Analisis jarak genetik berdasarkan marka ssrs dan morfologi serta analisis daya gabung untuk pembentukan hibrida jagung manis (Zea mays L. Var. Saccharata)

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(2)

Lampiran 1. Nilai matriks kemiripan 39 galur jagung manis

Mr4 /S C /B C 4-1 -1 B Mr4 /S C /B C 4-2 -1 B Mr4 /S C /B C 4-2 -2 B Mr4 /S C /B C 4-2 -3 B Mr4 /S C /B C 4-4 -1 B Mr4 /S C /B C 4-5 -1 B Mr4 /S C /B C 4-6 -1 B Mr4 /S C /B C 4-6 -2 B Mr4 /S C /B C 4-6 -3 B Mr4 /S C /B C 4-7 -1 B Mr4 /S C /B C 4-8 -1 B Mr4 /S C /B C 4-8 -2 B Mr4 /S C /B C 4-8 -3 B Mr4 /S C /B C 3-8 -2 B Mr1 1/ S C /B C 4-1 -1 B Mr1 1/ S C /B C 4-1 -2 B Mr1 1/ S C /B C 4-2 -1 B Mr1 1/ S C /B C 4-3 -1 B Mr1 1/ S C /B C 4-3 -2 B Mr1 1/ S C /B C 4-4 -1 B Mr1 1/ S C /B C 4-6 -1 B Mr1 1/ S C /B C 4-6 -2 B Mr1 1/ S C /B C 4-7 -2 B Mr1 1/ S C /B C 4-8 -1 B Mr1 2/ S C /B C 4-1 -1 B Mr1 2/ S C /B C 4-2 -1 B Mr1 2/ S C /B C 4-3 -1 B Mr1 2/ S C /B C 4-3 -2 B Mr1 2/ S C /B C 4-4 -1 B Mr1 2/ S C /B C 4-4 -2 B Mr1 2/ S C /B C 4-5 -1 B Mr1 2/ S C /B C 4-5 -2 B Mr1 2/ S C /B C 4-5 -3 B Mr1 2/ S C /B C 4-6 -1 B Mr1 2/ S C /B C 4-6 -2 B Mr1 2/ S C /B C 3-1 -1 B Mr1 2/ S C /B C 3-1 -2 B Mr1 2/ S C /B C 3-3 -1 B Mr1 2/ S C /B C 3-3 -2 B Mr4/SC/BC4-1-1B 1.00

Mr4/SC/BC4-2-1B 0.78 1.00 Mr4/SC/BC4-2-2B 0.82 0.78 1.00 Mr4/SC/BC4-2-3B 0.72 0.79 0.82 1.00 Mr4/SC/BC4-4-1B 0.54 0.54 0.60 0.57 1.00 Mr4/SC/BC4-5-1B 0.60 0.59 0.67 0.65 0.68 1.00 Mr4/SC/BC4-6-1B 0.76 0.72 0.76 0.69 0.58 0.67 1.00 Mr4/SC/BC4-6-2B 0.43 0.44 0.54 0.46 0.41 0.55 0.63 1.00 Mr4/SC/BC4-6-3B 0.40 0.41 0.45 0.59 0.47 0.63 0.50 0.47 1.00 Mr4/SC/BC4-7-1B 0.46 0.47 0.52 0.55 0.59 0.65 0.63 0.48 0.67 1.00 Mr4/SC/BC4-8-1B 0.43 0.48 0.48 0.57 0.56 0.62 0.50 0.41 0.57 0.65 1.00 Mr4/SC/BC4-8-2B 0.58 0.57 0.64 0.70 0.65 0.79 0.72 0.53 0.67 0.76 0.79 1.00 Mr4/SC/BC4-8-3B 0.56 0.56 0.63 0.78 0.64 0.78 0.71 0.57 0.65 0.62 0.64 0.83 1.00 Mr4/SC/BC3-8-2B 0.40 0.44 0.45 0.50 0.45 0.55 0.52 0.50 0.42 0.48 0.55 0.59 0.69 1.00 Mr11/SC/BC4-1-1B 0.33 0.34 0.33 0.40 0.35 0.39 0.43 0.44 0.34 0.42 0.31 0.38 0.45 0.48 1.00 Mr11/SC/BC4-1-2B 0.26 0.29 0.26 0.25 0.37 0.44 0.35 0.47 0.32 0.53 0.37 0.44 0.47 0.45 0.67 1.00

