Halimi Pasteran V. Villegas Blackburn K. Boyd

94 SCIENTIFIC SESSIONS September 9-12 | ------------------------------------------------------------------------ 091 D Poster Session Mycobacteria: Diagnostics and Susceptibility Testing Monday, 11:15 a.m.–1:15 p.m. . . . . . . . . . . . . . . . . . . . Halls A-C Presentations: D-732 Use of RT-PCR for the Detection of Mycobacterium tuberculosis Complex DNA

M. Rowlinson

1 , S. R. Crowe 1 , Y. Lee 1 , P. Fiorella 1 , R. Farah 1 , M. Pedrosa 1 , M. Salfinger 2 ; 1 Florida Dept. of Hlth., Jacksonville, FL, 2 Florida Dept. of Hlth., Tallahassee, FL. D-733 Comparative Evaluation of Conventional Methods and Nested Polymerase Chain Reaction in the Diagnosis of Extra- Pulmonary Tuberculosis

S. Sharma; Sgrdimsar, Amritsar, India. D-734

Comparative Evaluation of Advansure Mycobacteria Genoblot Assay Kit for the Identification of Nontuberculous Mycobacteria to the Species Level

H. Bae, G. Ryu, E. Lee; Green Cross Reference Lab., Yongin, Korea, Repub- lic of.

D-735 Rapid Species Identification of Mycobacterium chelonae and Mycobacterium abscessus Complex and Determination of Inducible Clarithromycin Resistance by High Resolution Melt Analysis HRM M. J. Tuohy, G. S. Hall, G. W. Procop, S. M. Harrington; Cleveland Clinic, Cleveland, OH. D-736 Evaluation of the TREK MIC MicroTiter Plate Method for Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis Complex Isolates C. Tanner 1 , P. Miles 1 , M. Rowlinson 1 , S. Crowe 1 , D. Willis 1 , M. Salfinger 2 ; 1 Bureau of Lab., Florida Dept. of Hlth., Jacksonville, FL, 2 Bureau of Lab., Florida Dept. of Hlth., Tallahassee, FL. D-737 Comparison of a Commercial MTB MIC Plate, Agar Propor- tion Method, and DNA Sequence Analysis for Detection of Resis- tance to Isoniazid, Rifampin, and Ethambutol in Mycobacterium tuberculosis Complex

D. Sikes, B. Metchock; CDC, Atlanta, GA. D-738

Drug Susceptibility Testing and Genotypes of Mycobacterium tuberculosis Isolated from Extra-Pulmonary Clinical Specimens of Bangladesh Withdrawn D-739 Conversion of CO to CO2 by CO Dehydrogenase as a Highly Selective Stable Isotope Breath Test Marker for Tuberculosis Diagnosis

S. Choi

1 , M. Maiga 2 , M. Maiga 2 , V. Atudorei 1 , Z. Sharp 1 , W. Bishai 2 , G. Timmins 1 ; 1 Univ. of New Mexico, Albuquerque, NM, 2 Johns Hopkins Hosp., Baltimore, MD. D-740 Mycobacterial IGRA Variations in Long-Term Treated Patients with TNF Blockers are not Associated with Tuberculosis Outcome I. Sauzullo 1 , R. Scrivo 1 , F. Mengoni 1 , R. Marocco 2 , G. Valesini 1 , V. Vullo 1 , C. M. Mastroianni 2 ; 1 Sapienza Univ., Rome, Italy, 2 Sapienza Univ., Latina, Italy. D-741 Diagnostic Usefulness of a T-Cell-Based Assay in Patients with Miliary Tuberculosis TB Y. Lee, K. Park, S. Kim, S. Park, S. Lee, S. Choi, Y. Kim, J. Woo, S. Kim; Asan Med. Ctr., Seoul, Korea, Republic of. D-742 Diagnosis of Latent Tuberculosis in Subjects with Discordant TSTIGRA Results I. Sauzullo 1 , A. Ermocida 1 , F. Mengoni 1 , R. Rossi 1 , M. Lichtner 2 , V. Vullo 1 ,

C. M. Mastroianni

2 ; 1 Sapienza Univ., Rome, Italy, 2 Sapienza Univ., Latina, Italy. D-743 Extrapulmonary Tuberculosis: Epidemiological, Molecular and Circulating Cytokines Markers J. Fortun, P. Martin-Davila, E. Gomez-Mampaso, A. Vallejo, I. Barbolla, P. Wikman, J. Ortíz, E. Navas, D. Gijon, I. Gomez-Garcia, A. Gonzalez, J. Rubi, E. Pallares, S. Moreno; Ramon Y Cajal Hosp., Madrid, Spain. D-744 Approach to Latent Tuberculosis Among Occupational Health Providers

C. VinnardE

1 , D. LinkinE 2 , A. Behrman 2 ; 1 Drexel Univ. Coll. of Med., Philadelphia, PA, 2 Univ. of Pennsylvania, Philadelphia, PA. ------------------------------------------------------------------------ 092 D Poster Session Phenotypic Susceptibility Testing Methods Monday, 11:15 a.m.–1:15 p.m. . . . . . . . . . . . . . . . . . . . Halls A-C Presentations: D-745 Evaluation of Two Etest MBL Strips for Detection of Metallo- Carbapenemases in Enterobacteriaceae

D. Halimi

1 , D. Girlich 2 , P. Nordmann 2 , S. Rivat 1 , G.Durand1, R.Martelin 1 , G. Zambardi 1 ; 1 bioMerieux, La Balme les Grottes, France, 2 Hosp. Bicetre, Paris, France. D-746 Screening of Epidemiologically Challenging Carbapenemase Producers Pseudomonas aeruginosa CP-PA by Agar Dilution AD, Vitek 2C and Phoenix Systems: Are Current Breakpoints Enough?

