Materials and Methods Dianita

131 This experiment found that there was no significant effect of treatments on phosphorous content in shoot, root and soils Table 3. The use of T. harzianum and A. niger in phospho-compost decomposition able to solubilize more P, because T. harzianum and A. niger is an organic acid- producing organisms under aerobic conditions. P is derived from rock phosphate more soluble in acidic conditions. The mineralization of insoluble P forms by organic acids are the main advantages of composting rock phosphate [10]. Table 3. P-Total in Shoot, Root and Soils at the End of Experiment Treatments P Total Shoot Root Soils ppm Rock Phosphate RP 0.34 0.11 120.94 Phokos 0.37 0.14 123.00 Triphos 0.35 0.12 159.44 Asphos 0.34 0.13 139.84 Table 4. P Solubilizing Fungi in soils at the End of Experiment Treatments P Solubilizing Fungi CFUg Rock Phosphate RP 2.0 X 10 3 Phokos 1.5 X 10 3 Triphos 3.6 X 10 4 Asphos Composite analyses from Soil Biotechnology Laboratory, the Faculty of Agriculture, IPB 2012 Composite analyses for PSF in soils media at the end of experiment showed that there was PSF in RP, Phokos and Trichos treatments but not in Asphos treatment Table 4. The amount of PSF in soils of Triphos treatment was the highest. Trichoderma fungi in nature favors temperatures between 15- 32ºC and pH in a range of 5.5 to 8.5. Trichoderma actively takes over a root zone and makes it difficult for pathogens to compete for space on the roots and for nutrients [11].

4. Conclusion

Application of phospho-compost with and without additional microorganism of T. harzianum and A. niger R1, R2, R3 significantly resulted a better growth and P content of S.splendida than rock phosphate R0 treatment. 132 Acknowledgement This experiment was funded by Operational Fund for State Higher Education with Group Research Scheme, according to letter of Agreement No. 209UN21.6PL2012, date: 09 August 2012.

5. References

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