Pembahasan Umum Toxicity of heavy metal’s lead (Pb) and its effects on oxygen consumption and hematological response of juvenile tiger grouper (Epinephelus fuscoguttatus)

Setelah uji 96 jam Konsentrasi Pb ppm Persentase Mortalitas Ikan pada jam ke- Total 78 84 90 96 0 0 0 0 0 0 20 0 0 0 0 0 40 5 5 0 0 10 80 0 0 5 0 5 160 0 0 0 0 0 Lampiran 2. Hasil analisis probit LC 50 -24 jam Selang Kepercayaan Probabilitas Selang kepercayaan 95 untuk perlakuan Estimasi Batas bawah Batas atas PROBIT .010 5.412 -32.373 26.250 .020 15.376 -17.961 34.309 .030 21.698 -8.906 39.513 .040 26.454 -2.154 43.486 .050 30.323 3.293 46.763 .060 33.615 7.892 49.589 .070 36.502 11.894 52.099 .080 39.087 15.448 54.374 .090 41.438 18.655 56.469 .100 43.602 21.584 58.420 .150 52.562 33.431 66.780 .200 59.683 42.455 73.816 .250 65.792 49.861 80.187 .300 71.279 56.219 86.202 .350 76.362 61.854 92.032 .400 81.186 66.977 97.789 .450 85.854 71.737 103.554 .500 90.447 76.252 109.398 .550 95.040 80.617 115.392 .600 99.708 84.919 121.616 .650 104.532 89.246 128.168 .700 109.615 93.695 135.184 .750 115.102 98.392 142.860 .800 121.211 103.518 151.511 .850 128.332 109.384 161.704 .900 137.292 116.636 174.659 .910 139.456 118.371 177.805 .920 141.807 120.248 181.229 .930 144.392 122.305 185.001 .940 147.279 124.595 189.223 .950 150.571 127.196 194.047 .960 154.440 130.240 199.727 .970 159.196 133.967 206.726 .980 165.518 138.898 216.053 .990 175.482 146.626 230.797 Lampiran 3. Hasil analisis probit LC 50 -48 jam Selang Kepercayaan Probabilitas Selang kepercayaan 95 untuk perlakuan Estimasi Batas bawah Batas atas PROBIT .010 17.906 -24.278 35.301 .020 25.037 -11.526 40.645 .030 29.561 -3.515 44.115 .040 32.965 2.457 46.780 .050 35.733 7.272 48.990 .060 38.090 11.334 50.908 .070 40.156 14.863 52.623 .080 42.006 17.993 54.187 .090 43.688 20.812 55.638 .100 45.237 23.380 57.000 .150 51.649 33.681 62.971 .200 56.745 41.363 68.222 .250 61.117 47.486 73.193 .300 65.043 52.565 78.078 .350 68.682 56.907 82.968 .400 72.134 60.721 87.915 .450 75.474 64.162 92.950 .500 78.761 67.346 98.109 .550 82.049 70.365 103.432 .600 85.389 73.296 108.976 .650 88.841 76.212 114.822 .700 92.479 79.185 121.082 .750 96.406 82.304 127.926 .800 100.778 85.692 135.632 .850 105.874 89.557 144.700 .900 112.286 94.323 156.206 .910 113.835 95.462 158.997 .920 115.517 96.694 162.034 .930 117.367 98.044 165.379 .940 119.433 99.546 169.121 .950 121.790 101.251 173.395 .960 124.558 103.247 178.425 .970 127.962 105.690 184.619 .980 132.486 108.921 192.870 .990 139.617 113.983 205.903 Lampiran 4. Hasil analisis probit LC 50 -72 jam Selang Kepercayaan Probabilitas Selang kepercayaan 95 untuk perlakuan Estimasi Batas bawah Batas atas PROBIT .010 2.184 -36.908 20.731 .020 10.631 -23.352 27.256 .030 15.990 -14.832 31.478 .040 20.021 -8.479 34.710 .050 23.300 -3.356 37.383 .060 26.091 .968 39.695 .070 28.538 4.727 41.755 .080 30.729 8.062 43.629 .090 32.722 11.068 45.361 .100 34.557 13.809 46.981 .150 42.151 24.833 54.015 .200 48.187 33.111 60.087 .250 53.366 39.779 65.732 .300 58.016 45.381 71.187 .350 62.326 50.238 76.575 .400 66.415 54.569 81.967 .450 70.371 58.528 87.414 .500 74.264 62.235 92.964 .550 78.158 65.786 98.670 .600 82.114 69.263 104.599 .650 86.203 72.747 110.838 .700 90.513 76.320 117.510 .750 95.163 80.087 124.799 .800 100.341 84.197 133.001 .850 106.377 88.902 142.648 .900 113.972 94.724 154.883 .910 115.807 96.117 157.851 .920 117.799 97.626 161.081 .930 119.991 99.279 164.637 .940 122.438 101.120 168.615 .950 125.229 103.212 173.158 .960 128.508 105.662 178.505 .970 132.539 108.662 185.090 .980 137.898 112.634 193.859 .990 146.344 118.862 207.713 Lampiran 5. Output analisis probit LC 50 -96 jam Selang Kepercayaan Probabilitas Selang kepercayaan 95 untuk perlakuan Estimasi Batas bawah Batas atas PROBIT .010 -4.558 -44.336 14.328 .020 4.018 -30.636 20.915 .030 9.459 -22.014 25.165 .040 13.552 -15.578 28.412 .050 16.881 -10.381 31.091 .060 19.715 -5.990 33.403 .070 22.200 -2.168 35.460 .080 24.425 1.227 37.328 .090 26.448 4.291 39.050 .100 28.311 7.088 40.659 .150 36.022 18.377 47.612 .200 42.150 26.905 53.582 .250 47.408 33.807 59.119 .300 52.130 39.621 64.475 .350 56.505 44.664 69.782 .400 60.657 49.153 75.115 .450 64.674 53.246 80.524 .500 68.627 57.067 86.056 .550 72.580 60.716 91.758 .600 76.597 64.281 97.696 .650 80.749 67.844 103.955 .700 85.124 71.493 110.656 .750 89.846 75.335 117.984 .800 95.104 79.523 126.233 .850 101.232 84.315 135.940 .900 108.944 90.241 148.256 .910 110.806 91.659 151.244 .920 112.829 93.195 154.495 .930 115.054 94.878 158.075 .940 117.539 96.751 162.079 .950 120.373 98.881 166.654 .960 123.702 101.374 172.037 .970 127.795 104.428 178.666 .980 133.236 108.470 187.495 .990 141.812 114.810 201.443 Lampiran 6. Frekwensi pergerakan operkulum juvenil ikan kerapu macan selama uji akut Perlakuan Frekwensi 1 Frekwensi 2 Frekwensi 3 Rerata A Kontrol 85 88 93 89 B 20 ppm 87 91 95 91 C 40 ppm 93 96 98 96 D 80 ppm 105 110 107 107 E 160 ppm 134 129 136 133