Mr11/SC/BC4-2-1B 0.38 0.39 0.38 0.45 0.45 0.45 0.40 0.36 0.42 0.54 0.50 0.43 0.46 0.45 0.64 0.56 1.00 Mr11/SC/BC4-3-1B 0.33 0.34 0.33 0.42 0.39 0.44 0.38 0.32 0.41 0.52 0.48 0.42 0.45 0.44 0.61 0.50 0.96 1.00 Mr11/SC/BC4-3-2B 0.28 0.29 0.28 0.36 0.39 0.39 0.35 0.28 0.32 0.42 0.43 0.38 0.40 0.39 0.68 0.50 0.87 0.88 1.00 Mr11/SC/BC4-4-1B 0.29 0.31 0.29 0.30 0.35 0.32 0.29 0.29 0.23 0.38 0.38 0.31 0.39 0.38 0.68 0.56 0.72 0.67 0.70 1.00 Mr11/SC/BC4-6-1B 0.27 0.29 0.27 0.30 0.29 0.29 0.28 0.27 0.28 0.36 0.33 0.29 0.34 0.34 0.66 0.65 0.63 0.60 0.67 0.84 1.00 Mr11/SC/BC4-6-2B 0.21 0.22 0.21 0.22 0.26 0.34 0.32 0.35 0.29 0.38 0.39 0.33 0.31 0.44 0.62 0.42 0.54 0.57 0.63 0.46 0.50 1.00 Mr11/SC/BC4-7-2B 0.22 0.23 0.22 0.28 0.36 0.41 0.43 0.36 0.43 0.48 0.41 0.39 0.39 0.55 0.59 0.53 0.42 0.43 0.45 0.44 0.39 0.72 1.00 Mr11/SC/BC4-8-1B 0.27 0.27 0.27 0.25 0.21 0.32 0.45 0.42 0.29 0.35 0.24 0.31 0.29 0.42 0.57 0.69 0.46 0.45 0.45 0.39 0.39 0.58 0.61 1.00 Mr12/SC/BC4-1-1B 0.19 0.21 0.19 0.21 0.32 0.29 0.27 0.30 0.28 0.35 0.25 0.28 0.26 0.30 0.48 0.47 0.50 0.48 0.48 0.35 0.31 0.44 0.38 0.48 1.00 Mr12/SC/BC4-2-1B 0.28 0.31 0.28 0.19 0.26 0.28 0.36 0.33 0.34 0.45 0.32 0.35 0.25 0.34 0.45 0.44 0.33 0.36 0.35 0.32 0.39 0.45 0.42 0.56 0.42 1.00 Mr12/SC/BC4-3-1B 0.32 0.38 0.32 0.26 0.34 0.30 0.47 0.40 0.41 0.54 0.39 0.38 0.31 0.35 0.48 0.47 0.48 0.45 0.43 0.35 0.37 0.45 0.48 0.52 0.50 0.76 1.00 Mr12/SC/BC4-3-2B 0.15 0.24 0.15 0.25 0.33 0.25 0.27 0.29 0.40 0.47 0.25 0.30 0.30 0.27 0.40 0.46 0.37 0.40 0.37 0.33 0.30 0.30 0.28 0.33 0.37 0.53 0.56 1.00 Mr12/SC/BC4-4-1B 0.35 0.41 0.35 0.30 0.47 0.38 0.38 0.34 0.33 0.50 0.42 0.45 0.39 0.42 0.41 0.43 0.42 0.41 0.36 0.37 0.35 0.32 0.36 0.40 0.47 0.72 0.64 0.56 1.00 Mr12/SC/BC4-4-2B 0.33 0.38 0.33 0.35 0.45 0.44 0.42 0.40 0.42 0.57 0.39 0.47 0.45 0.44 0.56 0.50 0.48 0.52 0.47 0.38 0.37 0.42 0.46 0.47 0.53 0.52 0.54 0.56 0.71 1.00 Mr12/SC/BC4-5-1B 0.26 0.31 0.26 0.29 0.41 0.37 0.30 0.26 0.45 0.56 0.37 0.40 0.38 0.33 0.41 0.47 0.58 0.61 0.50 0.43 0.43 0.31 0.32 0.40 0.42 0.54 0.52 0.79 0.65 0.63 1.00 Mr12/SC/BC4-5-2B 0.21 0.30 0.21 0.33 0.42 0.32 0.31 0.29 0.47 0.47 0.32 0.37 0.37 0.33 0.32 0.35 0.40 0.42 0.37 0.26 0.23 0.23 0.26 0.37 0.42 0.41 0.50 1.00 0.61 0.65 0.79 1.00 Mr12/SC/BC4-5-3B 0.32 0.28 0.32 0.40 0.41 0.35 0.31 0.50 0.52 0.48 0.35 0.39 0.45 0.42 0.40 0.44 0.45 0.42 0.33 0.37 0.33 0.22 0.29 0.36 0.32 0.38 0.41 0.67 0.50 0.46 0.61 0.71 1.00

