Karakterisasi Simplisia dan Formulasi Ekstrak Etanol Rumput Laut Merah (Eucheuma spinosum) sebagai Krim Anti-Aging
Lampiran 1. Hasil identifikasi rumput Laut Merah
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Lampiran 2. Gambar rumput laut merah
Gambar rumput laut merah segar
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Lampiran 3. Gambar Simplisia dan Serbuk Rumput laut merah
A
B
Keterangan:
A: Gambar simplisia Rumput laut merah
B: Gambar serbuk rumput laut merah
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Lampiran 4. Gambar mikroskopik serbuk simplisia rumput laut merah
1
2
Gambar hasil mikroskopik serbuk simplisia rumput laut merah
Keterangan:
1. Parenkim berisi pigmen merah
2. Parenkim bagian pinggir
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Lampiran 5. Bagan alur pembuatan ekstrak etanol rumput laut merah
Rumput laut merah
Dibersihkan
Dicuci dari pengotornya hingga bersih, tiriskan
Ditimbang berat basah
Diangin-anginkan terlebih dahulu
Dikeringkan dalam lemari pengering
Simplisia
rumput laut
merah
Ditimbang berat keringnya
Diperiksa organoleptis dan makroskopis
(bentuk, warna, dan tekstur)
Dihaluskan dengan blender
Serbuk rumput
laut merah
Karakterisasi
Serbuk Simplisia
Penetapan Kadar Air
Penetapan Kadar Sari Larut Air
Penetapan Kadar Sari Larut Etanol
Penetapan Kadar Abu Total
Penetapan Kadar Abu Tidak Larut
Asam
Direndam dengan pelarut etanol
96% selama 3 jam
Dipindahkan ke dalam perkolator
Didiamkan selama 24 jam
Dibuka kran dan dibiarkan ekstrak
menetes dari perkolator
Perkolat
Diuapkan
dengan
rotary
evaporator pada suhu 40-50C
Dipekatkan di atas penangas air
Ekstrak rumput
laut merah
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Lampiran 6. Bagan pembuatan krim ekstrak rumput laut merah
Bahan-bahan
ditimbang
Fase minyak:
Asam stearat
Setil alkohol
Fase air:
Propilen glikol
Sorbitol
Trietanolamin
Nipagin
Dimasukkan ke dalam
cawan penguap
Dilarutkan dalam air
panas pada suhu 70-75oC
Dilebur di atas penangas air
pada suhu 70-75oC
Fase minyak + fase air
Ekstrak rumput
laut merah
Digerus konstan di
dalam lumpang panas
hingga terbentuk massa
krim
Ditimbang masingmasing konsentrasi
Dimasukkan ke dalam
lumpang
Massa I
(Dasar krim)
Massa II
Ditambahkan dasar krim sedikit demi sedikit
ke dalam massa II
Digerus hingga homogen
Ditambahkan parfum
Digerus kembali hingga homogen
Krim ekstrak rumput laut
merah
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Lampiran 7. Hasil perhitungan karakterisasi simplisia rumput laut merah
7.1
Penetapan kadar air
Volume air (mL)
x 100%
Kadar air =
Berat sampel (g)
1. Berat sampel
= 5,0150 g
Volume air
= 1,85 mL
Kadar air
=
1,85
x 100% = 9,99%
5,015
2. Berat sampel
= 5,0102 g
Volume air
= 0,47 mL
Kadar air
=
0,47
5,0102
x 100% = 9,38%
3. Berat sampel
= 5,0263 g
Volume air
= 0,45 mL
Kadar air
=
Kadar air rata-rata =
0,45
x 100% = 8,95%
5,0263
9,99%+9,38%+8,95%
3
= 9,43%
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Lampiran 7. (Lanjutan)
7.2
Penetapan kadar sair larut air
Berat sari air (g) 100
x
x100%
Kadar sari larut air =
Berat sampel (g) 20
1. Berat sampel
= 5,0008 g
Berat sari air
= 0,2494 g
Kadar sari larut air
=
0,2494 100
x
x100% = 24,93%
5,0003 20
2. Berat sampel
= 5,0984 g
Berat sari air
= 0,2697 g
Kadar sari larut air
=
0,2697
5,0984
x
3. Berat sampel
= 5,0005 g
Berat sari air
= 0,2709 g
Kadar sari larut air
=
Kadar sari larut air rata-rata =
100
x100% = 26,44%
20
0,2709 100
x
x100% = 27,08%
5,0005 20
24,93+26,44+27,08
3
= 26,15%
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Lampiran 7. (Lanjutan)
7.3
Penetapan kadar sair larut etanol
Berat sari etanol (g) 100
x
x100%
Kadar sari larut etanol =
20
Berat sampel (g)
1. Berat sampel
= 5,0909 g
Berat sari etanol
= 0,0254 g
Kadar sari larut etanol
=
2. Berat sampel
0,0254 100
x
x100% = 2,49%
5,0909 20
= 5,0005 g
Berat sari etanol
= 0,0212 g
Kadar sari larut etanol
=
3. Berat sampel
0,0212
5,0005
x
100
x100% = 2,11%
20
= 5,0708 g
Berat sari etanol
= 0,0229 g
Kadar sari larut etanol
=
Kadar sari larut etanol rata-rata
=
0,0229 100
x
x100% = 2,25%
5,0708 20
2,49+2,11+2,25
3
= 2,29%
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Lampiran 7. (Lanjutan)
7.4
Penetapan kadar abu total
Berat abu (g)
x 100%
Kadar abu total =
Berat sampel (g)
1. Berat sampel
= 2,0230 g
Berat abu
= 0,3301 g
Kadar abu total
=
0,3301
x 100% = 16,31%
0,0230
2. Berat sampel
= 2,0505 g
Berat abu
= 0,3408 g
Kadar abu total
=
0,3408
2,0505
x 100% = 16,62%
3. Berat sampel
= 2,0255 g
Berat abu
= 0,3415 g
Kadar abu total
=
Kadar abu total rata-rata
=
0,3415
x 100% = 16,86%
2,0255
16,31+16,62+16,86
3
= 16,59%
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Lampiran 7. (Lanjutan)
7.5
Penetapan kadar abu tidak larut asam
Berat abu (g)
x 100%
Kadar abu tidak larut asam =
Berat sampel (g)
1. Berat sampel
= 2,0230 g
Berat abu
= 0,0321 g
Kadar abu tidak larut asam
=
0,0321
x 100% = 1,58%
2,0230
2. Berat sampel
= 2,0505 g
Berat abu
= 0,0228 g
Kadar abu tidak larut asam
=
0,0228
2,0505
x 100% = 1.11%
3. Berat sampel
= 2,0253 g
Berat abu
= 0,0394 g
Kadar abu tidak larut asam
=
Kadar abu tidak larut asam rata-rata =
0,0394
x 100% = 1,94%
2,0253
1,58+1,11+1,94
3
= 1,54%
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Lampiran 8. Gambar alat-alat penelitian
A
Q
B
Q
C
Q
D
Q
Keterangan: A: Neraca analitis (Dickson)
