Ultrastruktur Hepar Mencit (Mus Musculus L.) Setelah Pemberian Ekstrak Air Biji Pepaya (Carica Papaya L.) Dan Testosteron Undekanoat (Tu)

  Lampiran A. Data Rataan Kerusakan Hati Berupa Nekrosis

Rataaan kerusakan hati mencit (Mus musculus L.) setelah pemberian kombinasi

testosteron undekanoat (TU) dan ekstrak air biji pepaya (Carica papaya L.)

(x±SD) Lama Pemberian (Minggu) Kontrol (%) Perlakuan (%)

  T0 13,2±6,9 17,8±9,9 T1 9,2±3,4 23,3±4,5 T2 10,4±5,4 28,1±7,4 T3

  8,8±2,7 18,1±3,5 T4 11,4±3,1 16,8±3,4 Hasil uji statistik SPSS

a. Kelompok Kontrol Tests of Normality

  Kel.KONTROL K0 K1 K2 K3 K4 NEKROSIS Statistic df Sig. Statistic df Sig.

  Kolmogorov-Smirnov

  a

  Shapiro-Wilk This is a lower bound of the true s ignificance. *.

  .244 5 .200* .894 5 .376 .192 5 .200* .923 5 .552 .192 5 .200* .923 5 .552 .198 5 .200* .947 5 .716 .223 5 .200* .968 5 .862

  Test of Homogeneity of Variance

  1.355

  4 20 .285 .667

  4 20 .622 .667 4 17.134 .623

  1.354

  4 20 .285 Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean

  NEKROSIS Levene Statistic df1 df2 Sig.

  Lilliefors Significance Correction a.

  Oneway ANOV A

  NE KROSIS .099 4 .025 .839 .517 .590 20 .030 .690

  24 Between Groups W ithin Groups Total

  Sum of Squares df Mean S quare F Sig.

  Post Hoc Tests

Multiple Comparisons

  Dependent Variable: NEKROSIS Bonferroni .13984 .10867 1.000 -.2028 .4825 .13984 .10867 1.000 -.2028 .4825 .14857 .10867 1.000 -.1941 .4913 .03220 .10867 1.000 -.3105 .3749

  K2 K3 K4 K0 K2 K3 K4 K0 K1 K3 K4 K0 K1 K2 K4 K0 K1 K2 K3 (I) Kel.KONTROL K0 K1 K2 K3 K4

  Mean Difference (I-J) Std. Error Sig. Lower Bound Upper Bound 95% Confidence Interval

• .13984 .10867 1.000 -.4825 .2028 .00000 .10867 1.000 -.3427 .3427 .00873 .10867 1.000 -.3340 .3514
• .10764 .10867 1.000 -.4503 .2350
• .13984 .10867 1.000 -.4825 .2028 .00000 .10867 1.000 -.3427 .3427 .00873 .10867 1.000 -.3340 .3514
• .10764 .10867 1.000 -.4503 .2350
• .14857 .10867 1.000 -.4913 .1941
• .00873 .10867 1.000 -.3514 .3340
• .00873 .10867 1.000 -.3514 .3340
• .11637 .10867 1.000 -.4591 .2263
• .03220 .10867 1.000 -.3749 .3105 .10764 .10867 1.000 -.2350 .4503 .10764 .10867 1.000 -.2350 .4503 .11637 .10867 1.000 -.2263 .4591 (J) Kel.KONTROL K1
• .

  b.Kelompok Perlakuan Tests of Normality .286 5 .200* .890 5 .359 .328

  4 20 .120 2.088 4 10.579 .153 2.939

  Sum of Squares df Mean S quare F Sig.

  24 Between Groups W ithin Groups Total

  .004 4 .001 2.058 .125 .009 20 .000 .013

  Oneway

ANOV A

  NEKROSIS Levene Statistic df1 df2 Sig.

  4 20 .046 Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean

  4 20 .051 2.088

  5 .084 .802 5 .084 .268 5 .200* .868 5 .257 .152 5 .200* .971 5 .882 .238 5 .200* .911 5 .473 perlakuan

  2.846

  Test of Homogeneity of Variance

  Lilliefors Significance Correction a.

  Shapiro-Wilk This is a lower bound of the true s ignificance.

