Saran SIMPULAN DAN SARAN

p-value 0,05 selama hang time hingga jam ke-8, yang menunjukkan BAL L. rhamnosus R21 dan R25 tidak terganggu oleh keberadaan C. sakazakii YRC3a.

5.2 Saran

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Pengaruh suhu rekonstitusi 50 °C terhadap isolat BAL Uji F: ANOVA Single Factor Perubahan jumlah BAL Sumber Keragaman Jumlah Kuadrat Derajat Bebas Kuadrat Tengah F-hit F-tab Between Groups 3,12 10 0,31 34,35 2,85 Within Groups 0,09 11 0,01 Total 3,22 21 Hipotesis : Ho: µ1= µ2= µ3= µ4= µ5=µ6= µ7= µ8= µ9= µ10= µ11 Ho: Minimal Terdapat sepasang µi≠ µi’ Keputusan : Berdasarkan Hasil uji-F dapat dilihat minimal terdapat sepasang perlakuan yang memberikan pengaruh yang berbeda nyata pada taraf α=0,05 dimana FhitFtab Kode Isolat Genusgalur Penghambatan EPEC Log CFUmL Jumlah Awal No CFUmL Rekonstitusi suhu 27 °C Jumlah Akhir Nt CFUmL Rekonstitusi suhu 50 °C Perubahan Jumlah Log CFUmL Rata- rata SB A22 L. acidophilus 1,26 5,1 x 10 6 1,9 x 10 5 1,43 1,44 0,01 6,0 x 10 6 2,2 x 10 5 1,44 A23 L. rhamnosus 1,95 1,3 x 10 7 1,2 x 10 7 0,04 0,18 0,19 1,8 x 10 7 8,8 x 10 6 0,31 A24 L. rhamnosus 1,00 3,6 x 10 7 3,5 x 10 7 0,01 0,06 0,07 5,6 x 10 7 4,4 x 10 7 0,11 A27 L. rhamnosus 1,16 1,9 x 10 7 1,6 x 10 7 0,09 0,06 0,04 2,1 x 10 7 1,9 x 10 7 0,03 A29 L. rhamnosus 1,13 3,2 x 10 7 2,2 x 10 7 0,17 0,11 0,08 2,9 x 10 7 2,6 x 10 7 0,05 R14 L. rhamnosus 2,05 5,4 x 10 7 3,3 x 10 7 0,21 0,32 0,15 5,7 x 10 7 2,1 x 10 7 0,42 R21 L. rhamnosus 2,12 6,9 x 10 7 3,8 x 10 7 0,27 0,26 0,01 6,7 x 10 7 3,7 x 10 7 0,25 R23 L. rhamnosus 2,06 4,5 x 10 7 3,2 x 10 7 0,14 0,08 0,09 2,4 x 10 7 2,4 x 10 7 0,01 R25 Lactobacillus 2,06 6,9 x 10 7 4,3 x 10 7 0,21 0,18 0,05 6,3 x 10 7 4,6 x 10 7 0,14 R27 Lactobacillus 1,06 9,7 x 10 7 6,6 x 10 7 0,17 0,10 0,11 10,1 x 10 7 9,6 x 10 7 0,02 R32 L. rhamnosus 1,00 5,1 x 10 7 2,3 x 10 7 0,34 0,29 0,07 3,1 x 10 7 1,8 x 10 7 0,24 Keterangan: Air Susu Ibu; Hartanti 2010; SB= Simpangan baku Uji Lanjut: Uji pembanding berpasangan uji Duncan Perubahan jumlah BAL Duncan a b c Isolat Keputusan N Subset for alpha = 0.05 1 2 3 A24 a 2 0,06 A27 a 2 0,06 R23 a 2 0,08 R27 ab 2 0,09 0,09 A29 ab 2 0,11 0,11 A23 ab 2 0,18 0,18 R25 ab 2 0,18 0,18 R21 ab 2 0,26 0,26 R32 ab 2 0,29 0,29 R14 b 2 0,32 A22 c 2 1,44 