Mr12/SC/BC4-6-1B 0.30 0.35 0.30 0.30 0.32 0.32 0.28 0.30 0.34 0.44 0.32 0.35 0.33 0.30 0.48 0.47 0.47 0.48 0.44 0.50 0.47 0.35 0.27 0.47 0.45 0.57 0.46 0.65 0.62 0.63 0.63 0.59 0.48 1.00 Mr12/SC/BC4-6-2B 0.27 0.32 0.27 0.26 0.33 0.33 0.35 0.31 0.39 0.45 0.38 0.36 0.39 0.34 0.41 0.42 0.42 0.45 0.45 0.40 0.39 0.36 0.32 0.40 0.42 0.54 0.58 0.53 0.53 0.55 0.62 0.63 0.50 0.57 1.00 Mr12/SC/BC3-1-1B 0.30 0.27 0.30 0.35 0.48 0.37 0.40 0.34 0.34 0.58 0.42 0.46 0.38 0.38 0.41 0.53 0.50 0.52 0.46 0.43 0.41 0.29 0.38 0.40 0.38 0.44 0.41 0.63 0.63 0.58 0.75 0.56 0.62 0.54 0.44 1.00 Mr12/SC/BC3-1-2B 0.23 0.21 0.23 0.32 0.34 0.34 0.30 0.31 0.41 0.48 0.39 0.38 0.33 0.27 0.34 0.41 0.46 0.48 0.43 0.36 0.28 0.33 0.39 0.42 0.50 0.31 0.36 0.47 0.41 0.54 0.48 0.56 0.52 0.56 0.52 0.64 1.00

Mr12/SC/BC3-3-1B 0.20 0.23 0.20 0.32 0.30 0.24 0.33 0.30 0.40 0.53 0.30 0.35 0.25 0.21 0.33 0.33 0.30 0.32 0.29 0.36 0.27 0.32 0.38 0.35 0.37 0.53 0.53 0.73 0.53 0.47 0.40 0.70 0.43 0.56 0.42 0.50 0.71 1.00 Mr12/SC/BC3-3-2B 0.28 0.35 0.28 0.33 0.48 0.31 0.30 0.24 0.36 0.52 0.42 0.40 0.35 0.28 0.37 0.38 0.48 0.48 0.46 0.44 0.38 0.32 0.35 0.29 0.42 0.48 0.50 1.00 0.67 0.61 0.75 0.71 0.59 0.67 0.50 0.74 0.59 0.69 1.00

Lamp

p

piran 1

Mr4/SCCC/BC4-1-1 Mr4/SCCC/BC4-2-1 Mr4/SCCC/BC4-2-2 Mr4/SCCC/BC4-2-3 Mr4/SCCC/BC4-4-1 Mr4/SCCC/BC4-5-1 Mr4/SCCC/BC4C-6-1 Mr4/SCC/BC4C/BC/BC4/BC4/BC/BC4/BC4/B/BC4/BC4/BC/BC4/BBC4BBC4BC4BCBCBC4BBBC4BCBCBBBC4BCBC4BCBCBC4BC4BC4BC4BC4BC4BCBBC4BBC4BBC4BCBBC4C4CCC4C4C444444-6--6-6-2-6--6--6-2-6-2-6--6-2-6-2-6-6-2-6--6-6--6--6--6--6-2-6-2-6-2-6-2-6-6--6-2-6-6-2-6-6 26666-66-26-26666--22

Mr4/SCCC/BC4/BC/BC4/B/B/B/BC4/BC4/BC4/B/BC/BC///BC4/BCBCBBCBCBBCBBC4BBBBBBBCBCBCBC4BC4BC4BCBCBCBCBCBCBCBBC4BCCCCC4CCC4CCCCCC-6-3-6-3-6--6-3-6-3-6--6-3-6-3-6-3-6-3-6--6-3-6--6--6--6-3-6-3666-36-366666 36-36 36-366-3666-33333

Mr4/SCCC/BC4BCBC4CC4C-7-1-7-777

Mr4/SCCC/BC4BC4CCC4C4CC4C4C4C4C4C4CCC4CCCCCCCC4C4C4CC4C4CC4C4C4C4C4C4C4C4CCC4C4C444-84444444-8--8-8-8--8-1-8-88888888888888888

Mr4/SCC/BC4CBC4BC4CC4C4CCC4CCC4C4C4C4CC4C4C4CC4CC4CC4C4C4CCCC4CC4CC4C4C4444444444444444444-8-8-8--8---8-8--8--8-8-2-8-888888888888888888888888

