aureus aureus SA and its Small Colony Variants SCV Mutants J. Werth D. Hall D. Hall, B. J. Werth, J. P. McRoberts, M. J. Rybak; Wayne State Univ., Detroit, MI.

86 SCIENTIFIC SESSIONS September 9-12 | ------------------------------------------------------------------------ 082A Poster Session Glycopeptides and Lipopeptides: What’s New in PK PD and Safety Monday, 11:15 a.m.–1:15 p.m. . . . . . . . . . . . . . . . . . . . Halls A-C Presentations: A-596 Menadione-Dependent Small-Colony Variants of MRSA are Hypersusceptible to ơ-Lactams Intracellularly Due to Coopera- tion Between Vacuolar Acidic pH and Oxidant Species L. G. Garcia 1 , S. Lemaire 1 , B. C. Kahl 2 , K. Becker 2 , R. A. Proctor 3 , P. M. Tulkens 1 , F. Van Bambeke 1 ; 1 Univ. Catholique de Louvain, Brussels, Bel- gium, 2 Univ. Muenster, Muenster, Germany, 3 Univ. Wisconsin, Madison, WI. A-597 Activity of Antibiotics against a Normal Phenotype MRSA and its menD and hemB Mutants on Models of Bronchial Epithe- lial Cells Expressing Wild-Type, F508del or No CFTR L. G. Garcia 1 , B. C. Kahl 2 , K. Becker 2 , R. A. Proctor 3 , O. Denis 4 , P. M. Tulkens 1 , F. Van Bambeke 1 ; 1 Univ. Catholique de Louvain, Brussels, Bel- gium, 2 Univ. Muenster, Muenster, Germany, 3 Univ. Wisconsin, Madison, WI, 4 Univ. Libre de Bruxelles, Brussels, Belgium. A-598 Activity of Anti-Staphylococcal Antibiotics in an In Vitro Model of Biofilm Using Clinical Isolates of

S. aureus

J. Bauer 1 , M. Hallin 2 , O. Denis 2 , P. M. Tulkens 1 , F. Van Bambeke 1 ; 1 Univ. Catholique de Louvain, Brussels, Belgium, 2 Univ. Libre de Bruxelles, Brus- sels, Belgium. A-599 Influence of Oxidant Stress on the Activity of Antibiotics against the Extracellular and Intracellular THP-1 cells Forms of

S. aureus SA and its Small Colony Variants SCV Mutants

L. G. Garcia 1 , S. Lemaire 1 , B. C. Kahl 2 , K. Becker 2 , R. A. Proctor 3 , P. M. Tulkens 1 , F. Van Bambeke 1 ; 1 Univ. Catholique de Louvain, Brussels, Bel- gium, 2 Univ. Muenster, Muenster, Germany, 3 Univ. Wisconsin, Madison, WI. A-600 Evaluation of the Daptomycin DAP Mutant Selection Window in Vancomycin Resistant Enterococci VRE in a Phar- macokineticPharmacodynamic PKPD Model of Simulated Endocardial Vegetations SEV

B. J. Werth

1 , C. E. Ireland 1 , M. E. Steed 2 , M. J. Rybak 1 ; 1 Anti-Infective Res. Lab., Wayne State Univ., Detroit, MI, 2 Univ. Kansas Med. Ctr., Kansas City, KS. A-601 Evaluation of Daptomycin DAP Plus Ceftriaxone CTX against Vancomycin-Resistant Enterococcus faecium VREFm and faecalis VREFs in an In Vitro PharmacokineticPharmacody- namic PKPD Simulated Endocardial Vegetation SEV Model

A. D. Hall

1 , G. Sakoulas 2 , M. J. Rybak 1 ; 1 Wayne State Univ., Detroit, MI, 2 Univ of California San Diego, La Jolla, CA. A-602 A Preliminary Evaluation of the Novel Combination of Dap- tomycin Plus Fosfomycin FOS against Vancomycin-Resistant Enterococcus Using an In Vitro PharmacokineticPharmacody- namic PKPD Bacteremia Model

A. D. Hall, B. J. Werth, J. P. McRoberts, M. J. Rybak; Wayne State Univ., Detroit, MI.

A-603 Evaluation of the Novel Combination of High Dose Dapto- mycin HD-DAP plus TrimethoprimSulfamethoxazole TMP SMX against Daptomycin Non-susceptible DNS Methicillin- Resistant Staphylococcus aureus MRSA Using an In Vitro Phar- macokineticPharmacodynamic PKPD Model of Simulated Endocardial Vegetations SEVs M. E. Steed, B. J. Werth , C. E. Ireland, M. J. Rybak; Anti-Infective Res. Lab., Wayne State Univ., Detroit, MI. A-604 Impact of High Dose Daptomycin in Preventing Resistance Development in Viridans Group Streptococci

R. L. Akins; Univ. of Louisiana Monroe, Monroe, LA. A-605