Uji Efek Antiinfsi Jus Buah Belimbing Manis (Averrhoa carambola L.) Terhadap Tikus Jantan
Lampiran 1. Hasil Identifikasi Tumbuhan
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Lampiran 2.Komite Etik Penelitian Hewan
46
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Lampiran 3.Gambar Tumbuhan Buah Belimbing Manis (Averrhoa carambola
L.)
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Lampiran 4.Gambar Buah Segar dan Simplisia Belimbing Manis
Gambar.Buah segar belimbing manis
Gambar. Simplisia buah belimbing manis
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Lampiran 5.Gambar Mikroskopik Serbuk Simplisia
1
2
3
4
5
6
7
Gambar.Mikroskopik serbuk simplisia perbesaran 10 x 40
Keterangan :
1. Berkas pengangkut
2. Parenkim
3. Endosperm berisi butir pati
4. Kristal kalsium oksalat bentuk druse
5. Epidermis
6. Serabut
7. Sel batu
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Lampiran 6.Perhitungan Hasil Pemeriksaan Karakterisasi Simplisia
1. Perhitungan penetapan kadar air
volume air (ml )
% Kadar Air = berat
sampel (g)
× 100%
a. Berat sampel
= 5,089 g
Volume air
= 0,4 ml
% Kadar air
= 5,089 g × 100% = 7,86%
0,4 ml
b. Berat sampel
= 5,004 g
Volume air
= 0,4 ml
% Kadar air
= 5,004 g × 100% = 7,99%
0,4 ml
c. Berat sampel
= 5,116 g
Volume air
= 0,4 ml
% Kadar air
= 5,116 g × 100% = 7,81%
0,4 ml
% Rata-rata kadar air = 7,86% + 7,99% + 7,81% = 7,88%
3
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Lampiran 6.Lanjutan
2. Perhitungan penetapan kadar sari larut dalam air
berat sari (g)
×
% Kadar Sari Larut dalam Air = berat
sampel (g)
a. Berat sampel
= 5,100 g
Berat sari
= 0,237 g
% Kadar sari larut dalam air
= 5,100 ×
0,237
100
20
b. Berat sampel
= 5,026 g
Berat sari
= 0,203 g
% Kadar sari larut dalam air
= 5,026 ×
0,203
100
20
c. Berat sampel
= 5,020 g
Berat sari
= 0,218 g
% Kadar sari larut dalam air
= 5,020 ×
0,218
100
100
20
20
× 100%
× 100% = 23,23%
× 100% = 20,19%
× 100% = 21,71%
% Rata-rata kadar sari larut dalam air = 23,23% + 20,19% + 21,71% = 21,71%
3
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Lampiran 6. Lanjutan
3. Perhitungan penetapan kadar sari larut dalam etanol
berat sari (g)
% Kadar Sari Larut dalam Etanol = berat
sampel (g)
a. Berat sampel
= 5,101 g
Berat sari
= 0,131 g
% Kadar sari larut dalam etanol
= 5,101 ×
b. Berat sampel
0,131
100
20
×
100
20
× 100%
× 100% = 12,84%
= 5,007 g
Berat sari
= 0,1 g
% Kadar sari larut dalam etanol
= 5,007 ×
0,1
100
20
c. Berat sampel
= 5,057 g
Berat sari
= 0,161 g
% Kadar sari larut dalam etanol
= 5,057 ×
0,161
100
20
× 100% = 9,98%
× 100% = 15,91%
% Rata-rata kadar sari larut dalam etanol = 12,84% + 9,98% + 15,91%
3
= 12,91%
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Lampiran 6. Lanjutan
4. Perhitungan penetapan kadar abu total
berat abu (g)
% Kadar Abu Total = berat
sampel (g)
a. Berat sampel
= 2,0003 g
Berat Abu
= 0,0719 g
× 100%
0,0719
% Kadar abu total = 2,0003 × 100% = 3,59%
b. Berat sampel
= 2,0003 g
Berat Abu
= 0,0737 g
% Kadar abu total =
0,0737
2,0003
× 100% = 3,68%
c. Berat sampel
= 2,0003 g
Berat Abu
= 0,0760 g
% Kadar abu total =
0,0760
2,0003
× 100% = 3,79%
% Rata-rata kadar abu total = 3,59% + 3,68% + 3,79% = 3,68%
3
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Lampiran 6. Lanjutan
5. Perhitungan penetapan kadar abu tidak larut dalam asam
berat abu (g)
% Kadar Abu Tidak Larut dalam Asam = berat
a. Berat sampel
= 2,0003 g
Berat Abu
= 0,0030 g
sampel (g)
× 100%
0,0030
% Kadar abu tidak larut dalam asam = 2,0003 × 100% = 0,15%
b. Berat sampel
= 2,0003 g
Berat Abu
= 0,0025 g
% Kadar abu tidak larut dalam asam =
c. Berat sampel
= 2,0003 g
Berat Abu
= 0,0025 g
% Kadar abu tidak larut dalam asam =
