Table 5. Data of upper thermal limit experiment hourly increase of Ophiarachna incrassata green ophiuroid. Experiment was done at 05:00-16:00 on
December 13
th
2010.
Time Experiment
temperature C
Control temperature
C Number of
not response organisms
experiment n=8
Number of not response
organisms control
n=8
05:00 25.9
24.2 06:00
26.1 24.8
07:00 28.0
25.0 08:00
29.2 25.1
09:00 30.0
25.2 10:00
30.5 25.3
11:00 31.6
25.4 12:00
32.9 25.5
13:00 34.0
25.5 14:00
35.2 25.5
15:00 36.2
25.5 1
16:00 37.2
25.5 7
Figure 17. Temperature rise during upper thermal limit experiment hourly increase of Ophiarachna incrassata green ophiuroid. Experiment
was done at 05:00-16:00 on December 13
th
2010.
24.0 25.0
26.0 27.0
28.0 29.0
30.0 31.0
32.0 33.0
34.0 35.0
36.0 37.0
38.0 39.0
40.0 41.0
42.0
1 2
3 4
5 6
7 8
9 10
11
Tem p
e ratu
re C
Time hour
Ophiarachna incrassata -December 13th 2010
Experiment Control
Figure 18. Number of not responding organisms from upper thermal limit experiment hourly increase of Ophiarachna incrassata green
ophiuroid, accumulatively. Experiment was done at 05:00-16:00 on December 13
th
2010.
4.1.2.1.3. Ophiocoma cf.dentata black Ophiuroid
Black ophiuroid Ophiocoma cf.dentata during hourly increase temperature
had an upper thermal limit 100 mortality or not responding of 40.5 C; the
starting temperature was 26.8 C Table 6, Figure 19. The organisms reached
their 50 mortality not response at more than 40 C Figure 20. The organisms
started to get slower movement at 32.3 C sixth hour Appendix 19. Condition
of the organisms at the end of experiment: the arms are more or less straight and loose and could not turn. All organisms were already at the bottom of the
experimental tank.
1 2
3 4
5 6
7 8
9 10
1 2
3 4
5 6
7 8
9 10 11
N u
m b
e r
o f n
o t
re sp
o n
d in
g
o rg
an ism
s
Time hour
Ophiarachna incrassata -December 13th 2010
Experiment n=8 Control n=8
Temp. C
37.2 25.9
35.2 34.0
31.6 37.2
36.2
28.0 30.0
Table 6. Data of upper thermal limit experiment hourly increase of Ophiocoma cf.dentata black ophiuroid. Experiment was done at 05:00-20:00 on
October 31
st
2011.
Time Experiment
temperature C
Control temperature
C Number of
not response organisms
experiment n=10
Number of not response
organisms control
n=10
05:00 26.8
25.3 06:00
26.8 25.6
07:00 27.1
25.9 08:00
28.5 26.0
09:00 29.9
26.0 10:00
31.2 26.0
11:00 32.3
26.0 12:00
33.4 26.0
13:00 34.2
26.0 14:00
35.2 26.0
15:00 36.0
26.1 16:00
36.8 26.0
17:00 38.0
26.0 18:00
38.8 26.0
19:00 40.0
26.0 2
20:00 40.5
26.0 8
Figure 19. Temperature rise during upper thermal limit experiment hourly increase of Ophiocoma cf.dentata black ophiuroid. Experiment was
done at 05:00-20:00 on October 31
st
2011.
Figure 20. Number of not responding organisms from upper thermal limit experiment hourly increase of Ophiocoma cf.dentata black
ophiuroid, accumulatively. Experiment was done at 05:00-20:00 on October 31
st
2011.
24.0 25.0
26.0 27.0
28.0 29.0
30.0 31.0
32.0 33.0
34.0 35.0
36.0 37.0
38.0 39.0
40.0 41.0
42.0
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Tem p
e ratu
re C
Time hour
Ophiocoma cf. dentata-October 31st 2011
Experiment Control
1 2
3 4
5 6
7 8
9 10
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 N
u m
b e
r o
f n o
t re
sp o
n d
in g
o rg
an ism
s
Time hour
Ophiocoma cf. dentata-October 31st 2011
Experiment n=10 Control n=10
Temp. C
40.5 26.8
38.0 36.0
40.5
40.0
27.1 29.9 34.2
32.3 40.0
4.1.2.1.4. Fromia milleporella red starfish
Red starfish Fromia milleporella during hourly increase temperature had an upper thermal limit 100 mortality or not responding of 35.8
C and 35.0 C;
the starting temperature was 26.3 C and 26.0
C Table 7 and 8, Figure 21 and 23. The organisms reached their 50 mortality not response at more than 34
C Figure 22 and 24. The organisms started to get weak at 33.0 C fifth hour
and 31.0 C seventh hour, repeat experiment Appendix 23 and 25. Condition
of the organisms at the end of both experiments: they lost their attachment capability and could not turn. All organisms were already at the bottom of the
experimental tank. Table 7. Data of upper thermal limit experiment hourly increase of Fromia
milleporella red starfish. Experiment was done at 05:00-14:00 on March 22
nd
2011.
Time Experiment
temperature C
Control temperature
C Number of
not response organisms
experiment n=7
Number of not response
organisms control
n=3
05:00 26.3
25.6 06:00
27.0 26.0
07:00 27.8
26.0 08:00
28.8 26.0
09:00 29.9
26.0 10:00
31.0 26.0
11:00 32.0
26.0 12:00
33.0 26.0
13:00 34.1
26.2 14:00
35.8 26.2
7
