Pengujian Sediaan Gel Ekstrak Etanol Daun Kelapa Sawit(Elaeis guineensis Jacq.) Sebagai Obat Luka Bakar
PENGUJIAN SEDIAAN GEL EKSTRAK ETANOL DAUN KELAPA SAWIT
(Elaeis guineensis Jacq.) SEBAGAI OBAT LUKA BAKAR
Abstrak
Daun kelapa sawit (Elaeis guineensis Jacq.) mengandung alkaloid yang
memiliki kemampuan sebagai antibakteri, flavonoid yang memiliki aktivitas
astringen dan saponin yang dapat memacu pembentukan kolagen yang berperan
dalam proses penyembuhan luka. Penelitian ini bertujuan untuk menguji efektivitas
sediaan gel ekstrak etanol daun kelapa sawit terhadap penyembuhan luka bakar.
Serbuk daun kelapa sawit diekstraksi dengan pelarut etanol 80% secara
maserasi selama 5 hari, diserkai, ampasnya dicuci dengan etanol. Filtrat didiamkan
selama 2 hari kemudian dienap tuangkan. Maserat yang diperoleh diuapkan dengan
bantuan rotary evaporator (±500C) dan di freeze dryer (-40oC). Serbuk simplisia dan
ekstrak etanol daun kelapa sawit (EEDKS) dikarakterisasi dan diformulasi menjadi
sediaan gel berbasis HPMC dengan konsentrasi 2,5, 5, dan 7,5%. Selanjutnya diuji
efektivitasnya terhadap kelinci yang dibuat luka bakar (diameter 2,2 cm) dengan
pengolesan sediaan gel EEDKS satu kali sehari dimulai dari hari ke-0 sampai luka
bakar sembuh, sebagai pembanding digunakan bioplacenton, kemudian sediaan gel
diuji stabilitasnya.
Hasil karakterisasi serbuk simplisia dan EEKDS masing-masing diperoleh
kadar air (6,64% dan 2,65%), kadar sari larut air (13,49% dan 19,57%), kadar sari
larut etanol (16,98% dan 43,88%), kadar abu total (3,75% dan 2,43%) dan kadar abu
yang tidak larut asam (0,78% dan 0,24%). Hasil pemeriksaan sediaan gel EEDKS
mampu menyembuhkan luka bakar dengan konsentrasi 7,5% (18 hari), 5% (20 hari),
2,5% (21 hari). Konsentrasi yang paling efektif adalah 7,5% memberikan hasil yang
signifikan dengan bioplacenton (18 hari). Hasil uji stabilitas gel EEDKS stabil
selama 28 hari penyimpanan, hasil sediaan gel homogen, dengan nilai pH 6,6-6,9,
nilai viskositas basis gel (3500cps), gel EEDKS 2,5% (3200cps), gel EEDKS 5%
(3000cps), gel EEDKS 7,5% (2800cps).
Kata kunci : ekstrak etanol daun kelapa sawit, gel, luka bakar.
Universitas Sumatera Utara
EFFECTIVENESS TEST THE ETHANOL EXTRACT GEL OF OIL PALM
LEAF (Elaeis guineensis Jacq.) FOR BURNS HEALING
Abstract
Leaves of palm (Elaeis guineensis Jacq.) Contain alkaloids that have the
ability as an antibacterial, astringent activity of flavonoids and saponins that can
stimulate the formation of collagen, which plays a role in wound healing process and.
This study aimed to test the effectiveness of the ethanol extract gel palm leaves for
the healing of burns.
Palm’ leaf powder macerated by ethanol 80% for 5 days, filtered, the residue
has extraction by ethanol, then the filtrate leave for 2 day and poured ponder. The
maserat has evaporated by using rotary evaporator (±500C) and dried by freeze dryer
(±-40oC). Simplicia powder and ethanol extract of palm’s leaf have executed in
characteristic and then has formulated to gel preparations that have HPMC for the
basic with its concentration 2.2, 5, and 7.5%. Subsequently, gel preparations tested
its effectiveness on the backs of rabbit made burns with a diameter 2.2 cm by
measuring diameter and applying ethanol extract gel of oil palm leaf once a day
starting from 0 day until the burns healed, then gel preparations tested his stability
which include examining homogenity, pH and viscosity.
The result from characteristic simplicia powder and extract respectively have
obtained by water level (6.64% and 2.65%), water soluble extract (13,49% and
19.57%), ethanol soluble extract level (16.98% and 43.88%), total ash content
(3.75% and 2.43%), and ash level which insoluble in acid (0.78% and 0.24%). The
results of examination have significant correlation to healing the wound with each
concentration 7.5% (18 days), 5.0% (20 days), 2.5% (21 days), the most effective
concentration is 7.5% which can provide significant results with Bioplacenton as a
comparison (18 days). Results of stability test of ethanol extract of oil palm’s leaf is
stable in storage for 28 days. Homogenity examination of gel preparation have
showed homogenous, the pH value of gel preparation is 6.6-6.9, viscosity value of
basic gel is 3500 sentipoise, 3200 sentipoise for gel 2.5% , 3000 sentipoise for gel
5%, and 2800 sentipoise for gel 7.5%.