0,0025
2,0003
0,0025
2,0003
× 100% = 0,12%
× 100% = 0,13%
% Rata-rata kadar abu tidak larut dalam asam = 0,15% + 0,12% + 0,13%
3
= 0,13%
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Lampiran 7.Bagan Kerja Penelitian
Buah Belimbing
Manis
dicuci bersih, dipotong
dagingnya sesuai ukuran
pembuatan simplisia
ditimbang sebagai berat basah
dikeringkan di lemari pengering
pada suhu 400C
Simplisia
dihaluskan dan ditimbang beratnya
Serbuk Simplisia
Karakterisasi Simplisia
Skrining Fitokimia
Pemeriksaan makroskopik
Pemeriksaan Alkaloida
Pemeriksaan mikroskopik
Pemeriksaan Glikosida
Penetapan kadar air
Pemeriksaan
Steroida/Triterpenoida
Penetapan kadar sari yang
larut dalam air
Pemeriksaan Flavonoid
Penetapan kadar sari yang
larut dalam etanol
Pemeriksaan Tanin
Pemeriksaan Saponin
Penetapan kadar abu total
Penetapan kadar abu yang
tidak larut dalam asam
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Lampiran 7.Lanjutan
Bagan Pembuatan Jus Buah Belimbing Manis
Buah Belimbing
Manis
dicuci bersih, dipotong bagian
ujung buahnya dan
ditimbang beratnya 8kg
dihaluskan menggunakan juicer
tanpa ditambah air
Jus buah
dibekukan dalam freezer
dikeringkan dengan
menggunakan freeze dryer
ditimbang beratnya = 25 g
Jus buah kering
dilakukan uji efek antiinflamasi jus
buah belimbing manis
Hasil Uji
56
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Lampiran 8.Gambar Hewan Percobaan
Telapak kaki tikus sebelum diinduksi karagenan
Telapak kaki tikus setelah diinduksi karagenan
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Lampiran 9.Contoh Perhitungan Dosis
Kontrol positif (natrium diklofenak)
Tiap tablet natrium diklofenak mengandung 50 mg natrium diklofenak
Dosis pemakaian untuk manusia 25-50 mg
Konversi dari manusia (70 kg) untuk tikus 200 g = 0,018
Pemberian larutan natrium diklofenak
Konversi dosis untuk tikus = 50 mg x 0,018 = 0,90 mg
Maka dosis natrium diklofenak yang digunakan 0,90 mg untuk tikus 200 g,
sehingga dosis dalam mg/kg bb adalah :
o,90 mg
200 g
x=
0,90 mg
200 g
x
= 1 kg
x 1000 g
x = 4,5 mg/kg bb
Bahan uji (jus buah belimbing manis)
Volume pemberian 1% dari berat badan tikus
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Lampiran 10.Contoh Perhitungan Persen Radang Dan Persen Inhibisi Radang
1. Persen Radang
Persen radang =
Vt - Vo
Vo
x 100%
Dimana :Vt = Volume udem kaki pada waktu t
Vo = Volume awal kaki tikus
Misalnya : Jus buah belimbing manis dosis 125 mg/kg BB pada menit ke-30
Diketahui : Vt =4,99
Vo = 3,41
Persen Radang =
4,99 - 3,41
3,41
x 100% =46,33%
2. Persen Inhibisi Radang
Persen inhibisi radang =
a-b
a
x 100%
Dimana : a = Persen radang rata-rata kelompok kontrol
b = Persen radang rata-rata kelompok perlakuan yang mendapat bahan
uji atau obat pembanding
Misalnya : Jus buah belimbing manis dosis 250 mg/kg BB pada menit ke-30
Diketahui : a = 51,25%
b = 33,25%
Persen Radang =
51,25 - 33,25
51,25
x 100% =35,12%
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Lampiran 11.Tabel Konversi
Tabel Perbandingan Luas Permukaan Tubuh Hewan Percobaan Untuk Konversi
Dosis (Suhardjo, 1995).
Hewan
dan BB
rata-rata
Mencit
20 g
Tikus
200 g
Marmut
400 g
Kelinci
1,5 kg
Kera
2 kg
Anjing
12 kg
Manusia
70 kg
Mencit
20 g
1,0
7,0
12,25
27,8
64,1
124,2
387,9
Tikus
200 g
0,14
1,0
1,74
3,9
9,2
17,8
56,0
Marmut
400 g
0,08
0,57
1,0
2,25
5,2
10,2
31,5
Kelinci
1,5 kg
0,04
0,25
0,44
1,0
2,4
4,5
14,2
Kera
4 kg
0,016
0,11
0,19
0,42
1,0
1,9
6,1
Anjing
12 kg
0,008
0,06
0,10
0,22
0,52
1,0
3,1
Manusia
70 kg
0,026
0,018
0,031
0,07
0,16
0,32
10
60
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Lampiran 12.Hasil Analisis Deskriptif Persen Radang
Descriptives
95% Confidence
Interval for Mean
Std.
Std.
