Kruskal-Wallis Test
Test Statistics
a,b
hari_3 hari_6
hari_9 hari_12
Chi-Square 15.707
17.110 14.880
15.759 df
4 4
4 4
Asymp. Sig. .003
.002 .005
.003 a. Kruskal Wallis Test
b. Grouping Variable: Kelompok
Post Hoc Tests
Multiple Comparisons
Tukey HSD Dependent
Variable I
Kelompok J
Kelompok Mean
Difference I- J
Std. Error
Sig. 95 Confidence
Interval Lower
Bound Upper
Bound
hari_3 Aquades
100 -30.27600 11.56668
.105 -64.8878
4.3358 200
-29.86800 11.56668 .112
-64.4798 4.7438
300 10.91000 11.56668
.877 -23.7018
45.5218 Metformin
-32.80600 11.56668 .069
-67.4178 1.8058
100 Aquades
30.27600 11.56668 .105
-4.3358 64.8878
200 .40800 11.56668 1.000
-34.2038 35.0198
300 41.18600
11.56668 .015
6.5742 75.7978
Metformin -2.53000 11.56668
.999 -37.1418
32.0818
200 Aquades
29.86800 11.56668 .112
-4.7438 64.4798
100 -.40800 11.56668 1.000
-35.0198 34.2038
300 40.77800
11.56668 .016
6.1662 75.3898
Metformin -2.93800 11.56668
.999 -37.5498
31.6738
300 Aquades
-10.91000 11.56668 .877
-45.5218 23.7018
100 -41.18600
11.56668 .015
-75.7978 -6.5742
200 -40.77800
11.56668 .016
-75.3898 -6.1662
Metformin -43.71600
11.56668 .009
-78.3278 -9.1042
Metformin Aquades 32.80600 11.56668
.069 -1.8058
67.4178
Universitas Sumatera Utara
100 2.53000 11.56668
.999 -32.0818
37.1418 200
2.93800 11.56668 .999
-31.6738 37.5498
300 43.71600
11.56668 .009
9.1042 78.3278
hari_6 Aquades
100 -79.15400
16.74127 .001 -129.2502
-29.0578 200
-67.47600 16.74127
.005 -117.5722 -17.3798
300 -44.58800 16.74127
.096 -94.6842
5.5082 Metformin
-80.24600 16.74127
.001 -130.3422 -30.1498
100 Aquades
79.15400 16.74127
.001 29.0578
129.2502 200
11.67800 16.74127 .955
-38.4182 61.7742
300 34.56600 16.74127
.273 -15.5302
84.6622 Metformin
-1.09200 16.74127 1.000 -51.1882
49.0042 200
Aquades 67.47600
16.74127 .005
17.3798 117.5722
100 -11.67800 16.74127
.955 -61.7742
38.4182 300
22.88800 16.74127 .654
-27.2082 72.9842
Metformin -12.77000 16.74127
.938 -62.8662
37.3262 300
Aquades 44.58800 16.74127
.096 -5.5082
94.6842 100
-34.56600 16.74127 .273
-84.6622 15.5302
200 -22.88800 16.74127
.654 -72.9842
27.2082 Metformin
-35.65800 16.74127 .247
-85.7542 14.4382
Metformin Aquades
80.24600 16.74127
.001 30.1498
130.3422 100
1.09200 16.74127 1.000 -49.0042
51.1882 200
12.77000 16.74127 .938
-37.3262 62.8662
300 35.65800 16.74127
.247 -14.4382
85.7542
hari_9 Aquades
100 -89.20200
22.29543 .006 -155.9183
-22.4857 200
-86.24400 22.29543
.008 -152.9603 -19.5277
300 -66.81000
22.29543 .050 -133.5263
-.0937 Metformin
-90.62600 22.29543
.005 -157.3423 -23.9097
100 Aquades
89.20200 22.29543
.006 22.4857
155.9183 200
2.95800 22.29543 1.000 -63.7583
69.6743 300
22.39200 22.29543 .850
-44.3243 89.1083
Metformin -1.42400 22.29543 1.000
-68.1403 65.2923
200 Aquades
86.24400 22.29543
.008 19.5277
152.9603 100
-2.95800 22.29543 1.000 -69.6743
63.7583 300
19.43400 22.29543 .904
-47.2823 86.1503
Metformin -4.38200 22.29543 1.000
-71.0983 62.3343
300 Aquades
66.81000 22.29543
.050 .0937
133.5263 100
-22.39200 22.29543 .850
-89.1083 44.3243
200 -19.43400 22.29543
.904 -86.1503
47.2823 Metformin
-23.81600 22.29543 .820
-90.5323 42.9003
Universitas Sumatera Utara
Metformin Aquades
90.62600 22.29543
.005 23.9097
157.3423 100
1.42400 22.29543 1.000 -65.2923
68.1403 200
4.38200 22.29543 1.000 -62.3343
71.0983 300
23.81600 22.29543 .820
-42.9003 90.5323
hari_12 Aquades
