Figure 26. Number of not responding organisms from upper thermal limit experiment daily increase of Ophiomastix annulosa red ophiuroid,
accumulatively. Experiment was done on July 13
th
-22
nd
2011.
4.1.2.2.2. Ophiarachna incrassata green Ophiuroids
Green ophiuroid Ophiarachna incrassata during daily increase temperature had an upper thermal limit 100 mortality or not responding of
34.0 C; the starting temperature was 26.0
C Table 10, Figure 27. The organisms reached their 50 mortality not response at more than 33
C Figure 28. Condition of the organisms on the sixth day, the organisms became weak,
did not want to eat, and showed wounded discs 32.0 C Appendix 17. At the
end of experiment the organisms showed wounded discs and none of the organisms could turn. All organisms were already at the bottom of the
experimental tank.
1 2
3 4
5 6
7 8
9 10
1 2
3 4
5 6
7 8
9
N u
m b
e r
o f n
o t
re sp
o n
d in
g
o rg
an ism
s
Time day
Ophiomastix annulosa- July 13th-22nd 2011
Experiment n=10 Control n=8
Temp. C
34.0 35.0
26.2 27.2
28.3
29.1 35.0
31.1
Table 10. Data of upper thermal limit experiment daily increase of Ophiarachna incrassata green ophiuroid. Experiment was done on December 14
th
- 22
nd
2010.
Date Experiment
temperature C
Control temperature
C Number of
not response organisms
experiment n=8
Number of not response
organisms control
n=3
14122010 26.0
25.3 15122010
27.7 25.5
16122010 27.9
24.8 17122010
28.9 25.1
18122010 30.0
24.8 19122010
31.2 25.0
20122010 32.0
25.2 21122010
33.6 24.9
1 22122010
34.0 24.8
7
Figure 27. Temperature rise during upper thermal limit daily increase experiment of Ophiarachna incrassata Green Ophiuroids.
Experiment was done on December 14
th
-22
nd
2010.
24.0 25.0
26.0 27.0
28.0 29.0
30.0 31.0
32.0 33.0
34.0 35.0
36.0 37.0
38.0 39.0
40.0 41.0
42.0
1 2
3 4
5 6
7 8
Tem p
e ratu
re
Time day
Ophiarachna incrassata -December 14th-22nd 2010
Experiment Control
Figure 28. Number of not responding organisms from upper thermal limit experiment daily increase of Ophiarachna incrassata green
ophiuroid, accumulatively. Experiment was done on December 14
th
- 22
nd
2010.
4.1.2.2.3. Fromia milleporella red starfish
Red starfish Fromia milleporella during daily increase temperature had an upper thermal limit 100 mortality or not responding of 33.0
C; the starting temperature was 27.0
C Table 11, Figure 29. The organisms reached their 50 mortality not response at more than 32
C Figure 30. The first mortality happened on the second day. Condition of the organisms on the third day, the
organisms became weak 30.0 C Appendix 27. At the end of experiment, all of
the organisms showed mortality, wounded discs or arms destroyed or degraded, and none of the organisms could turn. All organisms were already at
the bottom of the experimental tank and lost its attachment capability.
1 2
3 4
5 6
7 8
9 10
1 2
3 4
5 6
7 8
N u
m b
e r
o f n
o t
re sp
o n
d in
g
o rg
an ism
s
Time day
Ophiarachna incrassata -December 14th-22nd 2010
Experiment n=8 Control n=3
Temp. C
26.0 34.0
33.6 32.0
33.6 34.0
30.0 27.9