Mr4/SCC/BC4CBC4BC4CCCC4C4C4CCC4C4CC4C4CCCCC4CCC4-8-3C4C4C4CC4C4C4C4C4CCC4C4C4C4444444444444444444444---8---8--8 -Mr4/SCC/BC3CBCBBC3BC3BCBCBCBC3BCBCCCCCCCCCC3C3C3C3-CC3C3C3C3C3CC3C3CCCCCC3CC3CC3C3C3333333---8-8-2-8-8----88888888 Mr11/SSC/BCS BCBBCBCBCBCBCBCBBBCBBCBCBBCBBCBCBCBCBCBCBCBCBCBCBCBCBCBCBCBBBBCCCCCCCC4CCCCCCCCCCCCCCCCCC44-44-44444444444444444444444444-4-4-4-1-44444444-4-444444-444 Mr11/SSSC/BCBCBCBCBB4-1-444444 Mr11/SSC/BCSC/BBCBCBCBCBBCBCBCBBCBCBCBCBBCBBCBCBCBBCBCBCBCCC4CCCCCCCCCCCCCCCC44444444444444444444444444-24-4-2-4 Mr11/SSC/BCS BCBBBCBCBBBBCBBCBCBCBBBCBCBCBBBCBCBBBBBC4CCCCCC44-4-444-4444444-444-4-34444-4-34-3-4-4-44-4-4-344- -Mr11/SSC/BCS BBBCBCBBCBBCBBBBCBCBBCBCBCBCBBBBBBBCBCBCCCCCCCCCCCCCCCCCCCCCCCC4444444444444-4444444444-3-44 Mr11/SSC/BCS BCBCBCBBCBCBCBCBCBCBCBCBBCBCBBBCBCBCBBCBCCC4-4-CCCCCCCCCCCCCCCCCC44-4-4444-4444-444444444-44-4444-444-4444-4 -Mr11/SSC/BCS BBCBBBCBCBCBCBCBCBCBCBCBBCBCCCCCCCCCCCC4CCCCCCCCCCCCCCCCCCCCC444444444444444444444-4-4444-4-6-4444-4444444-4-44444

-Mr11/SSC/BCS BCBCBCBCBBBCBCBBCBBBCBBBBBCBCBBCBCBBCBBCBCBCBBCBCBBCBBBBBBCBBBBBCBCCCCCCCCCCCCCCCCCCC44444444444444444444-4-6-4-4-64-64-4-444444444-4

Mr11/SSC/BCS BCBBBCBCBCBBCBBBCBBCBCBCBCBBBCBCBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC44444444444444444444444444-7-4444-4444444444

Mr11/SSC/BCS BCBCBBBCBCBCBCBBCBCBCBCBCBCBCBCBCBCBCBCBBCBCBCBCBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC4444444444444444444444-4-444444-8-44

Mr12/SSC/BCS BCBBBCBBBBBCBBBCBBCBCBCBCBCBCBCBCBBCBCBCCCCCCCCCCCCCCCCCCCCCC4444444444444444444444444444-4-444-4-1-4-44-4-444444-444

Mr12/SSC/BCSC/BCBBBBBBCBCBBCBCBBBBBCBBBCBBCBCBCBCBCBBCBBCBCCCCCCCCCCCCCCCCCCCCCCCC44444444444444444444444-44-2444-2-4

Mr12/SSC/BCS BBCBBBBCBBBBCBBBBCBCBCBBCBBCBCBCBBCCCCCCCCCCCC4444444444-444-3-4-34-444---3 Mr12/SSC/BCS BBCBCBCBCBCBCBCBCBCBBCBBBCBBCBBBCBCBCBCBCBCBCBBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC444444444444444444444444-4-3-444444444444444

Mr12/SSC/BCSC/BCBCBBCBCBCBCBCBCBBCBCBCBBBCBCCCCCCCCCCCCCCCCCCCCCCCCCCC44444-444444444444444444444444-4-4-44-44444444-4-444444

Mr12/SSC/BCS BBCBCBCBCBCBCBBCBBBCBCBBCBCBCBBBBCBCBBCBCBCBCBBBCCCCCCCCCCCCCCCCCCC4-4-CCCCCCCCCCCCCCCCCCCC44444444444444444-44444444-4444444444

Mr12/SSC/BCS BBCBBBCBBBBBBBBBBBBCBCBCBCBCBCBBBCBBBCBCBCCCCCCCCCCCC44444-444-444444444444-44444-4-5-4-4-4-444-44-44444-4- -Mr12/SSC/BCS BCBCBBCBBBCBBBCBCBCBBCBBBCBBBBCBBCBBCCCCCCCCCCCCCCCCCCCCCCC44-44444444444444444444-5-4-44444444444 Mr12/SSC/BCS BCBCBBCBBBBCBCBBCC4-CC4-4-5-444-- -Mr12/SSC/BCSC/BBCBCBBBCBCBCBBCBCBCBCBBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC4-444444444444444-4-6-44-444444444444-444 Mr12/SSC/BCS BBBBBCBCBCBCBCBCBBBCBCBCBCBCBCBBBBBCBBBCBCBCBBCBBBCBCBCBCBCBCBCBC4-6-BCBCBCBCBCBBBCBCBCBBBCBCBBCBBCBCBBCBCCCCCCCCCCCCCCCCC4444444444444444444-44444444444444 Mr12/SSC/BCS BBCBBCBCBCBCBCBCBCBCBCBCBCBCBCBCBCBCBBCBCBBBCBCBCBCBCBCBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC33333333333333333333333333-1-3-33333333333