100 -56.12200
10.47329 .000
-87.4620 -24.7820
200 -49.94200
10.47329 .001
-81.2820 -18.6020
300 -27.63200 10.47329
.101 -58.9720
3.7080 Metformin
-55.62600 10.47329
.000 -86.9660
-24.2860 100
Aquades 56.12200
10.47329 .000
24.7820 87.4620
200 6.18000 10.47329
.975 -25.1600
37.5200 300
28.49000 10.47329 .086
-2.8500 59.8300
Metformin .49600 10.47329 1.000
-30.8440 31.8360
200 Aquades
49.94200 10.47329
.001 18.6020
81.2820 100
-6.18000 10.47329 .975
-37.5200 25.1600
300 22.31000 10.47329
.246 -9.0300
53.6500 Metformin
-5.68400 10.47329 .982
-37.0240 25.6560
300 Aquades
27.63200 10.47329 .101
-3.7080 58.9720
100 -28.49000 10.47329
.086 -59.8300
2.8500 200
-22.31000 10.47329 .246
-53.6500 9.0300
Metformin -27.99400 10.47329
.094 -59.3340
3.3460 Metformin
Aquades 55.62600
10.47329 .000
24.2860 86.9660
100 -.49600 10.47329 1.000
-31.8360 30.8440
200 5.68400 10.47329
.982 -25.6560
37.0240 300
27.99400 10.47329 .094
-3.3460 59.3340
hari_15 Aquades
100 -49.75800
9.29904 .000
-77.5842 -21.9318
200 -48.33000
9.29904 .000
-76.1562 -20.5038
300 -21.51200
9.29904 .182
-49.3382 6.3142
Metformin -49.24600
9.29904 .000
-77.0722 -21.4198
100 Aquades
49.75800 9.29904
.000 21.9318
77.5842 200
1.42800 9.29904 1.000
-26.3982 29.2542
300 28.24600
9.29904 .046
.4198 56.0722
Metformin .51200
9.29904 1.000 -27.3142
28.3382
200 Aquades
48.33000 9.29904
.000 20.5038
76.1562 100
-1.42800 9.29904 1.000
-29.2542 26.3982
300 26.81800
9.29904 .062
-1.0082 54.6442
Metformin -.91600
9.29904 1.000 -28.7422
26.9102 300
Aquades 21.51200
9.29904 .182
-6.3142 49.3382
Universitas Sumatera Utara
100 -28.24600
9.29904 .046
-56.0722 -.4198
200 -26.81800
9.29904 .062
-54.6442 1.0082
Metformin -27.73400
9.29904 .051
-55.5602 .0922
Metformin Aquades
49.24600 9.29904
.000 21.4198
77.0722 100
-.51200 9.29904 1.000
-28.3382 27.3142
200 .91600
9.29904 1.000 -26.9102
28.7422 300
27.73400 9.29904
.051 -.0922
55.5602 . The mean difference is significant at the 0.05 level.
Homogeneous Subsets
hari_3
Tukey HSD
a
Kelompok N
Subset for alpha = 0.05 1
2 300
5 -27.3860
Aquades 5
-16.4760 -16.4760
200 5
13.3920 100
5 13.8000
Metformin 5
16.3300 Sig.
.877 .069
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
hari_6
Tukey HSD
a
Kelompok N
Subset for alpha = 0.05 1
2 Aquades
5 -44.1540
300 5
.4340 .4340
200 5
23.3220 100
5 35.0000
Metformin 5
36.0920 Sig.
.096 .247
Universitas Sumatera Utara
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
hari_9
Tukey HSD
a
Kelompok N
Subset for alpha = 0.05 1
2 Aquades
5 -43.8100
300 5
23.0000 200
5 42.4340
100 5
45.3920 Metformin
5 46.8160
Sig. 1.000
.820 Means for groups in homogeneous subsets are
displayed. a. Uses Harmonic Mean Sample Size = 5.000.
hari_12
Tukey HSD
a
Kelompok N Subset for alpha = 0.05 1
2 Aquades
5 3.6520
300 5
31.2840 31.2840
200 5
53.5940 Metformin 5
59.2780 100
5 59.7740
Sig. .101
.086 Means for groups in homogeneous subsets are
displayed. a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
hari_15
Tukey HSD
a
Kelompok N Subset for alpha = 0.05
1 2
3 Aquades
5 21.1160 300
5 42.6280 42.6280 200
5 69.4460 69.4460
Metformin 5 70.3620 70.3620
100 5
70.8740 Sig.
.182 .051
1.000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Daftar Pustaka
1. American Diabetes Association. Diagnosis and Classification of Diabetes
Mellitus. American Diabetes Association; 2012 2.