  Kolmogorov-Smirnov

a

  P0 P1 P2 P3 P4 NEKROSIS Statistic df Sig. Statistic df Sig.

NE KROSIS

  Interval of the Difference t-test for Equality of Means

  (I-J) Std. Error Sig. Lower Bound Upper Bound 95% Confidence Interval

  Difference Std. Error Difference Lower Upper 95% Confidence

  Levene's Test for Equality of Variances t df Sig. (2-tailed) Mean

  

Independent Samples Test

2.137 .182 -.836 8 .427 -4.50000 5.38331 -16.91393 7.91393

  Mean

  N Mean Std. Deviation Std. Error

  5 13.3000 6.86112 3.06839 5 17.8000 9.89065 4.42323 kel_nekrosis KONTROL PERLAKUAN K0P0

  K0P0 (T-Test) Group Statistics

  Mean Difference

  (I) perlakuan P0 P1 P2 P3 P4

  (J) perlakuan P1 P2 P3 P4 P0 P2 P3 P4 P0 P1 P3 P4 P0 P1 P2 P4 P0 P1 P2 P3

• .01273 .01360 1.000 -.0556 .0302
• .01750 .01360 1.000 -.0604 .0254
• .03453 .01360 .196 -.0774 .0084
• .00600 .01360 1.000 -.0489 .0369
• .01873 .01360 1.000 -.0616 .0242
• .02350 .01360 .996 -.0664 .0194
• .01580 .01360 1.000 -.0587 .0271

  .01750 .01360 1.000 -.0254 .0604 .02350 .01360 .996 -.0194 .0664 .00477 .01360 1.000 -.0381 .0477

  .01273 .01360 1.000 -.0302 .0556 .01873 .01360 1.000 -.0242 .0616

  .00600 .01360 1.000 -.0369 .0489

  .02854 .01360 .488 -.0144 .0714 .03453 .01360 .196 -.0084 .0774 .01580 .01360 1.000 -.0271 .0587 .01104 .01360 1.000 -.0319 .0539

  Dependent Variable: NEKROSIS Bonferroni

  Post Hoc Tests Multiple Comparisons

• .02854 .01360 .488 -.0714 .0144
• .00477 .01360 1.000 -.0477 .0381
• .01104 .01360 1.000 -.0539 .0319
• .836 7.126 .430 -4.50000 5.38331 -17.18406 8.18406 Equal variances as sumed Equal variances not ass umed K0P0 F Sig.
• 5.604 7.520 .001 -14.10000 2.51595 -19.96695 -8.23305 Equal variances as sumed Equal variances not ass umed K1P1 F Sig.

  Mean

  N Mean Std. Deviation Std. Error

  N Mean Std. Deviation Std. Error

  5 8.8000 2.65989 1.18954 5 18.1000 3.52491 1.57639 kel_nekrosis KONTROL PERLAKUAN K3P3

  K3P3 (T-Test) Group Statistics

  Interval of the Difference t-test for Equality of Means

  Difference Std. Error Difference Lower Upper 95% Confidence

  Levene's Test for Equality of Variances

t df Sig. (2-tailed)

Mean

  

Independent Samples Test

1.100 .325 -5.192 8 .001 -18.90000 3.64005 -27.29398 -10.50602

  Mean

  5 9.2000 3.43875 1.53786 5 28.1000 7.37733 3.29924 kel_nekrosis KONTROL PERLAKUAN K2P2

  K1P1 (T-Test) Group Statistics

  K2P2 (T-Test) Group Statistics

  Interval of the Difference t-test for Equality of Means

  Difference Std. Error Difference Lower Upper 95% Confidence

  Levene's Test for Equality of Variances

t df Sig. (2-tailed)

Mean

  

Independent Samples Test

.696 .428 -5.604 8 .001 -14.10000 2.51595 -19.90179 -8.29821

  Mean

  N Mean Std. Deviation Std. Error

  5 9.2000 3.43875 1.53786 5 23.3000 4.45253 1.99123 kel_nekrosis KONTROL PERLAKUAN K1P1

• 5.192 5.660 .002 -18.90000 3.64005 -27.93829 -9.86171 Equal variances as sumed Equal variances not ass umed K2P2 F Sig.