B: pH meter (Exact Instrument)
C: Moisture checker (Aramo Huvis)
D: Skin analyzer (Aramo Huvis)
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Lampiran 9. Gambar sediaan krim setelah dibuat dan setelah disimpan selama
90 hari dalam suhu kamar
A
B
Q
Keterangan: A: Sediaan krim setelah dibuat
B: Sediaan krim setelah disimpan selama 90 hari pada suhu kamar
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Lampiran 10. Gambar hasil uji homogenitas dan tipe emulsi sediaan krim
A
Q
B
Q
Keterangan: A: Hasil uji homogenitas
B: Hasil penentuan tipe emulsi
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Lampiran 11. Contoh surat pernyataan sukarelawan
SURAT PERNYATAAN PERSETUJUAN IKUT SERTA
DALAM PENELITIAN
Saya yang bertanda tangan di bawah ini
Nama lengkap : Afrina Panggabean
Umur
: 25 tahun
Alamat
: Jl. Parang 4 Gg Purba Dalam no 8 Medan
Telah mendapat penjelasan secukupnya bahwa saya bersedia untuk
menjadi sukarelawan untuk uji krim ekstrak Rumput Laut Merah terhadap kulit
sebagai sediaan krim anti-aging. Setelah mendapat penjelasan secukupnya tentang
manfaat penelitian ini dan efek sampingnya, maka saya menyatakan SETUJU
untuk ikut serta dalam penelitian dari Tessya Yolanda Tobing dengan judul
“FORMULASI KRIM EKSTRAK RUMPUT LAUT MERAH (Eucheuma
spinosum.) SEBAGAI ANTI-AGING, sebagai usaha untuk mengetahui apakah
sediaan krim anti-aging dari ekstrak rumput laut merah mampu atau tidak dalam
memulihkan kulit yang telah mengalami penuaan. Saya menyatakan sukarela dan
bersedia untuk mengikuti prosedur penelitian yang telah ditetapkan.
Persetujuan ini saya buat dengan penuh kesadaran dan tanpa paksaan
dari pihak manapun. Demikian surat pernyataan ini dibuat untuk dapat
dipergunakan sebagaimana mestinya.
Medan,
April 2016
Peneliti,
Sukarelawan
Tessya Yolanda Tobing
Afrina Panggabean
NIM 131524074
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Lampiran 12. Contoh hasil pengukuran menggunakan alat skin analyzer pada
kulit punggung tangan sukarelawan
Hasil pengukuran kadar air (moisture)
Kondisi awal
Pemakaian minggu I
Pemakaian minggu II
Pemakaian minggu III
Pemakaian minggu IV
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Lampiran 12. (Lanjutan)
Hasil pengukuran kehalusan/pori (evenness/pore)
Kondisi awal
Pemakaian minggu I
Pemakaian minggu II
\
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Lampiran 12. (Lanjutan)
Pemakaian minggu III
Pemakaian minggu IV
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Lampiran 12. (Lanjutan)
Hasil pengukuran noda (spot)
Kondisi awal
Pemakaian minggu I
Pemakaian minggu II
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Lampiran 12. (Lanjutan)
Pemakaian minggu III
Pemakaian minggu IV
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Lampiran 12. (Lanjutan)
Hasil pengukuran keriput (wrinkle)
Kondisi awal
V
Pemakaian minggu I
Pemakaian minggu III
Pemakaian minggu II
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Lampiran 12. (Lanjutan)
Pemakaian minggu III
Pemakaian minggu IV
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Lampiran 13. Data hasil uji statistik
Kadar air (Moisture)
One-Sample Kolmogorov-Smirnov Test
Minggu_awa
l
Minggu_1 Minggu_2 Minggu_3 Minggu_4
N
Normal
Parametersa,b
Mean
Std.
Deviation
15
15
15
15
15
27,1333
29,9333
31,7333
33,0667
34,4000
1,12546
1,48645
2,73774
3,08143
3,31231
Most Extreme
Absolute
,253
,149
,128
,158
,119
Differences
Positive
,154
,149
,128
,132
,108
Negative
-,253
-,118
-,086
-,158
-,119
Test Statistic
,253
,149
,128
,158
,119
Asymp. Sig. (2-tailed)
,011c
,200c,d
,200c,d
,200c,d
,200c,d
F
Sig.
a. Test distribution is Normal.
b. Calculated from data.
c. Lilliefors Significance Correction.
d. This is a lower bound of the true significance.
ANOVA
Sum of
Squares
Minggu_awal
df
Mean Square
Between Groups
8,400
4
2,100
Within Groups
9,333
10
,933
14
Total
Between Groups
17,733
Minggu_1
24,933
4
6,233
6,000
30,933
10
14
,600
Minggu_2
Within Groups
Total
Between Groups
94,933
4
23,733
10,000
104,933
10
14
1,000
Minggu_3
Within Groups
Total
Between Groups
121,600
4
30,400
11,333
132,933
10
14
1,133
Minggu_4
Within Groups
Total
Between Groups
144,933
4
36,233
8,667
10
,867
153,600
14
Within Groups
Total
2,250
,136
10,389
,001
23,733
,000
26,824
,000
41,808
,000
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Homogeneous Subsets
Minggu_awal
Tukey HSDa
Subset for
alpha = 0.05
kelompok
N
1
formula 4
3
25,6667
formula 2
3
27,3333
formula 3
3
27,3333
Formula
3
27,6667
0
formula 1
3
27,6667
Sig.
,158
Means for groups in homogeneous
subsets are displayed.
a. Uses Harmonic Mean Sample
Size = 3,000.
Minggu_1
a
Tukey HSD
Subset for alpha = 0.05
Kelompok
N
1
2
3
Formula 0
3
28,3333
formula 1
3
29,0000
29,0000
formula 2
3
29,6667
29,6667
formula 3
3
30,6667
30,6667
formula 4
3
32,0000
Sig.
,288
,136
,288
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_2
Tukey HSDa
Subset for alpha = 0.05
2
3
kelompok
N
1
Formula 0
3
28,3333
formula 1
3
30,0000
30,0000
formula 2
3
31,6667
31,6667
formula 3
3
33,0000
formula 4
3
Sig.
,314
,314
,511
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
4
33,0000
35,6667
,052
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Lampiran 13. (Lanjutan)
Minggu_3
Tukey HSDa
Subset for alpha = 0.05
kelompok
N
1
2
3
Formula 0
3
formula 1
3
31,6667
formula 2
3
33,0000
formula 3
3
formula 4
3
Sig.
