ada suhu 40
o
C dan pH 5.0. Spesifitas xilanase mobil terhadap xilan birchwood menghasilkan DP antara 37–7, dan pada xilan
apat digunakan berulang, namu
V. SIMPULAN DAN SARAN
5.1. Simpulan
Amobilisasi xilanase dari Streptomyces sp. isolat 45I-3 dilakukan
menggunakan larutan Eudragit S100 konsentrasi 1 bv. Rasio volume Xilanase Ekstrak Kasar dengan Larutan Eudragit S100 1 bv yang digunakan
adalah 5:1. Amobilisasi menyebabkan penurunan aktivitas xilanase menjadi 23.97 ,
serta perubahan pH dan suhu optimum. Xilanase amobil memiliki aktivitas optimum pada pH 6.0 dan suhu 40
o
C. Xilanase amobil stabil selama 1 jam pada suhu 30–40
o
C, pH 6.0, serta stabil pada substrat xilan birchwood dan xilan tongkol jagung selama 6 jam pada pH 6.0 dan suhu 40
o
C. Xilanase amobil lebih stabil pada substrat xilan birchwood dibandingkan xilan tongkol jagung. Xilanase
amobil juga stabil selama 5 jam p a
tongkol jagung antara 122–26. Xilanase amobil d n terjadi penurunan aktivitas menjadi 52.38 setelah 3 kali penggunaan.
Xilanase amobil memiliki potensi untuk diaplikasikan pada pembuatan beragam xilooligosakarida, terutama karena keleluasaan dalam pengendalian
prosesnya.
5.2. Saran
Amobilisasi xilanase dari Streptomyces sp. 45I-3 memiliki efektivitas
amobilisasi yang rendah sehingga perlu dikombinasikan dengan metode pengikatan kovalen sehingga dapat meningkatkan efektivitas amobilisasi dan
meningkatkan stabilitasnya.
DAFTAR PUSTAKA
Alonso JL, Dominguez H, Garrote G, Parajo C, Vasquez MJ. 2003. Xylooligosaccharides: Properties and Production Technology.
EJEAFChe 21:230–232. [Anonim] 2004. Product Range Eu
TM
m 21 Februari 2
Abdel-Naby β-
Abde xtrin
34:
Ai Z, yces
Alexander M. 1961. Introduction to Soil Microbiology. Jepang: John Wiley and Sons Inc.
Anggraini F. 2003. Kajian Ekstraksi dan Hidrolisis Xilan dari Tongkol Jagung Zea mays L.
[skripsi]. Bogor: Fakultas Teknologi Pertanian, Institut Pertanian Bogor. Beg QK, Kapoor M, Mahajan L, Hoondal GS. 2001. Microbial Xylanases and Their Industrial
Applications. [ulasan]. Appl Microbiol Biotecnol 56:326–338.
Bernier R, Desrochers M, Jurasel L, Paice MG. 1983. Isolation and Characterization of A Xylanase from
Bacillus subtilis. Appl and Environ Microbiol 462:511–514. Bradford MM. 1976. A Rapid and Sensitive Method for The Quantitation of Microgram
Quantities of Protein Utilizing The Principle of Protein-dye Binding. Anal Biochem
72:91–96. Cano A, Minguillón C, Palet C. 2006. Immobilization of Endo-1,4-
β-Xylanase on Polysulfone Acrylate Membranes: Synthesis and Characterization.
J of Membrane Sci 280:383– 388.
Chen C, Chen JL, Lin TY. 1997. Purification and Characterization of Xylanase from Trichoderma longibrachiatum for Xylooligosaccharides Production. Enzyme Microb
Technol 21:91–96.
Collins T, Gerday C, Feller G. 2005. Xylanases, Xylanase Families and Extremophilic Xylanases.
FEMS Microbiol Rev 29:3–23. J
dragit [terhubung berkala] www.degussa4pharma.co 004.
MA. 1993. Immobilization of Aspergillus niger NRC-107 Xylanase and
Xylosidase and Properties of The Immobilized Enzyme. Appl Biochem and Biotechnol
38:69–81. l-Naby MA. 1999. Immobilization of
Paenibacillus macerans NRRL B-3186 Cyclode Glucosytransferase and Properties of The Immobilized Enzyme.
Process Biochem 399–405.
Jiang Z, Li L, Deng W, Kusakabe I, Li H. 2005. Immobilization of Streptom
olivaceoviridis E-86 Xylanase on Eudragit
TM
S100 for Xylo-Oligosaccharide Production. Process Biochem 40:2707–2714.
Dubois M, Guilles KA, Ham 56. Colorimetric Method for
m 28:350–356. Dumitriu S, Chornet E. 1997. Immobilization of Xylanase in Chitosan-Xanthan Hydrogels.