  Independent Samples Test .288 .606 -4.709 8 .002 -9.30000 1.97484 -13.85399 -4.74601

  Levene's Test for Equality of Variances t df Sig. (2-tailed) Mean

  Difference Std. Error Difference Lower Upper 95% Confidence

  Interval of the Difference t-test for Equality of Means

• 4.709 7.440 .002 -9.30000 1.97484 -13.91436 -4.68564 Equal variances as sumed Equal variances not ass umed K3P3 F Sig.

  K4P4 (T-Test) Group Statistics

  5 11.4000 3.07002 1.37295 5 16.8000 3.40221 1.52151 kel_nekrosis KONTROL PERLAKUAN K4P4

  N Mean Std. Deviation Std. Error

  Mean

  

Independent Samples Test

.228 .646 -2.635 8 .030 -5.40000 2.04939 -10.12590 -.67410

  Levene's Test for Equality of Variances t df Sig. (2-tailed) Mean

  Difference Std. Error Difference Lower Upper 95% Confidence

  Interval of the Difference t-test for Equality of Means

• 2.635 7.917 .030 -5.40000 2.04939 -10.13454 -.66546 Equal variances as sumed Equal variances not ass umed K4P4 F Sig.

  Lampiran B. Data Rataan Kerusakan Hati Berupa Steatosis

Rataaan kerusakan hati mencit (Mus musculus L.) berupa steatosis setelah

pemberian kombinasi testosteron undekanoat (TU) dan ekstrak air biji pepaya

(Carica papaya L.) (x±SD) Lama Pemberian (Minggu) Kontrol (%) Perlakuan (%)

  

T0 1,2±1,0 5,4±4,0

T1 2,4±2,0 5,6±1,4

T2 3,1±2,5 5,7±1,5

T3 1,5±0,7 6,3±1,8

T4 1,4±1,3 1,5±0,7

Hasil Uji Statistik SPSS

a. Kelompok Kontrol Tests of Normality

  .298 5 .168 .865 5 .246 .185 5 .200* .967 5 .852 .136 5 .200* .990 5 .980 .360 5 .033 .767 5 .042 .269 5 .200* .894 5 .376

  Kel.KONTROL K0 K1 K2 K3 K4 STETOSIS Statistic df Sig. Statistic df Sig.

  Kolmogorov-Smirnov

  a

  Shapiro-Wilk This is a lower bound of the true s ignificance. *.

  Lilliefors Significance Correction a.

  Test of Homogeneity of Variance

  11.259

  4 20 .000 2.239

  4 20 .101 2.239 4 5.887 .183

  11.026

  4 20 .000 Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean

  STETOSIS Levene Statistic df1 df2 Sig.

  Mann-Whitney Test Ranks

  KELOMPOK N Mean Rank Sum of Ranks STEATOSIS KONTROL

  25 19.92 498.00 PERLAKUAN

  25 31.08 777.00 Total

  50

  a Test Statistics

  STEATOSIS Mann-Whitney U 173.000 Wilcoxon W

  498.000 Z

• 2.716 As ymp. Sig. (2-tailed) .007

  a. Grouping Variable: KELOMPOK

  NPar Tests Friedman Test Ra nks a Test Statistics

  Mean Rank N

  5 K0

  4.00 Chi-Square 6.189

  K1

  3.00 df

  4 K2

  3.70 As ymp. Sig. .185 K3

  2.20

  a. Friedman Test K4

2.10 Friedman Test (Kelompok Perlakuan)

  Ra nks a Test Statistics

  Mean Rank N

  5 P0

  3.00 Chi-Square 8.000

  P1

  3.80 df

  4 P2

  3.00 As ymp. Sig. .092 P3

  3.80 a. P4 Friedman Test

  1.40

  Interval of the Difference t-test for Equality of Means

  Levene's Test for Equality of Variances t df Sig. (2-tailed) Mean

  Difference Std. Error Difference Lower Upper 95% Confidence

  Levene's Test for Equality of Variances t df Sig. (2-tailed) Mean

  Independent Samples Test 1.453 .263 -1.980 8 .083 -2.60000 1.31339 -5.62869 .42869

  Mean

  N Mean Std. Deviation Std. Error

  5 3.1000 2.53476 1.13358 5 5.7000 1.48324 .66332 kel_steatos is KONTROL PERLAKUAN K2P2

