Fromia milleporella red starfish

Table 12. Data of lower thermal limit experiment hourly decrease of Ophiomastix annulosa red ophiuroid. Experiment was done at 06:00-20:00 on September 22 nd 2011. Time Experiment temperature C Control temperature C Number of not response organisms experiment n=10 Number of not response organisms control n=4 06:00 27.0 25.0 07:00 26.0 25.6 08:00 25.0 25.6 09:00 23.8 25.6 10:00 22.0 25.7 11:00 20.9 25.8 12:00 20.0 25.8 13:00 19.7 25.8 14:00 19.0 25.8 15:00 18.2 25.9 16:00 17.4 25.9 17:00 16.3 26.0 18:00 15.2 26.0 19:00 14.0 26.0 1 20:00 12.8 25.9 9 Figure 31. Temperature rise during lower thermal limit hourly decrease experiment of Ophiomastix annulosa red ophiuroid. Experiment was done at 06:00-20:00 on September 22 nd 2011. 12.0 13.0 14.0 15.0 16.0 17.0 18.0 19.0 20.0 21.0 22.0 23.0 24.0 25.0 26.0 27.0 28.0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Tem p e ratu re C Time hour Ophiomastix annulosa -September 22nd 2011 Experiment Control Figure 32. Number of not responding organisms from lower thermal limit hourly decrease experiment of Ophiomastix annulosa red ophiuroid, accumulatively. Experiment was done at 06:00-20:00 on September 22 nd 2011. 4.1.2.4. Lower salinity limit hourly decrease 4.1.2.4.1. Ophiomastix annulosa red opiuroids Red ophiuroid Ophiomastix annulosa during hourly decreased salinity had a lower salinity limit 100 mortality or not responding of 20.5; the starting salinity was 36 Table 13, Figure 33. The organisms reached their 50 mortality not response at less than 22 Figure 34. Condition of the organisms on the seventh hour, the organisms became weak and slow movement 29. On the ninth hour, some of the organisms showed little movement, slow turning, and stiffness 27. On the twelfth hour, one of the organisms did not respond anymore 24.5 Appendix 13. At the end of experiment the organisms showed weakness, stiffness, and none of the organisms could turn. The arms of the organisms were all stiff and curled. All organisms were already at the bottom of the experimental tank. The temperature in experiment and control tank was more or less stable during experiment Figure 35. 1 2 3 4 5 6 7 8 9 10 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 N u m b e r o f n o t re sp o n d in g o rg an ism s Time hour Ophiomastix annulosa -September 22nd 2011 Experiment n=10 Control n=4 Temp. C 27.0 12.8 15.2 19.0 17.4 12.8 14.0 25.0 22.0 20.0 Table 13. Data of lower salinity limit experiment hourly decrease of Ophiomastix annulosa Red Opiuroids. Experiment was done at 05:00-21:00 on November 8 th 2011. Time Experiment salinity Control salinity Experiment temperature C Control temperature C Number of not response organisms experiment n=7 Number of not response organisms control n=5 05:00 36 36 26.3 25.7 06:00 35 36 26.5 26.0 07:00 34 36 26.3 26.0 08:00 33 36 26.3 26.0 09:00 32 36 26.3 26.0 10:00 31 36 26.2 26.1 11:00 30.5 36 26.1 26.1 12:00 29 36 26.1 26.2 13:00 28.5 36 26.2 26.3 14:00 27 36 26.0 26.2 15:00 26.5 36 26.0 26.2 16:00 25 36 26.0 26.2 17:00 24.5 36 26.0 26.1 1 18:00 23 36 26.0 26.0 1 19:00 22.5 36 26.0 26.0 20:00 22 36 26.0 26.0 21:00 20.5 36 26.0 25.9 5 Figure 33. Lower salinity limit experiment hourly decrease of Ophiomastix annulosa red opiuroid. Experiment was done at 05:00-21:00 on November 8 th 2011. 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 S al in ity Time hour Ophiomastix annulosa -November 8th 2011 Experiment Salinity