Conclusion Objectives 1. To share lesson learned and best practices among alumni on bio-

24 In the first generation of reproductive biotechnology, artificial insemination has been widely applied to improve genetic quality in breeding system. The technology is very familiar also in animal husbandry either in beef cattle or the dairy cattle. This technology has been supported by providing huge number of frozen semen by the two big Artificial Insemination Center in Lembang West Java and Singosari East Java. Even several provinces has also established local artificial insemination center to support the application of AI technology. However, even this technology has been contributed to improve farm animal population but farm animal especially beef and dairy cattle populaton are still low. It is therefore, it is still required to develop technology to accellerate animal populaton and to improve genetic quality. It seems that next generation of biotechnology such as embryo transfer have good prospect to increase farm animal population.Embryo transfer required availability of embryo to be transfered. There are two types of technology to provide embryo for embryo transfer technology namely from in vivo embryo production by MOET multiple ovulaton and embryo tansfer and in vitro embryo production using slaughterhouse material and from live donor by Ovum Pick Up OPU technology. This paper will not give an in depth methodical approach to these technologies, but a brief description of acchievement of our laboratory on in vitro embryo production in Indonesia.

2. Results and Discussion

2.1. Sheep in vitro embryo production

Research on in vitro embryo development has been conducted in our laboratory since year 2000, with mainly using sheep and bovine oocytes. Focus research on in vitro sheep embryo production were begun by several researchs on improvement of oocytes maturation as basic research. Research was initiated by using different type medum [1] with several suplementation into maturation medium to improve capability of oocyte to become matured, fertilized and developed toward early embryonic development [2]. Several research on improvement ability of oocyte to be fertilized and early embryonic development such as influence of several medium [2] and supplementation of medium with an antioksidant such as Glutathion [3, 4], α-tocoferol [5], β-mercaptoethanol [6], and sericin [7] to improve efficient of in vitro embryo production. Another research has been conducted also to anlyse influence time of transportation on oocyte quality [8]. This result