Piero M.N, Nzaro, G.M, Njagi J.M. Diabetes mellitus – a devastating metabolic disorder. Asian Journal of Biomedical and Pharmaceutical
Sciences 2014; 0440: 7-1.
3. Subroto MA. Ramuan herbal untuk diabetes mellitus. Jakarta: Penebar
Swadaya; 2006. p. 16-19. 4.
Adibe, M. O. Prevalence of Concurrent Use Of Herbal And Synthetic Medicines Among Outpatients In A Mission Hospital In Nigeria.
International Journal of Drug Development and Research 2009; 11: 66- 60.
5. Sudoyo AW, Setiyohadi B, Alwi I, Simadibrata M, Setiati S. Buku Ajar
Ilmu Penyakit Dalam Jilid III edisi V. Jakarta: Interna Publishing; 2009. 6.
Siregar B.L. Andaliman Zanthoxylum acanthopodium DC. di Sumatera Utara: deskripsi dan perkecambahan. Hayati 2002;101: 40-38.
7. Suarsana I.N, Priosoeryanto B.P, Wresdiyati T, Bintang M. Aktivitas Daya
Hambat Enzim α−Glukosidase dan Efek Hipoglikemik Ekstrak Tempe pada Tikus Diabetes. Jurnal Veteriner 2010;113: 195-190.
8. Ozougwu J.C, Obimba K.C, Belonwu C.D, Unakalamba, C.B. The
pathogenesis and pathophysiology of type 1 and type 2 diabetes mellitus. Academic journals 2013;44: 57-46.
9. Wijaya C.H, Triyanti I, Apriyantono A. Identification of volatile compounds and
key aroma compounds of andaliman fruit Zanthoxylum acanthopodium. J. Food Sci. Biotechnol 2002;116: 683-680.
10. Kristanty R.E, Mun’im A, Katrin. Isolation of antioxidant and xanthine
oxidase inhibitor from n-butanol extract of andaliman fruit Zanthoxylum acanthopodium. Int. j. Med. Arom 2012;23: 376-389.
11. Parhusip, A. J. N.,Sibuea. P, dan Tarigan. A. Studi Tentang Aktivitas
Antimikroba Alami Pada Andaliman. Seminar Nasional Teknologi Pangan. Jakarta;1999.p.190-193.
12. Robinson, T. Kandungan Organik Tumbuhan Tinggi. Bandung: Penerbit
ITB;1995.p.57-59. 13.
Waheed A, Mahmud S, Akhtar M, Nazir T. Studies on the components of essential oil of Zanthoxylum armatum by GC-MS. American Journal Of
Analytical Chemistry 2011;2: 258-261.
14. Campbell NA and Jane B. Reece. Biology. 10
th
ed. USA: Pearson education, inc; 2014.p. 9.
15. Guyton, A.C. and Hall, J.E. Textbook of Medical Physiology. 11
th
ed. USA; Elsevier Inc; 2006.p. 831-836.
16. Himawan I.W, Pulungan A.B, Tridjaja B, Batubara R.L. Komplikasi
jangka pendek dan jangka panjang diabetes mellitus tipe 1. Sari pediatri 2009;106: 372-367.
Universitas Sumatera Utara
17. Ditjen Bina Farmasi dan Alkes. Pharmaceutical Care untuk penyakit
Diabetes Mellitus. Jakarta: Departemen Kesehatan RI; 2005.p. 9, 29, 30, 32, 39, 43
18. PERKENI. Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 di
Indonesia. Perkumpulan Endokrinologi Indonesia; 2011. 19.
Yanling W, Yanping D, Yoshimasa T, Wen Z. Risk factors contributing to type 2 diabetes and recent advances in the treatment and prevention. Int. J.
Med. Sci 2014;1111: 1200-1185.
20. Szkudelski T. The mechanism of alloxan and streptozotocin action in B
cells of the rat pancreas. Physiol. Res 2001; 50: 546-536. 21.
