56.15 56.53 Yogurt 51.27 Yogurt 63.46 65.06 Kontrol 66.17 69.12 Yogurt 63.15 59.97

53 3.75 Lampiran 11. Kadar air feses pada hari ke-13 dan hari ke-14 Kelompok perlakuan Bobot Cawan Kosong W2 g Bobot Sampel Sebelum Dikeringkan W g Bobot Sampel + Cawan Kering W1 g Kadar Air Feses Kadar Air Feses Rata- rata Standar Deviasi sd 1 2 1 2 1 2 1 2 Kontrol negatif 4.6178 5.8039 0.2266 0.1481 4.7108 5.8730 58.96

53.34 56.15

0.30 5.8050 4.2449 0.1630 0.1118 5.8785 4.2935

54.91 56.53

55.72 Yogurt

sinbiotik 4.4655 0.2877 4.5817 59.61 59.61 5.09 4.4764 4.3613 0.1264 0.0710 4.5351 4.3959

53.56 51.27

52.41 Yogurt

sinbiotik dan EPEC 4.2345 5.7303 0.2290 0.1872 4.3180 5.7987

63.54 63.46

63.50 1.90 5.8086 4.4701 0.2182 0.2653 5.8799 4.5628

67.32 65.06

66.19 Kontrol

positif 4.2463 4.3610 0.5599 0.5207 4.4292 4.5432 67.33

65.01 66.17

0.98 4.4756 4.6207 0.3757 0.3792 4.6033 4.7378

66.01 69.12

67.56 Yogurt

prebiotik konvensio nal 4.4687 4.4958 0.5778 0.5088 4.6918 4.6743 61.39

64.92 63.15

0.66 4.2470 4.5590 0.2060 0.1299 4.3125 4.6110

68.20 59.97

64.09 54 Lampiran 12. Uji statistik ANOVA dengan SPSS untuk kadar air feses tikus percobaan. Tests of Between-Subjects Effects Dependent Variable:Kadar_air_feses Source Type III Sum of Squares Df Mean Square F Sig. Model 37978.983 a 6 6329.831 834.048 .000 ulangan .681 1 .681 .090 .779 perlakuan 211.132 4 52.783 6.955 .043 Error 30.357 4 7.589 Total 38009.340 10 a. R Squared = .999 Adjusted R Squared = .998 Kadar_air_feses Duncan Perlakuan N Subset 1 2 kontrol negatif 2 55.9350 yogurt sinbiotik 2 56.0100 yogurt prebiotik 2 63.6200 63.6200 yogurt sinbiotik + EPEC 2 64.8450 kontrol positif 2 66.8650 Sig. .052 .310 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean SquareError = 7.589. 55 Lampiran 13. Uji statistika SPSS ANOVA pertumbuhan berat badan tikus percobaan Tests of Between-Subjects Effects Dependent Variable:Pertumbuhan_berat_badan Source Type III Sum of Squares df Mean Square F Sig. Model 2329.425 a 12 194.119 40.043 .000 Perlakuan 21.737 4 5.434 1.121 .367 Ulangan 329.024 7 47.003 9.696 .000 Error 135.738 28 4.848 Total 2465.162 40 a. R Squared = .945 Adjusted R Squared = .921 56 Lampiran 14. Contoh Perhitungan limfosit Rumus menghitung jumlah selml : FP = Faktor Pengenceran pengenceran yang dilakukan sewaktu perhitungan dengan tripan blue =2 Contoh Perhitungan pada sampel TO1  selml = 25112 x 2x 10 4 = 2.5111 × 10 7  selml = rata2  sel per bidang pandang x FP x 10 4 57 Lampiran 15. Data analisis limfosit Kelompok perlakuan Kode Limfosit Dua Bidang Pandang Jumlah Limfosit ml ×10 7 Baseline TOU2 2511 2.511 TOU3 2393 2.393 TOU4 2112 2.112 Rata-Rata T0 2.3387 Kontrol negatif hari ke-7 A1T1 4152 4.152 A3T1 4384 4.384 A4T1 4338 4.338 Rata-Rata AT1 4.291±0.12 Yogurt sinbiotik hari ke-7 B1T1 7598 