Lower
Upper
Mini-
Maxi-
N
Mean
Deviation
Error
Bound
Bound
mum
mum
Menit
Na-CMC 0,5%
5
51.2580
1.83359
.82001
48.9813
53.5347
48.27
52.85
Ke-30
JBBM 125 mg
5
48.4780
2.00596
.89709
45.9873
50.9687
46.33
51.65
JBBM 250 mg
5
33.2520
.50732
.22688
32.6221
33.8819
32.70
33.75
JBBM 500 mg
5
28.0720
.81656
.36518
27.0581
29.0859
26.67
28.66
Na.Diklofenak
5
25.5880
.44941
.20098
25.0300
26.1460
25.00
26.22
Total
25
37.3296
10.85215
2.17043 32.8501
41.8091
25.00
52.85
Menit
Na-CMC 0,5%
5
64.7220
2.83892
1.26960 61.1970
68.2470
60.40
67.09
Ke-60
JBBM 125 mg
5
52.8620
3.36006
1.50266 48.6899
57.0341
47.51
56.76
JBBM 250 mg
5
38.9720
2.89190
1.29330 35.3812
42.5628
35.85
42.14
JBBM 500 mg
5
31.8820
.80310
.35916
30.8848
32.8792
31.10
32.83
Na-Diklofenak
5
28.4920
1.76480
.78924
26.3007
30.6833
26.57
30.79
25
43.3860
14.02270
2.80454 37.5977
49.1743
26.57
67.09
4,5mg
4,5mg
Total
Menit
Na-CMC 0,5%
5
73.3080
1.78346
.79759
71.0935
75.5225
70.52
74.93
Ke-90
JBBM 125 mg
5
57.8220
3.51432
1.57165 53.4584
62.1856
51.91
61.26
JBBM 250 mg
5
44.7580
3.28387
1.46859 40.6805
48.8355
41.19
47.80
JBBM 500 mg
5
35.3320
.67180
.30044
34.4978
36.1662
34.76
36.17
Na-
5
31.5600
2.00340
.89595
29.0725
34.0475
29.25
33.54
Total
25
48.5560
15.82000
3.16400 42.0258
55.0862
29.25
74.93
Menit
Na-CMC 0,5%
5
78.2140
3.94671
1.76502 73.3135
83.1145
73.99
84.49
Ke-120
JBBM 125 mg
5
68.8820
2.38184
1.06519 65.9246
71.8394
64.81
70.87
JBBM 250 mg
5
49.6200
3.12390
1.39705 45.7412
53.4988
45.94
52.83
JBBM 500 mg
5
41.8020
.88180
.39435
40.7071
42.8969
40.85
42.86
Na-
5
38.2280
.62564
.27979
37.4512
39.0048
37.35
39.10
Total
25
55.3492
16.08029
3.21606 48.7116
61.9868
37.35
84.49
Menit
Na-CMC 0,5%
5
84.2880
5.39696
2.41360 77.5868
90.9892
78.61
93.04
Ke-150
JBBM 125 mg
5
77.3360
3.43283
1.53521 73.0736
81.5984
72.14
81.48
JBBM 250 mg
5
51.6720
3.36571
1.50519 47.4929
55.8511
48.11
55.03
Diklofenak4,5m
g
Diklofenak4,5m
g
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JBBM 500 mg
5
44.8300
.53362
.23864
44.1674
45.4926
44.18
45.59
Na-
5
41.3040
.45346
.20280
40.7409
41.8671
40.90
41.99
Total
25
59.8860
18.15206
3.63041 52.3932
67.3788
40.90
93.04
Menit
Na-CMC 0,5%
5
86.2600
4.59834
2.05644 80.5504
91.9696
81.79
93.99
Ke-180
JBBM 125 mg
5
78.1440
3.02544
1.35302 74.3874
81.9006
73.02
81.08
JBBM 250 mg
5
54.2380
1.96717
.87975
51.7954
56.6806
52.50
56.92
JBBM 500 mg
5
46.4640
1.06453
.47607
45.1422
47.7858
45.76
48.33
Na-
5
44.2540
.58505
.26164
43.5276
44.9804
43.58
45.12
Total
25
61.8720
17.64314
3.52863 54.5893
69.1547
43.58
93.99
Menit
Na-CMC 0,5%
5
88.6120
5.25064
2.34816 82.0925
95.1315
83.24
97.15
Ke-210
JBBM 125 mg
5
81.1980
3.47513
1.55412 76.8831
85.5129
75.66
85.29
JBBM 250 mg
5
56.1740
1.14448
.51183
54.7529
57.5951
54.43
57.55
JBBM 500 mg
5
43.3760
1.00510
.44950
42.1280
44.6240
42.30
44.98
Na-
5
37.9280
.90190
.40334
36.8081
39.0479
36.45
38.81
Total
25
61.4576
20.76541
4.15308 52.8861
70.0291
36.45
97.15
Menit
Na-CMC 0,5%
5
89.2540
4.90071
2.19166 83.1690
95.3390
84.68
97.47
Ke-240
JBBM 125 mg
5
77.8440
3.60568
1.61251 73.3670
82.3210
73.02
83.18
JBBM 250 mg
5
53.8080
1.43837
.64326
52.0220
55.5940
51.99
55.66
JBBM 500 mg
5
40.8960
1.20295
.53797
39.4023
42.3897
39.94
42.30
Na-
5
34.4960
1.27365
.56959
32.9146
36.0774
32.83
36.25
Diklofenak4,5m
g
Diklofenak4,5m
g
Diklofenak4,5m
g
Diklofenak4,5m
g
Total
25
59.2596
21.69364
4.33873 50.3049
68.2143
32.83
97.47
Menit
Na-CMC 0,5%
5
86.9480
4.29621
1.92132 81.6136
92.2824
82.66
93.99
Ke-270
JBBM 125 mg
5
73.6680
4.08268
1.82583 68.5987
78.7373
68.04
79.58
JBBM 250 mg
5
50.5300
3.91581
1.75120 45.6679
55.3921
44.04
54.09
JBBM 500 mg
5
36.0000
1.09909
.49153
34.6353
37.3647
34.63
37.27
Na-
5
32.2740
.52070
.23286
31.6275
32.9205
31.63
32.93
Total
25
55.8840
21.91016
4.38203 46.8399
64.9281
31.63
93.99
Menit
Na-CMC 0,5%
5
84.1000
4.89054
2.18711 78.0276
90.1724
80.06
92.41
Ke-300
JBBM 125 mg
5
71.0360
3.11831
1.39455 67.1641
74.9079
66.57
75.38
JBBM 250 mg
5
47.8480
4.19371
1.87549 42.6408
53.0552
40.67
51.08
JBBM 500 mg
5
33.3940
.75198
.33630
34.3277
32.73
34.65
Diklofenak4,5m
g
32.4603
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Na-
5
29.4420
.27707
Total
25
53.1640
Menit
Na-CMC 0,5%
5
Ke-330
JBBM 125 mg
.12391
29.0980
29.7860
29.22
29.88
21.88886
4.37777 44.1287
62.1993
29.22
92.41
80.3680
5.15734
2.30643 73.9643
86.7717
76.30
89.24
5
68.6420
3.61850
1.61824 64.1490
73.1350
63.64
73.87
JBBM 250 mg
5
45.5460
4.15224
1.85694 40.3903
50.7017
38.53
48.92
JBBM 500 mg
5
29.1620
.62367
.27892
28.3876
29.9364
28.06
29.57
Na-
5
26.4960
.70808
.31666
25.6168
27.3752
25.67
27.44
Total
25
50.0428
21.99663
4.39933 40.9630
59.1226
25.67
89.24
Menit
Na-CMC 0,5%
5
76.5800
5.67728
2.53896 69.5307
83.6293
72.25
86.39
Ke-360
JBBM 125 mg
5
64.8160
3.38124
1.51214 60.6176
69.0144
60.12
69.67
JBBM 250 mg
5
43.8020
4.38866
1.96267 38.3528
49.2512
36.70
47.99
JBBM 500 mg
5
27.2280
.54145
.24214
26.5557
27.9003
26.27
27.58
Na-
5
24.6840
1.19169
.53294
23.2043
26.1637
22.89
25.98
25
47.4220
21.12645
4.22529 38.7014
