Perendaman NaCl 57.00 42.50 Total Not corrected for ties. b. Grouping Variable: Perlakuan 21.00 Total Not corrected for ties. b. Grouping Variable: Perlakuan 21.00 Rancangan Penelitian Sampel dan Besar Sampel Penelitian .1 Sampel Penelitian

Test Statistics b Candida albicans Mann-Whitney U 5.500 Wilcoxon W 26.500 Z -2.091 Asymp. Sig. 2-tailed .037 Exact Sig. [21-tailed Sig.] .041 a

a. Not corrected for ties. b. Grouping Variable: Perlakuan

• PADA KELOMPOK A DAN D Ranks Perlakuan N Mean Rank Sum of Ranks Candida albicans Perendaman Vinegar Apel 5 6 3.50

21.00 Perendaman NaCl

0,9 6

9.50 57.00

Total 12 Test Statistics b Candida albicans Mann-Whitney U .000 Wilcoxon W 21.000 Z -2.908 Asymp. Sig. 2- tailed .004 Exact Sig. [21- tailed Sig.] .002 a Universitas Sumatera Utara • PADA KELOMPOK B DAN C Ranks Perlakuan N Mean Rank Sum of Ranks Candida albicans Perendaman Vinegar Apel 6,23 6

7.08 42.50

Perendaman Sodium Hipoklorit 1 6 5.92

35.50 Total

12 Test Statistics b Candida albicans Mann-Whitney U 14.500 Wilcoxon W 35.500 Z -.632 Asymp. Sig. 2-tailed .527 Exact Sig. [21-tailed Sig.] .589 a

a. Not corrected for ties. b. Grouping Variable: Perlakuan

• PADA KELOMPOK B DAN D Ranks Perlakuan N Mean Rank Sum of Ranks Candida albicans Perendaman Vinegar Apel 6,23 6

3.50 21.00

Perendaman NaCl 0,9 6 9.50

57.00 Total

12 Universitas Sumatera Utara Test Statistics b Candida albicans Mann-Whitney U .000 Wilcoxon W 21.000 Z -2.908 Asymp. Sig. 2-tailed .004 Exact Sig. [21-tailed Sig.] .002 a

a. Not corrected for ties. b. Grouping Variable: Perlakuan

• PADA KELOMPOK C DAN D Ranks Perlakuan N Mean Rank Sum of Ranks Candida albicans Perendaman Sodium Hipoklorit 1 6