Biotec Edwa
rom : 11-
Eijk J H Va bility
Enga ized
Feng ono,
Gaur ium
Gawande P for
Produ Gawande PV, Kamat MY. 1998b. Preparation, Characterization and Application of
Aspergillus sp. Xylanase Immobilized on Eudragit
TM
S100. J of Biotechnol 66:165–175.
Gawande PV, Kamat MY. 1999. Purification of Aspergillus sp. Xylanase by Precipitation with
an Anionic
TM
Geori of
Enzym Microb Technol 26:178-
Goel MK. 1994. Immobilized Enzyme [terhubung berkala] http:www.rpi.edudeptchem- engBiotech-EnvironIMMOBgoel2nd.htm 2 Februari 2006.
Gouda MK, Abdel-Naby M. 2002. Catalytic Properties of The Immobilized Aspergillus tamarii
Xylanase. Microbiol Res 157:275–281. Haapala P, Linko S, Parkinnen E, Suominen P. 1994. Production of Endo-1,4-Glucanase and
Xylanase by Trichoderma reesei Immobilized on Polyurethane Foam. Biotechnol Tech
86:401–406. Harper HA, Rodwel VW, Mayer PA. 1984.
Review of Physiological Chemistry. California: Lange Medical Publication.
ilton JK, Rebers PA, Smith F. 19 ed Substances.
Anal Che Determination of Sugars and Relat
hnol Prog 13:539–545. rd VA, Pillay VL, Swart P, Singh S. 2002. Immobilization of Xylanase f
Thermomyces lanuginosus SSBP using Eudragit
TM
S100. South African J of Sci 98
12 n, Quax WJ, Sanders JPM. 1991. Mutant Enzyme Having Reduced Sta
under Industrial Application Conditions. European patent appl EP 0.409.299.
sser JM, Horvath C. 1976. Applied Biochemistry and Bioengineering Immobil
Enzyme Principle. New York:Academic Press. Hal 127. el D, Wegener. 1995.
Wood: Chemistry, Ultrastructure, Reactions. S. Hardj penerjemah. Yogyakarta: UGM Press.
R, Lata, Khare SK. 2005. Immobilization of Xylan-degrading Enzymes from Scytalid
thermophilum on Eudragit
TM
L100. World J of Microbiol and Biotechnol 21:1123–1128.
V, Kamat MY. 1998a. Immobilization of Aspergillus sp. on Nylon Bolting Cloth
ction of Xylanase. J of Fermentation and Bioeng 862:243–246.
Polymer Eudragit S100. Process Biochem 34:577–580.
s J, Giannotta F, De Buylb E, Grainer B, Fre’re JM. 2000. Purification and Properties Three Endo-
β-1,4-Xylanases Produced by Streptomyces sp. Strain S38 Which Differ in Their Ability to Enhance The Bleaching of Kraft Pulp.
186
Hasim. 2003. Menanam Rumput-M as 3 November 2003:11.
Science. University of The Sunshine Coast.
Howa n Resenburg EL, Howard S, 2003. Lignocellulose Biotechnology:
Issues of Bioconversion and Enzyme Production. African J Biotechnol 212:602–619.
Huan tabolic Functions.
Ann Nutr Metab 47:319–666.
Neurospora
n idis
E-86. Biosci Biotechnol Biochem 64:447–451.
ns Streptomyces
galbus NR and Their Use in Biobleaching of Softwood Kraft Pulp. Antonie van
im SW. 2005. Development of Enzyme Immobilization Technique [terhubung berkala]
oshland DE. 1958. Application of a Theory of Enzyme Specificity to Protein Synthesis. Proc
Kraje szko M, Zaborska W. 1990. Urease Immobilized on Chitosan Membrane:
Preparation and Properties. J Chem Technol Biotechnol 48:337–350.
Kulka Microbiol Rev 23:411–456.
NS, Cheah HY, Wong NKI, Ho CC. 2002. Actinomycetes Isolated from Soil 2002.
emanen Antibiotik. Komp
Hayakawa M. 2003. Selective Isolation of Rare Actinomycetes Genera using Pretreatment Technique. Di dalam:Larissa Terekhova, Takao Okazaki, Ipek Kurtboke, editor.
Selective Isolation of Rare Actinomycetes. Queensland: Faculty of
rd RL, Abotsi E, Va
g YJ. 2003. The Effects of Xylooligosaccharides on Blood Glucose Tolerance and its Related Me
Hyndmann DJ, Burrell R, Lever G, Flynn TG. 1992. Protein Immobilization to Alumina Supports via Organophosphate Linkers.
