LAMPIRAN B. Data dan analisis statistik kerusakan histologi tubulus proksimal ginjal mencit
Persentase jumlah kerusakan histologi tubulus proksimal ginjal mencit
Mus musculus L. setelah pemberian kombinasi ekstrak air biji pepaya
Carica papaya L. dan Testosteron Undekanoat TU , X±SD Lama perlakuan
Minggu Kontrol
Perlakuan 30.26±3.90
34.78±4.26 6
30.96±2.92 47.86±5.57
12 29.27±3.66
42.19±6.60 18
34.68±2.90 40.72±4.03
24 35.73±2.23
38.73±7.17 Hasil uji statistik histologi tubulus proksimal ginjal mencit
a. Kelompok kontrol
Tests of Normality
.303 5
.151 .787
5 .064
.185 5
.200 .930
5 .596
.212 5
.200 .890
5 .357
.214 5
.200 .928
5 .586
.210 5
.200 .962
5 .819
K_KONTROL K0
K1 K2
K3 K4
G_NEKROSIS Statistic
df Sig.
Statistic df
Sig. Kolmogorov-Smirnov
a
Shapiro-Wilk
This is a lower bound of the true significance. .
Lilliefors Significance Correction a.
Test of Homogeneity of Variance
.354 4
20 .838
.131 4
20 .969
.131 4
13.904 .969
.318 4
20 .863
Based on Mean Based on Median
Based on Median and with adjusted df
Based on trimmed mean G_NEKROSIS
Levene Statistic
df1 df2
Sig.
Universitas Sumatera Utara
Oneway
Post Hoc Tests
b. Kelompok perlakuan
ANOV A G_NEK ROSIS
162.509 4
40.627 4.016
.015 202.328
20 10.116
364.836 24
Between Groups W ithin Groups
Total Sum of
Squares df
Mean S quare F
Sig.
Multiple Com pari sons
Dependent Variable: G_NE KROSIS Bonferroni
-.70400 2.01161
1.000 -7. 0474
5.6394 .98200
2.01161 1.000
-5. 3614 7.3254
-4. 42400 2.01161
.398 -10.7674
1.9194 -5. 47600
2.01161 .131
-11.8194 .8674
.70400 2.01161
1.000 -5. 6394
7.0474 1.68600
2.01161 1.000
-4. 6574 8.0294
-3. 72000 2.01161
.793 -10.0634
2.6234 -4. 77200
2.01161 .278
-11.1154 1.5714
-.98200 2.01161
1.000 -7. 3254
5.3614 -1. 68600
2.01161 1.000
-8. 0294 4.6574
-5. 40600 2.01161
.142 -11.7494
.9374 -6. 45800
2.01161 .044
-12.8014 -.1146
4.42400 2.01161
.398 -1. 9194
10.7674 3.72000
2.01161 .793
-2. 6234 10.0634
5.40600 2.01161
.142 -.9374
11.7494 -1. 05200
2.01161 1.000
-7. 3954 5.2914
5.47600 2.01161
.131 -.8674
11.8194 4.77200
2.01161 .278
-1. 5714 11.1154
6.45800 2.01161
.044 .1146
12.8014 1.05200
2.01161 1.000
-5. 2914 7.3954
J K_K ONTROL K1
K2 K3
K4 K0
K2 K3
K4 K0
K1 K3
K4 K0
K1 K2
K4 K0
K1 K2
K3 I K_K ONTROL
K0
K1
K2
K3
K4 Mean
Difference I-J
St d. E rror Sig.
Lower Bound Upper Bound
95 Confidenc e Interval
The mean differenc e is significant at the .05 level. .
Tests of Normality
.421 5
.004 .656
5 .003
.210 5
.200 .950
5 .739
.324 5
.094 .785
5 .060
.212 5
.200 .916
5 .503
.210 5
.200 .924
5 .557
K_PERLAKUAN P0
P1 P2
P3 P4
G_NEKROSIS Statistic
df Sig.
Statistic df
Sig. Kolmogorov-Smirnov
a
Shapiro-Wilk
This is a lower bound of the true s ignificance. .
Lilliefors Significance Correction a.
Universitas Sumatera Utara
Friedman Test
Wilcoxon Signed Ranks Test
K0P0 T-Test
Test of Homogeneity of Variance
1.526 4
20 .233
.653 4
20 .632
.653 4
15.294 .634
1.492 4
20 .242
Based on Mean Based on Median
Based on Median and with adjusted df
Based on trimmed mean G_NEKROSIS
Levene Statistic
df1 df2
Sig.
Ra nks
1.40 4.40
3.40 3.20
2.60 P0
P1 P2
P3 P4
Mean Rank
Test Statistics
a
5 9.760
4 .045
N Chi-Square
df As ymp. Sig.
Friedman Test a.
Test Statistics
c
-2.023
a
-1.483
a
-2.023
a
-1.214
a
-1.483
b
-1.483
b
-1.753
b
-.405
b
-.674
b
-.135
b
.043 .138
.043 .225
.138 .138
.080 .686
.500 .893
Z As ymp. Sig. 2-tailed
P1 - P0 P2 - P0
P3 - P0 P4 - P0
P2 - P1 P3 - P1
P4 - P1 P3 - P2
P4 - P2 P4 - P3
Based on negative ranks . a.
Based on positive ranks. b.
Wilcoxon Signed Ranks Test c.