3.50 21.00

Perendaman NaCl 0,9 6 9.50

57.00 Total

12 Test Statistics b Candida albicans Mann-Whitney U .000 Wilcoxon W 21.000 Z -2.934 Asymp. Sig. 2-tailed .003 Exact Sig. [21-tailed Sig.] .002 a Universitas Sumatera Utara Lampiran 4 Surat Keterangan Selesai Penelitian Laboratorium Mikrobiologi FMIPA USU Universitas Sumatera Utara DAFTAR PUSTAKA 1. McCabe JF, Walls AWG. Applied dental materials. 9 th ed., London: Blackwell, 2008: 110-23. 2. The Academy of Prosthodontics. The glossary of prosthodontic terms. The J Prosthetic Dent 2005; 941: 30-1. 3. Carr AB, et al. McCracken’s removable partial prothodontics. 11 th ed., Saint Louis: Elsevier Mosby, 2005: 8-9. 4. Rueggeberg FA. Dental materials for complete dentures. In: Rahn AO, et al. eds. Textbook of complete denture, 6 th ed., USA: People’s Medical Publishing House, 2006: 8-10. 5. Tandon R, Gupta S, Agarwal SK. Denture base materials: Form past to future. IJDS 2010; 22: 33-5. 6. Groover MP. Fundamentals of modern manufacturing: Materials, processes, and systems. 3 rd ed., USA: John Wiley Sons Inc., 2007: 145-66. 7. O’brien WJ. Dental materials and their selection. 3 rd ed., USA: Quintessence Publishing Co., 2002: 141-53. 8. Diwan RR. Materials prescribed in the management of edentulous patients. In: Zarb, et al. eds. Prosthodontic treatment for edentulous patients: Complete dentures and implant-supported prostheses, 12 th ed., Saint Louis: Elsevier Mosby, 2003: 190-6. 9. Anusavice KJ. Phillips’ science of dental materials. 11 th ed., Saint Louis: Saunders Elsevier, 2003: 75-101, 88-92, 143-6. 10. Van Noort R. Introduction to dental materials. 2 nd ed., London: Elsevier, 2002: 211-7. 11. Craig RG, et al. Dental materials properties and manipulation. 7 th ed., Saint Louis, Missouri: Mosby, 2002: 258-65. 12. Young BC. A comparison of polymeric denture base materials. Thesis. University of Glasgow, 2010: 10-23. Universitas Sumatera Utara 13. Dwiatmoko S, Kristiana D. Pengaruh komuniksi kesehatan secara lisan dan tulisan terhadap pengetahuan, sikap dan kebersihan gigi tiruan para pemakai gigi tiruan lepasan. Dentika Dent J 2011; 161: 14-7. 14. Arici N, Ural C. The effects of a denture cleanser on the surface roughness of heat-cured and cold-cured acrylic resins. Turkish J Orthod 2013; 262: 92-7. 15. Mota ACLG, Castro RD, Oliveira JA, Lima EO. Antifungal activity of apple cider vinegar on Candida species involved in denture stomatitis. Journal of Prosthodontics 2015; 42: 296-302. 16. de Castro RD, Mota ACLG, Lima EO, Batista AUD, Oliveira JA, Cavalcanti AL. Use of alkohol vinegar in the inhibition of Candida spp. and its effect on the physical properties of acrylic resins. BMC Oral Health 2015; 1552: 1-7. 17. Pinto TMS, Neves ACC, Leao MVP, Jorge AOC. Vinegar as an antimicrobial agent for control of Candida spp. in complete denture wearers. J Appl Oral Sci 2008; 166: 385-90. 18. Sousa FACG, Paradella TC, Koga-Ito CY, Jorge AOC. Effect of sodium bicarbonate on Candida albicans adherence to thermally activated acrylic resin. Braz Oral Res 2009; 234: 381-5. 19. Faot F, et al. Efficacy of citric acid denture cleanser on the Candida albicans biofilm formed on polymethyl methacrylate: effects on residul biofilm and recolonization process. BMC Oral Health 2014; 14: 77. 20. Jafari AA, Falah-Tafti A, Lotfi-Kamran MH, Zahraeii A, Kazemi A. Vinegar as a removing agent of Candida albicans from acrylic resin plates. Jundishapur J Microbiol 2012; 52: 388-92. 21. Anibal P.C., Sardi JCO, Peixoto ITA, Moraes JJC, Hofling JF. Conventional and alternative antifungal therapies to oral candidiasis. Brazilian Journal of Microbiology 2010; 41: 824-31. 22. Komiyama EY, Back-Brito GN, Balducci I, Koga-Ito CY. Evaluation of alternative methods for the disinfection of toothbrushes. Braz Oral Res 2010; 241: 28-33. Universitas Sumatera Utara 23. Pisani MX, Silva CHL, Paranhos HFO, Souza RF, Macedo AP. The effect of experimental denture cleanser solution Ricinus communis on acrylic resin properties. Materials Research 2010; 133: 369-73. 24. Kumar MN, Thippeswamy HM, Raghavendra Awamy KN, Gujjari AK. Efficacy of commercial and household denture cleansers against Candida albicans adherent to acrylic denture base resin: an in vitro study. Indian J Dent Res 2012; 23: 39-42. 25. Yadav R, Yadav VS, Garg S, Mittal S, Garg R. Effectiveness of different denture cleansing methods on removal of biofilms formed in vivo. J Cranio Max Dis 2013; 21: 22-7. 26. Naeem A, Amrit R, Sumit M, Nisha S, Pankaj K, Taseer B. Denture hygiene: A short note on denture cleansers. Journal of Science 2015; 53: 131-3. 27. David, Munadziroh E. Perubahan warna lempeng resin akrilik yang direndam dalam larutan desinfektan sodium hipoklorit dan klorhexidin. Maj Ked Gigi Dent J 2005; 381: 36-40. 