e. Kadar abu tidak larut asam
Sampel ulangan A gram
B gram C gram
Kadar abu TLA
rata- rata
Cymodocea rotundata
1 5,01
26,54 26,54
2 5,15
28,07 28,07
Keterangan : A = bobot sampel gram
B = bobot cawan kosong gram C = bobot sampel + cawan setelah ditanur gram
Kadar abu TLA ulangan 1 = 26,54
− 26,54 5,01
x 100 = 0 Kadar abu TLA ulangan 2 =
28,07 − 28,07
5,15 x 100 = 0
Kadar abu TLA rata − rata =
0 + 0 2
= 0
f. Kadar karbohidrat by difference
Kadar karbohidrat = 100 - kadar air + kadar abu + protein + lemak
= 100 - 90,6655 + 2,3545 + 1,1291 + 0,7907 = 5,0602
Lampiran 3 Perhitungan Rendemen Ekstrak Kasar
Pelarut Ulangan A gram
B gram C gram Rendemen
Rata-rata Metanol
1 20
37,5326 39,5611
10,14 9,76
2 20
39,512 41,3862
9,37 Etil
Asetat 1
20 37,7719
37,9077 0,67
0,57 2
20 37,4393
37,5315 0,46
n- heksana
1 20
36,5962 36,6096
0,07 0,16
2 20
37,8394 37,8884
0,25 Keterangan :
A = bobot awal sampel gram B = bobot botol kosong gram
C = bobot sampel+botol setelah ekstraksi gram
a. Metanol
Rendemen ulangan 1 = C
− B A
x 100 = 39,5611
− 37,5326 20
x 100 = 10,14 Rendemen ulangan 2 =
C − B
A x 100 =
41,3862 − 39,512
20 x 100 = 9,37
Rendemen rata − rata =
10,14 + 9,37 2
= 9,76
b. Etil Asetat
Rendemen ulangan 1 = C
− B A
x 100 = 37,9077
− 37,7719 20
x 100 = 0,67
Rendemen ulangan 2 = C
− B A
x 100 = 37,5315
− 37,4393 20
x 100 = 0,46
Rendemen rata − rata =
0,67 + 0,46 2
= 0,57
c. N-heksana
Rendemen ulangan 1 = C
− B A
x 100 = 36,6096
− 36,5962 20
x 100 = 0,07
Rendemen ulangan 2 = C
− B A
x 100 = 37,8884
− 37,8394 20
x 100 = 0,25
Rendemen rata − rata =
0,07 + 0,25 2
= 0,16
Lampiran 4 Perhitungan Kadar Serat Pangan a.
Kadar serat pangan tak larut IDF
Nama Sampel
A B
C D
E IDF
g100g Rata-rata
g100g
Cymodocea rotundata
0,2812 0,7014 0,7341 17,6691 17,6832 5,33
5,39 0,4149 0,7125 0,7509 16,3306 16,3428
5,45 Blanko
0,6954 0,7021 15,2121 15,2153 0,0035
0,0036 0,7236 0,7301 16,9415 16,9443
0,0037 Keterangan :
A = Berat sampel gram
B = Berat kertas saring kosong gram
C = Berat kertas saring + residu setelah dioven gram
D = Berat cawan porselen kosong gram
E = Berat cawan porselen + abu setelah ditanur gram
IDF 1 g100g =
C − B − E − D − Blanko A
x 100 IDF 1
g100g = 0,7341 − 0,7014 − 17,6832 − 17,6691 − 0,0036
0,2812 x 100
= 5,33 g100 g sampel
IDF 2 g100g =
C − B − E − D − Blanko A
x 100 IDF 2
g100g = 0,7509 − 0,7125 − 16,3428 − 16,3306 − 0,0036
0,4149 x 100
= 5,45 g100 g sampel Rata
− rata IDF g100g = 5,33 + 5,45
2 = 5,39
b. Kadar serat pangan larut SDF
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