  K2P2 (T-Test) Group Statistics

  Interval of the Difference t-test for Equality of Means

  Difference Std. Error Difference Lower Upper 95% Confidence

  

Independent Samples Test

.379 .555 -2.415 8 .042 -1.32116 .54695 -2.58244 -.05989

  K0P0 (T-Test) Independent Samples Test 3.262 .109 .396 8 .702 1.30000 3.27872 -6.26074 8.86074 .396 6.816 .704 1.30000 3.27872 -6.49552 9.09552

  Std. Error Mean

  K1P1_trans N Mean Std. Deviation

  5 1.3432 .86291 .38591 5 2.6644 .86670 .38760 kel_steatos is KONTROL PERLAKUAN

  K1P1 (T-Test) Group Statistics

  Interval of the Difference t-test for Equality of Means

  Difference Std. Error Difference Lower Upper 95% Confidence

  Levene's Test for Equality of Variances

t df Sig. (2-tailed)

Mean

  K0P0 F Sig.

  Equal variances as sumed Equal variances not ass umed

• 2.415 8.000 .042 -1.32116 .54695 -2.58244 -.05988 Equal variances as sumed Equal variances not ass umed K1P1_trans F Sig.
• 1.980 6.452 .092 -2.60000 1.31339 -5.75998 .55998 Equal variances as sumed Equal variances not ass umed K2P2 F Sig.

  K3P3 (Mann- Witney Test) Ranks

  kel_steatos is N Mean Rank Sum of Ranks K3P3 KONTROL

  5

  3.00

  15.00 PERLAKUAN

  5

  8.00

  40.00 Total

  10

  b Test Statistics

  K3P3 Mann-Whitney U

  .000 Wilcoxon W

  15.000 Z

• 2.643 As ymp. Sig. (2-tailed)

  .008

  a

  Exact Sig. [2*(1-tailed .008

  Sig.)] a. Not corrected for ties.

  b. Grouping Variable: kel_s teatosis

  K4P4 (Mann- Witney Test) Ranks

  kel_steatos is N Mean Rank Sum of Ranks K4P4 KONTROL

  5

  5.20

  26.00 PERLAKUAN

  5

  5.80

  29.00 Total

  10

  b Test Statistics

  K4P4 Mann-Whitney U

  11.000 Wilcoxon W 26.000 Z

• .334 As ymp. Sig. (2-tailed)

  .738

  a

  Exact Sig. [2*(1-tailed .841

  Sig.)] a.

  Not corrected for ties.

  b.

  Grouping Variable: kel_s teatosis

  Lampiran C. Data Rataan Kerusakan Hati Berupa Degenerasi Hidrofik

Rataaan kerusakan hati mencit (Mus musculus L.) berupa degenerasi hidrofik

setelah pemberian kombinasi testosteron undekanoat (TU) dan ekstrak air biji

pepaya (Carica papaya L.) (x±SD) Lama Pemberian (Minggu) Kontrol (%) Perlakuan (%)

  T0 4,4±2,2 10,8±3,9 T1 5,0±2,9 11,7±8,4 T2 5,4±3,5 18,1±7,5 T3 3,0±2,8 12,2±2,8

T4 2,4±1,1 5,9±1,7

Hasil Uji Statistik SPSS

  Tests of Normality

  .233 25 .001 .869 25 .004 .095 25 .200* .963 25 .479

  KELOMPOK KONTROL PERLAKUAN D.HIDROFIK Statistic df Sig. Statistic df Sig.

  Kolmogorov-Smirnov

  a

  Shapiro-Wilk This is a lower bound of the true s ignificance.

• .

  Lilliefors Significance Correction a.

  Test of Homogeneity of Variance

  4.069

  1 48 .049 4.589

  1 48 .037 4.589 1 45.800 .038 4.265

  1 48 .044 Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean

  D.HIDROFIK Levene Statistic df1 df2 Sig.

• 3.749

  5 8.085

  N Chi-Square df As ymp. Sig.

  4 .058

  5 9.120

  

Test Statistics

a

  Mean Rank

  1.40 P0 P1 P2 P3 P4

  4.20

  3.60

  3.20

  2.60

  Ra nks

  Friedman Test a.

  N Chi-Square df As ymp. Sig.