4
28,6667
33,0000
35,0000
35,0000
37,0000
1,000
,566
,221
,221
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_4
Tukey HSDa
Subset for alpha = 0.05
kelompok
N
1
2
3
Formula 0
3
formula 1
3
33,0000
formula 2
3
34,6667
formula 3
3
formula 4
3
Sig.
4
29,3333
34,6667
36,6667
36,6667
38,3333
1,000
,257
,137
,257
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
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Lampiran 13. (Lanjutan)
Kehalusan (Evennes)
One-Sample Kolmogorov-Smirnov Test
N
Normal Parametersa,b
Most Extreme
Differences
Mean
Std.
Deviation
Absolute
Minggu_A Minggu_ Minggu_ Minggu_ Minggu_
wal
1
2
3
4
15
15
15
15
15
40,2667 36,2000 34,0667 31,0667 28,4667
1,43759
2,11119
3,03472
4,52717
5,57887
,174
,174
-,160
,174
,204
,204
-,085
,204
,242
,242
-,115
,242
,173
,173
-,129
,173
,137
,137
-,137
,137
,200c,d
,092c
,018c
,200c,d
,200c,d
Positive
Negative
Test Statistic
Asymp. Sig. (2-tailed)
a. Test distribution is Normal.
b. Calculated from data.
c. Lilliefors Significance Correction.
d. This is a lower bound of the true significance.
ANOVA
Sum of
Squares
Minggu_Aw Between Groups
al
df
Mean Square
14,933
4
3,733
14,000
28,933
10
14
1,400
Minggu_1
Within Groups
Total
Between Groups
41,733
4
10,433
20,667
62,400
10
14
2,067
Minggu_2
Within Groups
Total
Between Groups
107,600
4
26,900
21,333
128,933
10
14
2,133
Minggu_3
Within Groups
Total
Between Groups
261,600
4
65,400
25,333
286,933
10
14
2,533
Minggu_4
Within Groups
Total
Between Groups
401,733
4
100,433
Within Groups
Total
34,000
435,733
10
14
3,400
F
Sig.
2,667
,095
5,048
,017
12,609
,001
25,816
,000
29,539
,000
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Lampiran 13. (Lanjutan)
Homogeneous Subsets
Minggu_Awal
a
Tukey HSD
Subset for alpha
= 0.05
Kelompok
N
1
Formula 2
3
39,0000
Formula 3
3
39,6667
Formula 1
3
40,3333
Formula 4
3
40,3333
Formula 0
3
42,0000
Sig.
,067
Means for groups in homogeneous subsets
are displayed.
a. Uses Harmonic Mean Sample Size =
3,000.
Minggu_1
Tukey HSDa
Subset for alpha =
0.05
1
2
kelompok
N
Formula
3 34,3333
4
Formula
3 35,6667 35,6667
2
Formula
3 35,6667 35,6667
3
Formula
3 36,0000 36,0000
1
Formula
3
39,3333
0
Sig.
,630
,065
Means for groups in homogeneous subsets
are displayed.
a. Uses Harmonic Mean Sample Size =
3,000.
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Lampiran 13. (Lanjutan)
Minggu_2
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
Formula 4
3
31,6667
Formula 3
3
32,0000
Formula 2
3
33,0000
Formula 1
3
34,6667
Formula 0
3
39,0000
Sig.
,163
1,000
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_3
Tukey HSDa
kelompok
Formula 4
Subset for alpha = 0.05
1
2
3
N
3
25,6667
Formula 3
3
29,0000
29,0000
Formula 2
3
29,6667
29,6667
Formula 1
3
Formula 0
3
33,0000
38,0000
Sig.
,070
,070
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_4
Tukey HSDa
Subset for alpha = 0.05
kelompok
N
1
2
3
Formula 4
3 22,0000
Formula 3
3 25,6667 25,6667
Formula 2
3
27,0000
Formula 1
3
30,3333
Formula 0
3
37,3333
Sig.
,183
,067
1,000
a. Uses Harmonic Mean Sample Size = 3,000.
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Lampiran 13. (Lanjutan)
Pori (Pore)
One-Sample Kolmogorov-Smirnov Test
Minggu_A
wal
N
Normal Parameters
a,b
Mean
Std.
Deviation
Absolute
Most Extreme
Differences
Minggu_ Minggu_ Minggu_ Minggu_
1
2
3
4
15
15
15
15
15
34,8667
31,5333
28,1333
25,0667
22,1333
2,94877
5,50151
6,91651
7,15608
8,19291
,251
,338
,292
,186
,124
,150
-,251
,264
-,338
,160
-,292
,139
-,186
,092
-,124
,251
,338
,292
,186
,124
c
c
c
c
,200c,d
Positive
Negative
Test Statistic
Asymp. Sig. (2-tailed)
,012
,000
,001
,170
a. Test distribution is Normal.
b. Calculated from data.
c. Lilliefors Significance Correction.
d. This is a lower bound of the true significance.
ANOVA
Sum of
Squares
df
Mean Square
Minggu_Aw Between Groups
65,067
4
16,267
al
56,667
10
5,667
Total
121,733
14
Between Groups
368,400
4
92,100
55,333
10
5,533
Total
423,733
14
Between Groups
659,067
4
164,767
10,667
10
1,067
Total
669,733
14
Between Groups
706,267
4
176,567
10,667
10
1,067
Total
716,933
14
Between Groups
925,067
4
231,267
14,667
10
1,467
939,733
14
Minggu_1
Within Groups
Within Groups
Minggu_2
Within Groups
Minggu_3
Within Groups
Minggu_4
Within Groups
Total
F
Sig.
2,871
,080
16,645
,000
154,469
,000
165,531
,000
157,682
,000
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Lampiran 13. (Lanjutan)
Homogeneous Subsets
Minggu_Awal
a
Tukey HSD
Subset for alpha
= 0.05
Kelompok
N
1
Formula 4
3
31,3333
Formula 0
3
34,6667
Formula 1
3
34,6667
Formula 2
3
36,0000
Formula 3
3
37,6667
Sig.
,053
Means for groups in homogeneous subsets
are displayed.
a. Uses Harmonic Mean Sample Size =
3,000.
Minggu_1
a
Tukey HSD
Subset for alpha =
0.05
1
2
Kelompo
k
N
Formula
3 21,6667
4
Formula
3
33,3333
1
Formula
3
33,6667
2
Formula
3
34,3333
3
Formula
3
34,6667
0
Sig.
1,000
,953
Means for groups in homogeneous subsets
are displayed.
a. Uses Harmonic Mean Sample Size =
3,000.
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Lampiran 13. (Lanjutan)
Minggu_2
Tukey HSDa
Subset for alpha = 0.05
2
3
Kelompok
N
1
Formula 4
3
15,3333
Formula 2
3
29,3333
Formula 3
3
29,6667
29,6667
Formula 1
3
32,3333
Formula 0
3
Sig.
