53
3.75
Lampiran 11. Kadar air feses pada hari ke-13 dan hari ke-14
Kelompok perlakuan
Bobot Cawan Kosong W2 g
Bobot Sampel Sebelum
Dikeringkan W g
Bobot Sampel + Cawan Kering
W1 g Kadar Air
Feses Kadar
Air Feses
Rata- rata
Standar Deviasi
sd 1
2 1
2 1
2 1
2 Kontrol
negatif 4.6178
5.8039 0.2266
0.1481 4.7108
5.8730 58.96
53.34 56.15
0.30
5.8050 4.2449
0.1630 0.1118
5.8785 4.2935
54.91 56.53
55.72 Yogurt
sinbiotik 4.4655
0.2877 4.5817
59.61 59.61
5.09
4.4764 4.3613
0.1264 0.0710
4.5351 4.3959
53.56 51.27
52.41 Yogurt
sinbiotik dan EPEC
4.2345 5.7303
0.2290 0.1872
4.3180 5.7987
63.54 63.46
63.50
1.90
5.8086 4.4701
0.2182 0.2653
5.8799 4.5628
67.32 65.06
66.19 Kontrol
positif 4.2463
4.3610 0.5599
0.5207 4.4292
4.5432 67.33
65.01 66.17
0.98
4.4756 4.6207
0.3757 0.3792
4.6033 4.7378
66.01 69.12
67.56 Yogurt
prebiotik konvensio
nal 4.4687
4.4958 0.5778
0.5088 4.6918
4.6743 61.39
64.92 63.15
0.66
4.2470 4.5590
0.2060 0.1299
4.3125 4.6110
68.20 59.97
64.09
54
Lampiran 12. Uji statistik ANOVA dengan SPSS untuk kadar air feses tikus percobaan.
Tests of Between-Subjects Effects
Dependent Variable:Kadar_air_feses Source
Type III Sum of Squares
Df Mean Square
F Sig.
Model 37978.983
a
6 6329.831
834.048 .000
ulangan .681
1 .681
.090 .779
perlakuan 211.132
4 52.783
6.955 .043
Error 30.357
4 7.589
Total 38009.340
10 a. R Squared = .999 Adjusted R Squared = .998
Kadar_air_feses
Duncan Perlakuan
N Subset
1 2
kontrol negatif 2
55.9350 yogurt sinbiotik
2 56.0100
yogurt prebiotik 2
63.6200 63.6200
yogurt sinbiotik + EPEC 2
64.8450 kontrol positif
2 66.8650
Sig. .052
.310 Means for groups in homogeneous subsets are displayed.
Based on observed means. The error term is Mean SquareError = 7.589.
55
Lampiran 13. Uji statistika SPSS ANOVA pertumbuhan berat badan tikus percobaan
Tests of Between-Subjects Effects
Dependent Variable:Pertumbuhan_berat_badan Source
Type III Sum of Squares
df Mean Square
F Sig.
Model 2329.425
a
12 194.119
40.043 .000
Perlakuan 21.737
4 5.434
1.121 .367
Ulangan 329.024
7 47.003
9.696 .000
Error 135.738
28 4.848
Total 2465.162
40 a. R Squared = .945 Adjusted R Squared = .921
56
Lampiran 14. Contoh Perhitungan limfosit
Rumus menghitung jumlah selml :
FP = Faktor Pengenceran pengenceran yang dilakukan sewaktu perhitungan dengan tripan blue =2
Contoh Perhitungan pada sampel TO1 selml = 25112 x 2x 10
4
= 2.5111 × 10
7
selml = rata2 sel per bidang pandang x FP x 10
4
57
Lampiran 15. Data analisis limfosit
Kelompok perlakuan
Kode Limfosit Dua Bidang Pandang
Jumlah Limfosit ml ×10
7
Baseline TOU2
2511 2.511
TOU3 2393
2.393 TOU4
2112 2.112
Rata-Rata T0 2.3387
Kontrol negatif hari
ke-7 A1T1
4152 4.152
A3T1 4384
4.384 A4T1
4338 4.338
Rata-Rata AT1 4.291±0.12
Yogurt sinbiotik hari
ke-7 B1T1
7598 7.598
B3T1 7932
7.932 B5T1
8525 8.525
Rata-Rata BT1 8.018±0.47
Yogurt sinbiotik
+EPEC hari ke-7
C1T1 4806
4.806 C2T1
5962 5.962
C3T1 4224
4.224 Rata-Rata CT
4.997±0.88 Kontrol
positif hari ke-7
D1T1 4155
4.155 D2T1
5094 5.094
D3T1 4487
4.487 Rata-Rata DT1
4.579±0.48 Kontrol
negatif hari ke-14
A6T2 3313
3.313 A8T2
2827 2.827
A9T2 3062
3.062 Rata-Rata AT2
3.067±0.24 Yogurt
sinbiotik hari ke-14
B6T2 6827
6.827 B7T2
6686 6.686
B10T2 7511
7.511 Rata-Rata BT2
7.008±0.44 Yogurt
sinbiotik + EPEC hari
ke-14 C6T2
5368 5.368
C8T2 3696
3.696 C9T2
1907 1.907
Rata-Rata CT2 3.657±1.73
58
Lampiran 16. Uji statistik ANOVA dengan SPSS untuk limfosit hari ke-7
Tests of Between-Subjects Effects
Dependent Variable:limfosit_hari_7 Source
Type III Sum of Squares
df Mean Square
F Sig.
