PEMERIKSAAN BAKTERI KOLIFORM DALAM AIR M
KARYA TULIS ILMIAH
PEMERIKSAAN BAKTERI KOLIFORM
DALAM AIR MINUM KEMASAN GALON ISI ULANG
MEREK MT, MA, DAN BIO DI KABUPATEN GRESIK
Oleh:
ALFU LAILA, S.Si., Apt
ERFAN YUDAPRAJA, S.Farm., Apt
AKADEMI FARMASI MITRA SEHAT MANDIRI
SIDOARJO
2011
DAFTAR ISI
HALAMAN JUDUL ................................................................................. i
HALAMAN PENGESAHAN ................................................................... ii
HALAMAN PERNYATAAN .................................................................. iii
KATA PENGANTAR ............................................................................... iv
DAFTAR ISI ............................................................................................. vi
DAFTAR TABEL ..................................................................................... ix
DAFTAR GAMBAR ................................................................................. x
DAFTAR LAMPIRAN ............................................................................. xi
INTISARI .................................................................................................. xii
ABSTRAK ................................................................................................. xiii
BAB I PENDAHULUAN ......................................................................... 1
A. ........................................................................................... L
atar Belakang Masalah............................................................... 1
B. ........................................................................................... P
erumusan Masalah ..................................................................... 1
C. ........................................................................................... T
ujuan Penelitian ......................................................................... 2
D. ........................................................................................... M
anfaat Penelitian ........................................................................ 2
BAB II TINJAUAN PUSTAKA ............................................................... 3
A. ........................................................................................... A
ir ................................................................................................. 3
1.1..................................................................................... M
acam-macam Air ................................................................ 4
1.1.1 ............................................................................ A
ir Tanah ................................................................... 4
1.1.2 ............................................................................ A
ir Permukaan .......................................................... 5
B. ........................................................................................... B
akteri .......................................................................................... 5
2.1 ..................................................................................... M
orfologi Bakteri .................................................................. 6
2.1.1 ............................................................................ B
entuk Basil .............................................................. 6
2.1.2 ............................................................................ B
entuk Kokus ............................................................ 6
2.1.3 ............................................................................ B
akteri Benbentuk Melilit ......................................... 7
2.2 ..................................................................................... S
usunan Sel Bakteri .............................................................. 7
C. ........................................................................................... B
akteri Koliform .......................................................................... 8
3.1 ................................................................................. P
enyakit yang ditimbulkan ................................................. 10
3.2 ................................................................................. M
anfaat bakteri Koliform .................................................... 10
D. ........................................................................................... J
umlah Perkiraan Terdekat.......................................................... 10
BAB III METODELOGI PENELITIAN ................................................... 12
A. ........................................................................................... P
opulasi dan Sampel .................................................................... 12
B. ........................................................................................... V
ariabel penelitian........................................................................ 12
C. ........................................................................................... B
ahan dan Alat ............................................................................. 12
3.1 ................................................................................. A
lat Penelitian ..................................................................... 12
3.2 ................................................................................. B
ahan Penelitian ................................................................. 12
D. ........................................................................................... J
alannya Penelitian ...................................................................... 13
4.1 ................................................................................. M
edia ................................................................................... 13
4.2 ................................................................................. P
engambilan Sampel .......................................................... 14
4.3 Penentuan Jumlah Perkiraan terdekat (JPT) Bakteri
Koliform ........................................................................... 14
BAB IV HASIL PENELITIAN DAN PEMBAHASAN
A. ......................................................................................... H
asil
pemeriksaan
jumlah
perkiraan
terdekat
Bakteri
Koliformpada sampel air minum galon isi ulang .................... 16
B. ......................................................................................... P
embahasan ............................................................................... 18
BAB V KESIMPULAN DAN SARAN
A. ......................................................................................... K
esimpulan ................................................................................ 20
B. ......................................................................................... S
aran .......................................................................................... 20
DAFTAR PUSTAKA
21
INTISARI
ALFU LAILA, S.Si., Apt., ERFAN YUDAPRAJA, S.Farm., Apt, 2011,
PEMERIKSAAN BAKTERI KOLIFORM DALAM AIR MINUM KEMASAN
GALON ISI ULANG MEREK MT, MA, DAN BIO DI KABUPATEN GRESIK,
tugas akhir, AKADEMI FARMASI MITRA SEHAT MANDIRI.