56.1426
22.89
86.39
Diklofenak4,5m
g
Diklofenak4,5m
g
Diklofenak4,5m
g
Total
63
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Lampiran 13.Hasil Uji Analisis Variansi (ANOVA) One Way
ANOVA
Sum of
Squares
2792.411
df
4
34.048
20
Total
2826.460
24
Menit
Between
4593.381
4
1148.345
Ke-60
Groups
Within Groups
125.888
20
6.294
Total
4719.269
24
Menit
Between
5883.416
4
1470.854
Ke-90
Groups
Within Groups
123.119
20
6.156
Total
6006.536
24
Menit
Between
6077.108
4
1519.277
Ke-120
Groups
Within Groups
128.710
20
6.435
Total
6205.818
24
Menit
Between
7697.019
4
1924.255
Ke-150
Groups
210.920
20
10.546
Menit
Between
Ke-30
Groups
Within Groups
Within Groups
Mean Square
F
698.103
410.067
Sig.
.000
1.702
182.439
.000
238.931
.000
236.078
.000
182.463
.000
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Total
7907.939
24
Menit
Between
7328.154
4
1832.039
Ke-180
Groups
Within Groups
142.573
20
7.129
Total
7470.727
24
Menit
Between
10177.738
4
2544.434
Ke-210
Groups
171.117
20
8.556
Total
10348.855
24
Menit
Between
11126.108
4
2781.527
Ke-240
Groups
168.624
20
8.431
Total
11294.732
24
Menit
Between
11313.568
4
2828.392
Ke-270
Groups
207.753
20
10.388
Total
11521.322
24
Menit
Between
11291.453
4
2822.863
Ke-300
Groups
207.483
20
10.374
Total
11498.935
24
Menit
Between
11381.144
4
2845.286
Ke-330
Groups
231.293
20
11.565
Total
11612.437
24
Menit
Between
10453.294
4
2613.324
Ke-360
Groups
258.552
20
12.928
10711.846
24
Within Groups
Within Groups
Within Groups
Within Groups
Within Groups
Within Groups
Total
256.996
.000
297.391
.000
329.908
.000
272.283
.000
272.106
.000
246.033
.000
202.151
.000
65
Universitas Sumatera Utara
Lampiran 14.Hasil uji Tukey
Menitke-30
a
Tukey HSD
Kelompok
Na-Diklofenak 4,5 mg
N
1
5 25.5880
JBBM 500 mg
5
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
Subset for alpha = 0.05
2
3
4
5
28.0720
33.2520
48.4780
51.2580
1.000
1.000
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Menit ke-60
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
28.4920
JBBM 500 mg
5
31.8820
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
38.9720
52.8620
64.7220
.244
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
66
Universitas Sumatera Utara
Lampiran 14. Lanjutan
Menit ke-90
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
31.5600
JBBM 500 mg
5
35.3320
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
44.7580
57.8220
73.3080
.155
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Menit ke-120
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
38.2280
JBBM 500 mg
5
41.8020
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
49.6200
68.8820
78.2140
.210
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
67
Universitas Sumatera Utara
Lampiran 14. Lanjutan
Menit ke-150
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
41.3040
JBBM 500 mg
5
44.8300
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
51.6720
77.3360
84.2880
.447
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Menitke-180
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak4,5 mg
5
44.2540
JBBM 500 mg
5
46.4640
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
54.2380
78.1440
86.2600
.689
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
68
Universitas Sumatera Utara
Lampiran 14. Lanjutan
Menitke-210
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
37.9280
JBBM 500 mg
5
43.3760
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
56.1740
81.1980
88.6120
.055
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Menit ke-240
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
34.4960
JBBM 500 mg/kg BB
5
38.0560
JBBM 250 mg/kg BB
5
JBBM 125 mg/kg BB
5
Na-CMC 0,5%
5
Sig.
2
3
4
53.8080
77.8440
89.2540
.329
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
69
Universitas Sumatera Utara
Lampiran 14. Lanjutan
Menitke-270
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
32.2740
JBBM 500 mg
5
36.0000
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
50.5300
73.6680
86.9480
.386
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Menit ke-300
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
29.4420
JBBM 500 mg
5
33.3940
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
47.8480
71.0360
84.1000
.330
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
70
Universitas Sumatera Utara
Lampiran 14. Lanjutan
Menitke-330
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak4,5 mg
5
26.4960
JBBM 500 mg
5
29.1620
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
45.5460
68.6420
80.3680
.729
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Menit ke-360
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
24.6840
JBBM 500 mg
5
27.2280
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
43.8020
64.8160
76.5800
.795
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
71
Universitas Sumatera Utara
45
Universitas Sumatera Utara
Lampiran 2.Komite Etik Penelitian Hewan
46
Universitas Sumatera Utara
Lampiran 3.Gambar Tumbuhan Buah Belimbing Manis (Averrhoa carambola
L.)
