PROFIL LIPID DAN INDEKS ATEROGENIK TIKUS
Seminar Nasional Biodiversitas VI, Surabaya 3 September 2016
PROFIL LIPID DAN INDEKS ATEROGENIK TIKUS PUTIH (Rattus norvegicus
Berkenhout, 1769) HIPERLIPIDEMIA DENGAN ASUPAN PELET NASI DAN BEKATUL
BERAS HITAM (Oryza sativa L.) “CEMPO IRENG”
Noor Nailis Sa’adah1, Rarastoeti Pratiwi2*
1
Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Teknologi Sepuluh
Nopember, Surabaya, Indonesia.
2
Jurusan Biologi, Fakultas Biologi, Universitas Gadjah Mada, Yogyakarta, Indonesia.
*Corresponding author : Telp. (0274) 902350, 580839, Fax. (0274) 580589,
Email: rarastp@yahoo.com*
___________________________________________________________________________________________________
ABSTRACT
Diet with high lipids cause hyperlipidemia. Hyperlipidemia stimulate leukocytes to produce ROS (Reactive
Oxygen Species) thereby increasing LDL oxidation. Macrophages phagocyt oxidized-LDL and form plaque.
Black rice has high fiber and anthocyanin, which act as antioxidants and can improve the hyperlipidemia
condition. This study was aimed to determine lipid profile and atherogenic index of hyperlipidemic Wistar rats
(Rattus norvegicus Berkenhout, 1769) which consumed rice and bran of black rice (Oryza sativa L.) “Cempo
Ireng”. The research was done with pre and post test randomized control group design. Rats were given a
mixture of lard and duck egg yolk (1:1), and pure cholesterol (2% of total volume) orally as much as 1% of body
weight (BW) for 30 days. After hyperlipidemia achieved, rats were divided into 4 group: normal rats,
hyperlipidemic rats, hyperlipidemic rats were fed a diet of 30% black rice pellet, and hyperlipidemic rats were fed
a diet of 10% black rice bran pellet. Blood samples were collected when rats in hyperlipidemia conditions and
after treatment with black rice for 30 days. The data of total cholesterol, HDL-Cholesterol level, and atherogenic
index were analyzed using ANOVA followed by Tukey test at 5% significance level. The result showed that
feeding pellet of rice and bran of black rice "Cempo Ireng" in hyperlipidemic rats decreased the total cholesterol
levels and increased of HDL-cholesterol levels significantly (p
PROFIL LIPID DAN INDEKS ATEROGENIK TIKUS PUTIH (Rattus norvegicus
Berkenhout, 1769) HIPERLIPIDEMIA DENGAN ASUPAN PELET NASI DAN BEKATUL
BERAS HITAM (Oryza sativa L.) “CEMPO IRENG”
Noor Nailis Sa’adah1, Rarastoeti Pratiwi2*
1
Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Teknologi Sepuluh
Nopember, Surabaya, Indonesia.
2
Jurusan Biologi, Fakultas Biologi, Universitas Gadjah Mada, Yogyakarta, Indonesia.
*Corresponding author : Telp. (0274) 902350, 580839, Fax. (0274) 580589,
Email: rarastp@yahoo.com*
___________________________________________________________________________________________________
ABSTRACT
Diet with high lipids cause hyperlipidemia. Hyperlipidemia stimulate leukocytes to produce ROS (Reactive
Oxygen Species) thereby increasing LDL oxidation. Macrophages phagocyt oxidized-LDL and form plaque.
Black rice has high fiber and anthocyanin, which act as antioxidants and can improve the hyperlipidemia
condition. This study was aimed to determine lipid profile and atherogenic index of hyperlipidemic Wistar rats
(Rattus norvegicus Berkenhout, 1769) which consumed rice and bran of black rice (Oryza sativa L.) “Cempo
Ireng”. The research was done with pre and post test randomized control group design. Rats were given a
mixture of lard and duck egg yolk (1:1), and pure cholesterol (2% of total volume) orally as much as 1% of body
weight (BW) for 30 days. After hyperlipidemia achieved, rats were divided into 4 group: normal rats,
hyperlipidemic rats, hyperlipidemic rats were fed a diet of 30% black rice pellet, and hyperlipidemic rats were fed
a diet of 10% black rice bran pellet. Blood samples were collected when rats in hyperlipidemia conditions and
after treatment with black rice for 30 days. The data of total cholesterol, HDL-Cholesterol level, and atherogenic
index were analyzed using ANOVA followed by Tukey test at 5% significance level. The result showed that
feeding pellet of rice and bran of black rice "Cempo Ireng" in hyperlipidemic rats decreased the total cholesterol
levels and increased of HDL-cholesterol levels significantly (p