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E. EARLIER STUDIES ON PLANT STEROLS INTAKE
Dietary intake of plant sterols has been sparsely studied, probably depending on the lack of analysed food items in general nutritional databases Klingberg 2012. In the European community,
the  daily  plant  sterols  intake  was  usually  in  the  range  of  200  Morton  et  al.  1995  -  350  mgday Schothorst    Jekel  1999.  Other  research  reported  by  Klingberg  2012  revealed  that  the  plant
sterols intake in Britain was 300 mgday in men and 293 mgday in women while in Sweden was 252 mgday  in  men  and  212  mgday  in  women.  Another  study  conducted  in  China  showed  the  plant
sterols intake level of 322.1 mgday Han et al. 2007. Estimated energy-adjusted plant sterol intake ranges  from  30  to  35  mgMJ,  with  women  having  higher  intakes  than  men  Valsta  et  al.  2004.
Studies of vegetarian and vegan diets showed that compared to a mixed diet, the plant sterol content of these diets is usually higher Piironen et al. 2000.
Study in plant sterols dietary intake is one of observational study  which mainly carried out by either cross-sectional or cohort design.  Most of plant sterols dietary intake studies  were done by
cross-sectional design, which involves observation of all of a population at one specific point in time Andersson et al. 2004, Valsta et al. 2004, Wang et al. 2012. Estimating dietary plant sterols intake
was done similarly with other intake, by using food consumption data of respondents, to which plant sterols values will be assigned using database of plant sterols level in food. The individual or national
food consumption data could be obtained from the databases available Hirai et al. 1986, Morton et al. 1995, Valsta et al. 2004, Han et al. 2007 or by performing food consumption survey using Food
Frequency Questionaire Andersson et al. 2004, Jiménez-Escrig et al. 2006, Sanclemente et al. 2009, Wang  et  al.  2012.  Besides  food  frequency  questionnaire,  other  individual  survey  method  such  as
duplicate 24-hour Recall method could also be performed Schothorst  Jekel 1999. The summary of design, method, and results of several previous studies on plant sterols intake is shown in Table 4.
F. FOOD CONSUMPTION AND INTAKE
The Indonesian Act No. 7 of 1996 explains the food as anything originated from biological sources  and  water,  whether  prepared  or  raw,  which  is  intended  as  food  or  drink  for  human
consumption,  including  food  additives,  food  raw  materials,  and  other  addition  used  in  the manufacturing, processing, and or preparation of food or drink. Food is one of the human basic needs
as  a  source  of  energy  and  nutrients  Bender  2002.  The  essential  nutrients  are  carbohydrates,  fat, protein, vitamins, and minerals Almatsier 2003. Water, although not a nutrient is also essential for
the body. On the other hand, there are also non-nutritional substances that can also be beneficial for the body. For examples are fiber, antioxidants, and food sterols.
Energy and nutrient needs depend on factors such as age, gender, weight, weight, physical activity,  socioeconomic,  and  geographic  conditions  Harper  et  al.  1986;  DeBruyne,  Pinna,
Whitney  2008.  Since  energy  and  nutrient  intake  are  largely  determined  by  the  type  of  food consumed, the type of food that should be consumed will also depend on these factors. Worthington
and  Roberts  2000  divided  the  factor  influencing  food  consumption  into  two  groups;  internal  and external factors. Internal factors are factors such as the needs of the individual physiology and habits.
Meanwhile,  external  factors  are  beyond  individual  factors,  such  as  food  availability  and socioeconomic conditions that affect the food accessibility to someone.
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Table 4. Summary of design, method, and results of several previous studies on plant sterols intake No
Objective Design and Method
Results Publication
Design Dietary survey
Data of PS value in foods 1
Studying  PS  intakes  in  relation to colorectal cancer risk
Cohort Dietary consumption
collected from Food Frequency Questionnaire
FFQ -
Compiled from external source
- Derived from calculation
of recipe -
Laboratory Analysis -
PS intakes for men and women respectively were 310 and 303 mgd.
- A  high  dietary  intake  of  plant  sterols  was  not
associated  with  a  lower  risk  of  colon  and  rectal cancers
Normen  et al. 2001
2 Providing an up-to-date data of
PS intake in Finland Cross
sectional Data obtained from
national databases, which collected by 48  hours
dietary recall and 3 days food record
- Compiled from external
source -
Derived from calculation of recipe
- Laboratory Analysis
PS intake in men was slightly higher than women, i.e. 305 and 237 mgd, respectively.
Valsta et al. 2004
3 Estimating PS intake and
relationship with serum cholesterol concentration in
EPIC Norfolk population Cross-
sectional Dietary consumption
collected from Food Frequency Questionnaire
FFQ -
Compiled from external source
- Derived from calculation
of recipe -
PS intakes for men and women respectively were 310 and 303 mgd.
- Significant negative trend between total blood and
LDL cholesterol and PS intake Andersson
et al. 2004
4 Evaluating
PS intake
and relationship
with serum
cholesterol concentration
in northern Sweden
Cross- sectional
Dietary consumption collected from Food
Frequency Questionnaire FFQ
- Compiled from external
source -
Derived from calculation of recipe
- Laboratory Analysis
- Men had a mean plant sterol intake of 252 mgd,
which  was  significantly  higher  than  women,  212 mgd P 0.001.
- Higher  plant  sterol  density  was  associated  with
lower  serum  total  cholesterol  in  both  men  and women  and  with  lower  LDL  cholesterol  in
women Klingberg
et al. 2008.
5 Evaluating
the associations
between  natural  dietary  intake of  PS  and  carotid  intima
–media thickness  IMT  and  serum
lipids in Chinese adult. Cross-
sectional Dietary
consumption collected
from Food
Frequency  Questionnaire FFQ
- Obtained  from  database
available -
Men had higher PS intake 330 mgd than women 311 mgd p= 0.007.
- Multivariate  analysis  showed  a  dose-dependent
inverse  association  of  total  PS  intake  with  serum TC,  LDLc,  non-HDLc  in  highest  quartile  of
intake 447 mgd. Wang  et  al.
2012
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G. FOOD CONSUMPTION SURVEY