1,000
,994
,061
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
4
32,3333
34,0000
,342
Minggu_3
Tukey
HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Formula 4
3
Formula 3
3
24,0000
Formula 2
3
26,3333
Formula 1
3
Formula 0
3
4
12,6667
29,6667
32,6667
Sig.
1,000
,112
1,000
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_4
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
Formula 4
3
Formula 3
3
Formula 2
3
Formula 1
3
Formula 0
3
Sig.
2
3
4
5
9,3333
19,0000
22,3333
27,6667
32,3333
1,000
1,000
1,000
1,000
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
86
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Noda ( Spot)
One-Sample Kolmogorov-Smirnov Test
N
Normal Parametersa,b
Most Extreme
Differences
Mean
Std.
Deviation
Absolute
Positive
Negative
Minggu_A Minggu_ Minggu_ Minggu_ Minggu_
wal
1
2
3
4
15
15
15
15
15
42,6000 39,4000 36,0000 32,5333 29,4667
1,91982
2,35433
4,65986
7,70776
8,64264
,217
,217
-,111
,217
,055c
,201
,133
-,201
,201
,107c
,185
,101
-,185
,185
,178c
,207
,114
-,207
,207
,084c
,167
,111
-,167
,167
,200c,d
F
Sig.
Test Statistic
Asymp. Sig. (2-tailed)
a. Test distribution is Normal.
b. Calculated from data.
c. Lilliefors Significance Correction.
d. This is a lower bound of the true significance.
ANOVA
Sum of
Squares
Minggu_A
wal
Minggu_1
Minggu_2
Minggu_3
Minggu_4
Between
Groups
Mean
Square
df
30,267
4
7,567
Within Groups
Total
Between
Groups
21,333
51,600
10
14
2,133
52,933
4
13,233
Within Groups
Total
Between
Groups
24,667
77,600
10
14
2,467
274,000
4
68,500
Within Groups
Total
Between
Groups
30,000
304,000
10
14
3,000
796,400
4
199,100
Within Groups
Total
Between
Groups
35,333
831,733
10
14
3,533
1005,733
4
251,433
Within Groups
Total
40,000
1045,733
10
14
4,000
3,547
,048
5,365
,014
22,833
,000
56,349
,000
62,858
,000
87
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Homogeneous Subsets
Minggu_Awal
Tukey HSD
a
Subset for alpha = 0.05
Kelompok
N
1
2
Formula 1
3
41,0000
Formula 2
3
41,6667
41,6667
Formula 0
3
42,0000
42,0000
Formula 3
3
43,3333
43,3333
Formula 4
3
45,0000
Sig.
,350
,107
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_1
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
Formula 4
3
36,3333
Formula 3
3
38,6667
38,6667
Formula 2
3
39,6667
39,6667
Formula 1
3
40,3333
40,3333
Formula 0
3
42,0000
Sig.
,065
,144
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_2
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Formula 4
3 28,3333
Formula 3
3
36,0000
Formula 2
3
36,3333
Formula 1
3
38,0000 38,0000
Formula 0
3
41,3333
Sig.
1,000
,633
,204
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
88
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Lampiran 13. (Lanjutan)
Minggu_3
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Formula 4
3
19,3333
Formula 3
3
30,6667
Formula 2
3
35,6667
35,6667
Formula 1
3
36,6667
Formula 0
3
40,3333
Sig.
1,000
,053
,074
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_4
Tukey HSDa
Subset for alpha = 0.05
Kelompo
k
N
1
2
3
4
Formula
3 15,0000
4
Formula
3
27,3333
3
Formula
3
31,3333 31,3333
2
Formula
3
34,6667 34,6667
1
Formula
3
39,0000
0
Sig.
1,000
,179
,314
,133
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
89
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Lampiran 13. (Lanjutan)
Keriput (Wrinkle)
One-Sample Kolmogorov-Smirnov Test
Minggu_A
wal
N
Minggu_ Minggu_ MInggu_ Minggu_
1
2
3
4
15
15
15
15
15
28,4000
26,4667
24,8000
22,7333
20,6000
,98561
1,68466
2,59670
3,95450
5,34255
Test Statistic
,262
,205
-,262
,262
,176
,176
-,124
,176
,221
,221
-,112
,221
,174
,174
-,131
,174
,127
,127
-,117
,127
Asymp. Sig. (2-tailed)
,007c
,200c,d
,047c
,200c,d
,200c,d
F
Sig.
Normal Parametersa,b
Mean
Std.
Deviation
Absolute
Most Extreme
Differences
Positive
Negative
a. Test distribution is Normal.
b. Calculated from data.
c. Lilliefors Significance Correction.
d. This is a lower bound of the true significance.
ANOVA
Sum of
Squares
Minggu_Aw Between Groups
al
Minggu_1
Minggu_2
MInggu_3
Mean Square
3,600
4
,900
Within Groups
10,000
10
1,000
Total
13,600
14
Between Groups
28,400
4
7,100
Within Groups
11,333
10
1,133
Total
39,733
14
Between Groups
84,400
4
21,100
Within Groups
10,000
10
1,000
Total
94,400
14
204,933
4
51,233
14,000
10
1,400
Total
218,933
14
Between Groups
388,267
4
97,067
11,333
10
1,133
399,600
14
Between Groups
Within Groups
Minggu_4
df
Within Groups
Total
,900
,499
6,265
,009
21,100
,000
36,595
,000
85,647
,000
90
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Homogeneous Subsets
Minggu_Awal
a
Tukey HSD
Subset for alpha =
0.05
Kelompok
N
1
Formula 1
3
27,6667
Formula 3
3
28,0000
Formula 2
3
28,6667
Formula 4
3
28,6667
Formula 0
3
29,0000
Sig.
,511
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_1
a
Tukey HSD
Kelompok
Formula 4
Formula 3
Formula 2
Formula 1
Formula 0
Sig.
Subset for alpha = 0.05
1
2
N
3
3
3
3
3
25,0000
25,6667
26,0000
26,6667
,368
26,6667
29,0000
,127
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
91
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Lampiran 13. (Lanjutan)
Minggu_2
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Formula 4
3
22,0000
Formula 3
3
23,3333
23,3333
Formula 2
3
24,3333
24,3333
Formula 1
3
Formula 0
3
25,3333
29,0000
Sig.
,098
,179
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MInggu_3
Tukey HSDa
Kelompok
Formula 4
N
Subset for alpha = 0.05
2
1
3
3
17,6667
Formula 3
3
21,3333
Formula 2
3
22,0000
Formula 1
3
23,6667
Formula 0
3
29,0000
Sig.
1,000
,188
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_4
Tukey HSDa
Kelompok
Formula 4
N
1
3
Subset for alpha = 0.05
2
3
14,0000
Formula 3
3
17,3333
Formula 2
3
20,0000
Formula 1
3
Formula 0
3
Sig.