Mr12/SSC/BCS BCBCBBBBBBBBCBCBBBCBCBCBBCBBBBBBBCBBBCBCBCBCBCBCBCBCBCBCBCBBCBBBBCBCBBCBBBCCCCCCCCCCCCCCCCCCCCCCCCC33333333333333-3333333333333-33333-3-1-3-3333-33-33-3-3333333-3 Mr12/SS SC/BC3-3-Mr12/SS

(3)

SC/BC3-3-113

Lampiran 2 Matriks kemiripan 20 galur jagung manis berdasarkan marka SSRs

M

r4/SC

/BC

4-2

-1B

M

r4/SC

/BC

4-2

-2B

M

r4/SC

/BC

4-2

-3B

M

r4/SC

/BC

4-4

-1B

M

r4/SC

/BC

4-5

-1B

M

r4/SC

/BC

4-6

-1B

M

r4/SC

/BC

4-6

-2B

M

r4/SC

/BC

4-7

-1B

M

r4/SC

/BC

4-8

-1B

M

r4/SC

/BC

4-8

-2B

M

r4/SC

/BC

4-8

-3B

Mr

11/SC

/BC

4-

1-1B

M

r11/SC

/BC

4-3

-1B

M

r12/SC

/BC

4-1

-1B

M

r12/SC

/BC

4-5

-1B

M

r12/SC

/BC

4-5

-2B

M

r12/SC

/BC

4-5

-3B

M

r12/SC

/BC

4-6

-1B

M

r12/SC

/BC

4-6

-2B

M

r12/SC

/BC

3-1

-1B

Mr4/SC/BC4-2-1B

1.00 Mr4/SC/BC4-2-2B

0.78

1.00 Mr4/SC/BC4-2-3B

0.79

0.82

1.00 Mr4/SC/BC4-4-1B

0.54

0.60

0.57

1.00 Mr4/SC/BC4-5-1B

0.59

0.67

0.65

0.68

1.00 Mr4/SC/BC4-6-1B

0.72

0.76

0.69

0.58

0.67

1.00 Mr4/SC/BC4-6-2B

0.44

0.54

0.46

0.41

0.55

0.63

1.00 Mr4/SC/BC4-7-1B

0.47

0.52

0.55

0.59

0.65

0.63

0.48

1.00 Mr4/SC/BC4-8-1B

0.48

0.57

0.56

0.62

0.50

0.41

0.65

1.00 Mr4/SC/BC4-8-2B

0.57

0.64

0.70

0.65

0.79

0.72

0.53

0.76

0.79

1.00 Mr4/SC/BC4-8-3B

0.56

0.63

0.78

0.64

0.78

0.71

0.57

0.62

0.64

0.83

1.00 Mr11/SC/BC4-1-1B

0.34

0.33

0.40

0.35

0.39

0.43

0.44

0.42

0.31

0.38

0.45

1.00 Mr11/SC/BC4-3-1B

0.34

0.33

0.42

0.39

0.44

0.38

0.32

0.52

0.48

0.42

0.45

0.61

1.00 Mr12/SC/BC4-1-1B

0.21

0.19

0.21

0.32

0.29

0.27

0.30

0.35

0.25

0.28

0.26

0.48

1.00 Mr12/SC/BC4-5-1B

0.31

0.26

0.29

0.41

0.37

0.30

0.26

0.56

0.37

0.40

0.38

0.41

0.61

0.42

1.00 Mr12/SC/BC4-5-2B

0.30

0.21

0.33

0.42

0.32

0.31

0.29

0.47

0.32

0.37

0.32

0.42

0.79

1.00 Mr12/SC/BC4-5-3B

0.28

0.32

0.40

0.41

0.35

0.31

0.50

0.48

0.35

0.39

0.45

0.40

0.42

0.32

0.61

0.71

1.00 Mr12/SC/BC4-6-1B

0.35

0.30

0.32

0.28

0.30

0.44

0.32

0.35

0.33

0.48

0.45

0.63

0.59

0.48

1.00 Mr12/SC/BC4-6-2B

0.32

0.27

0.26

0.33

0.35

0.31

0.45

0.38

0.36

0.39

0.41

0.45

0.42

0.62

0.63

0.50

0.57

1.00 Mr12/SC/BC3-1-1B

0.27

0.30

0.35

0.48

0.37

0.40

0.34

0.58

0.42

0.46

0.38

0.41

0.52

0.38

0.75

0.56

0.62

0.54

0.44

1.00

Lampi

i

iran

Mr4/SC/

/

BC4-2

Mr4/SC/

/

BC4-2

Mr4/SC/

/

BC

B

BC

B

BC

C

C4

C

4 4-

4 -2

-

2 Mr4/SC/

/

BC

C

C4

C

4 4-4

4 Mr4/SC/

/

BC

C

C4

C

4 4-

4 -

-5

Mr4/SC/

/

BC

C

4 -

-6

Mr4/SC/

/

BC

C

C4

C

4 4-

4 -

-6

Mr4/SC/

/

BC

C

4 4-

4 -

-7

Mr4/SC/

/

BC

C

C4

C

4 4-

4 -8

Mr4/SC/

/

BC

C

4 4-

4 -

--8

Mr4/SC/

/

BC

C

C4

C

4 4-

4 -

8 Mr11/SC

C/B

C

B

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

4 -Mr11/SC

C/B

C

B

BC4

B

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4-

4 Mr12/SC

C/B

C

B

BC

B

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

-Mr12/SC

C/B

C

B

BC4

B

C

C4

C

C4

C

C4

C

-Mr12/SC

C/B

C

B

BC

B

C

C4

C

C4

C

C4

C

-Mr12/SC

C/B

C

B

BC4

B

C

C4

C

C4

C

C4

C

C4

C

C4

C

-Mr12/SC

C/B

C

B

BC

B

C

C4

C

C4

C

C4

C

C4

C

-Mr12/SC

C

C/BC4-Mr12/SC

C

(4)

