Root leght Result The result of Analysis of variance of the treatments showed highly
126 nitrogen, since nitrogen is used for the growth of cells, tissues and organs
of plants. This is in accordance with the opinion of Afriani [5]. Based on the observation parameters in Table 4.1.3 and Table 4.1.4 shows
that treatment of H4 resulted in the highest root length namely 2.35 cm and the best plantlet fresh weight namely 3.53 g. It is probably caused in H4
medium containing macro and micro nutrients, glucose, vitamins and amino acids for proper growth of root length and fresh weight of plantlets, in
addition to the calcium content and the type of nitrogen also affects the growth of root length and fresh weight of plantlets. It is appropriate with the
opinion of Afriani [5] and Nurheldi [6] that nitrogen in the form of nitrate NO3
-
is better for growth of explants. Gandasil and Growmore fertilizer containing 52 of total nitrogen and 20 of them is in the nitrate form.
Besides in the form of nitrate, fertilizer and Gandasil and Growmore containing nitrogen in the form of ammonium NH4
+
namely 32 of the total N.
Calcium plays a role in cell growth of plants, but it also plays a role in calcium to strengthen, organize penetrating power, and take care of the cell
wall. Calcium also plays a role in the formation of roots, especially on the growing point of the root [7]. According to Kishi and Tagaki [8], nitrogen
in the form of ammonium is also needed in the growth of the explants, and the availability of other nutrients in the medium with addition of fertilizer
like Gandasil and Growmore can spur the growth of root length and fresh weight of plantlets.