4
20,0000
22,6667
29,0000
1,000
,071
,071
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
92
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56
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Lampiran 2. Gambar rumput laut merah
Gambar rumput laut merah segar
57
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Lampiran 3. Gambar Simplisia dan Serbuk Rumput laut merah
A
B
Keterangan:
A: Gambar simplisia Rumput laut merah
B: Gambar serbuk rumput laut merah
58
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Lampiran 4. Gambar mikroskopik serbuk simplisia rumput laut merah
1
2
Gambar hasil mikroskopik serbuk simplisia rumput laut merah
Keterangan:
1. Parenkim berisi pigmen merah
2. Parenkim bagian pinggir
59
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Lampiran 5. Bagan alur pembuatan ekstrak etanol rumput laut merah
Rumput laut merah
Dibersihkan
Dicuci dari pengotornya hingga bersih, tiriskan
Ditimbang berat basah
Diangin-anginkan terlebih dahulu
Dikeringkan dalam lemari pengering
Simplisia
rumput laut
merah
Ditimbang berat keringnya
Diperiksa organoleptis dan makroskopis
(bentuk, warna, dan tekstur)
Dihaluskan dengan blender
Serbuk rumput
laut merah
Karakterisasi
Serbuk Simplisia
Penetapan Kadar Air
Penetapan Kadar Sari Larut Air
Penetapan Kadar Sari Larut Etanol
Penetapan Kadar Abu Total
Penetapan Kadar Abu Tidak Larut
Asam
Direndam dengan pelarut etanol
96% selama 3 jam
Dipindahkan ke dalam perkolator
Didiamkan selama 24 jam
Dibuka kran dan dibiarkan ekstrak
menetes dari perkolator
Perkolat
Diuapkan
dengan
rotary
evaporator pada suhu 40-50C
Dipekatkan di atas penangas air
Ekstrak rumput
laut merah
60
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Lampiran 6. Bagan pembuatan krim ekstrak rumput laut merah
Bahan-bahan
ditimbang
Fase minyak:
Asam stearat
Setil alkohol
Fase air:
Propilen glikol
Sorbitol
Trietanolamin
Nipagin
Dimasukkan ke dalam
cawan penguap
Dilarutkan dalam air
panas pada suhu 70-75oC
Dilebur di atas penangas air
pada suhu 70-75oC
Fase minyak + fase air
Ekstrak rumput
laut merah
Digerus konstan di
dalam lumpang panas
hingga terbentuk massa
krim
Ditimbang masingmasing konsentrasi
Dimasukkan ke dalam
lumpang
Massa I
(Dasar krim)
Massa II
Ditambahkan dasar krim sedikit demi sedikit
ke dalam massa II
Digerus hingga homogen
Ditambahkan parfum
Digerus kembali hingga homogen
Krim ekstrak rumput laut
merah
61
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Lampiran 7. Hasil perhitungan karakterisasi simplisia rumput laut merah
7.1
Penetapan kadar air
Volume air (mL)
x 100%
Kadar air =
Berat sampel (g)
1. Berat sampel
= 5,0150 g
Volume air
= 1,85 mL
Kadar air
=
1,85
x 100% = 9,99%
5,015
2. Berat sampel
= 5,0102 g
Volume air
= 0,47 mL
Kadar air
=
0,47
5,0102
x 100% = 9,38%
3. Berat sampel
= 5,0263 g
Volume air
= 0,45 mL
Kadar air
=
Kadar air rata-rata =
0,45
x 100% = 8,95%
5,0263
9,99%+9,38%+8,95%
3
= 9,43%
62
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Lampiran 7. (Lanjutan)
7.2
Penetapan kadar sair larut air
Berat sari air (g) 100
x
x100%
Kadar sari larut air =
Berat sampel (g) 20
1. Berat sampel
= 5,0008 g
Berat sari air
= 0,2494 g
Kadar sari larut air
=
0,2494 100
x
x100% = 24,93%
5,0003 20
2. Berat sampel
= 5,0984 g
Berat sari air
= 0,2697 g
Kadar sari larut air
=
0,2697
5,0984
x
3. Berat sampel
= 5,0005 g
Berat sari air
= 0,2709 g
Kadar sari larut air
=
Kadar sari larut air rata-rata =
100
x100% = 26,44%
20
0,2709 100
x
x100% = 27,08%
5,0005 20
24,93+26,44+27,08
3
= 26,15%
63
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Lampiran 7. (Lanjutan)
7.3
Penetapan kadar sair larut etanol
Berat sari etanol (g) 100
x
x100%
Kadar sari larut etanol =
20
Berat sampel (g)
1. Berat sampel
= 5,0909 g
Berat sari etanol
= 0,0254 g
Kadar sari larut etanol
=
2. Berat sampel
0,0254 100
x
x100% = 2,49%
5,0909 20
= 5,0005 g
Berat sari etanol
= 0,0212 g
Kadar sari larut etanol
=
3. Berat sampel
0,0212
5,0005
x
100
x100% = 2,11%
20
= 5,0708 g
Berat sari etanol
= 0,0229 g
Kadar sari larut etanol
=
Kadar sari larut etanol rata-rata
=
0,0229 100
x
x100% = 2,25%
5,0708 20
2,49+2,11+2,25
3
= 2,29%
64
Universitas Sumatera Utara
Lampiran 7. (Lanjutan)
7.4
Penetapan kadar abu total
Berat abu (g)
x 100%
Kadar abu total =
Berat sampel (g)
1. Berat sampel
= 2,0230 g
Berat abu
= 0,3301 g
Kadar abu total
=
0,3301
x 100% = 16,31%
0,0230
2. Berat sampel
= 2,0505 g
Berat abu
= 0,3408 g
Kadar abu total
=
0,3408
2,0505
x 100% = 16,62%
3. Berat sampel
= 2,0255 g
Berat abu
= 0,3415 g
Kadar abu total
=
Kadar abu total rata-rata
=
0,3415
x 100% = 16,86%
2,0255
16,31+16,62+16,86
3
= 16,59%
65
Universitas Sumatera Utara
Lampiran 7. (Lanjutan)
7.5
Penetapan kadar abu tidak larut asam
Berat abu (g)
x 100%
Kadar abu tidak larut asam =
Berat sampel (g)
1. Berat sampel
= 2,0230 g
Berat abu
= 0,0321 g
Kadar abu tidak larut asam
=
0,0321
x 100% = 1,58%
2,0230
2. Berat sampel
= 2,0505 g
Berat abu
= 0,0228 g
Kadar abu tidak larut asam
=
0,0228
2,0505
x 100% = 1.11%
3. Berat sampel
= 2,0253 g
Berat abu
= 0,0394 g
Kadar abu tidak larut asam
=
Kadar abu tidak larut asam rata-rata =
0,0394
x 100% = 1,94%
2,0253
1,58+1,11+1,94
3
= 1,54%
66