28. Joseph RM. Comparison of efficacy of sodium hypochlorite with sodium perborate in the removal of stains from heat cured clear acrylic resin. The Journal of Indian Prosthodontic Society 2009; 91: 6-11. 29. Uludamar A, Özkan YK, Kadir T, Ceyhan I. In vivo efficacy of alkaline peroxide tablets and mouthwashes on Candida albicans in patients with denture stomatitis. J Appl Oral Sci 2010; 183: 291-6. 30. Zarb, et al. Prosthodontic treatment for edentulous patients: Complete dentures and implant supported prosthesis. 13 th ed., Elsevier Mosby, 2013: 133-9,152-5. 31. Fukuzaki S, Urano H, Yamada S. Effect of pH on the efficacy of sodium hypochlorite solution as cleaning and bactericidal agents. Research Papers 2007; 588: 465-9. 32. da Silva PMB, et al. Effectiveness of six different disinfectants on removing five microbial species and effects on the topographic characteristics of acrylic resin. J Prosthodont 2010; 17: 627-33. Universitas Sumatera Utara 33. Mey Bengkulen. Dunia biosains. http:dunianyabiosains.blogspot.com200901 sejuta-pesona-buah-apel.html. 28 Januari 2009. 21 September 2015. 34. Kwartiningsih E, Mulyati NS. Fermentasi sari buah nanas menjadi vinegar. Jurusan Teknik Kimia Fakultas Teknik UNS 2005; 41: 8-12. 35. International Symposium. Book of abstracts: Vinegars and acetic acid bacteria. Reggio Emilia Italy 2005: 84. 36. Anonymous. Produk fermentasi buah anggur, cider, dan vinegar. http:tekpan. unimus.ac.idwp-contentuploads201307PRODUK-FERMENTASI-BUAH- ANGGUR-CIDER-DAN-VINEGAR.pdf 37. Karim NM. Perbandingan efektivitas cuka apel dan dietilpropion terhadap penurunan berat badan tikus Rattus novergicus. Skripsi. Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2011: 4-9. 20 September 2015. 38. Ostrosky EA, Mizumoto MK, Lima M.E.L., Kaneko TM., Nishikawa SO., Freitas BR. Métodos para avaliação da atividade antimicrobiana e determinação da Concentração Mínima Inibitória CMI de plantas medicinais. Braz J Pharmacogn 2008; 182: 301-7. 39. Zubaidah E, Rosdiana I. Efektivitas cuka salak dan cuka apel terhadap kadar glukosa darah dan histopatologi pankreas tikus diabetes. Jurnal Pangan dan Agroindustri 2016; 41: 170-9. 40. Ratnasari A, Wahjuni W, Hendrijantini N. Efek seduhan bunga rosella dalam menghambat pertumbuhan Candida albicans pada resin akrilik. Journal of Prosthodontics 2013; 41: 22-6. 41. Moersidi SNM. Daya hambat minimal ekstrak kulit apel manalagi terhadap pertumbuhan Candida albicans. Skripsi. Surabaya: Universitas Hasanuddin, 2015: 1-3, 9-12. 42. Khindria S.K., Mittal S, Sukhija U. Evolution of denture base materials. J Indian Prosthodont Soc 2009; 92: 64-9. 43. Park HW, Kim CW, Kim YS. A comparative study on the accuracies of resin denture bases and metal denture bases. J Korean Acad Prosthodont 2001; 393: 250-6. Universitas Sumatera Utara 44. Gurbuz O, Unalan F, Dikbas I. Comparison of the tranverse strength of six acrylic denture resins. OHDMBSC 2010; 91: 21-4. 45. Rashid AA. Evaluation of the hardness of cold cured acrylic resin material by processing at different temperature. Iraqi National J of Nursing Specialities 2011; 242: 44-51. 46. Ferracane JL. Materials in dentistry. 2 nd ed. Philadelphia: Lippincott Williams Wilkins, 2001: 262-73. 47. Naveen BH, Patil SB., Kumaraswamy K. A study on transverse strength of different denture base resind repaired by various materials an methods: An in vitro study. J Dent Sci 2010; 11: 66-73. 48. Kostoulas I, Kavoura VT, Frangou MJ, Polyzois GL. Fracture force, deflection and toughness of acrylic denture repairs involving glass fiber reinforcement. Journal of Prosthodontics 2008; 17: 257-61. 49. Hilgenberg SP, Oreliana-Jimenez EE, Sepulveda-Navarro WF, et al. Evaluation of surface physical properties of acrylic resins for provisional prosthesis. Mat Res 2008; 113: 257-60. 50. Sugianitri N K. Ekstrak biji buah pinang Areca Catechu L. dapat menghambat pertumbuhan koloni Candida albicans secara in vitro pada resin akrilik heat cured. Tesis. Denpasar: Fakultas Kedokteran Gigi Universitas Udayana, 2011. 51. Pereira-Cenci T, Del Bel Cury AA, Crielaard W, Ten Cate JM. Development of Candida-associated denture stomatitis: New insights. J Appl Oral Sci 2008; 162: 86-94. 52. Jubhari EH, Putri NDU. Tingkat pemahaman terhadap instruksi cara pembersihan gigi tiruan lepasan pada pasien Fakultas Kedokteran Gigi Universitas Hasanuddin. Jurnal PDGI 2014; 632: 54-7. 53. Anonymous. Aneka vinegar si anggur asam. http:foodservicetoday.co.idpage contentaneka_vinegar_si_anggur_asamIngredient-Focus25 Oktober 2015. 54. Jhonston CS, Gaas CA. Vinegar: Medical uses and antiglycemic effect. MedGenMed. 2006; 82: 61. Universitas Sumatera Utara 55. Animated-Teeth.com. Homemade denture cleansers. http:www.animated teeth.comfalse-teeth.coma-homemade-denture-cleanser.htm. 2015 56. Supranto, J. 2001. Teknik Sampling untuk Survei dan Eksperimen. Jakarta: PT Rineka Cipta, 2001. 29 November 2015. Universitas Sumatera Utara

