6694 28.3482
9.51
43.53
ANOVA
% radang kaki tikus
Sum of Squares
Between Groups
Within Groups
Total
df
1923.882
152.476
2076.358
Mean Square
4
20
24
F
480.970
7.624
Sig.
63.088
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
Na diklofenak 2,50 mg/kg bb
EEKS 600 mg/kg bb
EEKS 400 mg/kg bb
EEKS 200 mg/kg bb
Na CMC 0,5%
Sig.
N
1
5
5
5
5
5
2
3
4
13.8320
18.8860
21.9400
28.7200
1.000
.096
1.000
39.1660
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-90
Descriptives
% radang kaki tikus
95% Confidence Interval
for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5 41.4220
4.35232 1.94642
36.0179
46.8261
37.47
48.26
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 19.7880
1.60582
.71815
17.7941
21.7819
17.18
21.17
5 32.1000
3.39035 1.51621
27.8903
36.3097
28.53
37.10
EEKS 400 mg/kg bb
5 24.9480
2.41714 1.08098
21.9467
27.9493
22.16
28.48
EEKS 600 mg/kg bb
5 22.8080
2.67239 1.19513
19.4898
26.1262
18.75
25.60
Total
25 28.2132
8.38204 1.67641
24.7533
31.6731
17.18
48.26
Sum of Squares
df
ANOVA
% radang kaki tikus
Mean Square
Between Groups
Within Groups
1502.207
184.000
4
20
Total
1686.207
24
F
375.552
9.200
Sig.
40.821
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
Na diklofenak 2,50 mg/kg bb
EEKS 600 mg/kg bb
EEKS 400 mg/kg bb
EEKS 200 mg/kg bb
Na CMC 0,5%
Sig.
5
5
5
5
5
19.7880
22.8080
2
3
4
22.8080
24.9480
32.1000
.131
.278
1.000
41.4220
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-120
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5
43.8960
5.21998 2.33445
37.4145
50.3775
38.71
51.10
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5
25.3260
4.01525 1.79567
20.3404
30.3116
20.65
30.37
5
34.9300
3.12408 1.39713
31.0509
38.8091
30.53
39.03
EEKS 400 mg/kg bb
5
28.7760
3.71277 1.66040
24.1660
33.3860
25.21
33.03
EEKS 600 mg/kg bb
5
27.4900
1.93497
.86534
25.0874
29.8926
24.70
29.52
Total
25
32.0836
7.66307 1.53261
28.9204
35.2468
20.65
51.10
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
1126.707
282.636
4
20
Total
1409.343
24
F
281.677
14.132
19.932
Sig.
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
Na diklofenak 2,50 mg/kg bb
5
25.3260
EEKS 600 mg/kg bb
5
27.4900
EEKS 400 mg/kg bb
5
28.7760
EEKS 200 mg/kg bb
5
Na CMC 0,5%
5
Sig.
3
34.9300
43.8960
.184
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-150
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5 46.2180
6.43202 2.87649
38.2316
54.2044
39.21
54.57
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 28.6460
3.41405 1.52681
24.4069
32.8851
25.08
32.23
5 37.6980
4.37820 1.95799
32.2617
43.1343
32.09
42.58
EEKS 400 mg/kg bb
5 32.0080
3.51175 1.57050
27.6476
36.3684
28.91
37.21
EEKS 600 mg/kg bb
5 30.3180
2.20303
.98522
27.5826
33.0534
26.99
32.53
Total
25 34.9776
7.58111 1.51622
31.8483
38.1069
25.08
54.57
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
1021.834
357.524
4
20
Total
1379.357
24
F
255.458
17.876
14.290
Sig.
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
Na diklofenak 2,50 mg/kg bb
5
28.6460
EEKS 600 mg/kg bb
5
30.3180
EEKS 400 mg/kg bb
5
32.0080
EEKS 200 mg/kg bb
5
Na CMC 0,5%
5
Sig.
3
37.6980
46.2180
.248
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-180
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5 49.2080
6.74378 3.01591
40.8345
57.5815
40.94
58.68
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 30.9340
6.08289 2.72035
23.3811
38.4869
25.38
39.88
5 42.1460
2.81768 1.26011
38.6474
45.6446
39.06
45.73
EEKS 400 mg/kg bb
5 36.0640
3.52401 1.57598
31.6884
40.4396
31.27
40.91
EEKS 600 mg/kg bb
5 32.3760
2.11462
.94569
29.7503
35.0017
29.55
34.72
Total
25 38.1456
8.08620 1.61724
34.8078
41.4834
25.38
58.68
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
1140.042
429.239
4
20
Total
1569.281
24
F
285.011
21.462
13.280
Sig.
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
Na diklofenak 2,50 mg/kg bb
5
30.9340
EEKS 600 mg/kg bb
5
32.3760
EEKS 400 mg/kg bb
5
36.0640
EEKS 200 mg/kg bb
5
Na CMC 0,5%
5
Sig.
