RESULTS AND DISCUSSION Profil Fermentasi Rumen Sapi Pejantan Feedlot Dengan Administrasi Sikat Stimulator Mekanis Rumen (Rumenfibe ®).

11 treatments might in a tolerance rate by ∆EH condition compared with those standard even showed below than the usual. This low rate was notified as the effect of open air analysis while putted in the ∆EH electrodes then rumen liquor was oxidized that might decreased the anaerob fermentation status. Ammonia was a ruminal fermentation product that absorbed in cattle rumen as proteolytic microbe acts where amino acid deaminated Chiba, 2014. The concentration of ammonia was found in a similar value, resulted about 12.42-14.472 mM in the rumen liquor. This results was approximate with the usually found that reported by Patra 2013 on meta-analysis study, quantified at 12.9 mM in the rumen liquor of dairy and steer cattle. But Horiguchi Takahashi 2003 found a lower result than the present study i.e. about 5.3-7.1 mM. Meanwhile in contrary, Matsuyama et al. 2002 assured that the cattle administered with RMS had a lower ammonia than the control 10 mmol dL -1 vs 14 mmol dL -1 although its nitrogen retention was improved. The ammonia concentration in the present study resulted in a similar with previous studies althought there were resulted various. This result may assure that the RMS administration did not influence proteolytic microbes activity in produced ammonia. There were found difference results according to total and partial VFA concentration acetate and butyrate between RF and Control treatment. The RF treatment resulted a higher VFA than the control, i.e 142.842 mM vs 117.906 mM, influnced by the partial VFA accumulation p0.05. In partially, the acetate and butyrate concetration in RF group were higher than the control treatment, i.e. 86.1 vs 73.247 mM and 24.29 vs 19.1 mM in the rumen liquor, respectively p0.05. Instead from the acetate and butyrate, The RF treatment unable to increase the propionate concentration, resulted 24.3-32.4 mM as similar as the control. But the acetate, propionate and butyrate of the RF and control treatment had a similar proportion in percentages. Therefore, the RF and control group achieved a similar quantities on Acetic:Propionic ratio, pointed out about 2.89-2.91. The increased of total VFA status in RF treatment was resulted by acetate and butyrate concentration P0.05, even the propionate concentration tended to be higher than the control. This present study achieved better result compared with Horiguchi and Takahashi 2004 study between with and without RMS administration to Holstein steer that gave a similar quantitiy of acetate, propionate and butyrate concentration, resulted about 40 mM; 24 mM; and 26 mM in the urmen liquor. Matsuyama et al. 2002;2004 found the propionic and butyric concentration of RMS treatment increased than the control i.e. 34.3 and 20 mmol dL -1 vs 27.1 and 16.6 mmol dL -1 , respectively. But by the acetate and total VFA in RF treatment had lowered than the control by 24 hours 50 vs 58 mmol dL -1 and 100 vs 125 mM while Holstein steers fed mainly rolled barley. In the contrary, Horiguchi Takahashi 2002 found that the RMS treatment had a lower total VFA propotion than the control 61.8 vs 87.9 mmol dL -1 after 4 hours feeding and 67.3 vs 78.3 mmol dL -1 after 24 hours feeding, but produced higher total VFA proportion than the Control 89.3 vs 45 mM while fed long rice straw and high concentrate diet to Holstein steers. These previous studies were generally unidentical according to present result by their total and partial VFA concentration, such acetate,