Telah diteliti air minum galon isi ulang merek MT, MA, dan BIO yang
diambil dari tiga lokasi di wilayah kabupaten Gresik. Untuk mengetahui jumlah
bakteri koliform dalam 100 ml air galon isi ulang digunakan metode tabung ganda
atau Most Probable Number(MPN). Dari hasil penelitian didapat bahwa ketiga
sample air minum tersebut ditinjau dari segi bakteri koliform-nya tidak memenuhi
persyaratan Menteri Kesehatan RI No 907/MENKES/SK/VII/2002.
Kata kunci: Bakteri Koliform, Air minum, JPT.
ABSTRACT
ALFU LAILA, S.Si., Apt., ERFAN YUDAPRAJA, S.Farm., Apt, 2011,
RESEARCH OF KOLIFORM BACTERIA IN GALLONS DRINKING WATER.
GALLONS BRANDED MT, MA, AND BIO IN GRESIK REGENCY, THESIS,
AKADEMI FARMASI MITRA SEHAT MANDIRI.
Gallonsdrinking waterhas been investigatedbrandedMT, MA,
andBIOtaken fromthree locationsinGresikregency. To findthe number ofcoliform
bacteriain 100mlrefillof gallonsof water, weusedthemethod ofmultipletube or
MostProbableNumber (MPN). From theresultsobtainedthat the threesamplesof
drinking waterdoes not meetthe requirements ofthe Minister of HealthDecree
No.907/MENKES/SK/VII/2002 reviewed in terms ofcoliformbacteria.
Keywords: Koliform bacteria, Drinking Water, JPT.
PEMERIKSAAN BAKTERI KOLIFORM
DALAM AIR MINUM KEMASAN GALON ISI ULANG
MEREK MT, MA, DAN BIO DI KABUPATEN GRESIK
Oleh:
ALFU LAILA, S.Si., Apt
ERFAN YUDAPRAJA, S.Farm., Apt
AKADEMI FARMASI MITRA SEHAT MANDIRI
SIDOARJO
2011
DAFTAR ISI
HALAMAN JUDUL ................................................................................. i
HALAMAN PENGESAHAN ................................................................... ii
HALAMAN PERNYATAAN .................................................................. iii
KATA PENGANTAR ............................................................................... iv
DAFTAR ISI ............................................................................................. vi
DAFTAR TABEL ..................................................................................... ix
DAFTAR GAMBAR ................................................................................. x
DAFTAR LAMPIRAN ............................................................................. xi
INTISARI .................................................................................................. xii
ABSTRAK ................................................................................................. xiii
BAB I PENDAHULUAN ......................................................................... 1
A. ........................................................................................... L
atar Belakang Masalah............................................................... 1
B. ........................................................................................... P
erumusan Masalah ..................................................................... 1
C. ........................................................................................... T
ujuan Penelitian ......................................................................... 2
D. ........................................................................................... M
anfaat Penelitian ........................................................................ 2
BAB II TINJAUAN PUSTAKA ............................................................... 3
A. ........................................................................................... A
ir ................................................................................................. 3
1.1..................................................................................... M
acam-macam Air ................................................................ 4
1.1.1 ............................................................................ A
ir Tanah ................................................................... 4
1.1.2 ............................................................................ A
ir Permukaan .......................................................... 5
B. ........................................................................................... B
akteri .......................................................................................... 5
2.1 ..................................................................................... M
orfologi Bakteri .................................................................. 6
2.1.1 ............................................................................ B
entuk Basil .............................................................. 6
2.1.2 ............................................................................ B
entuk Kokus ............................................................ 6
2.1.3 ............................................................................ B
akteri Benbentuk Melilit ......................................... 7
