52
BAB V SIMPULAN DAN SARAN
A. Simpulan
Berdasarkan hasil dari uraian pembahasan, dapat ditarik kesimpulan sebagai berikut:
1. Konsentrasi logam Cu, Cd dan Pb dalam media cair setengah MS
berpengaruh signifikan terhadap pertambahan panjang akar, namun pada parameter pertambahan jumlah akar logam Cu dan Cd tidak berpengaruh
signifikan. Konsentrasi Cu yang paling optimal dalam menghambat pertumbuhan akar adalah 200 µM, sedangkan konsentrasi Cd yang paling
menghambat pertumbuhan akar yaitu 300 µM dan pada cekaman Pb konsentrasi yang secara maksimal menghambat konsentrasi 100 µM.
2. Terdapat perbedaan tingkat parameter kerusakan anatomi akar, akumulasi
logam dan warna daun pada setiap konsentrasi dan jenis logam dalam media. Ketiga parameter tersebut tidak tergantung satu sama lain, yang berarti
meskipun dicekam oleh logam dan konsentrasi yang sama tiap parameter dapat menunjukan hasil yang berbeda. Pada cekaman logam Cu besarnya
akumulasi logam dalam akar ekuivalen dengan besarnya konsentrasi Cu dalam media. Pada cekaman logam Cd akumulasi logam dalam akar tidak
ekuivalen dengan besarnya konsentrasi Cd dalam media. Pada cekaman logam Pb besarnya akumulasi logam dalam akar ekuivalen dengan besarnya
konsentrasi 3.
Berdasarkan analisis kuantitatif, tembakau mampu mentoleransi cekaman logam Cu pada level ≤ 100 µM, Cd pada konsentrasi 50 µM dan Pb pada
konsentrasi ≤ 5 µM. Berdasarkan analisis kualitatif tembakau mampu mentoleransi cekaman pada konsentrasi logam Cu pada level ≤ 50 µM, Cd
pada konsentrasi 50 µM dan Pb pada konsentra si ≤ 20 µM.
B. Saran
Berdasarkan hasil penelitian yang diperoleh dapat dirumuskan saran sebagai berikut:
1. Untuk penelitian sejenis perlu dilakukan analisis AAS baik pada bagian akar
root maupun bagian batang daun shoot untuk mengetahui secara detail pola translokasi logam.
2. Pada penelitian serupa sebaiknya dilakukan uji lanjut yaitu re-growth analysis
untuk mengetahui responsif tidaknya tembakau terhadap pemulihan pertumbuhan akar pada media nutritif non logam.
3.
Perlu dilakukan penelitian lanjut guna mengetahui respon tembakau Nicotiana tabacum L. secara molekuler dan mikroanatomis terhadap
cekaman logam berat Cu, Cd dan Pb
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LAMPIRAN I
PengambilandanAnalisis Data A.
RekapPengambilan Data
Logam Konsentrasi
µM Pertambahanpanjangakar
ulangan Jumlah
Rata-rata mm
1 2
3 4
5
Cu 6
7 10
4 20
47 9.4
50 8
5 7
9 6
35 7
100 6
5 7
5 6
29 5.8
150 2
5 7
14 2.8
200 3
1 4
4 12
2.4
Cd 17
7 6
9 6
45 9
50 5
3 4
4 6
22 4.4
100 5
4 3
4 4
20 4
200 4
4 4
2 3
17 3.4
300 2
9 6
1 18
3.6
Pb 10
11 15
8 14
58 11.6
5 8
17 9
9 8
51 10.2
20 16
3 3
2 24
4.8 50
1 6
4 2
4 17
3.4 100
3 5
2 2
3 15
3
Logam Konsentrasi
µM Pertambahanjumlahakar
ulangan Jumlah
Rata-rata 1
2 3
4 5
Cu 1
3 9
8 4
25 5
50 3
4 5
4 7
23 4.6
100 8
4 6
4 22
4.4 150
1 4
6 7
18 3.6
200 2
1 2
3 2
10 2
Cd 16
7 5
9 5
42 8.4
50 5
5 3
6 4
23 4.6
100 7
12 4
8 8
39 7.8
200 7
13 10
8 3
41 8.2
300 8
2 3
8 8
29 5.8
Pb 8
7 10
7 5
37 7.4
5 7
3 5
3 6
24 4.8
20 5
2 3
1 4
15 3
50 3
1 6
4 4
18 3.6
100 1
2 3
1 3
10 2
62
B. Hasilanalisisanavasatujalandanuji BNT