7.598 B3T1 7932 7.932 B5T1 8525 8.525 Rata-Rata BT1 8.018±0.47 Yogurt sinbiotik +EPEC hari ke-7 C1T1 4806 4.806 C2T1 5962 5.962 C3T1 4224 4.224 Rata-Rata CT 4.997±0.88 Kontrol positif hari ke-7 D1T1 4155 4.155 D2T1 5094 5.094 D3T1 4487 4.487 Rata-Rata DT1 4.579±0.48 Kontrol negatif hari ke-14 A6T2 3313 3.313 A8T2 2827 2.827 A9T2 3062 3.062 Rata-Rata AT2 3.067±0.24 Yogurt sinbiotik hari ke-14 B6T2 6827 6.827 B7T2 6686 6.686 B10T2 7511 7.511 Rata-Rata BT2 7.008±0.44 Yogurt sinbiotik + EPEC hari ke-14 C6T2 5368 5.368 C8T2 3696 3.696 C9T2 1907 1.907 Rata-Rata CT2 3.657±1.73 58 Lampiran 16. Uji statistik ANOVA dengan SPSS untuk limfosit hari ke-7 Tests of Between-Subjects Effects Dependent Variable:limfosit_hari_7 Source Type III Sum of Squares df Mean Square F Sig. Model 3.869E16 a 6 6.448E15 246.695 .000 Ulangan 9.215E13 2 4.608E13 1.763 .250 kelompok_perlakuan 2.670E15 3 8.901E14 34.057 .000 Error 1.568E14 6 2.614E13 Total 3.884E16 12 a. R Squared = .996 Adjusted R Squared = .992 limfosit_hari_7 Duncan kelompok_perlakuan N Subset 1 2 kontrol negatif 3 4.29E7 kontrol positif 3 4.58E7 yogurt sinbiotik +EPEC 3 5.00E7 yogurt sinbiotik 3 8.02E7 Sig. .153 1.000 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean SquareError = 26136358333333.330. 59 Lampiran 17. Uji statistik dengan SPSS untuk limfosit hari ke-14 Dependent Variable:jumlah_limfosit_hari_ke_14 Source Type III Sum of Squares Df Mean Square F Sig. Model 8.997E16 a 6 1.499E16 37.680 .000 ulangan 3.066E14 2 1.533E14 .385 .696 perlakuan 1.839E16 3 6.131E15 15.408 .003 Error 2.388E15 6 3.979E14 Total 9.236E16 12 a. R Squared = .974 Adjusted R Squared = .948 limfosit_hari_14 Duncan kelompok_perlakuan N Subset 1 2 kontrol positif 3 1.68E7 kontrol negatif 3 3.07E7 yogurt sinbiotik + EPEC 3 3.66E7 yogurt sinbiotik 3 7.01E7 Sig. .058 1.000 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean SquareError = 99486997222222.200. 60 Lampiran 18. Uji Statistik dengan SPSS untuk Limfosit Hari ke-21 Tests of Between-Subjects Effects Dependent Variable:limfosit_hari_21 Source Type III Sum of Squares df Mean Square F Sig. Model 1.825E16 a 7 2.607E15 6.377 .009 Ulangan 2.127E15 2 1.063E15 2.602 .135 kelompok_perlakuan 1.181E15 4 2.952E14 .722 .601 Error 3.270E15 8 4.087E14 Total 2.152E16 15 a. R Squared = .848 Adjusted R Squared = .715 61 Lampiran 19. Perhitungan kadar MDA 19.1. Kurva Standar Larutan induk :Dilarutkan 30 µl standar TEP dengan air bebas ion hingga vol 50 ml 2.5 mmoll. Larutan kerja : Larutan induk sebanyak 50 µl lalu diencerkan sampai volume 5 ml 100 × dan larutan ini siambil 1 ml lalu diencerkan 5 ml 5× sehingga konsentrasinya menjadi 5 µM. Dari kurva standar TEP diperoleh persamaan : Y = 0.238 + 0.016 X

19.2. Penentuan Konsentrasi MDA Contoh perhitungan untuk absorbansi MDA hati TO 2.