Ophiarachna incrassata green Ophiuroids

4.1.2.4.2. Ophiocoma cf.dentata black ophiuroids

Black ophiuroid Ophiocoma cf.dentata during hourly decrease salinity had a lower salinity limit 100 mortality or not responding of 13; the starting salinity was 36 Table 14, Figure 36. The organisms reached their 50 mortality not response at less than 16 Figure 37. Condition of the organisms on the sixth hour, some organisms‟ arms started to become stiff and curled 30. On the seventh hour, the organisms showed slow movement 29. On the twelfth hours, the organisms became stiffer but could still turn 24. On the fifteenth hour, all of the organisms became stiff and curled but could still turn 21. On sixteenth hour, the organisms could still turn but very slowly 20. On nineteenth hour, the first organism could not respond 17 Appendix 21. At the end of experiment the organisms showed weakness, stiffness, and none of the organisms could turn. The arms of the organisms were all stiff and curled. All organisms were already at the bottom of the experimental tank. The temperature in experiment and control tank was more or less stable during experiment Figure 38. Table 14. Data of lower salinity limit experiment hourly decrease of Ophiocoma cf.dentata Black Opiuroids. Experiment was done at 05:00-04:00 the next day on November 10 th -11 th 2011. Time Experiment salinity Control salinity Experiment temperature C Control temperature C Number of not response organisms experiment n=7 Number of not response organisms control n=3 05:00 36 36 26.4 25.6 06:00 35 36 26.4 25.6 07:00 34 35.5 26.1 25.8 08:00 33 35.5 26.0 25.9 09:00 32.5 35.5 26.0 25.9 10:00 31 35.5 26.0 25.9 11:00 30 35.5 26.0 25.9 12:00 29 35.5 26.0 25.9 13:00 28 35.5 26.0 25.9 14:00 27 35.5 26.0 26.0 15:00 26 35.5 26.0 26.0 16:00 25.5 35.5 26.1 26.0 17:00 24 35 26.0 26.0 18:00 23.5 35 26.1 26.0 19:00 22.5 35 26.0 25.8 20:00 21 35 26.1 25.8 Table 14. Data of lower salinity limit experiment hourly decrease of Ophiocoma cf.dentata Black Opiuroids. Experiment was done at 05:00-04:00 the next day on November 10 th -11 th 2011 continued. Time Experiment salinity Control salinity Experiment temperature C Control temperature C Number of not response organisms experiment n=7 Number of not response organisms control n=3 21:00 20 35 26.1 25.7 22:00 19.5 35 26.0 25.7 23:00 18.5 35 26.0 25.5 00:00 17 35 26.0 25.5 1 01:00 16 35 26.0 25.1 2 02:00 15 35 25.9 25.1 2 03:00 14 35 25.9 25.1 1 04:00 13 35 25.9 25.1 1 Figure 36. Lower salinity limit experiment hourly decrease of Ophiocoma cf.dentata Black Opiuroids. Experiment was done at 05:00-04:00 the next day on November 10 th -11 th 2011. 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 2 4 6 8 10 12 14 16 18 20 22 S al in ity p su Time hour Ophiocoma cf. dentata-November 10th-11th 2011 Experiment Control Figure 37. Number of not responding organisms from lower salinity limit experiment hourly decrease of Ophiocoma cf.dentata black opiuroids, accumulatively. Experiment was done at 05:00-04:00 the next day on November 10 th -11 th 2011. Figure 38. Temperature during lower salinity limit experiment hourly decrease of Ophiocoma cf.dentata black opiuroids. Experiment was done at 05:00-04:00 the next day on November 10 th -11 th 2011. 1 2 3 4 5 6 7 8 9 10 2 4 6 8 10 12 14 16 18 20 22 N u m b e r o f o rg an ism s Time hour Ophiocoma cf. dentata-November 10th-11th 2011 Experiment n=7 Control n=3 Salinity psu 13 36 30 28 24 22.5 20 14 13 14 15 16 17 34 32.5 26 16 18.5 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 2 4 6 8 10 12 14 16 18 20 22 Tem p e ratu re C Time hour Ophiocoma cf. dentata-November 10th-11th 2011 Experiment Control