  4 .089

  

Test Statistics

a

  NPar Tests Mann-Whitney Test Ranks

  .000 Mann-Whitney U Wilcoxon W Z As ymp. Sig. (2-tailed)

  25 17.78 444.50

  25 33.22 830.50

  50 KELOMPOK KONTROL PERLAKUAN Total

  D.HIDROFIK N Mean Rank Sum of Ranks

  Test Statistics a

  119.500 444.500

  D.HIDROFIK Grouping Variable: KELOMPOK a.

  Mean Rank

  NPar Tests Friedman Test Ra nks

  4.20

  3.50

  3.30

  2.10

  1.90 K0 K1 K2 K3 K4

  Friedman Test a.

• .606 7.565 .562 -2.90000 4.78853 -14.05393 8.25393 Equal variances as sumed Equal variances not ass umed K0P0 F Sig.

  N Mean Std. Deviation Std. Error

  N Mean Std. Deviation Std. Error

  5 5.0000 2.91548 1.30384 5 12.2000 2.77489 1.24097 kel_D.hidrofik KONTROL PERLAKUAN K2P2

  K2P2 (T-Test) Group Statistics

  Interval of the Difference t-test for Equality of Means

  Difference Std. Error Difference Lower Upper 95% Confidence

  Levene's Test for Equality of Variances

t df Sig. (2-tailed)

Mean

  

Independent Samples Test

.182 .681 -2.287 8 .052 -5.40000 2.36114 -10.84481 .04481

  Mean

  5 5.4000 3.54260 1.58430 5 10.8000 3.91472 1.75071 kel_D.hidrofik KONTROL PERLAKUAN K1P1

  K1P1 (T-Test) Group Statistics

  Interval of the Difference t-test for Equality of Means

  Difference Std. Error Difference Lower Upper 95% Confidence

  Levene's Test for Equality of Variances

t df Sig. (2-tailed)

Mean

  

Independent Samples Test

.156 .703 -.606 8 .562 -2.90000 4.78853 -13.94237 8.14237

  Mean

  N Mean Std. Deviation Std. Error

  5 8.8000 6.60114 2.95212 5 11.7000 8.43060 3.77028 kel_D.hidrofik KONTROL PERLAKUAN K0P0

  K0P0 (T-Test) Group Statistics

• 2.287 7.921 .052 -5.40000 2.36114 -10.85421 .05421 Equal variances as sumed Equal variances not ass umed K1P1 F Sig.

  Mean

  

Independent Samples Test

Levene's Test for Equality of Variances t-test for Equality of Means

  95% Confidence Interval of the Difference Mean Std. Error

  F Sig. t df Sig. (2-tailed) Difference Difference Lower Upper K2P2 Equal variances .295 .602 -4.000 8 .004 -7.20000 1.80000 -11.35081 -3.04919 as sumed

  Equal variances

• 4.000 7.981 .004 -7.20000 1.80000 -11.35257 -3.04743 not ass umed

  K3P3 (T-Test) Group Statistics

  Std. Error kel_D.hidrofik N Mean Std. Deviation Mean K3P3_trans KONTROL 5 .3349 .23536 .10526

  PERLAKUAN _{Independent Samples Test} 5 1.2212 .20766 .09287

  Levene's Test for

Equality of Variances t-test for Equality of Means

95% Confidence Interval of the

  Difference Mean Std. Error F Sig. t df Sig. (2-tailed) Difference Difference Lower Upper K3P3_trans Equal variances

  .178 .684 -6.314 8 .000 -.88636 .14037 -1.21006 -.56267 as sumed Equal variances

• 6.314 7.878 .000 -.88636 .14037 -1.21094 -.56179 not ass umed

  K4P4 (T-Test) Group Statistics

  Std. Error kel_D.hidrofik N Mean Std. Deviation Mean K4P4 KONTROL 5 3.0000 2.82843 1.26491

  PERLAKUAN 5 5.1000 2.79285 1.24900

  

Independent Samples Test

Levene's Test for Equality of Variances t-test for Equality of Means

  95% Confidence Interval of the Difference Mean Std. Error

  F Sig. t df Sig. (2-tailed) Difference Difference Lower Upper K4P4 Equal variances .006 .940 -1.181 8 .271 -2.10000 1.77764 -6.19924 1.99924 as sumed

