Class : Asteroidea
Order : Valvatidae
Suborder : Ophiurina
Family : Ophidiasteridae
Genus : Fromia
Species : Fromia milleporella
Clark and Rowe, 1971; Erhardt and Moosleitner, 1999; [13]
3. RESEARCH METHODS
3.1. Time and Location
This research was conducted in Marine Habitat Lab, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University FPIK-IPB on December
2010-November 2011.
3.2. Materials
Materials that was being used in this study are shown in Table 1 and the pictures of materials are shown in Appendix 1.
Table 1. Materials that were used in this study.
Materials Brand Type
Specification Functions
Seawater Salinity: 33-
35 ‰ From Teluk
Jakarta, then filtered in
Gelanggang Samudera
Seaworld, Ancol, Jakarta Arafat,
pers. comm., 2011
Freshwater Aqua
1 galon = 19 L Reduce salinity
Food Fresh shrimp for
Ophiuroid. Shrimp pellet or
small piece of fresh shrimps for
starfish. Feed the
organisms
Refractometer Atago SMill-E
Hand-held refractometer,
Made in Japan Salinity 0~100 ‰
Measure salinity
Thermocycler with jacket tank
customary designed by British
Antarctic Survey Grant LTD20G
-30 C to 100
C
2.3 KW
220-240 V
50-60 Hz
Tank dimension:
-
Inner: 40 x 75 x 31 cm
-
Outer: 54 x 90 x 39 cm
Increase or decrease
temperature during
experiment.
Camera Documentation
Table 1. Materials that were used in this study continued.
Materials Brand Type
Specification Functions
Thermometer Blue Gizmo
BG43A electronic or
digital thermometer
-4 C to 60
C
40 F to 140
F Measure
temperature
Standard manual
thermometer 11
C -10 C to
110 C
Digital balance IKEA Ordning
Max. 3000 g; d = 1 g
Max. 6 lbs 9 oz; d = ¼ oz
Measure weight
“Lucky” pocket scale
Digital scale
Capacity: 200 g x 0.01 g
Calliper
15 cm max.
0.05 cm accuracy
6 inches max.
1128 inches accuracy
Mesure length or diameter
Heater adjustable temperature
MP Heater micro
Processor
With temperature
setting
Company: Aquatic Nature
300 W
220-240 V
50-60 Hz
20-35 C
Heating the seawater on
separate tank plastic tank for
water exchange in experiment
tank.
Ammonia Aquarium Test Strips
API Aquarium Pharmaceuticals
For fresh and saltwater
aquarium
Ammonia ppm mgL: 0
– 0.5 – 1.0 – 3.0 – 6.0
Measure ammonia
pH Aquarium Test Strips
API Aquarium Pharmaceuticals
For fresh and saltwater
aquarium
PH freshwater: 6.0
– 6.5 – 7.0 – 7.5
– 8.0 – 8.5 – 9.0
PH saltwater: 6.0
– 6.5 – 7.0 – 7.5
– 8.0 – 8.5 – 9.0
Measure pH