3.5. Bagan Penelitian
3.5.1. Bagan Alir Uji Biodegradasi Edible Film Pati Ubi Kayu dengan
Penambahan Serbuk Batang Ubi Kayu dalam Tanah
Dicampur Homogen
Dipanaskan sampai mengental pada suhu100°C
Dicetak di atas kaca Dikeringkan dianginkan
Dipotong-potong Ditimbang
Uji Biodegradasi Hasil Campuran
Bentuk film 10g Pati ubi kayu + Serbuk
Batang Ubi kayu 0,5g 100 g Air + 1 g Gliserol
Massa spesimen
ditimbang Analisis
SEM Uji FT-IR
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Universitas Sumatera Utara
3.5.2. Bagan Alir Uji Biodegradasi Edible Film Pati Ubi Kayu dengan
Penambahan Serbuk Batang Ubi Kayu dalam Media Aspergillus Niger
Dicampur Homogen
Dipanaskan sampai mengental pada suhu100°C
Dicetak di atas kaca Dikeringkan dianginkan
Dipotong-potong Ditimbang
Uji Biodegradasi Hasil Campuran
Bentuk film 10g Pati ubi kayu + Serbuk
Batang Ubi kayu 0,5g 100 g Air + 1 gr Gliserol
Massa spesimen
ditimbang Analisis
SEM Uji FT-IR
Media bermikroba A.Niger
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Universitas Sumatera Utara
3.5.3. Bagan Alir Uji Toksisitas Edible Film Pati Ubi Kayu
BAB 4 HASIL DAN PEMBAHASAN
Media NA
Dituang kedalam cawan petri
Didiamkan hingga padat
E.Coli digoreskan
Diamati Zona Bening
Sampel diletakkan di atas biakan E. coli
Diinkubasi pada suhu 37
o
C selama 48 jam
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Universitas Sumatera Utara