  Equal variances

• 1.181 7.999 .271 -2.10000 1.77764 -6.19936 1.99936 not ass umed

  Lampiran D. Cara Pembuatan Ekstrak Air Biji Pepaya Pepaya (Carica papaya L.) Diambil biji pepaya Dicuci biji Dikeringkan dengan incubator pada suhu 50°C Dihaluskan dengan diblender dan diayak

  Bubuk halus biji pepaya Ditimbang bubuk halus biji pepaya Dimasukkan kedalam bejana yang telah diisi air Direbus hingga mendidih

  Air rebusan mendidih

Disaring dengan kertas saring

Hasil Residu

  

Dipanaskan

Rendeimen Dilarutkan dengan akuabidestilata sesuai dengan kebutuhan penelitian Ekstrak air biji pepaya

  Lampiran E. Uji Skrinning Fitokimia Biji Pepaya Terhadap Alkaloid Biji Pepaya (Carica papaya L.) Dikeringkan Dihaluskan Diambil sebanyak 3 g dan dimasukkan ke dalam erlemeyer yang berisi 100ml methanol Dipanaskan hingga ¼ volume awal Disaring Ekstrak

  Tabung I Tabung II Tabung III Tabung IV Ditetesi pereaksi Ditetesi pereaksi Ditetesi pereaksi Ditetesi

Meyer Wagner Bouchard

dragendorf Diamati Diamati Diamati Diamati Hasil Hasil Hasil Hasil

  Lampiran F. Uji Skrinning Fitokimia Biji Pepaya Terhadap Flavonoid Biji Pepaya (Carica papaya L.)

Dikeringkan

Dihaluskan Diambil sebanyak 3 g dan dimasukkan ke dalam erlemeyer yang berisi 100ml methanol Dipanaskan hingga ¼ volume awal Disaring Ekstrak

  Tabung I Tabung II Tabung III Tabung IV Ditetesi pereaksi Ditetesi pereaksi Ditetesi pereaksi Ditetesi

  3

  2 FeCl MgHCl H SO4 NaOH 10%

  Diamati Diamati Diamati Diamati Hasil Hasil Hasil Hasil

  Lampiran G. Uji Skrinning Fitokimia Biji Pepaya Terhadap Steroid Biji Pepaya (Carica papaya L.)

Dikeringkan

Dihaluskan

Diambil sebanyak 3 g dan dimasukkan ke dalam erlemeyer yang berisi 100ml n- heksan Dipanaskan hingga ¼ volume awal Disaring Ekstrak

  Tabung II Tabung I Tabung III Ditetesi pereaksi Ditetesi reagent Salkowsky Ditetesi libe

  2 CeSO4 1% H SO4 men-Bouchard

  Diamati Diamati Diamati Hasil Hasil Hasil

  Lampiran H. Uji Skrinning Fitokimia Biji Pepaya Terhadap Terpenoid Biji Pepaya (Carica papaya L.) Dikeringkan Dihaluskan Diambil sebanyak 3 g dan dimasukkan ke dalam erlemeyer yang berisi 100ml kloroform Dipanaskan hingga ¼ volume awal Disaring Ekstrak

  Tabung II Tabung I Tabung III Ditetesi pereaksi Ditetesi reagent Salkowsky Ditetesi Liber

  2 CeSO4 1% H SO4 men- bauchard

  

Diamati Diamati Diamati

Hasil Hasil Hasil

  Lampiran I. Pembuatan Preparat Histologi Hati Organ Hati dicuci dengan NaCl 0,9% difiksasi (dimasukkan ke dalam fiksatif bouin) washing (dengan menggunakan alkohol 70%, dilakukan berkali- kali hingga jernih), direndam selama 1 malam dehidrasi dilakukan dengan merendam hati ke dalam alkohol bertingkat yaitu konsentrasi 30, 40, 50, 60, 70, 80, 96 dan 100% selama 1 jam pada masing-masing konsentrasi clearing dilakukan dengan merendam hati ke dalam xylol selama 1 malam Infiltrasi Embedding dilakukan dengan meletakkan hati pada kotak berbentuk segi empat yang telah dipersiapkan sebelumnya sebagai cetakan. Dituang parafin yang telah cair ke dalam kotak tersebut, kemudian hati ditanam dan diatur posisinya lalu diberi label. Dibiarkan sampai dingin dan membeku sehingga membentuk blok parafin dan dimasukkan ke dalam freezer. Dilakukan penempelan blok-blok parafin pada holder yang terbuat dari kayu berukuran 1x1 cm yang berbentuk persegi.