Sig. 0,05 0,06 1,00 Means for groups in homogeneous subsets are displayed. Lampiran 2 . Kompetisi BAL asal ASI dengan C. sakazakii YRC3a dalam susu formula rekonstitusi pada suhu 27 °C selama 24 jam • Lampiran 2a . Perubahan jumlah C. sakazakii YRC3a YRC3a pada media TSAYE-SC Keterangan Jumlah Log CFUmL Δ Log CFUmL Rata- rata SB U 1 U 2 Δ U1 24-0 jam Δ U2 24-0 jam 0 jam 24 jam 0 jam 24 jam R14 vs YRC3a 2,69 7,11 2,69 7,04 4,41 4,36 4,38 0,01 R21 vs YRC3a 2,89 4,13 2,96 4,31 1,24 1,34 1,29 0,04 R23 vs YRC3a 3,06 9,89 3,02 9,91 6,83 6,89 6,86 0,02 R25 vs YRC3a 3,06 6,63 3,02 6,45 3,57 3,43 3,50 0,05 • Lampiran 2b . Perubahan jumlah pada media MRSA-AA Keterangan Jumlah Log CFUmL Δ Log CFUmL Rata- rata SB U 1 U 2 Δ U1 24-0 jam Δ U2 24-0 jam 0 jam 24 jam 0 jam 24 jam R14 vs YRC3a 7,42 10,04 7,39 10,03 2,61 2,64 2,63 0,01 R21 vs YRC3a 7,79 9,39 7,67 9,40 1,59 1,73 1,66 0,05 R23 vs YRC3a 7,41 9,89 7,30 9,92 2,48 2,61 2,54 0,05 R25 vs YRC3a 7,48 9,86 7,60 9,89 2,38 2,29 2,34 0,03 Lampiran 2c . Penghambatan C. sakazakii YRC3a Keterangan Rata-rata STDEV Penghambatan R14 vs YRC3a 4,38 0,02 -0,27 R21 vs YRC3a 1,29 0,04 2,82 R23 vs YRC3a 6,86 0,02 -2,75 R25 vs YRC3a 3,50 0,05 0,61 YRC3a 4,11 0,07 - Uji F: ANOVA Single Factor Penghambatan jumlah C. sakazakii YRC3a Sumber Keragaman Jumlah Kuadrat Derajat Bebas Kuadrat Tengah F-hit F-tab Between Groups 31,89 4 7,97 1,04E3 5,19 Within Groups 0,04 5 0,01 Total 31,93 9 Hipotesis : Ho: µ1= µ2= µ3= µ4= µ5 Ho: Minimal Terdapat sepasang µi≠ µi’ Keputusan : Berdasarkan Hasil uji-F dapat dilihat minimal terdapat sepasang perlakuan yang memberikan pengaruh yang berbeda nyata pada taraf α=0,05 dimana FhitFtab Uji Lanjut: Uji pembanding berpasangan uji Duncan Penghambatan C. sakazakii YRC3a Duncan a Kompetisi BAL vs Cronobacter N Subset for alpha = 0.05 Keputusan 1 2 3 4 5 R21 vs YRC3a a 2 1.29E0 R25 vs YRC3a b 2 3.50E0 Kontrol YRC3a c 2 4.11E0 R14 vs YRC3a d 2 4.38E0 R23 vs YRC3a e 2 6.86E0 Sig. 1.00 1.00 1.00 1.00 1.00 Means for groups in homogeneous subsets are displayed. Lampiran 3. Perubahan nilai pH pada kompetisi BAL asal ASI dengan C. sakazakii YRC3a dalam susu formula rekonstitusi Perlakuan pH 0 jam pH 24 jam YRC3a vs R14 7,7 4,5 YRC3a vs R21 7,6 4,5 YRC3a vs R23 7,6 4,4 YRC3a vs R25 