Group Statistics
5 1.4780
.06221 .02782
5 1.5380
.06058 .02709
K_KONTROL KONTROL
PERLAKUAN K0P0
N Mean
Std. Deviation Std. Error
Mean
Independent Samples Test
.000 1.000
-1.545 8
.161 -.06000
.03883 -.14955
.02955 -1.545
7.994 .161
-.06000 .03883
-.14956 .02956
Equal variances as sumed
Equal variances not ass umed
K0P0 F
Sig. Levenes Test for
Equality of Variances t
df Sig. 2-tailed
Mean Difference
Std. Error Difference
Lower Upper
95 Confidence Interval of the
Difference t-test for Equality of Means
Universitas Sumatera Utara
K1P1 T-Test
K2P2 T-Test
K3P3 T-Test
Group Statistics
5 1.4900
.04062 .01817
5 1.6800
.05099 .02280
K_KONTROL KONTROL
PERLAKUAN K1P1
N Mean
Std. Deviation Std. Error
Mean
Independent Samples Test
.330 .582
-6.517 8
.000 -.19000
.02915 -.25723
-.12277 -6.517
7.619 .000
-.19000 .02915
-.25782 -.12218
Equal variances as sumed
Equal variances not ass umed
K1P1 F
Sig. Levenes Test for
Equality of Variances t
df Sig. 2-tailed
Mean Difference
Std. Error Difference
Lower Upper
95 Confidence Interval of the
Difference t-test for Equality of Means
Group Statistics
5 1.4640
.05814 .02600
5 1.6220
.06723 .03007
K_KONTROL KONTROL
PERLAKUAN K2P2
N Mean
Std. Deviation Std. Error
Mean
Independent Samples Test
.886 .374
-3.975 8
.004 -.15800
.03975 -.24966
-.06634 -3.975
7.837 .004
-.15800 .03975
-.25000 -.06600
Equal variances as sumed
Equal variances not ass umed
K2P2 F
Sig. Levenes Test for
Equality of Variances t
df Sig. 2-tailed
Mean Difference
Std. Error Difference
Lower Upper
95 Confidence Interval of the
Difference t-test for Equality of Means
Group Statistics
5 1.5380
.03421 .01530
5 1.6080
.04147 .01855
K_KONTROL KONTROL
PERLAKUAN K3P3
N Mean
Std. Deviation Std. Error
Mean
Independent Samples Test
.126 .732
-2.912 8
.020 -.07000
.02404 -.12544
-.01456 -2.912
7.720 .020
-.07000 .02404
-.12579 -.01421
Equal variances as sumed
Equal variances not ass umed
K3P3 F
Sig. Levenes Test for
Equality of Variances t
df Sig. 2-tailed
Mean Difference
Std. Error Difference
Lower Upper
95 Confidence Interval of the
Difference t-test for Equality of Means
Universitas Sumatera Utara
K4P4 T-Test
Group Statistics
5 1.5520
.02683 .01200
5 1.5800
.08485 .03795
K_KONTROL KONTROL
PERLAKUAN K4P4
N Mean
Std. Deviation Std. Error
Mean
Independent Samples Test
7.165 .028
-.704 8
.502 -.02800
.03980 -.11978
.06378 -.704
4.792 .514
-.02800 .03980
-.13166 .07566
Equal variances as sumed
Equal variances not ass umed
K4P4 F
Sig. Levenes Test for
Equality of Variances t
df Sig. 2-tailed
Mean Difference
Std. Error Difference
Lower Upper
95 Confidence Interval of the
Difference t-test for Equality of Means
Universitas Sumatera Utara
LAMPIRAN C. Alat dan bahan penelitian
Dissecting set Bak bedah
Tempat fiksasi organ ginjal Oven
Mikrotom rotary
Carica papaya L. Ekstrak air biji pepaya
Testosteron Undekanoat Kertas Saring TU
Tempat pemeliharaan hewan percobaan
Hewan percobaan Mus musculus L.
Universitas Sumatera Utara
LAMPIRAN D. Prosedur pembuatan ekstrak air biji pepaya Carica papaya L.
Ilyas, 2001
dibersihkandicuci dikeringkan dalam incubator dengan suhu 50
diblender hingga halus C ± 3 hari
dimasukkan ke dalam bejanapanci ditambahkan air
direbus di atas hotplate hingga mendidih dengan suhu 90 disaring dengan kertas saring
C
direbus kembali hingga tidak dapat dipergunakan lagi
dipanaskan hingga diperoleh rendaimen rendaimen dilarutkan kembali dengan 500 ml aquabidestilata
sesuai dengan kebutuhan penelitian Biji pepaya
Carica papaya L.
Residu
Hasil
Universitas Sumatera Utara
LAMPIRAN E. Prosedur uji skrining fitokomia biji pepaya Carica papaya L.
Harborne, 1987 a.
Uji flavonoid
dikeringkan dihaluskan
dimasukkan ke dalam erlenmeyer yang berisi 100 ml methanol dipanaskan hingga ¼ volume awal kemudian disaring
dimasukkan ke dalam 4 tabung reaksi tabung I ditetesi FeCl
3
, tabung II ditetesi MgHCl, tabung III ditetesi H
2
SO
4
diamati perubahan warna yang terjadi dan dicatat hasilnya p, tabung IV ditetesi NaOH 10, masing-masing
sebanyak 3-5 tetes
b. Uji Alkaloid