  Blok Parafin

  Cutting dilakukan dengan memotong blok-blok parafin yang telah diholder pada mikrotom sehingga membentuk pita-pita parafin dengan ukuran ketebalan 6-10 µm Attaching dilakukan dengan mengambil beberapa pita parafin dengan skapel, kemudian diletakkan pada object glass, dan dicelupkan pada air dingin dan air hangat. Lalu diletakkan di atas hotplate beberapa detik untuk melekatkan pita parafin pada object glass

  Deparafinasi dilakukan dengan cara mencelupkan objek pada xylol sampai parafin habis kira-kira selama ± 15 menit Dealkoholisasi dilakukan dengan mencelupkan objek glass ke

dalam alkohol absolut, 96, 80, 70, 60, 50, 40 dan 30%

Pewarnaan sediaan hati diwarnai dengan menggunakan hematoxilin Erlich selama 3-5 menit, lalu dicuci dengan dengan air mengalir ± 3 menit, kemudian dimasukkan ke dalam alkohol 30, 50, dan 70%, lalu dimasukkan ke dalam larutan pewarna Eosin 0,5% dalam alkohol selama 1-3 menit, lalu dimasukkan ke dalam aquadest dan kemudian preparat dimasukkkan berturut-turut ke dalam alkohol 30, 40, 50, 60, 70, 80, dan 96%, alkohol absolut, dikeringkan dengan kertas tisu, selanjutnya preparat dimasukkan dalam chamber berisi xylol Mounting dilakukan dengan menutup preparat dengan Canada balsam. Diusahakan Diusahakan supaya tidak terdapat gelembung udara. Preparat diberi label

Diamati nekrosis, degenerasi hidrofik, dan steatosis

Blok Parafin

  Pita Parafin Hasil

  Lampiran J. Varietas-Varietas Pepaya (Carica papaya L.) di Indonesia.

  1. Pepaya Medan Deskripsi Morfologi: Kulit buah bewarna hijau, halus dan licin. Daging buah bewarna kuning kemerahan dan rasanya manis. Berat buah kurang lebih 1,5 kg.

a. Pepaya Medan

  2. Pepaya Bangkok Deskripsi Morfologi: Varietas pepaya Bangkok dikenal juga dengan nama pepaya Thailand. Kulit luar kasar dan tidak rata. Pepaya Bangkok berukuran lebih besar dan bentuknya lebih bulat dibandingkan pepaya Cibinong. Daging buah bewarna merah jingga dan berat buah kurang lebih 3,5 kg (Kalie, 1996).

b. Pepaya Bangkok

  3. Pepaya Cibinong Deskripsi Morfologi: Bentuk buah panjang besar dan lancip pada bagian ujungnya. Tangkai buah panjang. Daging buah bewarna merah kekuningan, rasanya kurang manis. Berat varietas pepaya kurang lebih 2,5 kg/buah (Kalie, 1996).

c. Pepaya Cibinong

  4. Pepaya Solo Deskripsi Morfologi: Ukuran buah kecil dan bentuknya mirip buah alpukat. Berat buah antara 0,4-0,1 kg/buah. Daging buah bewarna kuning, beraroma dan rasanya manis (Kalie, 1996).

d. Pepaya Solo

  5. Pepaya Burung Deskripsi Morfologi: Daging buah bewarna kuning dan berair banyak. Kulit buah bewarna hijau kekuning. Rasa asam manis (Kalie, 1996).

e. Pepaya Burung

  6. Pepaya Jinggo Deskripsi Morfologi: Daging buah bewarna merah dan berair banyak. Kulit buah bewarna kuning. Berat buah kurang lebih 1,5 kg/buah (Kalie, 1996).

  7. Pepaya California Deskripsi Morfologi: Warnanya lebih mengkilap, bentuknya agak lonjong, daging buahnya tebal, bijinya lebih sedikit, dan rasanya manis (Kalie, 1996).