C/BC3-114

Lampiran 3 Matriks kemiripan 20 galur jagung manis berdasarkan data morfologi

M

R

4/S

C/BC4

-4

-1

B

M

R

4/S

C/BC4

-2

-1

B

M

R

4/S

C/BC4

-7

-1

B

M

R

4/S

C/BC4

-5

-1

B

M

R

4/S

C/BC4

-2

B

M

R

4/S

C/BC4

-8

-2

B

M

R

4/S

C/BC4

-6

-1

B

M

R

4/S

C/BC4

-2

-3

B

M

R

4/S

C/BC4

-8

-1

B

M

R

4/S

C/BC4

-8

-3

B

M

R

4/S

C/BC4

-6

-2

B

M

R

11/S

C/BC4

-3-1

M

R

11/S

C/BC4

-1-1

M

R

12/S

C/

BC4

-6-1

M

R

12/S

C/BC4

-1-1

M

R

12/S

C/BC4

-5-2

M

R

12/S

C/BC4

-6-2

M

R

12/S

C/BC4

-5-3

M

R

12/S

C/BC3

-1-1

M

R

12/S

C/BC4

-5-1

MR4/SC/BC4-4-1B

1.00 MR4/SC/BC4-2-1B

0.75

1.00 MR4/SC/BC4-7-1B

0.75

1.00 MR4/SC/BC4-5-1B

0.56

0.75

1.00 MR4/SC/BC4-2-2B

0.56

0.40

1.00 MR4/SC/BC4-8-2B

0.75

0.56

1.00 MR4/SC/BC4-6-1B

0.75

0.56

1.00 MR4/SC/BC4-2-3B

0.56

0.40

0.75

1.00 MR4/SC/BC4-8-1B

0.56

0.75

0.40

0.56

0.40

1.00 MR4/SC/BC4-8-3B

0.56

0.40

1.00

0.56

0.40

1.00 MR4/SC/BC4-6-2B

0.75

1.00

0.75

0.56

0.75

0.56

1.00 MR11/SC/BC4-3-1

0.40

0.27

0.56

0.75

0.27

0.56

0.40

1.00 MR11/SC/BC4-1-1

0.56

0.40

0.56

0.75

0.56

0.40

0.56

1.00 MR12/SC/BC4-6-1

0.56

0.40

0.56

0.27

0.40

0.56

0.27

0.40

0.56

1.00 MR12/SC/BC4-1-1

0.27

0.40

0.27

0.40

0.56

0.40

0.27

0.56

1.00 MR12/SC/BC4-5-2

0.56

0.40

0.75

0.56

0.40

0.56

0.75

0.56

0.27

0.56

1.00 MR12/SC/BC4-6-2

0.75

0.56

0.40

0.56

0.75

0.40

0.56

0.75

0.40

1.00 MR12/SC/BC4-5-3

0.56

0.75

0.40

0.60

0.56

0.40

1.00

0.40

0.56

0.27

0.56

0.40

1.00 MR12/SC/BC3-1-1

0.40

0.56

0.75

0.40

0.27

0.56

0.40

0.56

0.27

0.40

0.27

0.56

1.00 MR12/SC/BC4-5-1

0.56

0.75

0.40

0.56

0.40

1.00

0.40

0.56

0.27

0.56

0.40

1.00

0.56

1.00

Lampi

i

iran

MR4/SC

C

/BC4-4

MR4/SC

C

/BC4-2

MR4/SC

C

/BC

BC

B

BC

B

BC

B

BC

B

BC

B

BC

B

BC

B

BC

B

BC

B

BC

B

BC

B

BC

C

4 4-

4 -

-7

-

7 MR4/SC

C

/BC

BC

C

4 -5

MR4/SC

C

/BC

BC

C

C4

C

4 -2

MR4/SC

C

/BC

BC

C

4 4-8

MR4/SC

C

/BC

BC

B

BC

B

C

4 4-6

MR4/SC

C

/BC

BC

C

4 -2

MR4/SC

C

/BC

BC

C

C4

C

4 -8

MR4/SC

C

/BC

BC

C

4 4-8

MR4/SC

C

/BC

BC

C

C4

C

4 -6

MR11/SC

C/B

C

/B

/

B

C

C4

C

C4

C

MR11/SC

C/B

/B

B

BC

B

C

C4

C

C4

C

MR12/SC

C/B

C

/B

B

C

C4

C

C4

C

C4

C

C4

C

MR12/SC

C/B

C

/B

B

BC

B

C4

C

C4

C

C4

C

MR12/SC

C/B

C

/B

B

C

C4

C

MR12/SC

C

C/B

/B

B

BC4

B

C

C4

C

MR12/SC

C/B

C

/B

B

C

C4

C

MR12/SC

C/B

C

/B

B

BC3

B

C

(5)

