Receiving organisms and acclimation
Table 2. Temperature and salinity limit of selected tropical Ophiuroidea
Ophiomastix annulosa,
Ophiarachna incrassata,
Ophiocoma cf.dentata and Asteroidea Fromia milleporella continued
Organisms Thermal Limit
C 100 not responding
Salinity Limit psu
100 not responding
Upper Lower
Decrease Hourly
Hourly repeat
Daily Hourly
Hourly
Ophiocoma cf. dentata
nE: 10 TE1: 26.8
TE2: 40.5 nC: 10
TC1: 25.3 TC2:26.0
nE: 7 SE1: 36
SE2: 13 nC: 3
SC1: 36 SC2: 35
Fromia milleporella
nE: 7 TE1: 26.3
TE2: 35.8 nC: 3
TC1: 25.6 TC2: 26.2
nE: 10 TE1: 26.0
TE2: 35.0 nC: 10
TC1: 25.0 TC2: 25.9
nE: 10 TE1: 27.0
TE2: 33.0 nC: 4
TC1: 25.0 TC2: 25.5
Note: TE1: temperature in experiment tank when experiment started
TE2: temperature in experiment tank when all of the organisms not responding anymore
SE1: salinity in experiment tank when experiment started SE2: salinity in experiment tank when all of the organisms were not
responding anymore nE: number of organisms being used in experiment
TC1: temperature in control tank when experiment started TC2: temperature in control tank when the experiment finished when all of
the organisms in experiment tank were not responding anymore SC1: salinity in control tank when experiment started
SC2: salinity in control tank when the experiment finished when all of the organisms in experiment tank were not responding anymore
nC: number of organisms being used as control
4.1.2.1. Upper thermal limit hourly increase 4.1.2.1.1. Ophiomastix annulosa red ophiuroid
Red ophiuroid Ophiomastix annulosa during hourly increase temperature had an upper thermal limit 100 mortality or not responding of 36.5
C and 37.0 C on the repetition; the starting temperature was 26.0
C Table 3 and 4, Figure 13 and 15. The organisms reached their 50 mortality not response at more
than 36 C Figure 14 and 16. The organisms started to get weak at 35.0
C
ninth hour and 33.0 C sixth hour, repeat experiment Appendix 5 and 7.
Condition of the organisms at the end of both experiments: the arms were more or less straight and loose and could not turn. All organisms were already at the
bottom of the experimental tank. Table 3. Data of upper thermal limit experiment hourly increase of Ophiomastix
annulosa red ophiuroid. Experiment was done at 05:00-16:00 on April 19
th
2011.
Time Experiment
temperature C
Control temperature
C Number of
not response organisms
experiment n=10
Number of not response
organisms control
n=8
05:00 26.0
25.0 06:00
26.9 25.0
07:00 27.8
25.0 08:00
29.0 25.5
09:00 30.0
26.0 10:00
31.0 26.0
11:00 32.0
25.5 12:00
32.9 25.8
13:00 33.9
25.5 14:00
34.9 25.8
15:00 35.9
25.9 5
16:00 36.5
25.9 5