Kesimpulan Saran KESIMPULAN DAN SARAN

BAB 5 KESIMPULAN DAN SARAN

5.1 Kesimpulan

1. Ekstrak etanol jinten hitam dosis 125mgkgBB, 250 mgkgBB dan 500 mg kgBB mampu mempengaruhi jumlah total leukosit dan mempengaruhi persentase limfosit 2. Ekstrak etanol jinten hitam dosis 125mgkgBB, 250 mgkgBB dan 500 mg kgBB tidak menunjukkan pengaruh terhadap persentase monosit dan kadar IL- 1β 3. Ekstrak etanol jinten hitam dosis 125mgkgBB, 250 mgkgBB dan 500 mg kgBB mampu mempengaruhi jumlah total leukosit dan persentase limfosit

5.2 Saran

Dilakukan uji lanjutan untuk melihat lama penurunan jumlah total leukosit setelah pemberian ekstrak etanol jinten hitam. 40 UIN Syarif Hidayatullah Jakarta DAFTAR PUSTAKA Abbas, Abul K, dan Andrew H. Lichtman, 2006. Basic Immunology 2nd Edition. Elsevier – Health Sciences Div Abdulelah, H.A.A. dan Zainal-Abidin, B. A. H. 2007. In Vivo Anti-malarial Tests of Nigella sativa Black Seed Different Extracts . Science Publications. 2 2: 46- 50 Adamu, harami, Ekanem, Bulama. 2010. Identification of Essential Oil Components from Nigella sativa Seed by Gas Chromatography-Mass Spectroscopy . Abubakar Tafawa Balewa University. 9 10: 966-967. Aggarwal, Bharat B., Kunnumakkara, Ajaikumar B. 2009. Molecular Targets and Therapeutic Uses of Spices Modern Uses for Ancient Medicine . World Scientific. Hal. 258-260. Anington LR. 1972. Introductory Laboratory Animal Science. The Breeding, Care and Managenment of Experinmenta1 Animals . New York: The Interstate Printers Publishers Inc. Arifiani AA. 2012. 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Immunomodulatory and therapeutic properties of the Nigella sativa L. seed . Int. J. Immunopharmacol: 1749– 1770. Santoso singgih. 2008. Panduan Lengkap Menguasai SPSS 16. PT. Elex Media Komputindo. Jakarta: 239-247, 315-319 Shoieb AM, Elgayyar M, Dudrick PS, Bell JL, Tithof PK. In Vitro Inhibition Of Growth And Induction Of Apoptosis In Cancer Cell Lines By Thymoquinone . Int J Oncol 2003; 22: 107–13. UIN Syarif Hidayatullah Jakarta Singh, gurdip. dkk. 2005. Chemical constituents and antimicrobial and antioxidant potentials of essential oil and acetone extract of Nigella sativa seeds. Chemistry Department, DDU Gorakhpur University. 85:2297–2306. Sloane E. Anatomi dan Fisiologi. Jakarta: EGC Penerbit Buku Kedokteran. Sutrisno, BB. 1986. Analisis Jamu Edisi I. Fakultas Farmasi Universitas Pancasila: Jakarta. Hal. 6-9. Suhatri, Aldi Y. 2010. Aktifitas Ekstrak Etanol Biji Jintan Hitam Nigella sativa Linn. Terhadap Titer Antibodi Dan Jumlah Sel Leukosit Pada Mencit Putih Jantan . Fakultas Farmasi Universitas Andalas. Sukowati S, 2010. Masalah Vektor Demam Berdarah Dengue DBD dan Pengedaliannya di Indonesia. Buletin Jendela Epidemiologi. Vol 2 Sumartini S, Zuas O, Julismardiany R, Susilawati E. Aplikasi Elisa Kit Untuk Mendeteksi Adanya Daging Babi Dalam Makanan . Pusat Penelitian Kimia: Tangerang Swamy S.M.K. 1998. Cytotoxic and Immunopotentiating Effects of Ethanolic Extract of Nigella sativa L. Seeds. Department of Pharmacology, Faculty of Medicine, National University of Singapore. 70 2000 1–7. Tahir K, Bakeet D. 2006. A Plea for Urgent Clinical Evaluation of its Volatile Oil. Department of Pharmacology, College of Pharmacy, King Saud University Riyadh Saudi Arabia: 2 – 3 Tousson E, El-Moghazy E, El-Atrsh E. 2011.The possible effect of diets containing Nigella sativa and Thymus vulgaris on blood parameters and some organs structure in rabbit. Toxicology and Industrial Health Underwood, JCE.1996. General and Systematic Pathology Second Edition. Penerjemah: Sarjadi. Jakarta: Penerbit Buku Kedokteran EGC. 190 Virella G. 1997. Introduction to Medical Immunology Fourth Edition. New York: Marcel Dekker UIN Syarif Hidayatullah Jakarta Vieira K. 2011. Improving Abnormal Results. University Of Florida College Of Medicine Yeheya M. 2009. A Review Therapeutic Role of Phrophetic Medicine Habbat El Baraka Nigella sativa L.. Departement of Pharmacy. 7 9: 1203-1208. UIN Syarif Hidayatullah Jakarta Lampiran 1 . Surat Keterangan Hewan Uji UIN Syarif Hidayatullah Jakarta Lampiran 2 . Alat dan Bahan yang digunakan Hemasitometer Tabung Tamo Mikroskop Setrifus UIN Syarif Hidayatullah Jakarta Differesial Leukosit Counter Larutan Turk Larutan Giemsa UIN Syarif Hidayatullah Jakarta Lampiran 3. Alur Penelitian Jinten hitam berpotensi sebagai anti inflamasi, anti oksidan, antitumor dan beberapa penelitian menunjukan bahwa jinten hitam berkhasiat sebagai imunomodulator Dilakukan peneletian untuk membandingkan dan mengetahui efektifitas ekstrak etanol Jinten hitam terhadap jumlah total leukosit mencit dan jumlah IL- 1β pada mencit yang diberikan LPS Serbuk Jinten hitam Ekstrak Etanol Aklitimasi Hewan Uji Pemberian ekstrak etanol selama 14 hari berturut -turut Hari ke – 5 dua jam setelah ekstrak etanol, diberikan LPS 20 μgkgBB mencit. Enam jam kemudian diambil darah melalui retro-orbital plexus Pengambilan darah melalui retro-orbital plexus pada hari ke 7, hari ke 14 dan hari ke 21 Pemberian ekstrak etanol selama 5 hari berturut -turut Perhitungan Total Leukosit, Limfosit dan Monosit Penentuan kadar IL- 1β menggunakan ELISA UIN Syarif Hidayatullah Jakarta Lampiran 4. Pembuatan Larutan Uji

A. Pembuatan Larutan Uji Ekstrak Etanol Untuk Uji Total Leukosit

1. Dosis Rendah 2.5 mg20 gr BB atau 125 mgkg BB VaO Mencit ∶ Dosis � mg kg BB � × Berat Badan kg Konsentrasi 0.5 ml ∶ 125 mg kg ×0.02kg Konsentrasi Konsetrasi : 5 mgml VaO Total : VaO x Jumlah Mencit x Lama Pemberian : 0.5 ml x 8 x 14 : 56 ml x 2 : 112 ml Jumlah ekstrak yang ditimbang : VaO Total x Konsentrasi : 112 ml X 5 mgml : 560 mg 2. Dosis Sedang 5 mg 20 grBB atau 250 mgkgBB VaO Mencit ∶ Dosis � mg kg BB � × Berat Badan kg Konsentrasi 0.5 ml ∶ 250 mg kg ×0.02kg Konsentrasi Konsetrasi : 10 mgml VaO Total : VaO x Jumlah Mencit x Lama Pemberian : 0.5 ml x 8 x 14 : 56 ml x 2 : 112 ml Jumlah ekstrak yang ditimbang : VaO Total x Konsentrasi : 112 ml X 10 mgml : 1120 mg UIN Syarif Hidayatullah Jakarta 3. Dosis Tinggi 10 mg20 grBB atau 500 mgkgBB VaO Mencit ∶ Dosis � mg kg BB � × Berat Badan kg Konsentrasi 0.5 ml ∶ 500 mg kg ×0.02kg Konsentrasi Konsetrasi : 20 mgml VaO Total : VaO x Jumlah Mencit x Lama Pemberian : 0.5 ml x 8 x 14 : 56 ml x 2 : 112 ml Jumlah ekstrak yang ditimbang : VaO Total x Konsentrasi : 112 ml X 20 mgml : 2240 mg

B. Pembuatan Larutan Uji Ekstrak Etanol Untuk Uji Interleukin 1 Beta

1. Dosis Rendah 2.5 mg20 gr BB atau 125 mgkg BB VaO Mencit ∶ Dosis � mg kg BB � × Berat Badan kg Konsentrasi 0.5 ml ∶ 125 mg kg ×0.02kg Konsentrasi Konsetrasi : 5 mgml VaO Total : VaO x Jumlah Mencit x Lama Pemberian : 0.5 ml x 5 x 14 : 12.5 x 2 : 25 ml Jumlah ekstrak yang ditimbang : VaO Total x Konsentrasi :25 ml X 5 mgml :125 mg 2. Dosis Sedang 5 mg 20 grBB atau 250 mgkgBB VaO Mencit ∶ Dosis � mg kg BB � × Berat Badan kg Konsentrasi 0.5 ml ∶ 250 mg kg ×0.02kg Konsentrasi UIN Syarif Hidayatullah Jakarta Konsetrasi : 10 mgml VaO Total : VaO x Jumlah Mencit x Lama Pemberian : 0.5 ml x 5 x 14 : 12.5ml x 2 : 25 ml Jumlah ekstrak yang ditimbang : VaO Total x Konsentrasi : 25 ml X 10 mgml : 250 mg 3. Dosis Tinggi 10 mg20 grBB atau 500 mgkgBB VaO Mencit ∶ Dosis � mg kg BB � × Berat Badan kg Konsentrasi 0.5 ml ∶ 500 mg kg ×0.02kg Konsentrasi Konsetrasi : 20 mgml VaO Total : VaO x Jumlah Mencit x Lama Pemberian : 0.5 ml x 5 x 14 : 12.5 :25 ml Jumlah ekstrak yang ditimbang : VaO Total x Konsentrasi : 25 ml X 20 mgml : 500 mg Lampiran 5. Kegiatan Penelitian Apusan Darah Fiksasi UIN Syarif Hidayatullah Jakarta Lampiran 6 . Gambar Pemeriksaan Total Leukosit, Monosit dan Limfosit Monosit Limfosit Total Leukosit UIN Syarif Hidayatullah Jakarta Lampiran 7. Jumlah Total Leukosit, Presendase Monosit Dan Limfosit Hari 7

1. Jumlah Total Leukosit Pengambilan Hari 7

Kelompok No Total Lekosit Rata - Rata Total Leukosit mm 3 I II Kontrol 1 112 91 101.5 5075 2 59 86 72.5 3625 5 64 83 73.5 3675 7 37 110 73.5 3675 8 93 117 105 5250 Rata - Rata 85.2 4260.0 Etanol Dosis Rendah 1 83 85 84 4200 2 167 131 149 7450 3 78 99 88.5 4425 4 185 217 201 10050 7 107 100 103.5 5175 Rata - Rata 125.2 6260.0 Etanol Dosis Sedang 1 162 172 167 8350 2 158 182 170 8500 4 134 140 137 6850 7 144 140 142 7100 8 148 130 139 6950 Rata - Rata 151.0 7550.0 Etanol Dosis Tinggi 1 211 178 194.5 9725 2 285 278 281.5 14075 3 192 150 171 8550 4 241 203 222 11100 6 250 234 242 12100 Rata - Rata 222.2 11110.0 UIN Syarif Hidayatullah Jakarta

2. Persentase Monosit dan Limfosit Hari 7

Kelompok No Differensial Lekosit Jumlah Differensial Leukosit Monosit Limfosit Eusonofil Basofil Monosit Limfosit Eusonofil Basofil Kontrol 1 8 86 1 5 406 4364.5 50.75 253.75 2 9 85 1 6 326.25 3081.25 36.25 217.5 5 8 89 2 2 294 3270.75 73.5 73.5 7 4 89 2 5 147 3270.75 73.5 183.75 8 1 95 2 2 52.5 4987.5 105 105 Rata - Rata 6 89 2 4 245.2 3795.0 67.8 166.7 Etanol Dosis Rendah 1 1 99 42 4158 2 5 94 1 372.5 7003 74.5 3 6 94 265.5 4159.5 4 11 88 1 1105.5 8844 100.5 7 12 88 621 4554 Rata - Rata 7 93 481.3 5743.7 20.1 14.9 Etanol Dosis Sedang 1 4 96 334 8016 2 8 90 2 680 7650 170 4 4 95 1 1 274 6507.5 68.5 68.5 7 3 96 1 213 6816 71 8 5 95 347.5 6602.5 Rata – Rata 5 94 1 369.7 7118.4

13.7 61.9