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LAMPIRAN
Lampiran 1 Kromatogram standar asam amino pada HPLC
Lampiran 2 Kromatogram asam amino daging lintah laut pada HPLC
Lampiran 3
K
romatogram asam amino jeroan lintah laut pada HPLC
Lampiran 4 Contoh perhitungan asam amino glutamat pada daging lintah laut
g sampel
Bobot 100
x BM
FP x
C x
standar area
Luas sampel
area Luas
amino asam
i Konsentras
µ
x
=
130445 100
x 2
, 147
x 10
x 5
x 1376498
534833 amino
asam i
Konsentras =
Konsentrasi asam amino glutamat pada daging lintah laut = 2,192
Lampiran 5 Kromatogram standar asam lemak pada GC
Lampiran 6 Kromatogram asam lemak daging lintah laut pada GC
Lampiran 7 Kromatogram asam lemak jeroan lintah laut pada GC
Lampiran 8 Contoh perhitungan asam lemak lenolenat pada daging lintah laut 100
x pelarut
i Konsentras
- 100
sampel i
Konsentras lemak
mgg lemak
Asam =
100 x
2551 ,
97 100
5739 ,
lemak mg
lemak Asam
− =
Konsentrasi asam lemak lenolenat pada daging lintah laut = 20,908
Lampiran 9 Hasil analisis sidik ragam aktivitas antioksidan ekstrak kasar lintah
laut
Perlakuan Ulangan 1
Ulangan 2 Nilai IC50
SD
Kloroform daging 515,479
458,987 487,233
39,946 Kloroform jeroan
1327,750 1211,604
1269,677 82,128
Etil asetat daging 4407,455
2519,650 3463,552
1334,879 Etil asetat jeroan
2655,211 2397,000
2526,105 182,582
Etanol daging 452,651
429,586 441,118
16,309 Etanol jeroan
871,955 792,895
832,425 55,904
Tabel Sidik Ragam
Sumber keragaan
db Jumlah kuadrat
Kuadrat tengah Fhit
Ftab Perlakuan
5 15.107.987,21
3.021.597,44 A
2 13.463.841,36
6.731.920,68 22,11
5,14 B
1 18.613,08
18.613,08 0,06
5,99 AB
2 1.625.532,78
812.766,39 2,67
5,14 Sisa
6 1.826.971,21
304.495,20 Total
11 16.934.958,42
A : jenis pelarut B : bagian lintah laut
AB : interaksi jenis pelarut dengan bagian lintah laut
Uji lanjut BNT t hit =
945,788 A
B C
D E
F A
782,444 2976,319
2038,872 46,115
345,192 B
2193,875 1256,428
828,559 437,252
C 937,447
3022,434 2631,127
D 2084,987
1693,680 E
391,307 F
Lampiran 10 Hasil analisis sidik ragam aktivitas antioksidan masing-masing fraksi
Perlakuan Nilai IC50
SD Ulangan 1
Ulangan 2 rata-rata
F1 274.815
267.857 271.336
4.920 F2
250.690 236.774
243.732 9.840
F3 229.697
193.846 211.772
25.350 F4
162.321 174.314
168.318 8.480
F5 162.321
139.516 150.919
16.126 F6
173.846 170.577
172.212 2.312
Tabel sidik ragam
Sumber keragaan
dB Jumlah kuadrat
Kuadrat tengah Fhit
Ftab Perlakuan
5 22538,153
4507,630 24.566
4.390 Sisa
6 1100,958
183,493 Total
11 23639,109
Uji lanjut BNT t hit =
23,217 A
B C
D E
F A
27,604 59,564
103,018 120,417
99,124 B
31,960 75,414
92,813 71,520
C 43,454
60,853 39,560
D 17,399
3,894 E
21,293 F
Lampiran 11 Kromatogram GC-MS secara umum senyawa pada lintah laut
Lampiran 12 Hasil identifikasi senyawa galoksolid lintah laut pada GS-MS
Lampiran 13 Hasil identifikasi senyawa dibutil ftalat lintah laut pada GS-MS
Lampiran 14 Hasil identifikasi senyawa dioktil ftalat lintah laut pada GS-MS
Lampiran 15 Hasil identifikasi senyawa oleilamida lintah laut pada GS-MS
Lampiran 16 Hasil identifikasi senyawa erusilamid lintah laut pada GS-MS
Lampiran 17 Hasil identifikasi senyawa skualen lintah laut pada GS-MS
ABSTRACT HAFILUDDIN
. Extraction and Identification of Bioactive Compounds Sea Slug
Discodoris sp. as an antioxidant. Supervised by TATI NURHAYATI dan NURJANAH
One of the commodities produced from the ocean that have a bioactive compounds is a sea slug Discodoris sp.. That is very interesting to study mainly
deals with the nature of both chemical and biochemical characteristics and their use for the field of food and health. The purpose of this study is to determine the
nutrient content of sea slug, determine antioxidant activity, and determine the bioactive compounds of sea slug. The experiment was conducted with several
stages: sample preparation, extraction bioactive compound, fractionation by TLC and identification of compounds by GC-MS. Sea slug from the island of Madura
Pamekasan potential as a source of protein, fat, and minerals. Sea slug has esensial amino acids amount to 5,57 was dominated by leucin of 1,42.
Nonesensial amino acids amount to 6,54 wich was dominated by glutamic acid 2,19. Saturated fatty acids amounted to 27.53 of sea slug was dominated by
palmitic C16:0 which was 13.36. Unsaturated fatty acids amounted to 34.66, which was dominated by the essential fatty acids linolenic C18:3, n-3 20.91.
The highest yield of the crude extract was ethanol of 6,97 and contained alkaloid, steroids, saponin, phenols, carbohydrates and reducing sugar compound.
The bioactive compounds in the meat of sea slug with ethanol solvent was galoxolide, dibuthyl phthalate, di-n-octhyl phthalate, oleic acid amide,
erucylamide, squalene and has an IC
50
best antioxidant activity in fraction 5 at 150.92 ppm.
Key words: antioxidant, bioactive compounds, sea sluge Discodoris sp.
1 PENDAHULUAN