Model 3.869E16
a
6 6.448E15
246.695 .000
Ulangan 9.215E13
2 4.608E13
1.763 .250
kelompok_perlakuan 2.670E15
3 8.901E14
34.057 .000
Error 1.568E14
6 2.614E13
Total 3.884E16
12 a. R Squared = .996 Adjusted R Squared = .992
limfosit_hari_7
Duncan kelompok_perlakuan
N Subset
1 2
kontrol negatif 3
4.29E7 kontrol positif
3 4.58E7
yogurt sinbiotik +EPEC 3
5.00E7 yogurt sinbiotik
3 8.02E7
Sig. .153
1.000 Means for groups in homogeneous subsets are displayed.
Based on observed means. The error term is Mean SquareError = 26136358333333.330.
59
Lampiran 17. Uji statistik dengan SPSS untuk limfosit hari ke-14
Dependent Variable:jumlah_limfosit_hari_ke_14 Source
Type III Sum of Squares
Df Mean Square
F Sig.
Model 8.997E16
a
6 1.499E16
37.680 .000
ulangan 3.066E14
2 1.533E14
.385 .696
perlakuan 1.839E16
3 6.131E15
15.408 .003
Error 2.388E15
6 3.979E14
Total 9.236E16
12 a. R Squared = .974 Adjusted R Squared = .948
limfosit_hari_14
Duncan kelompok_perlakuan
N Subset
1 2
kontrol positif 3
1.68E7 kontrol negatif
3 3.07E7
yogurt sinbiotik + EPEC 3
3.66E7 yogurt sinbiotik
3 7.01E7
Sig. .058
1.000 Means for groups in homogeneous subsets are displayed.
Based on observed means. The error term is Mean SquareError = 99486997222222.200.
60
Lampiran 18. Uji Statistik dengan SPSS untuk Limfosit Hari ke-21
Tests of Between-Subjects Effects
Dependent Variable:limfosit_hari_21 Source
Type III Sum of Squares
df Mean Square
F Sig.
Model 1.825E16
a
7 2.607E15
6.377 .009
Ulangan 2.127E15
2 1.063E15
2.602 .135
kelompok_perlakuan 1.181E15
4 2.952E14
.722 .601
Error 3.270E15
8 4.087E14
Total 2.152E16
15 a. R Squared = .848 Adjusted R Squared = .715
61
Lampiran 19. Perhitungan kadar MDA 19.1. Kurva Standar
Larutan induk :Dilarutkan 30 µl standar TEP dengan air bebas ion hingga vol 50 ml 2.5 mmoll. Larutan kerja : Larutan induk sebanyak 50 µl lalu diencerkan sampai volume 5 ml 100 × dan larutan
ini siambil 1 ml lalu diencerkan 5 ml 5× sehingga konsentrasinya menjadi 5 µM. Dari kurva standar TEP diperoleh persamaan : Y = 0.238 +
0.016 X
19.2. Penentuan Konsentrasi MDA Contoh perhitungan untuk absorbansi MDA hati TO 2.