Protein Analysis The analyses of forage quality

26 5 10 15 20 25 30 35 60 80 M g ha a a b such as 60 and 80, the forage yield required higher organic fertilizer. In 40 and 50 days of plantation, the additional 30 Mgha organic fertilizer presences the highest forage yield underneath limitation of sun availability. While, in the longer time or harvest time, it required less organic fertilizer to obtain maximum yield. In 60 days after plantation, the organic fertilizer was required for 20 Mgha. We calculated that as 31.1 of forage production was depleting, since it planted on 80 Levels of shade Figure 9. Figure 9. The main effect levels of shade on forage yield P. purpureum. Subscripts with the same letter showed the significant different test by Least Square Determination LSD in P0.05. Moreover, the Analysis of Variance ANOVA result showed there was significance different on the Levels of shade P0.05. From figure 9, it could be seen that forage yield showed significant differences on levels of shade. The result indicated that the less number of forage yield was produced in higher levels of shade. It gained that the highest amount was obtained on 0 levels of shade as 30.50 Mgha. Furthermore, we also obtained the interaction both the organic fertilizer and defoliation management due to forage yield Figure 10. 27 5 10 15 20 25 30 35 40 40d 50d 60d 40d 50d 60d 40d 50d 60d 10 Mgha 20 Mgha 30 Mgha M g ha Figure 10. The interaction both fertilization and defoliation management on forage yield P.purpureum. In figure 10, the interaction occurred as the impact of additional organic fertilization and defoliation management on forage yield. Furthermore, it could be understood that the additional fertilizer, compacted with defoliation management. As in the early growing stage on the plant that required additional fertilizer to support plant growth. In the longer time of defoliation management, the amount of fertilizer needed slowly decreasing. We also observed the influenced of treatments on S. splendida. In table 8 was showing the forage yield S. splendida influenced by levels of shade, organic fertilizer and defoliation management. Table 8. The Measurement fresh weight production Mgha of S. splendida on levels of shade, organic fertilizer and defoliation management treatments Levels of shade Organic Fertilizer Mgha Defoliation management 40d 50d 60d 30 27.2±0.7 26.1±1.7 18.8±3.4 20 20.3±0.8 31.3±0.7 23.5±5.3 10 21.2±2.5 15.2±0.7 17.5±1.9 60 30 20.5±5.6 27.5±1.1 20.2±0.8 20 23.3±5.8 28.7±5.0 15.5±6.0 10 21.3±5.3 28.7±2.7 13.7±4.0 80 30 18.6±2.9 22.2±1.0 12.5±2.6 20 18.1±2.1 11.5±4.4 18.3±3.2 10 16.6±1.9 25.2±4.0 14.4±2.2 Table 8 described the forage yield measurement of S. splendida. It could be seen from table 8 that the average of highest forage yield production was 24.73 Mgha. It was found in 50 days after plantation. Unlike P. purpureum where 28 5 10 15 20 25 30 40 50 60 M g ha days 5 10 15 20 25 30 35 M g ha shading highly influenced to forage yield. In S. splendida, within the longer time of defoliation management, it required less organic fertilizer. It could be seen in Table 8 that the optimum forage yield for 0 levels of shade in 40 days after plantation for 30 Mgha, and with the longer time of defoliation management 50 and 60 days after plantation, the requirement of organic fertilizer was reducing for 20 Mgha. The result quite different with the influence of the limitation of sun availability, whereas in 60 levels of shade showed the higher organic fertilizer requirement for 20 Mgha for 40 and 50 days after plantation. The organic fertilizer was required higher for 30 Mgha for the longer time defoliation management 60 days after plantation. In this study, defoliation management has a significance effect due to forage yield Figure 11. Figure 11. The main effect defoliation management on forage yield Mgha S. splendida. Subscripts with the same letter showed the significant different test by Least Square Determination LSD in P0.05. Figure 11 described the influence of defoliation management due to fresh weight of S. splendida. It could be seen that the different time of days after plantation has lead the different amount of fresh weight production. In this study, there was significance different on 60 days defoliation management p0.05. It also found that S. splendida did not show the direct effect regarding to shading effect. However, the influenced of levels of shade has seen as the interaction with the additional organic fertilizer Figure 12. a a ab