47
Universitas Sumatera Utara
Lampiran 4.Gambar Buah Segar dan Simplisia Belimbing Manis
Gambar.Buah segar belimbing manis
Gambar. Simplisia buah belimbing manis
48
Universitas Sumatera Utara
Lampiran 5.Gambar Mikroskopik Serbuk Simplisia
1
2
3
4
5
6
7
Gambar.Mikroskopik serbuk simplisia perbesaran 10 x 40
Keterangan :
1. Berkas pengangkut
2. Parenkim
3. Endosperm berisi butir pati
4. Kristal kalsium oksalat bentuk druse
5. Epidermis
6. Serabut
7. Sel batu
49
Universitas Sumatera Utara
Lampiran 6.Perhitungan Hasil Pemeriksaan Karakterisasi Simplisia
1. Perhitungan penetapan kadar air
volume air (ml )
% Kadar Air = berat
sampel (g)
× 100%
a. Berat sampel
= 5,089 g
Volume air
= 0,4 ml
% Kadar air
= 5,089 g × 100% = 7,86%
0,4 ml
b. Berat sampel
= 5,004 g
Volume air
= 0,4 ml
% Kadar air
= 5,004 g × 100% = 7,99%
0,4 ml
c. Berat sampel
= 5,116 g
Volume air
= 0,4 ml
% Kadar air
= 5,116 g × 100% = 7,81%
0,4 ml
% Rata-rata kadar air = 7,86% + 7,99% + 7,81% = 7,88%
3
50
Universitas Sumatera Utara
Lampiran 6.Lanjutan
2. Perhitungan penetapan kadar sari larut dalam air
berat sari (g)
×
% Kadar Sari Larut dalam Air = berat
sampel (g)
a. Berat sampel
= 5,100 g
Berat sari
= 0,237 g
% Kadar sari larut dalam air
= 5,100 ×
0,237
100
20
b. Berat sampel
= 5,026 g
Berat sari
= 0,203 g
% Kadar sari larut dalam air
= 5,026 ×
0,203
100
20
c. Berat sampel
= 5,020 g
Berat sari
= 0,218 g
% Kadar sari larut dalam air
= 5,020 ×
0,218
100
100
20
20
× 100%
× 100% = 23,23%
× 100% = 20,19%
× 100% = 21,71%
% Rata-rata kadar sari larut dalam air = 23,23% + 20,19% + 21,71% = 21,71%
3
51
Universitas Sumatera Utara
Lampiran 6. Lanjutan
3. Perhitungan penetapan kadar sari larut dalam etanol
berat sari (g)
% Kadar Sari Larut dalam Etanol = berat
sampel (g)
a. Berat sampel
= 5,101 g
Berat sari
= 0,131 g
% Kadar sari larut dalam etanol
= 5,101 ×
b. Berat sampel
0,131
100
20
×
100
20
× 100%
× 100% = 12,84%
= 5,007 g
Berat sari
= 0,1 g
% Kadar sari larut dalam etanol
= 5,007 ×
0,1
100
20
c. Berat sampel
= 5,057 g
Berat sari
= 0,161 g
% Kadar sari larut dalam etanol
= 5,057 ×
0,161
100
20
× 100% = 9,98%
× 100% = 15,91%
% Rata-rata kadar sari larut dalam etanol = 12,84% + 9,98% + 15,91%
3
= 12,91%
52
Universitas Sumatera Utara
Lampiran 6. Lanjutan
4. Perhitungan penetapan kadar abu total
berat abu (g)
% Kadar Abu Total = berat
sampel (g)
a. Berat sampel
= 2,0003 g
Berat Abu
= 0,0719 g
× 100%
0,0719
% Kadar abu total = 2,0003 × 100% = 3,59%
b. Berat sampel
= 2,0003 g
Berat Abu
= 0,0737 g
% Kadar abu total =
0,0737
2,0003
× 100% = 3,68%
c. Berat sampel
= 2,0003 g
Berat Abu
= 0,0760 g
% Kadar abu total =
0,0760
2,0003
× 100% = 3,79%
% Rata-rata kadar abu total = 3,59% + 3,68% + 3,79% = 3,68%
3
53
Universitas Sumatera Utara
Lampiran 6. Lanjutan
5. Perhitungan penetapan kadar abu tidak larut dalam asam
berat abu (g)
% Kadar Abu Tidak Larut dalam Asam = berat
a. Berat sampel
= 2,0003 g
Berat Abu
= 0,0030 g
sampel (g)
× 100%
0,0030
% Kadar abu tidak larut dalam asam = 2,0003 × 100% = 0,15%
b. Berat sampel
= 2,0003 g
Berat Abu
= 0,0025 g
% Kadar abu tidak larut dalam asam =
c. Berat sampel
= 2,0003 g
Berat Abu
= 0,0025 g
% Kadar abu tidak larut dalam asam =
0,0025
2,0003
0,0025
2,0003
× 100% = 0,12%
× 100% = 0,13%
% Rata-rata kadar abu tidak larut dalam asam = 0,15% + 0,12% + 0,13%
3
= 0,13%
54
Universitas Sumatera Utara