Keywords: palm leaf ethanol extract, gel, burns.
Universitas Sumatera Utara
(Elaeis guineensis Jacq.) SEBAGAI OBAT LUKA BAKAR
Abstrak
Daun kelapa sawit (Elaeis guineensis Jacq.) mengandung alkaloid yang
memiliki kemampuan sebagai antibakteri, flavonoid yang memiliki aktivitas
astringen dan saponin yang dapat memacu pembentukan kolagen yang berperan
dalam proses penyembuhan luka. Penelitian ini bertujuan untuk menguji efektivitas
sediaan gel ekstrak etanol daun kelapa sawit terhadap penyembuhan luka bakar.
Serbuk daun kelapa sawit diekstraksi dengan pelarut etanol 80% secara
maserasi selama 5 hari, diserkai, ampasnya dicuci dengan etanol. Filtrat didiamkan
selama 2 hari kemudian dienap tuangkan. Maserat yang diperoleh diuapkan dengan
bantuan rotary evaporator (±500C) dan di freeze dryer (-40oC). Serbuk simplisia dan
ekstrak etanol daun kelapa sawit (EEDKS) dikarakterisasi dan diformulasi menjadi
sediaan gel berbasis HPMC dengan konsentrasi 2,5, 5, dan 7,5%. Selanjutnya diuji
efektivitasnya terhadap kelinci yang dibuat luka bakar (diameter 2,2 cm) dengan
pengolesan sediaan gel EEDKS satu kali sehari dimulai dari hari ke-0 sampai luka
bakar sembuh, sebagai pembanding digunakan bioplacenton, kemudian sediaan gel
diuji stabilitasnya.
Hasil karakterisasi serbuk simplisia dan EEKDS masing-masing diperoleh
kadar air (6,64% dan 2,65%), kadar sari larut air (13,49% dan 19,57%), kadar sari
larut etanol (16,98% dan 43,88%), kadar abu total (3,75% dan 2,43%) dan kadar abu
yang tidak larut asam (0,78% dan 0,24%). Hasil pemeriksaan sediaan gel EEDKS
mampu menyembuhkan luka bakar dengan konsentrasi 7,5% (18 hari), 5% (20 hari),
2,5% (21 hari). Konsentrasi yang paling efektif adalah 7,5% memberikan hasil yang
signifikan dengan bioplacenton (18 hari). Hasil uji stabilitas gel EEDKS stabil
selama 28 hari penyimpanan, hasil sediaan gel homogen, dengan nilai pH 6,6-6,9,
nilai viskositas basis gel (3500cps), gel EEDKS 2,5% (3200cps), gel EEDKS 5%
(3000cps), gel EEDKS 7,5% (2800cps).
Kata kunci : ekstrak etanol daun kelapa sawit, gel, luka bakar.
Universitas Sumatera Utara
EFFECTIVENESS TEST THE ETHANOL EXTRACT GEL OF OIL PALM
LEAF (Elaeis guineensis Jacq.) FOR BURNS HEALING
Abstract
Leaves of palm (Elaeis guineensis Jacq.) Contain alkaloids that have the
ability as an antibacterial, astringent activity of flavonoids and saponins that can
stimulate the formation of collagen, which plays a role in wound healing process and.
This study aimed to test the effectiveness of the ethanol extract gel palm leaves for
the healing of burns.
Palm’ leaf powder macerated by ethanol 80% for 5 days, filtered, the residue
has extraction by ethanol, then the filtrate leave for 2 day and poured ponder. The
maserat has evaporated by using rotary evaporator (±500C) and dried by freeze dryer
(±-40oC). Simplicia powder and ethanol extract of palm’s leaf have executed in
characteristic and then has formulated to gel preparations that have HPMC for the
basic with its concentration 2.2, 5, and 7.5%. Subsequently, gel preparations tested
its effectiveness on the backs of rabbit made burns with a diameter 2.2 cm by
measuring diameter and applying ethanol extract gel of oil palm leaf once a day
starting from 0 day until the burns healed, then gel preparations tested his stability
which include examining homogenity, pH and viscosity.
The result from characteristic simplicia powder and extract respectively have
obtained by water level (6.64% and 2.65%), water soluble extract (13,49% and
19.57%), ethanol soluble extract level (16.98% and 43.88%), total ash content
(3.75% and 2.43%), and ash level which insoluble in acid (0.78% and 0.24%). The
results of examination have significant correlation to healing the wound with each
concentration 7.5% (18 days), 5.0% (20 days), 2.5% (21 days), the most effective
concentration is 7.5% which can provide significant results with Bioplacenton as a
comparison (18 days). Results of stability test of ethanol extract of oil palm’s leaf is
stable in storage for 28 days. Homogenity examination of gel preparation have
showed homogenous, the pH value of gel preparation is 6.6-6.9, viscosity value of
basic gel is 3500 sentipoise, 3200 sentipoise for gel 2.5% , 3000 sentipoise for gel
5%, and 2800 sentipoise for gel 7.5%.
Keywords: palm leaf ethanol extract, gel, burns.
Universitas Sumatera Utara