Gupta D.D, Mandi S.S. Species Specific AFLP Markers for authentication of Zanthoxylum acanthopodium Zanthoxylum oxyphyllum. Journal of
Medicinal Plants Studies 2013;16: 1-9.
22. Gultom S. Flavonoid Buah Andaliman Zanthoxylum acanthopodium DC
sebagai antioksidan dan inhibitor α-glukosidase. Thesis. Bogor: Program Pascasarjana IPB; 2011.
23. Lenzen S. The mechanisms of alloxan and streptozotocin-induced
diabetes. Diabetologia 2008;51: 226-216. 24.
Hayati F, Widyarini, S, Helminawati. Efek antihiperglikemia infusa kangkung darat Ipomoea reptans Poir terhadap kadar glukosa darah
mencit jantan yang diinduksi streptozotocin. Jurnal Ilmiah Farmasi 2010;71: 22-13.
25. Depkes RI. Cara Pembuatan Simplisia. Jakarta: Departemen Kesehatan
Republik Indonesia;1985.p.2-24. 26.
Ditjen POM.Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Departemen Kesehatan RI;2000.p. 3-5, 10-11
27. World Health Organization. General Guidelines for Methodologies on
Research and Evaluation of Traditional Medicine. Geneva: WHO; 2001 28.
Tensiska. Aktivitas Antioksidan Ekstrak Buah Andaliman Zanthoxylum acannhopodium DC dalam Beberapa Sistem Pangan dan Kesetabilan
Aktivitasnya Terhadap Kondisi Suhu dan pH. Thesis. Bogor: Program Pascasarjana IPB;2001.
29. Andayani. Kajian Daya Insektisida Alami Nabati Kulit Buah Manggis
Garcinia mangostan, Buah Andaliman Zanthoxylum acanthopodium DC, Getah Gambir Uncaria gambir Roxb dan Daun Teh Camellia
sinensis L Terhadap Perkembangan Hama Gudang Sitophilus zeamays otsh. Skripsi. Bogor: Fakultas Teknologi pertanian IPB; 2000.
30 Robinson, T. Kandungan Organik Tumbuhan Tinggi. 6
th
ed. Bandung: Institut Teknologi Bandung;1995.p.191
31. Golbidi S, Ebadi A, Laher I. Antioxidants in the treatment of diabetes.
Current Diabetes Reviews 2011;7: 125-106. 32.
Setiawan B, Suhartono E. Stres oksidatif dan peran antioksidan pada diabetes mellitus. Maj Kedokt Indon 2005;552: 91-86.
33. Kristanty RE, Suriawati J. Cytotoxic and antioxidant activity of petroleum
extract of andaliman fruits Zanthoxylum acanthopodium DC.. International Journal of PharmTech Research 2014;63: 1069-1064.
Universitas Sumatera Utara
34. Jiang Y, Huang W, Wang J, Xu Z, He J. Metformin plays a dual role in
MIN6 pancreatic β cell function trough AMPK-dependent autophagy. Int. J. Biol. Sci 2014;103: 277-268.
35. Hur KY, Lee M. New mechanisms of metformin action: Focusing on
mitochondria and the gut. J Diabetes Investig 2015;6: 609-600. 36.
Klip A, Leiter LA. Cellular mechanism of action of metformin. Diabetes care 2013;136: 704-694.
37. Rohilla A, Ali S. Alloxan induced diabetes : mehchanisms and effects.
International journal of research in pharmaceutical and biomedical sciences 2012;32:823-819.
38. Diandra. Pengaruh Pemberian Infusa Herba Sambiloto Andrographis
paniculata Nees Terhadap Glibenklamid Dalam Menurunkan Kadar Glukosa Darah Tikus Putih Jantan Yang Dibuat Diabetes. Depok: Fakultas
Matematika da Ilmu Pengetahuan Alam UI; 2011.
39. Katzung BG. Farmakologi dasar dan klinik. Jakarta: Salemba Medika;
2002. p 677-678. 40.
Raju SB, Rao, GB, Latha MY. Anti-hyperglycemic activity of Hygrophila spinosa roots in alloxan-induced diabetic rats. Int. J. Pharm Ind. Res
2011;14: 317-315.
41. Ramaiah A. Antidiabetic activity of methanolic extract of Memecylon
Malabrium Cogn Melastomataceae Leaves. International Journal of Phytopharmacy 2013;21:12-9.
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19
BAB 3 KERANGKA TEORI, KERANGKA KONSEP DAN HIPOTESIS
3.1 Kerangka Teori