Lampiran 7.Bagan Kerja Penelitian
Buah Belimbing
Manis
dicuci bersih, dipotong
dagingnya sesuai ukuran
pembuatan simplisia
ditimbang sebagai berat basah
dikeringkan di lemari pengering
pada suhu 400C
Simplisia
dihaluskan dan ditimbang beratnya
Serbuk Simplisia
Karakterisasi Simplisia
Skrining Fitokimia
Pemeriksaan makroskopik
Pemeriksaan Alkaloida
Pemeriksaan mikroskopik
Pemeriksaan Glikosida
Penetapan kadar air
Pemeriksaan
Steroida/Triterpenoida
Penetapan kadar sari yang
larut dalam air
Pemeriksaan Flavonoid
Penetapan kadar sari yang
larut dalam etanol
Pemeriksaan Tanin
Pemeriksaan Saponin
Penetapan kadar abu total
Penetapan kadar abu yang
tidak larut dalam asam
55
Universitas Sumatera Utara
Lampiran 7.Lanjutan
Bagan Pembuatan Jus Buah Belimbing Manis
Buah Belimbing
Manis
dicuci bersih, dipotong bagian
ujung buahnya dan
ditimbang beratnya 8kg
dihaluskan menggunakan juicer
tanpa ditambah air
Jus buah
dibekukan dalam freezer
dikeringkan dengan
menggunakan freeze dryer
ditimbang beratnya = 25 g
Jus buah kering
dilakukan uji efek antiinflamasi jus
buah belimbing manis
Hasil Uji
56
Universitas Sumatera Utara
Lampiran 8.Gambar Hewan Percobaan
Telapak kaki tikus sebelum diinduksi karagenan
Telapak kaki tikus setelah diinduksi karagenan
57
Universitas Sumatera Utara
Lampiran 9.Contoh Perhitungan Dosis
Kontrol positif (natrium diklofenak)
Tiap tablet natrium diklofenak mengandung 50 mg natrium diklofenak
Dosis pemakaian untuk manusia 25-50 mg
Konversi dari manusia (70 kg) untuk tikus 200 g = 0,018
Pemberian larutan natrium diklofenak
Konversi dosis untuk tikus = 50 mg x 0,018 = 0,90 mg
Maka dosis natrium diklofenak yang digunakan 0,90 mg untuk tikus 200 g,
sehingga dosis dalam mg/kg bb adalah :
o,90 mg
200 g
x=
0,90 mg
200 g
x
= 1 kg
x 1000 g
x = 4,5 mg/kg bb
Bahan uji (jus buah belimbing manis)
Volume pemberian 1% dari berat badan tikus
58
Universitas Sumatera Utara
Lampiran 10.Contoh Perhitungan Persen Radang Dan Persen Inhibisi Radang
1. Persen Radang
Persen radang =
Vt - Vo
Vo
x 100%
Dimana :Vt = Volume udem kaki pada waktu t
Vo = Volume awal kaki tikus
Misalnya : Jus buah belimbing manis dosis 125 mg/kg BB pada menit ke-30
Diketahui : Vt =4,99
Vo = 3,41
Persen Radang =
4,99 - 3,41
3,41
x 100% =46,33%
2. Persen Inhibisi Radang
Persen inhibisi radang =
a-b
a
x 100%
Dimana : a = Persen radang rata-rata kelompok kontrol
b = Persen radang rata-rata kelompok perlakuan yang mendapat bahan
uji atau obat pembanding
Misalnya : Jus buah belimbing manis dosis 250 mg/kg BB pada menit ke-30
Diketahui : a = 51,25%
b = 33,25%
Persen Radang =
51,25 - 33,25
51,25
x 100% =35,12%
59
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Lampiran 11.Tabel Konversi
Tabel Perbandingan Luas Permukaan Tubuh Hewan Percobaan Untuk Konversi
Dosis (Suhardjo, 1995).
Hewan
dan BB
rata-rata
Mencit
20 g
Tikus
200 g
Marmut
400 g
Kelinci
1,5 kg
Kera
2 kg
Anjing
12 kg
Manusia
70 kg
Mencit
20 g
1,0
7,0
12,25
27,8
64,1
124,2
387,9
Tikus
200 g
0,14
1,0
1,74
3,9
9,2
17,8
56,0
Marmut
400 g
0,08
0,57
1,0
2,25
5,2
10,2
31,5
Kelinci
1,5 kg
0,04
0,25
0,44
1,0
2,4
4,5
14,2
Kera
4 kg
0,016
0,11
0,19
0,42
1,0
1,9
6,1
Anjing
12 kg
0,008
0,06
0,10
0,22
0,52
1,0
3,1
Manusia
70 kg
0,026
0,018
0,031
0,07
0,16
0,32
10
60
Universitas Sumatera Utara
Lampiran 12.Hasil Analisis Deskriptif Persen Radang
Descriptives
95% Confidence
Interval for Mean
Std.
Std.