Biotechnol Bioeng 40:1328–1336. Irawadi TT. 1991. Produksi Enzim Ekstraseluler Selulosa dan Xilanase dari
sitophila pada Substrat Limbah Padat Kelapa Sawit. [Disertasi]. Bogor: Program Pasca Sarjana, Institut Pertanian Bogor.
Kaneko S, Kuno A, Muramatsu M, Iwamatsu S, Kusakabe I, Hayashi K. 2000. Purificatio and Characterization of A Family G11
β-Xylanase from Streptomyces olivaceovir
oh AL, Nagieb ZA. 2004. Xylanase and Mannanase Enzymes from Ka
Leeuwenhoek 85:103–114. K
http:www.korea.ac.kr 28 Maret 2005. K
Natl Acad Sci 442:98–104. wska B, Le
rni N, Shendye A, Rao M. 1999. Molecular and Biotechnological Aspect of Xylanases. FEMS
Kusakabe I, Yasui T, Kobayashi T. 1976. Enzymatic Hydrolysis-Extraction of Xylan- Containing Natural Materials 1.
Nippon Nogeikagaku Kaishi 50:199–208. Lo CW, Lai
Samples from The Crocker Range Sabah [terhubung berkala] http:www.arbec.com.my July-September
Magnuson TS dan Crawford DL. 1997. Purification and Characterization of An Alkaline Xylanase from
Streptomyces viridosporus T7A. Enzyme and Microb Technol 21:160- 164.
Martinez-Trujila A, Perez-Avalos O, Ponce-Nayola T. 2003. Enzymatic Properties of A Purified Xylanase from Mutant PN-120 of
Cellulomonas flavigena. Enzyme Microb
Maynard LA, Loosli JK, Hintz HF and Warner RG. 1983. Animal Nutrition. Ed Ke-7. New
Delhi: Hill Publishing Company Limited. Merya
Upaya Menggali Mikrob Penghasil Enzim Komersial [laporan akhir hibah bersaing]. Bogor:
iller GL. 1959. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar.
iyado H. 2003. Prosedur Karakterisasi dan Identifikasi Aktinomisetes. Di Dalam Training
almer T. 1981. Understanding Enzymes. England: Ellis Horwood Ltd.
Perez and Biological
Treatments of Cellulose, Hemicellulose and Lignin. [ulasan]. Int Microbiol 52:53–63.
Pratiw Jagung. [skripsi]. Bogor: Fakultas Teknologi Pertanian, Institut Pertanian Bogor.
Priha Streptomyces sp.
45I-3 asal Kalimantan. [tesis]. Bogor: Program Pascasarjana, Institut Pertanian Bogor. Rao M
ogalski J, Szczodrak J, Dawidowicz A, Ilczuk Z, Leonowicz A. 1985. Immobilization of
bib.html 28 Maret 2005.
oy I, Gupta MN. 2003. Smart Polymeric Materials: Emerging Biochemical Applications. Technol 32:401–406.
ndini A. 2005. Karakterisasi Xilanase Aktinomiset asal Indonesia dalam LPPM, Institut Pertanian Bogor.
M Anal Chem 31:426–428.
M Course on Identification of Bacteria, Bogor, 1-5 April 2003. Bogor: LIPI.
P J, Munoz-Dorado J, de la Rubia T, Martinez J. 2002. Biodegradation
i FMR. 2006. Produksi Xilanase dari Streptomyces sp. Pada Substrat Xilan Tongkol
ndono P. 2007. Purifikasi dan Karakterisasi Xilanase Ekstraseluler
, Mishra C. 1984. Properties and Applications of Penicillium funiculosum Cellulase
Immobilized on A Solluble Polymer. Biotechnol Lett 65:319–322.
R Cellulase and
β-Xylanase Complexes from Aspergillus terrus F-413 on Controlled Porosity Glasses.
Enzyme and Microb Technol 7:395–398. Rosevear A, Kennedy JF, Cabral JMS. 2005a. Enzyme Immobilization [terhubung berkala]
http:alfa.ist.utl.pt~fidelenzymaticgen_ Rosevear A, Kennedy JF, Cabral JMS. 2005b. Choice of Biological Systems [terhubung
berkala] http:alfa.ist.utl.pt~fidelenzymaticappendiximmob.html 28 Maret 2005. R
[ulasan]. Chem and Biol 10:1161–1171.
Roy I, Sharma S, Gupta MN. 2004. Smart Biocatalysts: Design and Applications. [ulasan]. Adv Biochem Eng Biotechnol 86:159-189.
Roy I echnol 61:309–313.
ation of Xylanase Xys 1
from Streptomyces halstedii JM8. Appl and Environ Microbiol 61:2414–2419.