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Lampiran 8. Gambar alat-alat penelitian
A
Q
B
Q
C
Q
D
Q
Keterangan: A: Neraca analitis (Dickson)
B: pH meter (Exact Instrument)
C: Moisture checker (Aramo Huvis)
D: Skin analyzer (Aramo Huvis)
67
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Lampiran 9. Gambar sediaan krim setelah dibuat dan setelah disimpan selama
90 hari dalam suhu kamar
A
B
Q
Keterangan: A: Sediaan krim setelah dibuat
B: Sediaan krim setelah disimpan selama 90 hari pada suhu kamar
68
Universitas Sumatera Utara
Lampiran 10. Gambar hasil uji homogenitas dan tipe emulsi sediaan krim
A
Q
B
Q
Keterangan: A: Hasil uji homogenitas
B: Hasil penentuan tipe emulsi
69
Universitas Sumatera Utara
Lampiran 11. Contoh surat pernyataan sukarelawan
SURAT PERNYATAAN PERSETUJUAN IKUT SERTA
DALAM PENELITIAN
Saya yang bertanda tangan di bawah ini
Nama lengkap : Afrina Panggabean
Umur
: 25 tahun
Alamat
: Jl. Parang 4 Gg Purba Dalam no 8 Medan
Telah mendapat penjelasan secukupnya bahwa saya bersedia untuk
menjadi sukarelawan untuk uji krim ekstrak Rumput Laut Merah terhadap kulit
sebagai sediaan krim anti-aging. Setelah mendapat penjelasan secukupnya tentang
manfaat penelitian ini dan efek sampingnya, maka saya menyatakan SETUJU
untuk ikut serta dalam penelitian dari Tessya Yolanda Tobing dengan judul
“FORMULASI KRIM EKSTRAK RUMPUT LAUT MERAH (Eucheuma
spinosum.) SEBAGAI ANTI-AGING, sebagai usaha untuk mengetahui apakah
sediaan krim anti-aging dari ekstrak rumput laut merah mampu atau tidak dalam
memulihkan kulit yang telah mengalami penuaan. Saya menyatakan sukarela dan
bersedia untuk mengikuti prosedur penelitian yang telah ditetapkan.
Persetujuan ini saya buat dengan penuh kesadaran dan tanpa paksaan
dari pihak manapun. Demikian surat pernyataan ini dibuat untuk dapat
dipergunakan sebagaimana mestinya.
Medan,
April 2016
Peneliti,
Sukarelawan
Tessya Yolanda Tobing
Afrina Panggabean
NIM 131524074
70
Universitas Sumatera Utara
Lampiran 12. Contoh hasil pengukuran menggunakan alat skin analyzer pada
kulit punggung tangan sukarelawan
Hasil pengukuran kadar air (moisture)
Kondisi awal
Pemakaian minggu I
Pemakaian minggu II
Pemakaian minggu III
Pemakaian minggu IV
71
Universitas Sumatera Utara
Lampiran 12. (Lanjutan)
Hasil pengukuran kehalusan/pori (evenness/pore)
Kondisi awal
Pemakaian minggu I
Pemakaian minggu II
\
72
Universitas Sumatera Utara
Lampiran 12. (Lanjutan)
Pemakaian minggu III
Pemakaian minggu IV
73
Universitas Sumatera Utara
Lampiran 12. (Lanjutan)
Hasil pengukuran noda (spot)
Kondisi awal
Pemakaian minggu I
Pemakaian minggu II
74
Universitas Sumatera Utara
Lampiran 12. (Lanjutan)
Pemakaian minggu III
Pemakaian minggu IV
75
Universitas Sumatera Utara
Lampiran 12. (Lanjutan)
Hasil pengukuran keriput (wrinkle)
Kondisi awal
V
Pemakaian minggu I
Pemakaian minggu III
Pemakaian minggu II
76
Universitas Sumatera Utara
Lampiran 12. (Lanjutan)
Pemakaian minggu III
Pemakaian minggu IV
77
Universitas Sumatera Utara
Lampiran 13. Data hasil uji statistik
Kadar air (Moisture)
One-Sample Kolmogorov-Smirnov Test
Minggu_awa
l
Minggu_1 Minggu_2 Minggu_3 Minggu_4
N
Normal
Parametersa,b
Mean
Std.
Deviation
15
15
15
15
15
27,1333
29,9333
31,7333
33,0667
34,4000
1,12546
1,48645
2,73774
3,08143
3,31231
Most Extreme
Absolute
,253
,149
,128
,158
,119
Differences
Positive
,154
,149
,128
,132
,108
Negative
-,253
-,118
-,086
-,158
-,119
Test Statistic
,253
,149
,128
,158
,119
Asymp. Sig. (2-tailed)
,011c
,200c,d
,200c,d
,200c,d
,200c,d
F
Sig.
a. Test distribution is Normal.
b. Calculated from data.
c. Lilliefors Significance Correction.
d. This is a lower bound of the true significance.
ANOVA
Sum of
Squares
Minggu_awal
df
Mean Square
Between Groups
8,400
4
2,100
Within Groups
9,333
10
,933
14
Total
Between Groups
17,733
Minggu_1
24,933
4
6,233
6,000
30,933
10
14
,600
Minggu_2
Within Groups
Total
Between Groups
94,933
4
23,733
10,000
104,933
10
14
1,000
Minggu_3
Within Groups
Total
Between Groups
121,600
4
30,400
11,333
132,933
10
14
1,133
Minggu_4
Within Groups
Total
Between Groups
144,933
4
36,233
8,667
10
,867
153,600
14
Within Groups
Total
2,250
,136
10,389
,001
23,733
,000
26,824
,000
41,808
,000
78
Universitas Sumatera Utara
Homogeneous Subsets
Minggu_awal
Tukey HSDa
Subset for
alpha = 0.05
kelompok
N
1
formula 4
3
25,6667
formula 2
3
27,3333
formula 3
3
27,3333
Formula
3
27,6667
0
formula 1
3
27,6667
Sig.
,158
Means for groups in homogeneous
subsets are displayed.
a. Uses Harmonic Mean Sample
Size = 3,000.
Minggu_1
a
Tukey HSD
Subset for alpha = 0.05
Kelompok
N
1
2
3
Formula 0
3
28,3333
formula 1
3
29,0000
29,0000
formula 2
3
29,6667
29,6667
formula 3
3
30,6667
30,6667
formula 4
3
32,0000
Sig.
,288
,136
,288
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_2
Tukey HSDa
Subset for alpha = 0.05
2
3
kelompok
N
1
Formula 0
3
28,3333
formula 1
3
30,0000
30,0000
formula 2
3
31,6667
31,6667
formula 3
3
33,0000
formula 4
3
Sig.
,314
,314
,511
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
4
33,0000
35,6667
,052
79
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Minggu_3
Tukey HSDa
Subset for alpha = 0.05
kelompok
N
1
2
3
Formula 0
3
formula 1
3
31,6667
formula 2
3
33,0000
formula 3
3
formula 4
3
Sig.