Lower
Upper
Mini-
Maxi-
N
Mean
Deviation
Error
Bound
Bound
mum
mum
Menit
Na-CMC 0,5%
5
51.2580
1.83359
.82001
48.9813
53.5347
48.27
52.85
Ke-30
JBBM 125 mg
5
48.4780
2.00596
.89709
45.9873
50.9687
46.33
51.65
JBBM 250 mg
5
33.2520
.50732
.22688
32.6221
33.8819
32.70
33.75
JBBM 500 mg
5
28.0720
.81656
.36518
27.0581
29.0859
26.67
28.66
Na.Diklofenak
5
25.5880
.44941
.20098
25.0300
26.1460
25.00
26.22
Total
25
37.3296
10.85215
2.17043 32.8501
41.8091
25.00
52.85
Menit
Na-CMC 0,5%
5
64.7220
2.83892
1.26960 61.1970
68.2470
60.40
67.09
Ke-60
JBBM 125 mg
5
52.8620
3.36006
1.50266 48.6899
57.0341
47.51
56.76
JBBM 250 mg
5
38.9720
2.89190
1.29330 35.3812
42.5628
35.85
42.14
JBBM 500 mg
5
31.8820
.80310
.35916
30.8848
32.8792
31.10
32.83
Na-Diklofenak
5
28.4920
1.76480
.78924
26.3007
30.6833
26.57
30.79
25
43.3860
14.02270
2.80454 37.5977
49.1743
26.57
67.09
4,5mg
4,5mg
Total
Menit
Na-CMC 0,5%
5
73.3080
1.78346
.79759
71.0935
75.5225
70.52
74.93
Ke-90
JBBM 125 mg
5
57.8220
3.51432
1.57165 53.4584
62.1856
51.91
61.26
JBBM 250 mg
5
44.7580
3.28387
1.46859 40.6805
48.8355
41.19
47.80
JBBM 500 mg
5
35.3320
.67180
.30044
34.4978
36.1662
34.76
36.17
Na-
5
31.5600
2.00340
.89595
29.0725
34.0475
29.25
33.54
Total
25
48.5560
15.82000
3.16400 42.0258
55.0862
29.25
74.93
Menit
Na-CMC 0,5%
5
78.2140
3.94671
1.76502 73.3135
83.1145
73.99
84.49
Ke-120
JBBM 125 mg
5
68.8820
2.38184
1.06519 65.9246
71.8394
64.81
70.87
JBBM 250 mg
5
49.6200
3.12390
1.39705 45.7412
53.4988
45.94
52.83
JBBM 500 mg
5
41.8020
.88180
.39435
40.7071
42.8969
40.85
42.86
Na-
5
38.2280
.62564
.27979
37.4512
39.0048
37.35
39.10
Total
25
55.3492
16.08029
3.21606 48.7116
61.9868
37.35
84.49
Menit
Na-CMC 0,5%
5
84.2880
5.39696
2.41360 77.5868
90.9892
78.61
93.04
Ke-150
JBBM 125 mg
5
77.3360
3.43283
1.53521 73.0736
81.5984
72.14
81.48
JBBM 250 mg
5
51.6720
3.36571
1.50519 47.4929
55.8511
48.11
55.03
Diklofenak4,5m
g
Diklofenak4,5m
g
61
Universitas Sumatera Utara
JBBM 500 mg
5
44.8300
.53362
.23864
44.1674
45.4926
44.18
45.59
Na-
5
41.3040
.45346
.20280
40.7409
41.8671
40.90
41.99
Total
25
59.8860
18.15206
3.63041 52.3932
67.3788
40.90
93.04
Menit
Na-CMC 0,5%
5
86.2600
4.59834
2.05644 80.5504
91.9696
81.79
93.99
Ke-180
JBBM 125 mg
5
78.1440
3.02544
1.35302 74.3874
81.9006
73.02
81.08
JBBM 250 mg
5
54.2380
1.96717
.87975
51.7954
56.6806
52.50
56.92
JBBM 500 mg
5
46.4640
1.06453
.47607
45.1422
47.7858
45.76
48.33
Na-
5
44.2540
.58505
.26164
43.5276
44.9804
43.58
45.12
Total
25
61.8720
17.64314
3.52863 54.5893
69.1547
43.58
93.99
Menit
Na-CMC 0,5%
5
88.6120
5.25064
2.34816 82.0925
95.1315
83.24
97.15
Ke-210
JBBM 125 mg
5
81.1980
3.47513
1.55412 76.8831
85.5129
75.66
85.29
JBBM 250 mg
5
56.1740
1.14448
.51183
54.7529
57.5951
54.43
57.55
JBBM 500 mg
5
43.3760
1.00510
.44950
42.1280
44.6240
42.30
44.98
Na-
5
37.9280
.90190
.40334
36.8081
39.0479
36.45
38.81
Total
25
61.4576
20.76541
4.15308 52.8861
70.0291
36.45
97.15
Menit
Na-CMC 0,5%
5
89.2540
4.90071
2.19166 83.1690
95.3390
84.68
97.47
Ke-240
JBBM 125 mg
5
77.8440
3.60568
1.61251 73.3670
82.3210
73.02
83.18
JBBM 250 mg
5
53.8080
1.43837
.64326
52.0220
55.5940
51.99
55.66
JBBM 500 mg
5
40.8960
1.20295
.53797
39.4023
42.3897
39.94
42.30
Na-
5
34.4960
1.27365
.56959
32.9146
36.0774
32.83
36.25
Diklofenak4,5m
g
Diklofenak4,5m
g
Diklofenak4,5m
g
Diklofenak4,5m
g
Total
25
59.2596
21.69364
4.33873 50.3049
68.2143
32.83
97.47
Menit
Na-CMC 0,5%
5
86.9480
4.29621
1.92132 81.6136
92.2824
82.66
93.99
Ke-270
JBBM 125 mg
5
73.6680
4.08268
1.82583 68.5987
78.7373
68.04
79.58
JBBM 250 mg
5
50.5300
3.91581
1.75120 45.6679
55.3921
44.04
54.09
JBBM 500 mg
5
36.0000
1.09909
.49153
34.6353
37.3647
34.63
37.27
Na-
5
32.2740
.52070
.23286
31.6275
32.9205
31.63
32.93
Total
25
55.8840
21.91016
4.38203 46.8399
64.9281
31.63
93.99
Menit
Na-CMC 0,5%
5
84.1000
4.89054
2.18711 78.0276
90.1724
80.06
92.41
Ke-300
JBBM 125 mg
5
71.0360
3.11831
1.39455 67.1641
74.9079
66.57
75.38
JBBM 250 mg
5
47.8480
4.19371
1.87549 42.6408
53.0552
40.67
51.08
JBBM 500 mg
5
33.3940
.75198
.33630
34.3277
32.73
34.65
Diklofenak4,5m
g
32.4603
62
Universitas Sumatera Utara
Na-
5
29.4420
.27707
Total
25
53.1640
Menit
Na-CMC 0,5%
5
Ke-330
JBBM 125 mg
.12391
29.0980
29.7860
29.22
29.88
21.88886
4.37777 44.1287
62.1993
29.22
92.41
80.3680
5.15734
2.30643 73.9643
86.7717
76.30
89.24
5
68.6420
3.61850
1.61824 64.1490
73.1350
63.64
73.87
JBBM 250 mg
5
45.5460
4.15224
1.85694 40.3903
50.7017
38.53
48.92
JBBM 500 mg
5
29.1620
.62367
.27892
28.3876
29.9364
28.06
29.57
Na-
5
26.4960
.70808
.31666
25.6168
27.3752
25.67
27.44
Total
25
50.0428
21.99663
4.39933 40.9630
59.1226
25.67
89.24
Menit
Na-CMC 0,5%
5
76.5800
5.67728
2.53896 69.5307
83.6293
72.25
86.39
Ke-360
JBBM 125 mg
5
64.8160
3.38124
1.51214 60.6176
69.0144
60.12
69.67
JBBM 250 mg
5
43.8020
4.38866
1.96267 38.3528
49.2512
36.70
47.99
JBBM 500 mg
5
27.2280
.54145
.24214
26.5557
27.9003
26.27
27.58
Na-
5
24.6840