Saha 91.
ardar M, Agarwal R, Kumar A, Gupta MN. 1997. Noncovalent Immobilization of Enzymes
arda us Purification and Immobilization of
Aspergillus niger Xylanase on The Reversibly Soluble Polymer Eudragit
TM
L100.
hule ess Engineering: Basic Concepts. USA: Prentice Hall.
277:413-417.
α-Amylase. J Chem Technol Biotechnol 48:185–200.
ation in Canada, Japan, the European Union and the United States of America. Ottawa: InterSect
ubramaniyan S, Prema P. 2002. Biotechnology of Microbial Xylanases: Enzymology,
uhartono MT. 1989. Enzim dan Bioteknologi. Bogor: PAU Bioteknologi IPB.
Tyagi , Gupta A, Khare SK, Bisaria VS, Gupta MN. 2003. Immobilization of Xylan-Degrading
Enzymes from Melanocarpus albomyces IIS 68 on the Smart Polymer Eudragit
TM
L100. Appl Microbiol Biot
Roy PK, Roy U, Dube DK. 1984. Immobilized Cellulolytic and Hemicellulolytic Enzymes from Macrophomina phaseolina. J Chem Technol Biotechnol 39:165–170.
Ruiz-arribas A, Jose M, Fernandez-abalos, Pilar S, Ana LG, Ramon IS. 1995. Overproduction, Purification and Biochemical Characteriz
BC. 2003. Hemicellulose Bioconversion. J Ind Microbiol Biotechnol 30:279-2
Saha BD. 2001. Purification and Characterization of An Extracellular β-Xylosidase from A
Newly Isolated Fusarium verticillioides. J Indust Microbiol Biotechnol 27:241–245.
S on An Enteric Polymer Eudragit
TM
S100. Enzyme Microb Technol 20:361-367.
r M, Roy I, Gupta MN. 2000. Simultaneo S
Enzyme and Microb Technol 27:672–679. r ML, Kargi F. 2002. Bioproc
S Simpson HD, Haufler UR, Daniel RM. 1991. An Extremely Thermostable Xylanase from The
Thermophilic Eubacterium Thermotoga. Biochem J
Siso MI, Graber M, Condoret JM, Combes D. 1990. Effect of Diffusional Resistance on The Action Pattern of Immobilized
Smith BL, Marcotte M, Harrison G. 1996. A Comparative Analysis of the Regulatory
Framework Affecting Functional Food Development and Commercializ Alliance Inc.
S Molecular Biology and Application.
Crit Rev Biotechnol 221:33–64 . S
T, Gupta MN. 1995. Immobilization of Aspergillus niger Xylanase on Magnetic Latex
Beads. Biotechnol Appl Biochem 21:217–222.
Tyagi R, Roy I, Agarwal R, Gupta MN. 1998. Carbodi-Imide Coupling of Enzymes to The Reversibly Soluble Insoluble Polymer Eudragit
TM
S100. Biotechnol Appl Biochem
28:201–206.
ang SL, Chio SH. 1998. Reversible Immobilization of Chitinase via Coupling to Reversibly
idiastuti F. 2004. Karakterisasi Awal Beberapa Aspek Biomolekuler dari Isolat Xilanolitik
orsfold PJ. 1995. Classification and Chemical Characteristics of Immobilized Enzymes
ang R, Xu S, Wang Z, Yang W. 2005. Aqueous extraction of corncob xylan and production
hengqiang J. 2001. Characterization of A Thermostable Family 10 Endo-Xylanase XynB e.
J Biosci Bioeng 925:423–428.
Zubri Immobilization of Hydrolases onto chitosan microparticles.
Chemija vilnius 144: - Vasquez MJ, Alonso JL, Dominguez H, Parajo JC. 2000. Xylooligosaccharides: Manufacture
and Application. Trends in Food Sci and Technol 11:387–393.
W Soluble Polymer.
Enzyme Microb Technol 22:634–640. W
Termofilik RT-3. [skripsi]. Bogor: Departemen Biologi, Fakultas MIPA, Institut Pertanian Bogor.
W Technical Report IUPAC.
Pure and Appl Chem 674:597–600. Y
of xylooligosaccharides. LWT 38:677-682.
Z from
Thermotoga maritima that Cleaves p-Nitrophenyl-
β
-D-Xylosid
ene A, Budriene S, Gorochovceva N, Romaskevic T, Matulionis E, Dienys G. 2003.
LAMPIRAN
Lampiran 1. Komposisi Bufer Pencuci pada Pembuatan Xilanase Amobil
Jenis Bufer
Ko mpo
sisi
Bufer Aseta
t
t 0.2 M
dala
0.2 M
C
2
H
3
O
2
Na atau
27.2 g C
