Receiving organisms and acclimation

Table 2. Temperature and salinity limit of selected tropical Ophiuroidea Ophiomastix annulosa, Ophiarachna incrassata, Ophiocoma cf.dentata and Asteroidea Fromia milleporella continued Organisms Thermal Limit C 100 not responding Salinity Limit psu 100 not responding Upper Lower Decrease Hourly Hourly repeat Daily Hourly Hourly Ophiocoma cf. dentata nE: 10 TE1: 26.8 TE2: 40.5 nC: 10 TC1: 25.3 TC2:26.0 nE: 7 SE1: 36 SE2: 13 nC: 3 SC1: 36 SC2: 35 Fromia milleporella nE: 7 TE1: 26.3 TE2: 35.8 nC: 3 TC1: 25.6 TC2: 26.2 nE: 10 TE1: 26.0 TE2: 35.0 nC: 10 TC1: 25.0 TC2: 25.9 nE: 10 TE1: 27.0 TE2: 33.0 nC: 4 TC1: 25.0 TC2: 25.5 Note: TE1: temperature in experiment tank when experiment started TE2: temperature in experiment tank when all of the organisms not responding anymore SE1: salinity in experiment tank when experiment started SE2: salinity in experiment tank when all of the organisms were not responding anymore nE: number of organisms being used in experiment TC1: temperature in control tank when experiment started TC2: temperature in control tank when the experiment finished when all of the organisms in experiment tank were not responding anymore SC1: salinity in control tank when experiment started SC2: salinity in control tank when the experiment finished when all of the organisms in experiment tank were not responding anymore nC: number of organisms being used as control 4.1.2.1. Upper thermal limit hourly increase 4.1.2.1.1. Ophiomastix annulosa red ophiuroid Red ophiuroid Ophiomastix annulosa during hourly increase temperature had an upper thermal limit 100 mortality or not responding of 36.5 C and 37.0 C on the repetition; the starting temperature was 26.0 C Table 3 and 4, Figure 13 and 15. The organisms reached their 50 mortality not response at more than 36 C Figure 14 and 16. The organisms started to get weak at 35.0 C ninth hour and 33.0 C sixth hour, repeat experiment Appendix 5 and 7. Condition of the organisms at the end of both experiments: the arms were more or less straight and loose and could not turn. All organisms were already at the bottom of the experimental tank. Table 3. Data of upper thermal limit experiment hourly increase of Ophiomastix annulosa red ophiuroid. Experiment was done at 05:00-16:00 on April 19 th 2011. Time Experiment temperature C Control temperature C Number of not response organisms experiment n=10 Number of not response organisms control n=8 05:00 26.0 25.0 06:00 26.9 25.0 07:00 27.8 25.0 08:00 29.0 25.5 09:00 30.0 26.0 10:00 31.0 26.0 11:00 32.0 25.5 12:00 32.9 25.8 13:00 33.9 25.5 14:00 34.9 25.8 15:00 35.9 25.9 5 16:00 36.5 25.9 5