BAB 3 METODE PENELITIAN

3.1 Rancangan Penelitian

Penelitian ini merupakan jenis penelitian eksperimental laboratoris dengan menggunakan desain post test only control group. 3.2 Sampel dan Besar Sampel Penelitian 3.2.1 Sampel Penelitian Sampel pada penelitian ini menggunakan RAPP dengan bentuk lempeng uji berukuran 10 mm x 10 mm x 1 mm sesuai dengan ANSIADA Standard No.12 tahun 2002 Gambar 5. 40 Gambar 5. Bentuk dan ukuran sampel

3.2.2 Besar Sampel Penelitian

Jumlah sampel penelitian ditentukan berdasarkan rumus Federer: 56 t - 1 r - 1 ≥ 15 Keterangan: t: jumlah perlakuan r: jumlah ulangan Penelitian ini terdapat 4 kelompok sampel untuk setiap pengujian, maka t = 4 dan jumlah sampel r setiap kelompok dapat ditentukan sebagai berikut: 1 mm 10 mm 10 mm Universitas Sumatera Utara 4 - 1 r - 1 ≥ 15 3r - 1 ≥ 15 3r – 3 ≥ 15 3r ≥ 15 + 3 3r ≥ 18 r ≥ 183 r ≥ 6 r = 6 Jumlah sampel untuk masing-masing kelompok adalah 6, maka jumlah keseluruhan sampel sebanyak 24 buah lempeng uji. 3.3 Variabel Penelitian dan Definisi Operasional 3.3.1 Klasifikasi Variabel

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