ISSN 2086-5953 which can produce enzymes that can ligniselulase
ligniselulosa hydrolyze lignin and sugar into the next by bacteria [11]. The same is fermented into
ethanol. The use of beneficial bacteria Another breed that is easy to, so as to produce enough
ethanol Clostridium thermocellum buying once it will be cheaper. Moreover with more quantity will
be the formation of ethanol faster.
1.1 Goal
The goal of scientific writing this time is to provide a alternative solutions for the impact
caused by the production of waste gelatin of seaweed species Gracillaria sp. with a bioethanol
used as a source of renewable energy. Also, to minimize the wastewater treatment from the three
stages hydrolysis, fermentation, and distillation into one step by using the bacterium Clostridium
themocellum.
1.2 Problem formulation
The formulation of the problem is the reference in the writing this time starting from the
potential production of waste gelatin which are not being utilized optimal. One of the womb that can be
used are cellulose, the material that can be used in producing
bioethanol. Typically,
production Bioethanol is mechanically less efficient, and
therefore need to be a development towards the use of bacteria in this case, Clostridium themocellum
to summarize the course of bioethanol production and processing.
1.3 Benefit
In general, the benefits of this paper is divided into three objects. First, the benefits to the
environment will have an impact towards the improvement of waste generated by the production
of these gelatinous. Second, benefits society is a concept that can be developed to open a related
jobs. Third, the benefit for government is to accommodate and considerations for developing
alternative energy ready to be applied nationally. Fourth, the benefits for students are as self-
development tools and container as supporting creativity.
2 DISCUSSION
2.1 Materials and Equipment
Equipment and materials used
when producing bioethanol by one stage method, actually
not too different from the three production methods stage, because the principle of making the same
[10]. Its just that distinguishes is the role of bacteria in it. The materials used in this production
process such waste gelatin and culturing the bacterium Clostridium thermocellum. Meanwhile, a
tool used in bioethanol production is processing machines which mainly consists of:
1. Space reactor and stirrer
This space serves as a breeding room and maintenance of bacterial Clostridium thermocellum.
Mixer function, among others, to maintain immobilization of bacteria and aeration process
similar to the function stirrer in a bioreactor. In addition, it should also be guard-condition
conditions required by the bacteria to grow and develop among others, setting the optimal pH,
water content, temperature and pressure.
2. Space heating
Space heating is a place of burning to obtain heat energy that serves as a support for the process
of distillation. This heat can generated by steam generator boiler or from direct flame heating.
However, Such temperature conditions necessary arrangements will be stable and easy to set in
accordance with what is needed by reactors [12].
3. Vacuum pump
In this case, serves as a vacuum suction pump ethanol gas generated from distillation to take
to the condensation device condenser [12]. Manually, this tool can be dispensed with the
assumption that ethanol gas generated will be able to make the shift to the condenser, although not as
effective if you use the vacuum pump.
4. Condenser
Is a tool used to cool the ethanol gas which evaporates during the process of distillation. So that
at the time of condensation, gas, ethanol it will melt back into the character of pure ethanol which is as
expected. In this it condenses in the condenser pipe which forms curved-curved spiral with a view to
expand the surface condenser when given the cold temperatures outside.
5. Reservoir bioethanol
As a tool storage container while bioethanol before used or packaged if used
commercially. Containers should be referred to requirements quality standards in order to stay
awake until the utilization of bioethanol, which includes the effect of temperature and pressure.
ISSN 2086-5953
Figure 5. Bioethanol production machines first stage
Description: 1. Vacuum pump
2. Condenser 3. Container bucket Bioethanol
4. Reactor + mixer 5. Waste extract window gelatin
6. Heater
2.2 Bioethanol Production Process