1.19169
.53294
23.2043
26.1637
22.89
25.98
25
47.4220
21.12645
4.22529 38.7014
56.1426
22.89
86.39
Diklofenak4,5m
g
Diklofenak4,5m
g
Diklofenak4,5m
g
Total
63
Universitas Sumatera Utara
Lampiran 13.Hasil Uji Analisis Variansi (ANOVA) One Way
ANOVA
Sum of
Squares
2792.411
df
4
34.048
20
Total
2826.460
24
Menit
Between
4593.381
4
1148.345
Ke-60
Groups
Within Groups
125.888
20
6.294
Total
4719.269
24
Menit
Between
5883.416
4
1470.854
Ke-90
Groups
Within Groups
123.119
20
6.156
Total
6006.536
24
Menit
Between
6077.108
4
1519.277
Ke-120
Groups
Within Groups
128.710
20
6.435
Total
6205.818
24
Menit
Between
7697.019
4
1924.255
Ke-150
Groups
210.920
20
10.546
Menit
Between
Ke-30
Groups
Within Groups
Within Groups
Mean Square
F
698.103
410.067
Sig.
.000
1.702
182.439
.000
238.931
.000
236.078
.000
182.463
.000
64
Universitas Sumatera Utara
Total
7907.939
24
Menit
Between
7328.154
4
1832.039
Ke-180
Groups
Within Groups
142.573
20
7.129
Total
7470.727
24
Menit
Between
10177.738
4
2544.434
Ke-210
Groups
171.117
20
8.556
Total
10348.855
24
Menit
Between
11126.108
4
2781.527
Ke-240
Groups
168.624
20
8.431
Total
11294.732
24
Menit
Between
11313.568
4
2828.392
Ke-270
Groups
207.753
20
10.388
Total
11521.322
24
Menit
Between
11291.453
4
2822.863
Ke-300
Groups
207.483
20
10.374
Total
11498.935
24
Menit
Between
11381.144
4
2845.286
Ke-330
Groups
231.293
20
11.565
Total
11612.437
24
Menit
Between
10453.294
4
2613.324
Ke-360
Groups
258.552
20
12.928
10711.846
24
Within Groups
Within Groups
Within Groups
Within Groups
Within Groups
Within Groups
Total
256.996
.000
297.391
.000
329.908
.000
272.283
.000
272.106
.000
246.033
.000
202.151
.000
65
Universitas Sumatera Utara
Lampiran 14.Hasil uji Tukey
Menitke-30
a
Tukey HSD
Kelompok
Na-Diklofenak 4,5 mg
N
1
5 25.5880
JBBM 500 mg
5
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
Subset for alpha = 0.05
2
3
4
5
28.0720
33.2520
48.4780
51.2580
1.000
1.000
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Menit ke-60
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
28.4920
JBBM 500 mg
5
31.8820
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
38.9720
52.8620
64.7220
.244
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
66
Universitas Sumatera Utara
Lampiran 14. Lanjutan
Menit ke-90
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
31.5600
JBBM 500 mg
5
35.3320
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
44.7580
57.8220
73.3080
.155
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Menit ke-120
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
38.2280
JBBM 500 mg
5
41.8020
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
49.6200
68.8820
78.2140
.210
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
67
Universitas Sumatera Utara
Lampiran 14. Lanjutan
Menit ke-150
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
41.3040
JBBM 500 mg
5
44.8300
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
51.6720
77.3360
84.2880
.447
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Menitke-180
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak4,5 mg
5
44.2540
JBBM 500 mg
5
46.4640
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
54.2380
78.1440
86.2600
.689
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
68
Universitas Sumatera Utara
Lampiran 14. Lanjutan
Menitke-210
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
37.9280
JBBM 500 mg
5
43.3760
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
56.1740
81.1980
88.6120
.055
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Menit ke-240
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
34.4960
JBBM 500 mg/kg BB
5
38.0560
JBBM 250 mg/kg BB
5
JBBM 125 mg/kg BB
5
Na-CMC 0,5%
5
Sig.
2
3
4
53.8080
77.8440
89.2540
.329
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
69
Universitas Sumatera Utara
Lampiran 14. Lanjutan
Menitke-270
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
32.2740
JBBM 500 mg
5
36.0000
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
50.5300
73.6680
86.9480
.386
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Menit ke-300
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
29.4420
JBBM 500 mg
5
33.3940
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
47.8480
71.0360
84.1000
.330
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
70
Universitas Sumatera Utara
Lampiran 14. Lanjutan
Menitke-330
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak4,5 mg
5
26.4960
JBBM 500 mg
5
29.1620
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
45.5460
68.6420
80.3680
.729
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Menit ke-360
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Na-Diklofenak 4,5 mg
5
24.6840
JBBM 500 mg
5
27.2280
JBBM 250 mg
5
JBBM 125 mg
5
Na-CMC 0,5%
5
Sig.
2
3
4
43.8020
64.8160
76.5800
.795
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
71
Universitas Sumatera Utara