3
36.0640
42.1460
49.2080
.112
.051
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-210
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5 51.5820
7.92161 3.54265
41.7460
61.4180
43.67
61.83
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 29.8480
7.11108 3.18017
21.0184
38.6776
24.43
40.49
5 45.6600
1.43040
.63970
43.8839
47.4361
43.44
47.38
EEKS 400 mg/kg bb
5 38.7360
3.87031 1.73085
33.9304
43.5416
34.81
43.31
EEKS 600 mg/kg bb
5 33.2840
2.28873 1.02355
30.4422
36.1258
30.11
36.27
Total
25 39.8220
9.40137 1.88027
35.9413
43.7027
24.43
61.83
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
1578.927
542.332
4
20
Total
2121.259
24
F
394.732
27.117
Sig.
14.557
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
3
Na diklofenak 2,50 mg/kg bb
5
29.8480
EEKS 600 mg/kg bb
5
33.2840
EEKS 400 mg/kg bb
5
EEKS 200 mg/kg bb
5
45.6600
Na CMC 0,5%
5
51.5820
Sig.
33.2840
38.7360
.309
.113
.087
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-240
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound Minimum Maximum
Na CMC 0,5%
5 54.1000
7.94037 3.55104
44.2407 63.9593
45.91
65.62
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 26.3040
6.17229 2.76033
18.6401 33.9679
20.52
36.81
5 48.9640
3.06799 1.37205
45.1546 52.7734
45.94
53.55
EEKS 400 mg/kg bb
5 40.5800
.77123
.34491
39.6224 41.5376
39.53
41.57
EEKS 600 mg/kg bb
5 29.1960
1.84982
.82726
26.8991 31.4929
27.46
31.93
25 39.8288 11.86136 2.37227
34.9327 44.7249
20.52
65.62
Total
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
2918.300
458.304
4
20
Total
3376.604
24
F
729.575
22.915
Sig.
31.838
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
3
Na diklofenak 2,50 mg/kg bb
5
26.3040
EEKS 600 mg/kg bb
5
29.1960
EEKS 400 mg/kg bb
5
EEKS 200 mg/kg bb
5
48.9640
Na CMC 0,5%
5
54.1000
Sig.
40.5800
.351
1.000
.105
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-270
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5 55.0460
8.44181 3.77529
44.5641
65.5279
48.39
68.77
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 23.1160
6.02513 2.69452
15.6348
30.5972
18.89
33.74
5 52.2820
2.39207 1.06977
49.3118
55.2522
48.76
54.52
EEKS 400 mg/kg bb
5 38.6880
2.85813 1.27820
35.1392
42.2368
35.46
43.07
EEKS 600 mg/kg bb
5 26.9800
2.55827 1.14409
23.8035
30.1565
23.58
30.42
25 39.2224 13.94124 2.78825
33.4677
44.9771
18.89
68.77
Total
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
4152.588
512.008
4
20
Total
4664.596
24
F
1038.147
25.600
Sig.
40.552
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
3
Na diklofenak 2,50 mg/kg bb
5
23.1160
EEKS 600 mg/kg bb
5
26.9800
EEKS 400 mg/kg bb
5
EEKS 200 mg/kg bb
5
52.2820
Na CMC 0,5%
5
55.0460
Sig.
38.6880
.241
1.000
.398
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-300
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound
Minimum Maximum
Na CMC 0,5%
5
54.6760
4.26730 1.90840
49.3774
59.9746
51.52
61.83
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5
20.3340
6.02742 2.69555
12.8500
27.8180
15.93
30.67
5
49.6040
4.17740 1.86819
44.4171
54.7909
44.27
54.19
EEKS 400 mg/kg bb
5
33.2100
3.47350 1.55340
28.8971
37.5229
29.36
38.64
EEKS 600 mg/kg bb
5
24.9400
2.85015 1.27463
21.4011
28.4789
21.88
29.22
Total
25
36.5528 14.30041 2.86008
30.6499
42.4557
15.93
61.83
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
4539.326
368.716
4
20
Total
4908.041
24
F
1134.831
18.436
Sig.
61.556
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
3
Na diklofenak 2,50 mg/kg bb
5
20.3340
EEKS 600 mg/kg bb
5
24.9400
EEKS 400 mg/kg bb
5
EEKS 200 mg/kg bb
5
49.6040
Na CMC 0,5%
5
54.6760
Sig.
33.2100
.105
1.000
.077
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-330
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
Lower
Bound
Upper
Bound
5.28709 2.36446
45.2172
58.3468
45.16
56.78
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 21.0900 21.53284 9.62978
-5.6466
47.8266
9.63
59.51
5 46.1200
4.34837 1.94465
40.7208
51.5192
42.37
53.23
EEKS 400 mg/kg bb
5 28.1280
1.68550
.75378
26.0352
30.2208
26.04
30.52
EEKS 600 mg/kg bb
5 21.6140
3.44059 1.53868
17.3419
25.8861
17.33
26.81
25 33.7468 16.05653 3.21131
27.1190
40.3746
9.63
59.51
N
Na CMC 0,5%
Total
Mean
5 51.7820
Std.
Deviation
Std.
Error
Minimum Maximum
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
4086.674
2100.814
4
20
Total
6187.488
24
F
1021.669
105.041
Sig.
