V. KESIMPULAN DAN SARAN
5.1 Kesimpulan
Penggunaan vaksin DNA dengan dosis 2.5, 7.5 dan 12.5 µg100µ l selama
masa vaksinasi memberikan pengaruh terhadap beberapa parameter hematologi yaitu total leukosit, persentase limfosit dalam darah dan indeks fagositik.
Sedangkan pada masa uji tantang dengan virus KHV pengenceran 10
-3
parameter darah yang terlihat berpengaruh nyata adalah limfosit, monosit dan indeks
fagositik. Pada akhir pengamatan, perlakuan C dosis 12.5 µg100µ l menunjukan persentase limfosit tertinggi, total rataan eritrosit tertinggi dan persentase monosit
yang lebih cepat turun dari kedua perlakuan dosis lainnya, sehingga dapat disimpulkan bahwa vaksin DNA dengan dosis 12.5 µg100µ l memberikan
efektifitas respon tanggap kebal terbaik dibandingkan dengan kedua dosis yang
lain.
5.2 Saran
Diperlukan suatu kajian mengenai penambahan dosis vaksin DNA untuk mengetahui dosis optimum vaksin, sehingga diperoleh respon kekebalan tubuh
ikan lebih baik.
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Lampiran 1. Kadar Hemoglobin, Kadar Hematokrit, Total Eritrosit dan Total Leukosit
Sampling hari ke- sampel
7 14
21 Hb
g Ht
Total eritrosit
Total leukosit
Hb g
Ht Total
eritrosit Total
leukosit Hb
g Ht
Total eritrosit
Total leukosit
A1 11,6
29 1370000
42150 9
23,5 1560000
42100 12
27 1750000
27350 A2
9 40,5
1660000 29500
7,8 23
1340000 28050
6 11
1010000 47950
A3 9,4
36 1360000
59600 11
31 1240000
30700 8,4
25 1170000
59100 B1
10 36
880000 14350
9,2 22,5
1780000 33700
8,5 31,5
1320000 74250
B2 10,6
42 1220000
46200 8,2
20 1220000
47500 11
18 1380000
80100 B3
9 40
1340000 46650
9,2 29
1300000 50900
6,7 19,5
830000 49800
C1 6
39 1030000
60950 11
33 1620000
47250 8,3
29 1460000
62550 C2
9,2 33
990000 60150
10,4 33
1170000 46650
7,4 31
1510000 44350
C3 7,4
40 1830000
55450 8,2
20 1140000
33450 11,2
35 1290000
55500 K1
9 33
1150000 24850
8,6 25
1740000 50550
9,4 31
1630000 35550
K2 6,6
31 1610000
36900 7,8
22 900000
22000 8,4
25 1530000
36850 K3
11 32
1830000 21150
10,2 29
910000 21350
9,6 34
1580000 28750
28 35
42 A1
8 31
1240000 81000
8 33
1480000 86750
11.2 34
1240000 92300
A2 7.2
25 1490000
87900 10
29 1790000
83200 12.4
33 1520000
109250 A3
6.6 25.5
1780000 89100
6 23.5
1320000 78150
11.4 24
1420000 99800
B1 6
31 1220000
74650 5.8
14 1020000
77800 11.4
33 1800000
125950 B2
6.6 25
1250000 54950
11 31
1460000 83800
10 26
1150000 139300
B3 9
25.5 1030000
91450 10.2
26.5 1200000
85250 11.6
20.5 1060000
116800 C1
7.2 20
1020000 43550
6 21
980000 75700
10.4 22
860000 90950
C2 8.2
31 1070000
55150 9
27 1650000
84450 10.6
34 1340000
77100 C3
6 12
850000 59950
10 25.5
1900000 110250
10.2 20
1330000 135250
K1 7
26 1390000
55550 8.2
15 1550000
63450 9.8
30 1330000
70950 K2
7.4 22
1600000 32300
10 22
1630000 84950
10 29
1200000 87050
K3 7
25 1100000
43000 8
28 1050000
84050 8.2
14 1300000
69850
49 56
63 A1
9.1 29
910000 26100
11.6 29
1160000 27300
8 20
1250000 42250
A2 10.4
35 1720000
21550 14.4
25 1970000
47950 11
29 1240000
46400 A3
8 25
1420000 33950
8.8 24
1210000 35350
11.2 30
1110000 51350
B1 8.2
26 1280000
53500 8.8
32 1150000
26600 11.2
21 1040000
46750 B2
8.4 31
1620000 48800
8.8 23
940000 18300
10 27
1000000 55500
B3 8.4
26 990000
37100 8.4
27 1560000
51500 7.2
15 1020000
49350 C1
9 25
1530000 52700
10.4 29
1160000 35050
9.4 26
1140000 48350
C2 11
32 1180000
48300 11.2
33 2300000
25250 10.2
26 1290000
53550 C3
11 35
1670000 31600
8 30
1140000 38650
12 39
1610000 53750
K1 10
30 1100000
55050 11
33 1440000
49800 10.8
20 660000
43500 K2
8.5 28
1030000 47400
9.4 27
1620000 30300
11.4 25
650000 46200
K3 6
13 1020000
35100 11.2
25.5 1470000
54800 13
25 820000
53450 70
C1 4
35 1840000
55750 C2
10 34
1520000 59700
C3 11.6
21.5 1690000
57400
Lampiran 2. Differensial Leukosit a.