4
28,6667
33,0000
35,0000
35,0000
37,0000
1,000
,566
,221
,221
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_4
Tukey HSDa
Subset for alpha = 0.05
kelompok
N
1
2
3
Formula 0
3
formula 1
3
33,0000
formula 2
3
34,6667
formula 3
3
formula 4
3
Sig.
4
29,3333
34,6667
36,6667
36,6667
38,3333
1,000
,257
,137
,257
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
80
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Kehalusan (Evennes)
One-Sample Kolmogorov-Smirnov Test
N
Normal Parametersa,b
Most Extreme
Differences
Mean
Std.
Deviation
Absolute
Minggu_A Minggu_ Minggu_ Minggu_ Minggu_
wal
1
2
3
4
15
15
15
15
15
40,2667 36,2000 34,0667 31,0667 28,4667
1,43759
2,11119
3,03472
4,52717
5,57887
,174
,174
-,160
,174
,204
,204
-,085
,204
,242
,242
-,115
,242
,173
,173
-,129
,173
,137
,137
-,137
,137
,200c,d
,092c
,018c
,200c,d
,200c,d
Positive
Negative
Test Statistic
Asymp. Sig. (2-tailed)
a. Test distribution is Normal.
b. Calculated from data.
c. Lilliefors Significance Correction.
d. This is a lower bound of the true significance.
ANOVA
Sum of
Squares
Minggu_Aw Between Groups
al
df
Mean Square
14,933
4
3,733
14,000
28,933
10
14
1,400
Minggu_1
Within Groups
Total
Between Groups
41,733
4
10,433
20,667
62,400
10
14
2,067
Minggu_2
Within Groups
Total
Between Groups
107,600
4
26,900
21,333
128,933
10
14
2,133
Minggu_3
Within Groups
Total
Between Groups
261,600
4
65,400
25,333
286,933
10
14
2,533
Minggu_4
Within Groups
Total
Between Groups
401,733
4
100,433
Within Groups
Total
34,000
435,733
10
14
3,400
F
Sig.
2,667
,095
5,048
,017
12,609
,001
25,816
,000
29,539
,000
81
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Homogeneous Subsets
Minggu_Awal
a
Tukey HSD
Subset for alpha
= 0.05
Kelompok
N
1
Formula 2
3
39,0000
Formula 3
3
39,6667
Formula 1
3
40,3333
Formula 4
3
40,3333
Formula 0
3
42,0000
Sig.
,067
Means for groups in homogeneous subsets
are displayed.
a. Uses Harmonic Mean Sample Size =
3,000.
Minggu_1
Tukey HSDa
Subset for alpha =
0.05
1
2
kelompok
N
Formula
3 34,3333
4
Formula
3 35,6667 35,6667
2
Formula
3 35,6667 35,6667
3
Formula
3 36,0000 36,0000
1
Formula
3
39,3333
0
Sig.
,630
,065
Means for groups in homogeneous subsets
are displayed.
a. Uses Harmonic Mean Sample Size =
3,000.
82
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Minggu_2
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
Formula 4
3
31,6667
Formula 3
3
32,0000
Formula 2
3
33,0000
Formula 1
3
34,6667
Formula 0
3
39,0000
Sig.
,163
1,000
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_3
Tukey HSDa
kelompok
Formula 4
Subset for alpha = 0.05
1
2
3
N
3
25,6667
Formula 3
3
29,0000
29,0000
Formula 2
3
29,6667
29,6667
Formula 1
3
Formula 0
3
33,0000
38,0000
Sig.
,070
,070
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_4
Tukey HSDa
Subset for alpha = 0.05
kelompok
N
1
2
3
Formula 4
3 22,0000
Formula 3
3 25,6667 25,6667
Formula 2
3
27,0000
Formula 1
3
30,3333
Formula 0
3
37,3333
Sig.
,183
,067
1,000
a. Uses Harmonic Mean Sample Size = 3,000.
83
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Pori (Pore)
One-Sample Kolmogorov-Smirnov Test
Minggu_A
wal
N
Normal Parameters
a,b
Mean
Std.
Deviation
Absolute
Most Extreme
Differences
Minggu_ Minggu_ Minggu_ Minggu_
1
2
3
4
15
15
15
15
15
34,8667
31,5333
28,1333
25,0667
22,1333
2,94877
5,50151
6,91651
7,15608
8,19291
,251
,338
,292
,186
,124
,150
-,251
,264
-,338
,160
-,292
,139
-,186
,092
-,124
,251
,338
,292
,186
,124
c
c
c
c
,200c,d
Positive
Negative
Test Statistic
Asymp. Sig. (2-tailed)
,012
,000
,001
,170
a. Test distribution is Normal.
b. Calculated from data.
c. Lilliefors Significance Correction.
d. This is a lower bound of the true significance.
ANOVA
Sum of
Squares
df
Mean Square
Minggu_Aw Between Groups
65,067
4
16,267
al
56,667
10
5,667
Total
121,733
14
Between Groups
368,400
4
92,100
55,333
10
5,533
Total
423,733
14
Between Groups
659,067
4
164,767
10,667
10
1,067
Total
669,733
14
Between Groups
706,267
4
176,567
10,667
10
1,067
Total
716,933
14
Between Groups
925,067
4
231,267
14,667
10
1,467
939,733
14
Minggu_1
Within Groups
Within Groups
Minggu_2
Within Groups
Minggu_3
Within Groups
Minggu_4
Within Groups
Total
F
Sig.
2,871
,080
16,645
,000
154,469
,000
165,531
,000
157,682
,000
84
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Homogeneous Subsets
Minggu_Awal
a
Tukey HSD
Subset for alpha
= 0.05
Kelompok
N
1
Formula 4
3
31,3333
Formula 0
3
34,6667
Formula 1
3
34,6667
Formula 2
3
36,0000
Formula 3
3
37,6667
Sig.
,053
Means for groups in homogeneous subsets
are displayed.
a. Uses Harmonic Mean Sample Size =
3,000.
Minggu_1
a
Tukey HSD
Subset for alpha =
0.05
1
2
Kelompo
k
N
Formula
3 21,6667
4
Formula
3
33,3333
1
Formula
3
33,6667
2
Formula
3
34,3333
3
Formula
3
34,6667
0
Sig.
1,000
,953
Means for groups in homogeneous subsets
are displayed.
a. Uses Harmonic Mean Sample Size =
3,000.
85
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Minggu_2
Tukey HSDa
Subset for alpha = 0.05
2
3
Kelompok
N
1
Formula 4
3
15,3333
Formula 2
3
29,3333
Formula 3
3
29,6667
29,6667
Formula 1
3
32,3333
Formula 0
3
Sig.
1,000
,994
,061
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
4
32,3333
34,0000
,342
Minggu_3
Tukey
HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Formula 4
3
Formula 3
3
24,0000
Formula 2
3
26,3333
Formula 1
3
Formula 0
3
4
12,6667
29,6667
32,6667
Sig.
1,000
,112
1,000
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_4
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
Formula 4
3
Formula 3
3
Formula 2
3
Formula 1
3
Formula 0
3
Sig.
2
3
4
5
9,3333
19,0000
22,3333
27,6667
32,3333
1,000
1,000
1,000
1,000
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
86
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Noda ( Spot)
One-Sample Kolmogorov-Smirnov Test
N
Normal Parametersa,b
Most Extreme
Differences
Mean
Std.
Deviation
Absolute
Positive
Negative
Minggu_A Minggu_ Minggu_ Minggu_ Minggu_
wal
1
2
3
4
15
15
15
15
15
42,6000 39,4000 36,0000 32,5333 29,4667
1,91982
2,35433
4,65986
7,70776
8,64264
,217
,217
-,111
,217
,055c
,201
,133
-,201
,201
,107c
,185
,101
-,185
,185
,178c
,207
,114
-,207
,207
,084c
,167
,111
-,167
,167
,200c,d
F
Sig.
Test Statistic
Asymp. Sig. (2-tailed)
a. Test distribution is Normal.
b. Calculated from data.
c. Lilliefors Significance Correction.
d. This is a lower bound of the true significance.
ANOVA
Sum of
Squares
Minggu_A
wal
Minggu_1
Minggu_2
Minggu_3
Minggu_4
Between
Groups
Mean
Square
df
30,267
4
7,567
Within Groups
Total
Between
Groups
21,333
51,600
10
14
2,133
52,933
4
13,233
Within Groups
Total
Between
Groups
24,667
77,600
10
14
2,467
274,000
4
68,500
Within Groups
Total
Between
Groups
30,000
304,000
10
14
3,000
796,400
4
199,100
Within Groups
Total
Between
Groups
35,333
831,733
10
14
3,533
1005,733
4
251,433
Within Groups
Total
40,000
1045,733
10
14
4,000
3,547
,048
5,365
,014
22,833
,000
56,349
,000
62,858
,000
87
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Homogeneous Subsets
Minggu_Awal
Tukey HSD
a
Subset for alpha = 0.05
Kelompok
N
1
2
Formula 1
3
41,0000
Formula 2
3
41,6667
41,6667
Formula 0
3
42,0000
42,0000
Formula 3
3
43,3333
43,3333
Formula 4
3
45,0000
Sig.
,350
,107
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_1
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
Formula 4
3
36,3333
Formula 3
3
38,6667
38,6667
Formula 2
3
39,6667
39,6667
Formula 1
3
40,3333
40,3333
Formula 0
3
42,0000
Sig.
,065
,144
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_2
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Formula 4
3 28,3333
Formula 3
3
36,0000
Formula 2
3
36,3333
Formula 1
3
38,0000 38,0000
Formula 0
3
41,3333
Sig.
1,000
,633
,204
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
88
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Minggu_3
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Formula 4
3
19,3333
Formula 3
3
30,6667
Formula 2
3
35,6667
35,6667
Formula 1
3
36,6667
Formula 0
3
40,3333
Sig.
1,000
,053
,074
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_4
Tukey HSDa
Subset for alpha = 0.05
Kelompo
k
N
1
2
3
4
Formula
3 15,0000
4
Formula
3
27,3333
3
Formula
3
31,3333 31,3333
2
Formula
3
34,6667 34,6667
1
Formula
3
39,0000
0
Sig.
1,000
,179
,314
,133
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
89
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Keriput (Wrinkle)
One-Sample Kolmogorov-Smirnov Test
Minggu_A
wal
N
Minggu_ Minggu_ MInggu_ Minggu_
1
2
3
4
15
15
15
15
15
28,4000
26,4667
24,8000
22,7333
20,6000
,98561
1,68466
2,59670
3,95450
5,34255
Test Statistic
,262
,205
-,262
,262
,176
,176
-,124
,176
,221
,221
-,112
,221
,174
,174
-,131
,174
,127
,127
-,117
,127
Asymp. Sig. (2-tailed)
,007c
,200c,d
,047c
,200c,d
,200c,d
F
Sig.
Normal Parametersa,b
Mean
Std.
Deviation
Absolute
Most Extreme
Differences
Positive
Negative
a. Test distribution is Normal.
b. Calculated from data.
c. Lilliefors Significance Correction.
d. This is a lower bound of the true significance.
ANOVA
Sum of
Squares
Minggu_Aw Between Groups
al
Minggu_1
Minggu_2
MInggu_3
Mean Square
3,600
4
,900
Within Groups
10,000
10
1,000
Total
13,600
14
Between Groups
28,400
4
7,100
Within Groups
11,333
10
1,133
Total
39,733
14
Between Groups
84,400
4
21,100
Within Groups
10,000
10
1,000
Total
94,400
14
204,933
4
51,233
14,000
10
1,400
Total
218,933
14
Between Groups
388,267
4
97,067
11,333
10
1,133
399,600
14
Between Groups
Within Groups
Minggu_4
df
Within Groups
Total
,900
,499
6,265
,009
21,100
,000
36,595
,000
85,647
,000
90
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Homogeneous Subsets
Minggu_Awal
a
Tukey HSD
Subset for alpha =
0.05
Kelompok
N
1
Formula 1
3
27,6667
Formula 3
3
28,0000
Formula 2
3
28,6667
Formula 4
3
28,6667
Formula 0
3
29,0000
Sig.
,511
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_1
a
Tukey HSD
Kelompok
Formula 4
Formula 3
Formula 2
Formula 1
Formula 0
Sig.
Subset for alpha = 0.05
1
2
N
3
3
3
3
3
25,0000
25,6667
26,0000
26,6667
,368
26,6667
29,0000
,127
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
91
Universitas Sumatera Utara
Lampiran 13. (Lanjutan)
Minggu_2
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Formula 4
3
22,0000
Formula 3
3
23,3333
23,3333
Formula 2
3
24,3333
24,3333
Formula 1
3
Formula 0
3
25,3333
29,0000
Sig.
,098
,179
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MInggu_3
Tukey HSDa
Kelompok
Formula 4
N
Subset for alpha = 0.05
2
1
3
3
17,6667
Formula 3
3
21,3333
Formula 2
3
22,0000
Formula 1
3
23,6667
Formula 0
3
29,0000
Sig.
1,000
,188
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_4
Tukey HSDa
Kelompok
Formula 4
N
1
3
Subset for alpha = 0.05
2
3
14,0000
Formula 3
3
17,3333
Formula 2
3
20,0000
Formula 1
3
Formula 0
3
Sig.
4
20,0000
22,6667
29,0000
1,000
,071
,071
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
92
Universitas Sumatera Utara