9.726
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
Na diklofenak 2,50 mg/kg bb
5
21.0900
EEKS 600 mg/kg bb
5
21.6140
EEKS 400 mg/kg bb
5
28.1280
EEKS 200 mg/kg bb
5
46.1200
Na CMC 0,5%
5
51.7820
Sig.
.317
.393
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 10. Lanjutan
Menit ke-360
Descriptives
% radang kaki tikus
95% Confidence
Interval for Mean
N
Std.
Deviation
Mean
Std.
Error
Lower
Bound
Upper
Bound
Minimum
Maximum
Na CMC 0,5%
5 49.8080
2.00967
.89875
47.3127
52.3033
47.39
52.37
Na diklofenak 2,50
mg/kg bb
EEKS 200 mg/kg bb
5 12.2940
7.42214 3.31928
3.0782
21.5098
6.19
25.15
5 42.0360
6.02099 2.69267
34.5600
49.5120
35.51
51.61
EEKS 400 mg/kg bb
5 23.8600
1.65765
.74132
21.8018
25.9182
21.50
26.16
EEKS 600 mg/kg bb
5 18.0960
3.06327 1.36994
14.2924
21.8996
14.20
21.99
25 29.2188 15.22803 3.04561
22.9330
35.5046
6.19
52.37
Total
ANOVA
% radang kaki tikus
Sum of Squares
df
Mean Square
Between Groups
Within Groups
5135.390
430.043
4
20
Total
5565.433
24
F
1283.848
21.502
Sig.
59.708
.000
% radang kaki tikus
Duncana
Subset for alpha = 0.05
dosis perlakuan
N
1
2
Na diklofenak 2,50 mg/kg bb
5
12.2940
EEKS 600 mg/kg bb
5
18.0960
EEKS 400 mg/kg bb
5
EEKS 200 mg/kg bb
5
Na CMC 0,5%
5
Sig.
3
4
18.0960
23.8600
42.0360
49.8080
.062
.063
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 11. Perhitungan AUC
V1+V0
V2+V1
V12+V11
AUC = V0 �t1-t0� + V1 �t2-t1� + … … … . + V11 (t12 – t11)
=
3.40+3.01
3.01
3.87+3.63
(30 − 0) +
(120 − 90) +
3.63
4.03 +4.03
4.03
3.41+3.40
(60 − 30) +
3.40
3.98 + 3.87
(210 − 180) +
3.41
(150 − 120) +
3.87
3.77 + 4.03
4.03
3.63+3.41
(90 − 60) +
4.03 + 3.98
(240 − 210) +
(180 − 150) +
3.98
3.62 + 3.77
3.77
3.52 + 3.62
4.43 + 3.52
240) + 3.62 (300 − 270) + 3.52 (330 − 300) +
3.35 + 4.43
(270 −
(360 − 330)
Rata-rata = 68,89 + 60,09 + 61,94 + 61,98 + 60,85 + 60,38 + 60,00 + 58,06 +
58,80 + 59,17 + 67,76 +52,69 = 725,61
AUC
4.43
Na.diklofenak
2,50 mg/kg
bb
CMC Na
0,5%
EEKS 200
mg/kg bb
EEKS 400
mg/kg bb
EEKS 600
mg/kg bb
Tikus 1
Tikus 2
Tikus 3
Tikus 4
Tikus 5
725.61
663.11
723.13
728.38
722.21
733.45
732.95
731.69
734.45
733.41
730.32
731.85
737.99
725.48
736.08
729.15
732.46
730.03
729.62
729.54
719.50
721.97
726.42
725.88
723.44
Rata-rata
712.46
733.19
732.34
730.16
723.44
Kelompok
Universitas Sumatera Utara
Lampiran 11. Lanjutan
Descriptives
AUC
95% Confidence Interval for
Mean
N
Na.diklofenak
Mean
Std. Deviation Std. Error Lower Bound Upper Bound Minimum Maximum
5 712.4880
CMC Na 0,5 %
27.70694 12.39092
678.0853
746.8907
663.11
728.38
5 733.2440
1.02882
.46010
731.9665
734.5215
731.69
734.45
EEKS 200 mg/kg bb
5 732.3440
4.93179
2.20556
726.2204
738.4676
725.48
737.99
EEKS 400 mg/kg bb
5 730.1600
1.32316
.59173
728.5171
731.8029
729.15
732.46
EEKS 600 mg.kg bb
5 723.4420
2.85125
1.27512
719.9017
726.9823
719.50
726.42
Total
25 726.3356
14.00097
2.80019
720.5563
732.1149
663.11
737.99
Sum of Squares
df
ANOVA
AUC
Mean Square
Between Groups
1492.909
4
373.227
Within Groups
3211.744
20
160.587
Total
4704.654
24
F
2.324
Sig.
.092
AUC
Duncana
Subset for alpha = 0.05
kelompok perlakuan
N
1
2
Na.diklofenak
5
712.4880
EEKS 600 mg.kg bb
5
723.4420
EEKS 400 mg/kg bb
5
730.1600
EEKS 200 mg/kg bb
5
732.3440
CMC Na 0,5
5
733.2440
Sig.
.187
723.4420
.276
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara