BAB VI KESIMPULAN DAN SARAN
VI.1. Kesimpulan
Dari hasil penelitian pada 100 serum penderita Demam Dengue dan Demam Berdarah Dengue yang dikumpulkan dari beberapa Rumah Sakit di Kota Medan
yaitu Rumah Sakit Pirngadi, Rumah Sakit H. Adam Malik, dan Rumah Sakit Herna, Setelah diekstraksi dan di RT-PCR tidak ada dijumpai negatif serum yang
mengandung virus serotipe 3 DEN 3.
VI.2 Saran
Berdasarkan kesimpulan yang telah diuraikan, maka dapat diusulkan saran- saran sebagai berikut:
1. Perlu dilakukan penelitian untuk mendeteksi virus Dengue lainnya seperti DEN 1,
DEN 2, dan DEN 4. 2.
Perlu penentuan virus Dengue dalam manajemen pasien Demam DengueDemam Beradarah Dengue untuk mempermudah pencapaian program pemberantasan
penyakit Deman DengueDeman Berdarah Dengue.
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DAFTAR PUSTAKA
Chow, V. T, Chan, Y. C, Yong, R. et al, 1998. Monitoring of Dengue Viruses in
Field Cought Aedes Aegypti and Aedes Albopictus by A Type-Specific PCR and Cycle Squencing, American Journal of Tropical Medicine and Hygiene
585: 578-586.
Darmowandowo,W. 2006. Kuliah Infeksi Virus Dengue. Naskah lengkap disajikan dalam Continuing Education Ilmu Kesehatan Anak XXXVI. Fakultas
Kedokteran Unair. Surabaya. 29–30 Juli 2006. Hadinegoro, S et al, 2006. Tata Laksana Demam Berdarah Dengue di Indonesia.
Departemen Kesehatan Republik Indonesia. Jakarta. Hal. 2 – 19. Halstead, S. B. Deen, J. 1969. The Future of Dengue Vaccines. Lancet 360, 1243-
1245. Handrawan N, 2007. Cara Mudah Mengalahkan Demam Berdarah. Kompas.
Jakarta. Hal. 17-18. Hariadhi, S, Soegijanto, S. 2004. Pola Distribusi Serotipe Virus Dengue pada
Beberapa Daerah Endemik di Jawa Timur dengan Kondisi Geografis Berbeda, Hal. 11-19.
Harris, E; Robert, T. G; Smith, L; Selle, J et al. 1998. Typing of Dengue Viruses in Clinical Specimen and Mosquitoes by Single-Tube Multiplex Reverse
Transcriptase PCR. Journal of Clinical Microbiology. Sept. 1998. p. 2634 – 9.
Kristina,Isminah,Wulandari L. 2004 Kasus Demam Berdarah Dengue DBD di Indonesia. Wahono TD Ed. Buletin Harian. Tim Penanggulangan DBD
Departemen Kesehatan RI, 2004. Kusumawati, Lia. 2005 Teori Sequential Infection dari Halstead online.
http:www.library.usu.ac.iddownloadfkmikrobiologipdf. Massi, M. N; Sabran, A. 2006. Teknik Identifikasi Serotipe Virus Dengue DEN 1 -
4 dengan Uji Reverse Transcriptase Polymerase Chain Reaction RT-PCR. Fakultas Kedokteran Universitas Hasanuddin.
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Miagostovich, M.P.et al, 2001. Genetic characterization of Dengue virus type 3 isolate in the state of Rio de Janeiro. Brazillian journal of medical and
Biological Research 2002 35 : 869-872. Nisalak A, Endy TP, Nimmannitya S, Kalayanarooy S, Thisayakorn U, Scott RM,
Burke DS, Hoke CH, InnisBL, Vaughn DW, 2003, Serotype-spesific Dengue Virus Circulation and Dengue Disease in Bangkok, Thailand from
1973 to 1999. Am J Trop Med Hyg; 68 2 : 191-202.
Setya, B. 2007. Profil Serologis Infeksi Primer dan Sekunder Virus Dengue dari Berbagai Daerah di Jawa Timur. Post Graduate Airlangga University E-
mail: http:librarylib.unair.ac.id; libraryunair.ac.id. Singh Kamaljit, et al, 2006. A prospective Clinical study on the use of reverse
transcription polymerase chain reaction for the early diagnosis of Dengue Fever, The Journal of Molecular Diagnostics. Vol 6 No 5.
Siregar, A. F, 2004. Epidemiologi dan Pemberantasan Demam Berdarah Dengue di Indonesia. Fakultas Kesehatan Masyarakat Universitas Sumatera Utara.
Sopian, T,
2006. Aplikasi
Teknologi PCR
mendeteksi Flu
Burung http:64.203.71.11kompas-cetak, 17 Mei 2008.
Suroso, 1999. Epidemiological situation of Dengue Haemorrhagic Fever and its Control Indonesia. Proceeding InternationalSeminar on Dengue Fever
Dengue Haemorrhagic. TDC- Airlangga University, Surabaya.p. 11-4. Sudjadi, 2008. Bioteknologi Kesehatan. Penerbit Kanisius. Yogyakarta. Hal. 94 –
99 dan 131 - 43. World Health Organization Regional Office for South-East Asia, 1997.
Management of Dengue Epidemic. Report of A Technical Meeting, SEARO, New Delhi, 28 – 30 November 1996.
Yulfi. H, 2006. Persistency of Transsovarian Dengue Virus in Aedes Aegypti on line http : library.usu.ac.iddown loadfkpdf.
Yuwono, D. 2001. Hubungan Antara Genotup Virus Dengue dengan Severity Penyakit Berdasarkan Respon Imun Selularnya. Pusat Penelitian Penyakit
Menular, Badan Penelitian dan Pengembangan Kesehatan, Departemen Kesehatan RI. Jakarta.
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Lampiran 1. Contoh Formulir Data Pasien Formulir Pemeriksaan Kasus DDDBD
I. Informasi Umum
Nama Lengkap : Umur :
Jenis Kelamin : pria wanita Alamat :
Pekerjaan :
II. Hasil Laboratorium
1. Laboratorium Rutin
- Leukosit 5.000 selml
3
ya tidak -
Trombosit 100.000 sel ya tidak -
Hemokonsentrasi 20 ya tidak 2.
Laboratorium Penunjang -
Rapid Test IgM ya tidak IgG ya tidak
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Lampiran 2. Deteksi dan penentuan virus Dengue serotipe 3 dari spesimen klinik di Rumah Sakit Kota Medan dengan menggunakan Metode
RT-PCR
SAMPEL RUMAH SAKIT UMUR
JNS KEL DEN 3 + DEN 3 -
1 2
3 4
5 6
7 8
9
10 11
12 13
14 15
16 17
18 19
20 21
22 23
24 25
26 27
28 29
30 31
32 33
34 35
36 37
RS PR RS PR
RS PR RS PR
RS PR RS PR
RS HAM RS HAM
RS H RS H
RS PR RS HAM
RS HAM RS HAM
RS HAM RS HAM
RS HAM RS PR
RS PR RS HAM
RS HAM RS HAM
RS HAM RS HAM
RS PR RS PR
RS PR RS PR
RS PR RS PR
RS H RS H
RS H RS H
RS PR RS H
RS PR 6
11 9
8 11
18 58
18 14
16 17
20 20
15 12
38
1,5 24
29 45
11
9 13
5 9
21 44
23 22
39 17
9 18
19 21
25 28
LK PR
LK PR
PR LK
LK LK
LK
PR LK
LK PR
LK LK
PR LK
PR PR
PR
LK LK
PR PR
PR PR
LK PR
PR LK
LK PR
PR PR
LK LK
LK -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
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38 39
40 41
42 43
44 45
46 47
48 49
50 51
52 53
54 55
56 57
58 59
60 61
62 63
64 65
66 67
68 69
70 71
72 73
74 75
76 77
78 79
RS PR RS PR
RS PR RS PR
RS PR RS H
RS H RS PR
RS PR RS PR
RS PR RS H
RS HAM RS H
RS PR RS H
RS H RS PR
RS PR RS PR
RS HAM RS PR
RS PR RS H
RS H RS H
RS PR RS PR
RS PR RS H
RS HAM RS H
RS HAM RS HAM
RS HAM RS HAM
RS HAM RS HAM
RS HAM RS HAM
RS H RS HAM
32 25
19 29
31 41
21 49
21 21
30 41
3 14
15 40
9 16
19 30
9 30
41 9
21 21
22 11
44 12
44 16
6 10
8 4
6 5
43 25
16
7 LK
LK LK
LK LK
PR PR
LK PR
PR PR
LK PR
LK PR
LK PR
PR LK
PR PR
LK LK
PR PR
PR PR
LK PR
PR PR
LK PR
PR LK
LK PR
LK LK
LK LK
LK -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
-
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80 81
82 83
84 85
86 87
88 89
90 91
92 93
94 95
96 97
98 99
100 RS HAM
RS HAM RS HAM
RS H RS PR
RS H RS H
RS H RS PR
RS HAM RS HAM
RS HAM RS HAM
RS HAM RS HAM
RS HAM RS HAM
RS HAM RS HAM
RS HAM RS HAM
8 11
6 44
82 29
24 44
39 10
21 10
11 12
16 17
20 23
17 30
11 LK
LK PR
LK LK
LK
PR LK
PR PR
PR
LK LK
PR PR
PR
LK LK
LK
PR LK
- -
- -
- -
- -
- -
- -
- -
- -
- -
- -
-
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Lampiran 3. Rencana Kegiatan Penelitian
September Oktober
Nopember Desember
Januari No
Kegiatan I II III IV I II III IV I II III IV I II III IV I II III IV
1 Pengumpulan
Serum penderita.
2 Ekstraksi.
3 RT-PCR
dengan menggunakan
PrimersDEN 3.
4 Elektroforesis
dan Visualisasi.
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