F. Pasteran

1 , C. Lucero 1 , M. M. Peres 2 , L. Errecalde 3 , O. Veliz 1 , M. Rapoport 1 , E. Tuduri 1 , A. Di Bella 4 , S. Kaufman 3 , D. Korbenfeld 2 , A. Corso 1 ; 1 Antimicrobianos, INEI-ANLIS, Buenos Aires, Argentina, 2 BD Diagnostics Systems, Buenos Aires, Argentina, 3 Hosp. Fernandez, Buenos Aires, Argentina, 4 Hosp. Posadas, Buenos Aires, Argentina. D-747 Unreliability of the New CLSI 2012 Breakpoints for Cefepime and Carbapenems to Detect KPCs

M. V. Villegas

1 , A. Correa 1 , E. De La Cadena 1 , J. J. Maya 1 , V. M. Blanco 1 , L. J. Rojas 1 , M. F. Mojica 1 , R. Pacheco 1 , C. Hernandez 1 , M. E. Vallejo 1,2 , J. P. Quinn 3 , Colombian Nosocomial Resistance Study Group; 1 CIDEIM, Cali, Colombia, 2 Univ. Pontificia Bolivariana, Medellin, Colombia, 3 Astra- Zeneca, Waltham, MA. 95 MONDAY SCIENTIFIC SESSIONS Final Program D-748 Evaluation of a New Eteststrip for KPC Detection in Enterobacteriaceae

D. Halimi

1 , D. Girlich 2 , P. Nordmann 2 , S. Rivat 1 , G.Durand 1 , R.Martelin 1 , G. Zambardi 1 ; 1 bioMerieux, La Balme les Grottes, France, 2 Hosp. Bicetre, Paris, France. D-749 Quantification of Modified Hodge Test: A New Approach to Phenotypic Screening of KPC

V. B. Ribeiro

1,2 , A. R. Linhares 1 , A. P. Zavascki 4 , A. L. Barth 1,2 ; 1 Univ. Fed- eral do Rio Grande do Sul, Porto Alegre, Brazil, 2 Hosp. de Clínicas de Porto Alegre, Porto Alegre, Brazil. D-750 Carbapenem-Resistant Enterobacteriaceae Detection and Prevention Practices, Michigan, 2012 B. M. Brennan, J. R. Coyle, M. S. Boehme, P. Boinapally, J. L. Finks; Michi- gan Dept. of Community Hlth., Lansing, MI. D-751 Comparison of Two Chromogenic Media and MacConkey Agar with Carbapenem Disks for the Detection of Carpaben- emase-Producing Gram Negative Rods in Simulated Stool Specimens

J. Blackburn

1 , C. Tsimiklis 1 , V. Lavergne 1 , J. Pilotte 2 , S. Grenier 2 , A. Gil- bert 2 , B. Lefebvre 2 , M. Domingo 2 , C. Tremblay 2 , A. Bourgault 2 ; 1 Hosp. du Sacré-Coeur de Montréal, Montréal, Canada, 2 Inst. Natl. de Santé Publique, Lab. de Santé Publique du Québec, Montréal, Canada. D-752 Performance of Ceftriaxone CAX Minimum Inhibitory Concentration MIC 1 μgml for Detection of Extended- Spectrum ơ-Lactamase ESBL Producing E. coli EC and K. pneumoniae KP

N. K. Boyd

1,2 , K. S. Alvarez 1,2 , L. Byrd 1 , K. P. Bhavan 1,2 ; 1 Parkland Hlth. and Hosp. System, Dallas, TX, 2 Univ. of Texas Southwestern Med. Sch., Dallas, TX. D-753 Rapid Detection of ơ-Lactamase Hydrolyzing Extended- Spectrum Cephalosporins in Enterobacteriaceae Using the New Chromogenic ơLACTA Test M. Garcia-Castillo, M. Morosini, M. Tato, T. Curiao, D. Gijón, A. Valverde, P. Ruiz-Garbajosa, R. Canton; Hosp. Univ. Ramón y Cajal, Madrid, Spain. D-754 Implementation of the 2010 Clinical and Laboratory Stan- dards Institute CLSI Guidelines: Effects on Antibiotic Abx Utilization in the Treatment of Presumed Extended-Spectrum Beta-Lactamase ESBL-Producing Enterobacteriaceae EB

J. Curello, C. MacDougall; UCSF Med. Ctr., San Francisco, CA. D-755