2.2 ..................................................................................... S
usunan Sel Bakteri .............................................................. 7
C. ........................................................................................... B
akteri Koliform .......................................................................... 8
3.1 ................................................................................. P
enyakit yang ditimbulkan ................................................. 10
3.2 ................................................................................. M
anfaat bakteri Koliform .................................................... 10
D. ........................................................................................... J
umlah Perkiraan Terdekat.......................................................... 10
BAB III METODELOGI PENELITIAN ................................................... 12
A. ........................................................................................... P
opulasi dan Sampel .................................................................... 12
B. ........................................................................................... V
ariabel penelitian........................................................................ 12
C. ........................................................................................... B
ahan dan Alat ............................................................................. 12
3.1 ................................................................................. A
lat Penelitian ..................................................................... 12
3.2 ................................................................................. B
ahan Penelitian ................................................................. 12
D. ........................................................................................... J
alannya Penelitian ...................................................................... 13
4.1 ................................................................................. M
edia ................................................................................... 13
4.2 ................................................................................. P
engambilan Sampel .......................................................... 14
4.3 Penentuan Jumlah Perkiraan terdekat (JPT) Bakteri
Koliform ........................................................................... 14
BAB IV HASIL PENELITIAN DAN PEMBAHASAN
A. ......................................................................................... H
asil
pemeriksaan
jumlah
perkiraan
terdekat
Bakteri
Koliformpada sampel air minum galon isi ulang .................... 16
B. ......................................................................................... P
embahasan ............................................................................... 18
BAB V KESIMPULAN DAN SARAN
A. ......................................................................................... K
esimpulan ................................................................................ 20
B. ......................................................................................... S
aran .......................................................................................... 20
DAFTAR PUSTAKA
21
INTISARI
ALFU LAILA, S.Si., Apt., ERFAN YUDAPRAJA, S.Farm., Apt, 2011,
PEMERIKSAAN BAKTERI KOLIFORM DALAM AIR MINUM KEMASAN
GALON ISI ULANG MEREK MT, MA, DAN BIO DI KABUPATEN GRESIK,
tugas akhir, AKADEMI FARMASI MITRA SEHAT MANDIRI.
Telah diteliti air minum galon isi ulang merek MT, MA, dan BIO yang
diambil dari tiga lokasi di wilayah kabupaten Gresik. Untuk mengetahui jumlah
bakteri koliform dalam 100 ml air galon isi ulang digunakan metode tabung ganda
atau Most Probable Number(MPN). Dari hasil penelitian didapat bahwa ketiga
sample air minum tersebut ditinjau dari segi bakteri koliform-nya tidak memenuhi
persyaratan Menteri Kesehatan RI No 907/MENKES/SK/VII/2002.
Kata kunci: Bakteri Koliform, Air minum, JPT.
ABSTRACT
ALFU LAILA, S.Si., Apt., ERFAN YUDAPRAJA, S.Farm., Apt, 2011,
RESEARCH OF KOLIFORM BACTERIA IN GALLONS DRINKING WATER.
GALLONS BRANDED MT, MA, AND BIO IN GRESIK REGENCY, THESIS,
AKADEMI FARMASI MITRA SEHAT MANDIRI.
Gallonsdrinking waterhas been investigatedbrandedMT, MA,
andBIOtaken fromthree locationsinGresikregency. To findthe number ofcoliform
bacteriain 100mlrefillof gallonsof water, weusedthemethod ofmultipletube or
MostProbableNumber (MPN). From theresultsobtainedthat the threesamplesof
drinking waterdoes not meetthe requirements ofthe Minister of HealthDecree
No.907/MENKES/SK/VII/2002 reviewed in terms ofcoliformbacteria.
Keywords: Koliform bacteria, Drinking Water, JPT.