115 Lampiran 4 Matriks kemiripan 20 galur jagung manis berdasarkan marka SSRs dan data

morfologi

M

r4/S

C/BC4

-2

-1

B

M

r4/S

C/BC4

-2

B

M

r4/S

C/BC4

-2

-3

B

M

r4/S

C/BC4

-4

-1

B

M

r4/S

C/BC4

-5

-1

B

M

r4/S

C/BC4

-6

-1

B

M

r4/S

C/BC4

-6

-2

B

M

r4/S

C/BC4

-7

-1

B

M

r4/S

C/BC4

-8

-1

B

M

r4/S

C/BC4

-8

-2

B

M

r4/S

C/BC4

-8

-3

B

M

r11/S

C/BC4

-1-1

M

r11/S

C/BC4

-3-1

M

r12/S

C/BC4

-1-1

M

r12/S

C/BC4

-5-1

M

r12/S

C/BC4

-5-2

M

r12/S

C/BC4

-5-3

M

r12/S

C/BC4

-6-1

M

r12/S

C/BC4

-6-2

M

r12/S

C/BC3

-1-1

Mr4/SC/BC4-2-1B

1.00 Mr4/SC/BC4-2-2B

0.78

1.00 Mr4/SC/BC4-2-3B

0.79

0.82

1.00 Mr4/SC/BC4-4-1B

0.54

0.60

0.57

1.00 Mr4/SC/BC4-5-1B

0.59

0.67

0.65

0.68

1.00 Mr4/SC/BC4-6-1B

0.72

0.76

0.69

0.58

0.67

1.00 Mr4/SC/BC4-6-2B

0.44

0.54

0.46

0.41

0.55

0.63

1.00 Mr4/SC/BC4-7-1B

0.47

0.52

0.55

0.59

0.65

0.63

0.48

1.00 Mr4/SC/BC4-8-1B

0.48

0.57

0.56

0.62

0.50

0.41

0.65

1.00 Mr4/SC/BC4-8-2B

0.57

0.64

0.70

0.65

0.79

0.72

0.53

0.76

0.79

1.00 Mr4/SC/BC4-8-3B

0.56

0.63

0.78

0.64

0.78

0.71

0.57

0.62

0.64

0.83

1.00 Mr11/SC/BC4-1-1

0.34

0.33

0.40

0.35

0.39

0.43

0.44

0.42

0.31

0.38

0.45

1.00 Mr11/SC/BC4-3-1

0.34

0.33

0.42

0.39

0.44

0.38

0.32

0.52

0.48

0.42

0.45

0.61

1.00 Mr12/SC/BC4-1-1

0.21

0.19

0.21

0.32

0.29

0.27

0.30

0.35

0.25

0.28

0.26

0.48

1.00 Mr12/SC/BC4-5-1

0.31

0.26

0.29

0.41

0.37

0.30

0.26

0.56

0.37

0.40

0.38

0.41

0.61

0.42

1.00 Mr12/SC/BC4-5-2

0.30

0.21

0.33

0.42

0.32

0.31

0.29

0.47

0.32

0.37

0.32

0.42

0.79

1.00 Mr12/SC/BC4-5-3

0.28

0.32

0.40

0.41

0.35

0.31

0.50

0.48

0.35

0.39

0.45

0.40

0.42

0.32

0.61

0.71

1.00 Mr12/SC/BC4-6-1

0.35

0.30

0.32

0.28

0.30

0.44

0.32

0.35

0.33

0.48

0.45

0.63

0.59

0.48

1.00 Mr12/SC/BC4-6-2

0.32

0.27

0.26

0.33

0.35

0.31

0.45

0.38

0.36

0.39

0.41

0.45

0.42

0.62

0.63

0.50

0.57

1.00 Mr12/SC/BC3-1-1

0.27

0.30

0.35

0.48

0.37

0.40

0.34

0.58

0.42

0.46

0.38

0.41

0.52

0.38

0.75

0.56

0.62

0.54

0.44

1.00

Lampi

i

iran

Mr4/SC/

B

BC

C

4 4-2

Mr4/SC/

BC

B

BC

B

BC

B

BC

B

BC

B

BC

B

BC

B

BC

B

BC

B

BC

C

4 -

2 Mr4/SC/

BC

B

C

C4

C

4 4-

4 -

-2

Mr4/SC/

BC

B

C

C4

C

4 4-

4 -

-4

Mr4/SC/

BC

B

C

4 -

-5

Mr4/SC/

BC

B

C

C4

C

4 -

6 Mr4/SC/

BC

B

C

4

44

4 -

-6

Mr4/SC/

BC

B

C

4 -

-7

Mr4/SC/

BC4

B

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

4 -

-8

Mr4/SC/

BC

B

C

C4

C

4 4-

4 -

-8

Mr4/SC/

BC

B

C

4 -

8 Mr11/SC

C/B

C

B

BC

B

C

C4

C

C4

C

C4

C

C4

C

C4

C

-Mr11/SC

C/B

C

B

BC

B

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

4 -Mr12/SC

C/B

C

B

BC4

B

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

C4

C

4 -Mr12/SC

C/B

C

B

C

C4

C

C4

C

C4

C

C4

C

C4

C

