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Simpulan Konstruksi dan Ekspresi Protein Fusi anti-EGFRvIII ..scFv::HPRmut ...sebagai kandidat imunotoksin pada Pichia ..pastoris

DAFTAR PUSTAKA Ahmad ZA, Yeap SK, Ali AM, Ho WY, Alitheen NBM, Hamid M. 2012. Review Article : scFv Antibodi: Principles and Clinical Application. Clin Develop Immunol Volume 2012, doi:10.11552012980250. Ardelt W, Ardelt B, Darzynkiewicz Z. Ribonucleases as potential modalities in anticancer therapy . 2009. European J of Pharmacol 625 181–189. Atalay G, Cardoso F, Awada A, Piccart MJ. 2003. Novel therapeutic strategies targeting the epidermal growth factor receptor EGFR family and its downstream effectors in breast cancer. Ann Oncol, 14: 1346–1363. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K editors. 2002. Short Protocol in Molecular Biology. 5 th Edition. John Wiley Sons Inc. Balamurugan V, Reddy GR, Suryanarayana VVS. 2007. Pichia pastoris: A notable heterologous expression system for the production of foreign proteins—Vaccines. Indian J Biotechnol, 6: 175-186. Bell P, Vandenberghe LH, Wu D, Johnston J, Limberis M, Wilson JM. 2007. A Comparative Analysis of Novel Fluorescent Proteins as Reporters for Gene Transfer Studies. J Histochem Cytochem. 559: 931–939 . DOI: 10.1369jhc.7A7180.2007. Benito A, Ribó M, Vilanova M. 2005. On the track of antitumour ribonucleases. Mol Biosyst

14: 294-302. doi: 10.1039B502847G.

Binyamin L, Borghaei H, Weiner LM. 2006. Cancer therapy with engineered monoclonal antibodies. Update on cancer therapeutics 1: 147–157. Bobrowicz P, Davidson RC, Li H, Potgieter TI, Nett JH, Hamilton SR, Stadheim TA, Miele RG, Bobrowicz B, Mitchell T, Rausch S, Renfer E, Wildt S. 2004. Engineering of an artificial glycosylation pathway blocked in core oligosaccharideassembly in the yeast Pichia pastoris: production of complex humanized glycoproteins with terminal galactose. Glycobiol 149: 757-766. doi:10.1093glycobcwh104. Bornhorst JA, Falke JJ. 2000. Purification of Proteins Using Polyhistidine Affinity Tags. Methods Enzymol, 326 : 245–254. Borriello M, Laccetti P, Terrazzano G, D’Alessio G, De Lorenzo C. 2011. A novel fully human antitumour immunoRNase targeting ErbB2-positive tumours. Br J Cancer 104, 1716 – 1723. Bretthauer RK. 2003. Genetic engineering of Pichia pastoris to humanize N- glycosylation of proteins. TRENDS Biotechnol 21 :11. Casey JL, Coley AM, Tilley LM, Foey M. 2000. Gren Fluorescent antibodies : Novel in vitro tools. Prot Eng 13 6 : 445-452.

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