7,6 4,4 Kontrol R14 7,6 4,9 Kontrol R21 7,6 4,8 Kontrol R23 7,7 4,8 Kontrol R25 7,7 4,8 Kontrol YRC3a 7,8 5,7 Lampiran 4. Pengeringan beku dan uji viabilitas BAL • KODE ISOLAT: L. rhamnosus R21 Ʃ No biomassa sel +kriogenik CFUmL Ʃ Nb setelah pembekuan CFUmL Ʃ Nt setelah pengeringan beku CFUg Pengenceran U1 U2 Pengenceran U1 U2 Pengenceran U1 U2 10 -8 TBUD TBUD 10 -7 TBUD TBUD 10 -7 TBUD TBUD TBUD TBUD TBUD TBUD TBUD TBUD 10 -9 156 135 10 -8 TBUD TBUD 10 -8 TBUD TBUD 127 100 TBUD TBUD TBUD TBUD 10 -10 17 13 10 -9 80 73 10 -9 112 185 15 14 80 68 140 145 10 -11 2 3 10 -10 8 10 10 -10 25 46 2 7 5 39 35 Ʃ 1,42.10 11 1,18.10 11 Ʃ 8,00.10 10 7,05.10 10 Ʃ 1,43.10 11 1,86.10 11 Log 11,15 11,07 Log 10,90 10,45 Log 11,16 11,27 • KODE ISOLAT: L. rhamnosus R25 Ʃ No biomassa sel +Kriogenik CFUmL Ʃ Nb setelah pembekuan CFUmL Ʃ Nt setelah pengeringan beku CFUg Pengenceran U1 U2 Pengenceran U1 U2 Pengenceran U1 U2 10 -8 TBUD TBUD 10 -7 TBUD TBUD 10 -7 TBUD TBUD TBUD TBUD TBUD TBUD TBUD TBUD 10 -9 111 140 10 -8 TBUD TBUD 10 -8 TBUD TBUD 159 110 TBUD TBUD TBUD TBUD 10 -10 11 28 10 -9 80 67 10 -9 202 212 13 24 73 76 183 226 10 -11 2 1 10 -10 8 2 10 -10 50 53 7 8 7 47 56 Ʃ 1,31.10 11 1,32.10 11 Ʃ 7,65.10 10 7,15.10 10 Ʃ 2,19.10 11 2,48.10 11 Log 11,12 11,12 Log 10,88 10,85 Log 11,34 11,39 Keterangan: Jumlah bakteri merupakan hasil rata-rata 2 kali ulangan dan duplo; No: Jumlah mikroba awal+laktosa; Nb: Jumlah mikroba setelah pembekuan; Nt: Jumlah mikroba setelah freeze driying Lampiran 5. Gambar persiapan dan proses pembuatan BAL kering beku Lampiran 5a: Proses penyalutan sel BAL Lampiran 5b: Produksi sel BAL dengan kriogenik laktosa pada medium MRSB Lampiran 5c : Proses pengeringan beku BAL Lampiran 5d : Kondisi pengaturan alat pada proses pengeringan beku BAL Lampiran 5e : Uji viabilitas BAL setelah pengeringan beku Lampiran 6 : Uji Kompetisi BAL kering beku R21 dan R25 dengan C.sakazakii YRC3a pada T= 50;60;70 °C; dan selama Hang time t= 0;2;4;6;8 jam Lampiran 6a . Data kompetisi R21 vs YRC3a pada media MRSAA-AA pada suhu rekonstitusi 50 o C Jam ke- Suhu 50 o C Rata-rata Ulangan 1 Ulangan 2 Pengenceran ∑Koloni CFUmL Log CFUmL Pengenceran ∑Koloni CFUmL Log CFUmL CFUmL Log CFUmL 10 -5 TBUD 1.43x10 8 8.16 10 -4 TBUD 1.47x10 8

8.17 1.45x10