-Mr12/SC

C/B

C

B

C

C4

C

C4

C

C4

C

C4

C

C4

C

-Mr12/SC

C/B

C

B

C

C4

C

C4

C

C4

C

C4

C

4 -Mr12/SC

C/B

C

B

BC4

B

C4

C

C4

C

C4

C

C4

C

C4

C

C4

4 -Mr12/SC

C/B

C

B

BC

B

C

C4

C

C4

C

C4

C

-Mr12/SC

C/BC3

C

BC

B

BC

B

BC

B

BC

BC3

BC

BC3

BC

BC3

C

(6)

-ABSTRACT

R.NENI IRIANY M.

Genetic Distance Analysis based on SSRs and Morphological

Marker and Combining Ability Analysis for sweet corn development

(Zea mays L. var. saccharata) hybrid. Under supervised

SRIANI SUJIPRIHATI

(in

memoriam),

MUHAMAD SYUKUR, JAJAH KOSWARA

(in memoriam)

,

and

MUHAMAD YUNUS.

The objective of the research were to grouping sweet corn lines resistant to

downy mildew, high specific and general combining ability and high heterosis to

select parental line to breed single cross hybrid of sweet corn.

The first research was done to study genetic variability of 39 sweet corn lines

based on SSR and morphological marker to select good parental lines of hybrid

sweet corn. There were high genetic variability among genetic material, showed by

genetic distance value about 0.18 to 0.78, indicates wide genetic variability of sweet

corn. The results shows that among 20 lines having genetic similarity between 0.25

–

0.82 or genetic distance from 0.18 to 0.75; based on morphological trait 0.27

–

1.00. Correlation value between similarity matrix based on SSR and morphology was high

on 0.88 of goodness of fit criteria.

The aim of second research was to study general combining ability of sweet

corn lines for yield and resistant to downy mildew for hybrid varieties improvement.

Six of 45 lines having high fresh ear yield per plot and best general combining

ability, such as Mr4/SC/BC4-2-1B, Mr4/SC/BC4-6-1B, Mr11/SC/BC4-2-1B,

Mr11/SC/BC4-4-1B, Mr12/SC/BC4-6-1B, and Mr12/SC/BC3-3-1B.

All of testing lines have resistance to downy mildew with severity about 0.0 to

7.1%. The lines having high GCA value would be good combiner to other lines to

develop high yielding hybrid sweet corn and resistant to downy mildew.

The third research was done to study general and specific combining ability of

sweet corn lines and higher heterosis of downy mildew resistance supporting the

hybrid corn development. The combination of A x D lines (Mr12/SC/BC4-6-1B x

Mr11/SC/BC4-2-1B) were medium genetic distance and having highest GCA,

heterosis and heterobeltiosis for fresh ear yield per plot character. The D x C

combination (Mr11/SC/BC4-2-1B x Mr4/SC/BC4-2-1B) have wide genetic distance

and high fresh ear yield per plot, heterosis and heterobeltiosis, but have negative

SCA value. The combination of E x C (Mr12/SC/BC3-3-1B x Mr4/SC/BC4-2-1B)

have a wide genetic distance but low productivity.

The best hybrid is not always obtained from parents with the high genetic

distance, but also can be obtained from the low or medium genetic distance as well.