Penetapan Kadar Asam Benzoat Dalam Sediaan Sirup Multivitamin Secara Spektrofotometri Ultraviolet

29

Lampiran 1
Hasil Kromatografi Lapis Tipis

Universitas Sumatera Utara

30

Spectrophotometric Report
abs

2.000

Data information
File Name: C:\Documents and Settings\
Administrator\My Documents\
OT\Pengawet\Bk. Benzoat 1.pho
Analyst:
System Administrator
Date/Time: 03/01/2012 12:00 PM

1.500

Instruments Information
Instrument Name: Instrument 1
Instrument Type: UV-1800 Series

0.500

1.000

0.000
200.00

220.00 240.00
0

260.00

280.00

300.00

nm

Spetrum
No
1
2
3

P/V

Photometric
Standard Table
No
Sample ID
1
Bk. Benzoat 1
Sample Table

No
Sample ID
1
Smpl no 21 1
2
Smpl no 21 2

Wavelength
225.00
260.00
210.10

Type
Standard

Type
Unknown
Unknown

Abs.

0.434
0.035
0.239

Conc
0.2000

Conc
0.2401
0.2409

Description

WL225.0
0.4392

Wgt.Factor
1.000

WL225.0

0.5273
0.5290

Comments
1 ad 10 5 ad 10

Comments
1 ad 10 5 ad 10
1 ad 10 5 ad 10

Universitas Sumatera Utara

31

Lampiran 2
Hasil Spektrofotometri Ultraviolet
Lampiran 3
Perhitungan Hasil Kromatografi Lapis Tipis
Rumus:
Harga Rf

=

Harga Rf :
Baku Asam Benzoat =
= 0,57
Sampel Sirup

=
= 0,52

Universitas Sumatera Utara

32

Lampiran 4
Perhitungan Kadar Asam Benzoat dalam Sirup Multivitamin
Kadar Asam Benzoat 1
Data
Au

: 0,5273

Ab

: 0,4392

Kb

: 2 mg/ml

Vtb

: 50 µl

Vtu

: 200 µl

Veu

: 5 ml

B

: 25046,5 mg

F

:1

Kadar Baku

: 99,94%

Kadar asam benzoat dapat dihitung:
Kadar =

Kadar =

x Kb

x2

x

x F x Kadar Baku

x

x 1 x 99,94%

Kadar = 0,01197%

Universitas Sumatera Utara