Soil and Fertilizer Analysis Measurement

21 solution. Add 2 to 3 drops of tributyl citrate or other anti foam agent to digestion flask to reduce foaming. Add another 0.5 to1.0 galundum granules. Slowly down side of flask, add sufficient 45 sodium hydroxide solution approximately80 
 mL to make mixture strongly alkali. Do not mix until after flask is connected to distillation 
 apparatus or ammonia will be lost. Immediately connect flask to distillation apparatus and distill at about 7.5 boil rate 
 temperature set to bring 250 ml at 25 o C to boil in 7.5 min, until at least 150 ml, distillate was collected in titrating flask. Remove digestion flask and titrating flask from unit, rinsing the condenser tube with distilled 
 water as the flask is being removed. Titration Titrate excess acid with standard sodium hydroxide solution to orange endpoint color change from red to orange to yellow and record volume to nearest 0.01 mL VNaOH. Titrate the reagent blank B similarly. The calculation of N : [ ] V NaOH = mL standard NaOH needed to titrate sample V HCl = mL standard HCl pipetted into titrating flask for sample V NaOH = Normality of NaOH
 V HCl = Normality of HCl V BK = mL standard NaOH needed to titrate 1 mL standard HCl minus B B = mL standard NaOH needed to titrate reagent blank carried through method and distilled into 1 mL standard HCl 1.4007 = milliequivalent weight of nitrogen x 100 W = sample weight in grams Percent Crude Protein CP CP DM basis= N DM basis X F Where F = 6.25 for all forages and feeds except wheat grains 22

3.4.4.5 Fiber Analysis

Fiber analysis was conducted based on AOAC method. The understanding principle of this calculation was the organic material that not soluble in liquid concentrated acid and concentrated base heating for 30 minutes. The rest material remaining was fiber. It would be burn into 600 O C, and stated as the fiber content . Fiber Where : x = sample weighed a = the weight of paper filter y = sample weight of the cup after heated on liquid concentrated acid and Concentrated base. Z = sample after heated on 600 O C

3.4.5 Statistical Analysis

Plant production and nutrient analyses data were tested for significant differences using randomize block complete ANOVA. Least significance difference LSD post hoc tests were conducted to compare means between treatments. Data were analyzed by using StatView, SAS Institute Inc. Hypothesis 1. The Hypothesis of treatment factors due to levels of shade H0 = ̅̅̅̅= ̅̅̅̅= ̅̅̅̅ H1 = At least there was a level shade treatment resulting in the effect 2. The Hypothesis of treatment factors due to organic fertilizers H0 = ̅̅̅̅= ̅̅̅̅= ̅̅̅̅ H1 = At least there was an organic fertilizers treatment has an effect 3. The Hypothesis of treatment factors due to Blocking‘s components defoliation management H0 = ̅̅̅̅= ̅̅̅̅̅̅̅ ̅̅̅̅ H1 = ̅̅̅̅ ≠ ̅̅̅̅ ≠ ̅̅̅̅ 4. The Hypothesis of interaction both A and B