Persentase Limfosit
Perlakua n
Ulanga n
Masa Vaksinasi Masa Uji Tantang
7 14
21 28
35 42
49 56
63 70
A 1
86 89
90 87
89 94
82 83
80 2
89 90
92 81
96 95
81 80
81 3
85 90
89 87
89 92
70 82
79 rata2
86.6 7
89.6 7
90.3 3
85 91.3
3 93.6
7 77.6
7 81
80 B
1 93
87 91
78 91
98 75
85 80
2 86
93 90
83 89
93 93
80 82
3 93
93 91
65 96
95 70
86 76
rata2 90.6
7 91
90.6 7
75.3 3
92 95.3
3 79.3
3 83.6
7 79.3
3 C
1 91
95 89
90 95
95 84
90 90
80 2
90 90
88 88
89 97
77 84
93 83
3 93
93 92
84 90
90 80
84 91
84 rata2
91.3 3
92.6 7
89.6 7
87.3 3
91.3 3
94 80.3
3 86
91.3 3
82.3 3
K 1
91 87
97 91
91 95
81 85
75 2
89 88
95 90
96 94
76 81
76 3
91 86
91 93
92 92
80 87
78 rata2
90.3 3
87 94.3
3 91.3
3 93
93.6 7
79 84.3
3 76.3
3
b. Persentase Monosit
Perlakua n
Ulanga n
Masa Vaksinasi Masa Uji Tantang
7 14
21 28
35 42
49 56
63 70
A 1
4 6
9 1
6 2
8 12
12 2
9 7
3 8
1 2
7 14
14 3
9 8
8 4
4 4
20 7
7 rata2
7.33 3
7 6.66
7 4.33
3 3.66
7 2.66
7 11.6
7 10.5
11 B
1 5
11 5
8 4
1 11
5 12
2 10
4 5
6 6
3 3
10 11
3 2
5 8
13 3
2 23
4 14
rata2 5.66
7 6.66
7 6
9 4.33
3 2
12.3 3
6.33 3
12.3 3
C 1
5 4
7 3
3 2
10 6
5 11
2 9
8 7
3 5
1 13
9 4
11 3
4 4
4 12
9 3
15 10
4 10
rata2 6
5.33 3
6 6
5.66 7
2 12.6
7 8.33
3 4.33
3 10.6
7 K
1 4
6 2
4 6
2 4
10 10
2 9
4 3
5 3
2 15
7 9
3 4
9 4
5 6
4 11
10 10
rata2 5.66
7 6.33
3 3
4.66 7
5 2.66
7 10
9 9.66
7
c. Persentase Neutrofil
Perlakua n
Ulanga n
Masa Vaksinasi Masa Uji Tantang
7 14
21 28
35 42
49 56
63 70
A 1
10 5
1 12
5 4
10 5
8 2
2 3
5 11
3 3
12 6
5 3
6 2
3 9
7 4
10 11
14 rata2
6 3.33
3 3
10.6 7
5 3.66
7 10.6
7 8.5
9 B
1 2
2 4
14 5
1 14
10 8
2 4
3 5
11 5
4 4
10 7
3 5
2 1
22 1
3 7
10 10
rata2 3.66
7 2.33
3 3.33
3 15.6
7 3.66
7 2.66
7 8.33
3 10
8.3 33
C 1
4 1
4 7
2 3
6 4
5 9
2 1
2 5
9 6
2 10
7 3
6 3
3 3
4 4
1 7
5 6
5 6
rata2 2.66
7 2
4.33 3
6.66 7
3 4
7 5.66
7 4.3
33 7
K 1
5 7
1 5
3 3
15 5
15 2
2 8
2 5
1 4
9 12
15 3
5 5
5 2
2 4
9 3
12 rata2
4 6.66
7 2.66
7 4
2 3.66
7 11
6.66 7
14
Lampiran 3. Indeks Fagositik
Perlakukan Ulangan Masa sebelum infeksi hari
Masa uji tantang hari 21
28 35
42 49
56 63
70 A
1 9
11 10
8 23
21 16
2 8
14 14
9 24
19 17
3 6
13 11
11 22
17 18
R 7.67
12.67 11.67 9.33
23 19
17 B
1 9
18 14
10 24
22 18
2 8
17 16
10 23
20 17
3 9
15 10
11 24
19 20
R 8.67
16.67 13.33 10.33
23.67 20.33 18.33
C 1
10 14
15 10
23 15
9 14
2 8
12 14
11 25
20 10
11 3
12 15
13 12
24 16
11 13
R 10
13.67 14
11 24
17 10
12.67 K
1 8
9 8
7 20
21 14
2 6
7 7
6 21
20 19
3 8
9 9
8 19
17 18
R 7.33
8.33 8
7 20
19.33 17
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